LOCUS       NM_001393699           11408 bp    mRNA    linear   ROD 12-JUN-2024
DEFINITION  Rattus norvegicus potassium calcium-activated channel subfamily M
            alpha 1 (Kcnma1), transcript variant 2, mRNA.
ACCESSION   NM_001393699
VERSION     NM_001393699.1
KEYWORDS    RefSeq.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 11408)
  AUTHORS   Akdag,M.Z., Ogras,E., Doganyigit,Z., Akyuz,E., Akdag,M.B., Okan,A.,
            Akpolat,V. and Kullu,I.R.
  TITLE     The increase in c-fos expression in epileptic seizures is inhibited
            by magnetic field application, but not KCa1.1 channel expression
  JOURNAL   Electromagn Biol Med 42 (2), 81-97 (2023)
   PUBMED   37598353
  REMARK    GeneRIF: The increase in c-fos expression in epileptic seizures is
            inhibited by magnetic field application, but not KCa1.1 channel
            expression.
REFERENCE   2  (bases 1 to 11408)
  AUTHORS   Yin,H., Cheng,H., Li,P. and Yang,Z.
  TITLE     TRPC6 interacted with KCa1.1 channels to regulate the proliferation
            and apoptosis of glioma cells
  JOURNAL   Arch Biochem Biophys 725, 109268 (2022)
   PUBMED   35489424
  REMARK    GeneRIF: TRPC6 interacted with KCa1.1 channels to regulate the
            proliferation and apoptosis of glioma cells.
REFERENCE   3  (bases 1 to 11408)
  AUTHORS   Al-Dhuhli,F., Al-Siyabi,S., Al-Maamari,H., Al-Farsi,S. and
            Albarwani,S.
  TITLE     Moderate-intensity exercise training reduces vasorelaxation of
            mesenteric arteries: role of BKCa channels and nitric oxide
  JOURNAL   Physiol Res 71 (1), 67-77 (2022)
   PUBMED   35043645
  REMARK    GeneRIF: Moderate-intensity exercise training reduces
            vasorelaxation of mesenteric arteries: role of BKCa channels and
            nitric oxide.
REFERENCE   4  (bases 1 to 11408)
  AUTHORS   Wang,L.Q., Qian,Z., Ma,H.L., Zhou,M., Li,H.D., Cui,C.P., Luo,D.L.,
            Li,X.L. and Li,B.Y.
  TITLE     Estrogen-dependent KCa1.1 modulation is essential for retaining
            neuroexcitation of female-specific subpopulation of myelinated
            Ah-type baroreceptor neurons in rats
  JOURNAL   Acta Pharmacol Sin 42 (12), 2173-2180 (2021)
   PUBMED   34267344
  REMARK    GeneRIF: Estrogen-dependent KCa1.1 modulation is essential for
            retaining neuroexcitation of female-specific subpopulation of
            myelinated Ah-type baroreceptor neurons in rats.
REFERENCE   5  (bases 1 to 11408)
  AUTHORS   He,C., Li,X., Wang,M., Zhang,S. and Liu,H.
  TITLE     Deletion of BK channels decreased skeletal and cardiac muscle
            function but increased smooth muscle contraction in rats
  JOURNAL   Biochem Biophys Res Commun 570, 8-14 (2021)
   PUBMED   34271438
  REMARK    GeneRIF: Deletion of BK channels decreased skeletal and cardiac
            muscle function but increased smooth muscle contraction in rats.
REFERENCE   6  (bases 1 to 11408)
  AUTHORS   Liu,Y., Pleyte,K., Knaus,H.G. and Rusch,N.J.
  TITLE     Increased expression of Ca2+-sensitive K+ channels in aorta of
            hypertensive rats
  JOURNAL   Hypertension 30 (6), 1403-1409 (1997)
   PUBMED   9403560
REFERENCE   7  (bases 1 to 11408)
  AUTHORS   McCobb,D.P., Fowler,N.L., Featherstone,T., Lingle,C.J., Saito,M.,
            Krause,J.E. and Salkoff,L.
