LOCUS       NM_176861               2460 bp    mRNA    linear   ROD 14-FEB-2025
DEFINITION  Rattus norvegicus potassium calcium-activated channel subfamily M
            regulatory beta subunit 2 (Kcnmb2), mRNA.
ACCESSION   NM_176861 XM_017590735
VERSION     NM_176861.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 2460)
  AUTHORS   Bukiya,A.N., McMillan,J.E., Fedinec,A.L., Patil,S.A., Miller,D.D.,
            Leffler,C.W., Parrill,A.L. and Dopico,A.M.
  TITLE     Cerebrovascular dilation via selective targeting of the cholane
            steroid-recognition site in the BK channel beta1-subunit by a novel
            nonsteroidal agent
  JOURNAL   Mol Pharmacol 83 (5), 1030-1044 (2013)
   PUBMED   23455312
REFERENCE   2  (bases 1 to 2460)
  AUTHORS   Li,W., Gao,S.B., Lv,C.X., Wu,Y., Guo,Z.H., Ding,J.P. and Xu,T.
  TITLE     Characterization of voltage-and Ca2+-activated K+ channels in rat
            dorsal root ganglion neurons
  JOURNAL   J Cell Physiol 212 (2), 348-357 (2007)
   PUBMED   17523149
  REMARK    GeneRIF: Kcnmb2 is responsible for inactivating large-conductance
            calcium-dependent potassium currents in small sensory dorsal root
            ganglion neurons.
REFERENCE   3  (bases 1 to 2460)
  AUTHORS   Morimoto,T., Sakamoto,K., Sade,H., Ohya,S., Muraki,K. and
            Imaizumi,Y.
  TITLE     Voltage-sensitive oxonol dyes are novel large-conductance
            Ca2+-activated K+ channel activators selective for beta1 and beta4
            but not for beta2 subunits
  JOURNAL   Mol Pharmacol 71 (4), 1075-1088 (2007)
   PUBMED   17209121
  REMARK    GeneRIF: DiBAC(4)(3) activated whole-cell BKalphabeta1 and
            BKalphabeta4 currents in the same concentration range but partially
            blocked BKalphabeta2 currents. Structure activity relationships are
            discussed.
REFERENCE   4  (bases 1 to 2460)
  AUTHORS   Langer,P., Grunder,S. and Rusch,A.
  TITLE     Expression of Ca2+-activated BK channel mRNA and its splice
            variants in the rat cochlea
  JOURNAL   J Comp Neurol 455 (2), 198-209 (2003)
   PUBMED   12454985
REFERENCE   5  (bases 1 to 2460)
  AUTHORS   Brenner,R., Jegla,T.J., Wickenden,A., Liu,Y. and Aldrich,R.W.
  TITLE     Cloning and functional characterization of novel large conductance
            calcium-activated potassium channel beta subunits, hKCNMB3 and
            hKCNMB4
  JOURNAL   J Biol Chem 275 (9), 6453-6461 (2000)
   PUBMED   10692449
REFERENCE   6  (bases 1 to 2460)
  AUTHORS   Xia,X.M., Ding,J.P. and Lingle,C.J.
  TITLE     Molecular basis for the inactivation of Ca2+- and voltage-dependent
            BK channels in adrenal chromaffin cells and rat insulinoma tumor
            cells
  JOURNAL   J Neurosci 19 (13), 5255-5264 (1999)
   PUBMED   10377337
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000002.1.
            
            On or before Oct 4, 2022 this sequence version replaced
            XM_017590735.2, NM_176861.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR22582424.705264.1,
                                           SRR22582424.2194427.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760433, SAMN06621351
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-275               JAXUCZ010000002.1  116599299-116599573
            276-398             JAXUCZ010000002.1  116864474-116864596
            399-569             JAXUCZ010000002.1  116880296-116880466
            570-765             JAXUCZ010000002.1  116883175-116883370
            766-2460            JAXUCZ010000002.1  116902160-116903854
FEATURES             Location/Qualifiers
     source          1..2460
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="2"
                     /map="2q24"
     gene            1..2460
                     /gene="Kcnmb2"
                     /gene_synonym="Kcmb2"
                     /note="potassium calcium-activated channel subfamily M
                     regulatory beta subunit 2"
                     /db_xref="GeneID:294961"
                     /db_xref="RGD:631398"
     exon            1..275
                     /gene="Kcnmb2"
                     /gene_synonym="Kcmb2"
                     /inference="alignment:Splign:2.1.0"
     exon            276..398
                     /gene="Kcnmb2"
                     /gene_synonym="Kcmb2"
                     /inference="alignment:Splign:2.1.0"
     CDS             343..1050
                     /gene="Kcnmb2"
                     /gene_synonym="Kcmb2"
                     /note="rbeta3; BKbeta2; slo-beta-2; k(VCA)beta-2; BK
                     channel subunit beta-2; maxi K channel subunit beta-2;