  TITLE     A human calcium-activated potassium channel gene expressed in
            vascular smooth muscle
  JOURNAL   Am J Physiol 269 (3 Pt 2), H767-H777 (1995)
   PUBMED   7573516
REFERENCE   8  (bases 1 to 11408)
  AUTHORS   Tseng-Crank,J., Foster,C.D., Krause,J.D., Mertz,R., Godinot,N.,
            DiChiara,T.J. and Reinhart,P.H.
  TITLE     Cloning, expression, and distribution of functionally distinct
            Ca(2+)-activated K+ channel isoforms from human brain
  JOURNAL   Neuron 13 (6), 1315-1330 (1994)
   PUBMED   7993625
REFERENCE   9  (bases 1 to 11408)
  AUTHORS   Dworetzky,S.I., Trojnacki,J.T. and Gribkoff,V.K.
  TITLE     Cloning and expression of a human large-conductance
            calcium-activated potassium channel
  JOURNAL   Brain Res Mol Brain Res 27 (1), 189-193 (1994)
   PUBMED   7877450
REFERENCE   10 (bases 1 to 11408)
  AUTHORS   Butler,A., Tsunoda,S., McCobb,D.P., Wei,A. and Salkoff,L.
  TITLE     mSlo, a complex mouse gene encoding 'maxi' calcium-activated
            potassium channels
  JOURNAL   Science 261 (5118), 221-224 (1993)
   PUBMED   7687074
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000015.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF135265.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN12840105, SAMN12840106
                                           [ECO:0000348]
            ##Evidence-Data-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-458               JAXUCZ010000015.1  351925-352382
            459-620             JAXUCZ010000015.1  547266-547427
            621-682             JAXUCZ010000015.1  700391-700452
            683-776             JAXUCZ010000015.1  759245-759338
            777-888             JAXUCZ010000015.1  760226-760337
            889-964             JAXUCZ010000015.1  806968-807043
            965-1040            JAXUCZ010000015.1  815927-816002
            1041-1211           JAXUCZ010000015.1  817284-817454
            1212-1303           JAXUCZ010000015.1  819061-819152
            1304-1414           JAXUCZ010000015.1  834623-834733
            1415-1520           JAXUCZ010000015.1  836417-836522
            1521-1603           JAXUCZ010000015.1  839136-839218
            1604-1673           JAXUCZ010000015.1  843160-843229
            1674-1829           JAXUCZ010000015.1  849837-849992
            1830-1939           JAXUCZ010000015.1  884108-884217
            1940-2008           JAXUCZ010000015.1  895350-895418
            2009-2020           JAXUCZ010000015.1  897759-897770
            2021-2107           JAXUCZ010000015.1  903992-904078
            2108-2184           JAXUCZ010000015.1  910735-910811
            2185-2278           JAXUCZ010000015.1  951067-951160
            2279-2402           JAXUCZ010000015.1  964179-964302
            2403-2627           JAXUCZ010000015.1  969376-969600
            2628-2820           JAXUCZ010000015.1  973116-973308
            2821-2934           JAXUCZ010000015.1  1000979-1001092
            2935-3015           JAXUCZ010000015.1  1001900-1001980
            3016-3146           JAXUCZ010000015.1  1007146-1007276
            3147-3341           JAXUCZ010000015.1  1035923-1036117
            3342-3460           JAXUCZ010000015.1  1038511-1038629
            3461-3685           JAXUCZ010000015.1  1040102-1040326
            3686-11408          JAXUCZ010000015.1  1049395-1057117
FEATURES             Location/Qualifiers
     source          1..11408
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="15"
                     /map="15p16"
     gene            1..11408
                     /gene="Kcnma1"
                     /gene_synonym="BKCa; BKCA alpha; cbv1; k(VCA)alpha;
                     KCa1.1; Kcnma; KCNMA1b; KCNMA1c; maxiK; Slo; slo-alpha;
                     slo1"
                     /note="potassium calcium-activated channel subfamily M
                     alpha 1"
                     /db_xref="GeneID:83731"
                     /db_xref="RGD:620715"
     exon            1..458
                     /gene="Kcnma1"
                     /gene_synonym="BKCa; BKCA alpha; cbv1; k(VCA)alpha;
                     KCa1.1; Kcnma; KCNMA1b; KCNMA1c; maxiK; Slo; slo-alpha;
                     slo1"
                     /inference="alignment:Splign:2.1.0"
     CDS             81..3710
                     /gene="Kcnma1"
                     /gene_synonym="BKCa; BKCA alpha; cbv1; k(VCA)alpha;
                     KCa1.1; Kcnma; KCNMA1b; KCNMA1c; maxiK; Slo; slo-alpha;
                     slo1"
                     /note="isoform 2 is encoded by transcript variant 2;
                     calcium-activated potassium channel subunit alpha-1;
                     calcium-activated potassium channel alpha subunit; BK
                     channel; slo homolog; maxi K channel; slowpoke homolog;
                     calcium-activated potassium channel, subfamily M subunit
                     alpha-1; potassium large conductance calcium-activated
                     channel, subfamily M, alpha member 1; potassium channel,
                     calcium activated large conductance subfamily M alpha,
                     member 1"
                     /codon_start=1
                     /product="calcium-activated potassium channel subunit
                     alpha-1 isoform 2"
                     /protein_id="NP_001380628.1"
                     /db_xref="GeneID:83731"
                     /db_xref="RGD:620715"
                     /translation="MANGGGGGGGGSSGSSGGGGGGGGGSGLRMSSNIHANHLSLDAS
                     SSSSSSSSSSSSSSSSVHEPKMDALIIPVTMEVPCDSRGQRMWWAFLASSMVTFFGGL
                     FIILLWRTLKYLWTVCCHCGGKTKEAQKINNGSSQADGTLKPVDEKEEVVAAEVGWMT
                     SVKDWAGVMISAQTLTGRVLVVLVFALSIGALVIYFIDSSNPIESCQNFYKDFTLQID
                     MAFNVFFLLYFGLRFIAANDKLWFWLEVNSVVDFFTVPPVFVSVYLNRSWLGLRFLRA
                     LRLIQFSEILQFLNILKTSNSIKLVNLLSIFISTWLTAAGFIHLVENSGDPWENFQNN
                     QALTYWECVYLLMVTMSTVGYGDVYAKTTLGRLFMVFFILGGLAMFASYVPEIIELIG
                     NRKKYGGSYSAVSGRKHIVVCGHITLESVSNFLKDFLHKDRDDVNVEIVFLHNISPNL
                     ELEALFKRHFTQVEFYQGSVLNPHDLARVKIESADACLILANKYCADPDAEDASNIMR
                     VISIKNYHPKIRIITQMLQYHNKAHLLNIPSWNWKEGDDAICLAELKLGFIAQSCLAQ
                     GLSTMLANLFSMRSFIKIEEDTWQKYYLEGVSNEMYTEYLSSAFVGLSFPTVCELCFV