                     charybdotoxin receptor subunit beta-2; calcium-activated
                     potassium channel, subfamily M subunit beta-2;
                     calcium-activated potassium channel beta 2; potassium
                     large conductance calcium-activated channel, subfamily M,
                     beta member 2; potassium channel subfamily M regulatory
                     beta subunit 2"
                     /codon_start=1
                     /product="calcium-activated potassium channel subunit
                     beta-2"
                     /protein_id="NP_789831.2"
                     /db_xref="GeneID:294961"
                     /db_xref="RGD:631398"
                     /translation="MFIWTSGRTSSSYRHDEKRNIYQKIRDHDLLDKRKTVTALKAGE
                     DRAILLGLAMMVCSIMMYFLLGITLLRSYMQSVWTEEAQCALLNVSITETFNCSFSCG
                     PDCWKLSQYPCLQVYVNLTSSGEKLLLYHTEETMKINQKCSYIPKCGNNFEESMSLVS
                     VVMENFRRHQHFPCYSDPEGNQKSVILTKLYSSNVLFHSLFWPTCMMAGGVAIVAMVK
                     LTQYLSLLCERIQRINR"
     exon            399..569
                     /gene="Kcnmb2"
                     /gene_synonym="Kcmb2"
                     /inference="alignment:Splign:2.1.0"
     exon            570..765
                     /gene="Kcnmb2"
                     /gene_synonym="Kcmb2"
                     /inference="alignment:Splign:2.1.0"
     exon            766..2460
                     /gene="Kcnmb2"
                     /gene_synonym="Kcmb2"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 agtctgaact agcgtccacg gagcttctga gaactaatct taagcatggc tgtctagcgt
       61 gcctgtcact cctattgtcc ttccaaactc ttgcagacat tttgagcgag gagtattaaa
      121 gctatgagtt agcgagggtg tgacatcaat ggtccacaaa ggctgccagc aggaaaggta
      181 aagatgagtc ccggtggctg tgtggaggac tacacgggat cgctgtcatt aaaaattaag
      241 cgtggctttt gaggaagatg tgacaactac cggaggtctt tccgccactc ccccaggact
      301 tccacagcaa ggacaggagc tgcaggacca acatcctcta agatgtttat atggaccagt
      361 ggccggacct cttcatctta cagacacgac gagaaaagaa atatctacca gaaaatcagg
      421 gaccatgacc tcctggacaa aaggaaaact gtgacagctc tgaaggctgg ggaggaccgg
      481 gccatcctgc ttggactggc catgatggtg tgctccatca tgatgtactt cctactggga
      541 atcacactgc tgcgctcgta catgcagagt gtatggacag aagaagccca gtgtgccctg
      601 ctgaatgtgt caatcacaga aacatttaac tgttccttca gctgtgggcc tgactgctgg
      661 aagctctctc agtacccttg cctgcaggta tacgtgaacc tgacatcttc tggggagaag
      721 ctcctcctct accacacaga agagaccatg aagatcaatc aaaagtgctc ctatattcct
      781 aagtgtggaa acaactttga ggagtccatg tcccttgtga gtgtcgtcat ggaaaacttc
      841 aggagacacc aacacttccc ctgctattct gacccagaag ggaaccaaaa gagcgtcatc
      901 ctgaccaaac tctatagctc caatgtgctg ttccattctc tcttctggcc aacgtgtatg
      961 atggctgggg gtgtggcaat cgttgctatg gtgaaactaa ctcagtacct ctccctgctt
     1021 tgtgagagga tccaacggat caacagataa aagaaaaaaa acacatggat aagatatttt
     1081 tctttaaagc tcaaatactg ttttctttcg ttcttcacca aagaacctta agtttgtaat
     1141 gtgcagtctg ttatgggttc cttaatatat tattctatgt agagcaataa cgcaaacgct
     1201 gtcctatatg caaacatgat gtccttattt attcaggaga attaactgtt cctcattggt
     1261 cggtcgtttc attatattgt tttgatgctg gaactagtct gccctctttc caccagagta
     1321 gggctcagct actcattacc aagtgttgtg gggggaatgg aggcactgtc cccaagacca
     1381 ggcctgtgtt ctgagtttct agaattcatg aagacaagct ttttcttcat ttgttgttta
     1441 ctgaagctac agccaaaaat gcattttttt cttttcttaa tctacattga gccctgtagc
     1501 aagtggaggg gttgtttcct gactaaaggg tgttatacct ttttttaaaa ttattattat
     1561 tattattatt atttttacca cagcactgta aggaagagag gaaatctagc cagcaatctt
     1621 tctttcatca cttttcattc acaaattcag atttgtttga ataattatca actctataat
     1681 gtcttccgaa cagagtcctt ttccatctat gaccctgcta ctgtgtggaa gaccaagctg
     1741 gtgagatcac tcccagtgaa ctgactgggt gttggaaggt atgccattgc aattttggaa
     1801 agtctgtgtt acaaagatat ttatgtgaaa tcttaactcc atttccactg gactgttaga
     1861 agcaaatatt aatacactaa atactggtga actggttgca atgagtgagt ctgtcagggc
     1921 cagctatcat gttggaatat tactctttgg gaatttgtgt ttcagtaaac gaatgtacag
     1981 tctctccttc tgaaaaaaaa aatgttgcct gttgaggttt taaatctatg tgtgcagaat
     2041 ctttgtctcc tctacatatt tttatttttc tactcactat tcccttttgg ttgttatatt
     2101 ttatatgcag aatagacctt tttcctaaca catgtttgaa atgaataata ggtttaagag
     2161 aagcctttct tcacattgat gtttactcat tctgtttggt ggggggataa ggggttggtt
     2221 ttaaataaag acattctgtc ctttatttaa tatcgatatc taagggagaa gacaaacatc
     2281 tatctttctc atctatattt aagtaccctt ttgtaacata gtatcgatgt tttttaggta
     2341 attgcaaaat tttacaaatc atttgtgaaa tgaatggtga aacttatctg aggaagcaga
     2401 atattgtaat tcatagtctg acttttcata agcaaataaa tccctagggt gtaatcagga
//