                     KLKLLMIAIEYKSANRESRSRKRILINPGNHLKIQEGTLGFFIASDAKEVKRAFFYCK
                     ACHDDVTDPKRIKKCGCRRLEDEQPPTLSPKKKQRNGGMRNSPNTSPKLMRHDPLLIP
                     GNDQIDNMDSNVKKYDSTGMFHWCAPKEIEKVILTRSEAAMTVLSGHVVVCIFGDVSS
                     ALIGLRNLVMPLRASNFHYHELKHIVFVGSIEYLKREWETLHNFPKVSILPGTPLSRA
                     DLRAVNINLCDMCVILSANQNNIDDTSLQDKECILASLNIKSMQFDDSIGVLQANSQG
                     FTPPGMDRSSPDNSPVHGMLRQPSITTGVNIPIITELAKPGKLPLVSVNQEKNSGTHI
                     LMITELVNDTNVQFLDQDDDDDPDTELYLTQPFACGTAFAVSVLDSLMSATYFNDNIL
                     TLIRTLVTGGATPELEALIAEENALRGGYSTPQTLANRDRCRVAQLALLDGPFADLGD
                     GGCYGDLFCKALKTYNMLCFGIYRLRDAHLSTPSQCTKRYVITNPPYEFELVPTDLIF
                     CLMQFDHNAGQSRASLSHSSHSSQSSSKKSSSVHSIPSTANRPNRPKSRESRDKQNRK
                     EMVYR"
     exon            459..620
                     /gene="Kcnma1"
                     /gene_synonym="BKCa; BKCA alpha; cbv1; k(VCA)alpha;
                     KCa1.1; Kcnma; KCNMA1b; KCNMA1c; maxiK; Slo; slo-alpha;
                     slo1"
                     /inference="alignment:Splign:2.1.0"
     exon            621..682
                     /gene="Kcnma1"
                     /gene_synonym="BKCa; BKCA alpha; cbv1; k(VCA)alpha;
                     KCa1.1; Kcnma; KCNMA1b; KCNMA1c; maxiK; Slo; slo-alpha;
                     slo1"
                     /inference="alignment:Splign:2.1.0"
     exon            683..776
                     /gene="Kcnma1"
                     /gene_synonym="BKCa; BKCA alpha; cbv1; k(VCA)alpha;
                     KCa1.1; Kcnma; KCNMA1b; KCNMA1c; maxiK; Slo; slo-alpha;
                     slo1"
                     /inference="alignment:Splign:2.1.0"
     exon            777..888
                     /gene="Kcnma1"
                     /gene_synonym="BKCa; BKCA alpha; cbv1; k(VCA)alpha;
                     KCa1.1; Kcnma; KCNMA1b; KCNMA1c; maxiK; Slo; slo-alpha;
                     slo1"
                     /inference="alignment:Splign:2.1.0"
     exon            889..964
                     /gene="Kcnma1"
                     /gene_synonym="BKCa; BKCA alpha; cbv1; k(VCA)alpha;
                     KCa1.1; Kcnma; KCNMA1b; KCNMA1c; maxiK; Slo; slo-alpha;
                     slo1"
                     /inference="alignment:Splign:2.1.0"
     exon            965..1040
                     /gene="Kcnma1"
                     /gene_synonym="BKCa; BKCA alpha; cbv1; k(VCA)alpha;
                     KCa1.1; Kcnma; KCNMA1b; KCNMA1c; maxiK; Slo; slo-alpha;
                     slo1"
                     /inference="alignment:Splign:2.1.0"
     exon            1041..1211
                     /gene="Kcnma1"
                     /gene_synonym="BKCa; BKCA alpha; cbv1; k(VCA)alpha;
                     KCa1.1; Kcnma; KCNMA1b; KCNMA1c; maxiK; Slo; slo-alpha;
                     slo1"
                     /inference="alignment:Splign:2.1.0"
     exon            1212..1303
                     /gene="Kcnma1"
                     /gene_synonym="BKCa; BKCA alpha; cbv1; k(VCA)alpha;
                     KCa1.1; Kcnma; KCNMA1b; KCNMA1c; maxiK; Slo; slo-alpha;
                     slo1"
                     /inference="alignment:Splign:2.1.0"
     exon            1304..1414
                     /gene="Kcnma1"
                     /gene_synonym="BKCa; BKCA alpha; cbv1; k(VCA)alpha;
                     KCa1.1; Kcnma; KCNMA1b; KCNMA1c; maxiK; Slo; slo-alpha;
                     slo1"
                     /inference="alignment:Splign:2.1.0"
     exon            1415..1520
                     /gene="Kcnma1"
                     /gene_synonym="BKCa; BKCA alpha; cbv1; k(VCA)alpha;
                     KCa1.1; Kcnma; KCNMA1b; KCNMA1c; maxiK; Slo; slo-alpha;
                     slo1"
                     /inference="alignment:Splign:2.1.0"
     exon            1521..1603
                     /gene="Kcnma1"
                     /gene_synonym="BKCa; BKCA alpha; cbv1; k(VCA)alpha;
                     KCa1.1; Kcnma; KCNMA1b; KCNMA1c; maxiK; Slo; slo-alpha;
                     slo1"
                     /inference="alignment:Splign:2.1.0"
     exon            1604..1673
                     /gene="Kcnma1"
                     /gene_synonym="BKCa; BKCA alpha; cbv1; k(VCA)alpha;
                     KCa1.1; Kcnma; KCNMA1b; KCNMA1c; maxiK; Slo; slo-alpha;
                     slo1"
                     /inference="alignment:Splign:2.1.0"
     exon            1674..1829
                     /gene="Kcnma1"
                     /gene_synonym="BKCa; BKCA alpha; cbv1; k(VCA)alpha;
                     KCa1.1; Kcnma; KCNMA1b; KCNMA1c; maxiK; Slo; slo-alpha;
                     slo1"
                     /inference="alignment:Splign:2.1.0"
     exon            1830..1939
                     /gene="Kcnma1"
                     /gene_synonym="BKCa; BKCA alpha; cbv1; k(VCA)alpha;
                     KCa1.1; Kcnma; KCNMA1b; KCNMA1c; maxiK; Slo; slo-alpha;
                     slo1"
                     /inference="alignment:Splign:2.1.0"
     exon            1940..2008
                     /gene="Kcnma1"
                     /gene_synonym="BKCa; BKCA alpha; cbv1; k(VCA)alpha;
                     KCa1.1; Kcnma; KCNMA1b; KCNMA1c; maxiK; Slo; slo-alpha;
                     slo1"
                     /inference="alignment:Splign:2.1.0"
     exon            2009..2020
                     /gene="Kcnma1"
                     /gene_synonym="BKCa; BKCA alpha; cbv1; k(VCA)alpha;
                     KCa1.1; Kcnma; KCNMA1b; KCNMA1c; maxiK; Slo; slo-alpha;
                     slo1"
                     /inference="alignment:Splign:2.1.0"
     exon            2021..2107
                     /gene="Kcnma1"
                     /gene_synonym="BKCa; BKCA alpha; cbv1; k(VCA)alpha;
                     KCa1.1; Kcnma; KCNMA1b; KCNMA1c; maxiK; Slo; slo-alpha;
                     slo1"
                     /inference="alignment:Splign:2.1.0"
     exon            2108..2184
                     /gene="Kcnma1"
                     /gene_synonym="BKCa; BKCA alpha; cbv1; k(VCA)alpha;
                     KCa1.1; Kcnma; KCNMA1b; KCNMA1c; maxiK; Slo; slo-alpha;
                     slo1"
                     /inference="alignment:Splign:2.1.0"
     exon            2185..2278
                     /gene="Kcnma1"
                     /gene_synonym="BKCa; BKCA alpha; cbv1; k(VCA)alpha;
                     KCa1.1; Kcnma; KCNMA1b; KCNMA1c; maxiK; Slo; slo-alpha;
                     slo1"
                     /inference="alignment:Splign:2.1.0"
     exon            2279..2402
                     /gene="Kcnma1"
                     /gene_synonym="BKCa; BKCA alpha; cbv1; k(VCA)alpha;
                     KCa1.1; Kcnma; KCNMA1b; KCNMA1c; maxiK; Slo; slo-alpha;
                     slo1"
                     /inference="alignment:Splign:2.1.0"
     exon            2403..2627
                     /gene="Kcnma1"
                     /gene_synonym="BKCa; BKCA alpha; cbv1; k(VCA)alpha;
                     KCa1.1; Kcnma; KCNMA1b; KCNMA1c; maxiK; Slo; slo-alpha;
                     slo1"
                     /inference="alignment:Splign:2.1.0"
     exon            2628..2820
                     /gene="Kcnma1"
                     /gene_synonym="BKCa; BKCA alpha; cbv1; k(VCA)alpha;
                     KCa1.1; Kcnma; KCNMA1b; KCNMA1c; maxiK; Slo; slo-alpha;
                     slo1"
                     /inference="alignment:Splign:2.1.0"
     exon            2821..2934
                     /gene="Kcnma1"
                     /gene_synonym="BKCa; BKCA alpha; cbv1; k(VCA)alpha;
                     KCa1.1; Kcnma; KCNMA1b; KCNMA1c; maxiK; Slo; slo-alpha;
                     slo1"
                     /inference="alignment:Splign:2.1.0"
     exon            2935..3015
                     /gene="Kcnma1"
                     /gene_synonym="BKCa; BKCA alpha; cbv1; k(VCA)alpha;
                     KCa1.1; Kcnma; KCNMA1b; KCNMA1c; maxiK; Slo; slo-alpha;
                     slo1"
                     /inference="alignment:Splign:2.1.0"
     exon            3016..3146
                     /gene="Kcnma1"
                     /gene_synonym="BKCa; BKCA alpha; cbv1; k(VCA)alpha;
                     KCa1.1; Kcnma; KCNMA1b; KCNMA1c; maxiK; Slo; slo-alpha;
                     slo1"
                     /inference="alignment:Splign:2.1.0"
     exon            3147..3341
                     /gene="Kcnma1"
                     /gene_synonym="BKCa; BKCA alpha; cbv1; k(VCA)alpha;
                     KCa1.1; Kcnma; KCNMA1b; KCNMA1c; maxiK; Slo; slo-alpha;
                     slo1"
                     /inference="alignment:Splign:2.1.0"
     exon            3342..3460
                     /gene="Kcnma1"
                     /gene_synonym="BKCa; BKCA alpha; cbv1; k(VCA)alpha;
                     KCa1.1; Kcnma; KCNMA1b; KCNMA1c; maxiK; Slo; slo-alpha;
                     slo1"
                     /inference="alignment:Splign:2.1.0"
     exon            3461..3685
                     /gene="Kcnma1"
                     /gene_synonym="BKCa; BKCA alpha; cbv1; k(VCA)alpha;
                     KCa1.1; Kcnma; KCNMA1b; KCNMA1c; maxiK; Slo; slo-alpha;
                     slo1"
                     /inference="alignment:Splign:2.1.0"
     exon            3686..11408
                     /gene="Kcnma1"
                     /gene_synonym="BKCa; BKCA alpha; cbv1; k(VCA)alpha;
                     KCa1.1; Kcnma; KCNMA1b; KCNMA1c; maxiK; Slo; slo-alpha;
                     slo1"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gctgttgatg ggtgttcggg gtggcgctgg cggcgggagg agctccctcg agcccctgcg
       61 ccggccgtcc gttgctagct atggcaaacg gtggcggcgg cggcggcggc ggcagcagcg
      121 gcagcagcgg cggcggcggc ggcggcggcg gaggcagcgg tcttagaatg agcagcaata
      181 tccacgcgaa ccatctcagc ctagacgcgt cctcttcttc ctcctcctct tcttcctcct
      241 cctcttcctc ctcgtcctcg gtccacgagc ccaagatgga tgcgctcatc atcccggtga
      301 ccatggaggt gccgtgcgac agccggggcc aacgcatgtg gtgggctttc ttggcctcct
      361 ccatggtgac tttcttcggg ggcctcttca tcatcttgct ctggcggact ctcaagtacc
      421 tgtggaccgt ttgctgccac tgcgggggca agacgaagga ggcccagaag ataaacaatg
      481 gctccagcca ggcagatggt actctcaagc cagtggatga aaaagaggag gtggtggcag
      541 ccgaggtcgg ctggatgaca tctgtgaagg actgggcagg ggtgatgata tctgcccaga
      601 cactgactgg cagagtcctg gttgtgttag tctttgctct cagcattggt gcccttgtaa
      661 tatacttcat agactcatca aacccaatag aatcctgcca gaatttctac aaagatttca
      721 cattacagat cgacatggct ttcaacgtgt tcttccttct ctactttggc ttgcggttta
      781 ttgcagccaa tgataagctg tggttctggc tggaagtgaa ttctgtagta gatttcttca
      841 cagtccctcc cgtgtttgtg tctgtatact taaacagaag ttggcttggc ttgagatttt
      901 taagagctct cagactgatt cagttttcag aaattttgca gtttctgaat atccttaaaa
      961 caagtaattc catcaagctg gtgaacctgc tctccatatt tatcagcacg tggctgactg
     1021 cagctggatt catccacttg gtggagaatt caggggaccc atgggaaaat ttccaaaaca
     1081 accaggcact tacgtactgg gaatgtgtct acttactcat ggtcacaatg tctacagtgg
     1141 gttacgggga tgtttatgca aaaaccacgc ttggacgcct cttcatggtc ttcttcatcc
     1201 tcgggggact ggccatgttt gccagctacg tccctgaaat catagagtta ataggaaacc
     1261 gcaagaaata cgggggctcc tatagcgcgg ttagtggaag aaagcacatt gtagtctgtg
     1321 ggcacattac tctggagagt gtctctaact tcctgaagga ctttctgcac aaggaccggg
     1381 atgatgtcaa cgtggagatt gtttttcttc acaacatctc ccctaacctt gagcttgagg
     1441 ctctgttcaa acggcatttc actcaggtgg agttttatca gggctctgtc ctcaatccac
     1501 acgatcttgc cagagtcaag atagagtcag cagatgcatg tttgatcctt gccaataagt
     1561 actgcgctga cccggatgca gaagatgcct ccaacatcat gagagtgatc tccataaaga
     1621 actaccaccc aaagatcagg atcatcactc agatgctgca gtatcacaac aaggcccatc
     1681 tgctcaacat ccccagctgg aactggaaag agggtgatga cgcaatatgc cttgcagagc
     1741 tcaagttggg tttcatagcc cagagctgtc tggctcaagg cctctccaca atgcttgcca
     1801 atctcttctc tatgaggtca ttcataaaga ttgaggaaga tacatggcag aaatactact
     1861 tggaaggagt ctccaatgaa atgtacacag aatatctttc cagtgccttc gtgggtctgt
     1921 ccttccctac tgtttgtgag ctgtgttttg tgaagcttaa gctcctgatg atagccattg
     1981 agtacaagtc tgccaacagg gagagccgaa gccgaaagcg aatattaatt aaccctggga
     2041 accaccttaa gatccaagaa ggtactttag gatttttcat cgcaagtgat gccaaagaag
     2101 ttaaaagggc atttttttac tgcaaggcct gtcatgatga cgtcacagat cccaaaagaa
     2161 ttaaaaaatg cggctgcagg cggcttgaag atgagcaacc gccaacactg tcaccaaaaa
     2221 aaaaacaacg taatgggggc atgaggaact cacccaacac atccccgaag ctgatgaggc
     2281 atgacccctt gttaattcct ggcaacgatc agattgacaa catggactcc aatgtgaaaa
     2341 agtacgactc cactggaatg tttcactggt gcgcacccaa ggagattgag aaagtcatct
     2401 tgactcgaag tgaagctgcc atgactgtcc tgagtggtca tgttgtagtc tgcatctttg
     2461 gggatgtcag ctcagccctg attggcctcc ggaacctggt gatgccgctt cgtgctagca
     2521 attttcacta tcatgagctc aaacacattg tgtttgtggg ctccatcgag tacctcaagc
     2581 gggagtggga aacactgcac aacttcccca aagtatccat attgcctggt acgccattaa
     2641 gtcgggctga tttaagggct gtcaacatca acctctgtga catgtgcgtt atcctgtcag
     2701 ccaatcagaa taatattgat gatacttcgc ttcaggacaa ggaatgcatc ttggcgtcac
     2761 tcaacatcaa atctatgcag tttgatgaca gcatcggggt cttgcaggct aattcccaag
     2821 gattcacacc tcctggaatg gacagatcat cacctgacaa cagcccagtg cacgggatgt
     2881 tacgccagcc gtccatcaca accggagtca acattcccat catcacggaa ctcgctaagc
     2941 cgggcaagtt gcctttggta tcagtcaatc aggaaaaaaa cagtgggacg cacattctaa
     3001 tgataacgga gttggtgaac gatactaatg ttcagttttt ggaccaagac gatgatgatg
     3061 accctgacac agagctgtac ctcacacagc cctttgcttg tgggacagca tttgccgtta
     3121 gcgtcctgga ctcactcatg agcgcgacat acttcaatga caatatcctc accctaatac
     3181 ggaccctggt gacaggagga gccacgccag agctcgaagc tttaatagct gaggagaatg
     3241 cacttcgagg gggttacagc actccgcaga cactagccaa cagggaccgc tgccgagtgg
     3301 cccagttagc cctgttggat gggccctttg cggacttagg ggatggtggt tgttatggtg
     3361 atctgttctg caaagctctg aaaacatata acatgctttg ttttggaatt taccggctga
     3421 gagatgccca cctcagcacc cccagccagt gtacaaaaag gtacgtcatc accaatcccc
     3481 cctacgagtt tgaactcgta ccgacagacc tgatcttctg cctgatgcag tttgaccaca
     3541 acgctggcca atcccgggcc agtctgtctc attcctccca ctcctcacag tcgtccagta
     3601 agaagagctc ctctgtccac tccatcccgt ccacagcaaa tcggccaaac cggcccaagt
     3661 ccagggagtc ccgcgacaaa cagaacagaa aagaaatggt ttacagatga gacggataat
     3721 gcctatccca gaaacattca aatcaagccc atgagtaccc acatggctaa ccagatcaac
     3781 caatataaat ccacaagcag cctgatccct ccaatcagag aagttgaaga tgaatgttga
     3841 ttcccaggag accagaacta tttttttaaa ggctgacaaa ctgcataaat ggtgacgtga
     3901 cttctcttcc tcttacatgc tgaatcactg gtggaaacac tagaatatgc aagaattgca
     3961 agaaaataaa attagacaga tctttctctt tgccttgaat attgcttcca tgtattttgg
     4021 gaaagaaaat tatttcattt ataaatgaac atactaaaat agtcatgtat agttcctagt
     4081 gttttaaatt atttttattt attagaaaga aaatatattt ttcttttttt cattgtcaga
     4141 tatcttccca tatatcacag caatgcaaaa ttaaaatgag taagtggcca gattccttaa
     4201 tgcacatttc tcttctttct ctcttttcga ggagaatgat ggtcaccacc ccaatttgta
     4261 atgaagggaa atggattact aaaacaaatt taagatgacc aacaatgtac acacatattt
     4321 tagtaaatca aaaataaaaa gtaaaagaag tggaggtgaa tattatttcc ctctctccca
     4381 gacaggtgta tgttttgatc tgcattgaca ccagctctta caaatcagac ttcattatct
     4441 tcacagttga aagtcatctc tttgtagttt cagttttcct ccagtgtttc ttatcctttg
     4501 ttcatatatt tagaagaaag agcttcttgc aatgtttctg cagttgccag atcaggttta
     4561 agcacccagc tcaggatatg ggaggaagtg tttatcggca cacctagaat atgaaggtca
     4621 tgagccagag gcacccagga gcaggctctg ttgttctcat ggtgaggcct cctccaccat
     4681 gttttatgct ccttgtttca tacttctgag tcacccaagc aagttatgtg gactgtggga
     4741 ggatctggaa tgttctttcc cttattcaga gcttttagcc tgaaagttgg tgaacatcaa
     4801 cgcatagttt tacctaacaa taggatctaa gaagtatgca tgcttatcac aaatttttgg
     4861 tttaaaagtc agacaatgtt cactgtcaag gaaatgactt ttgttcaaat tttcatttgc
     4921 tggctttcct tggtcgaatc gctgggacaa aaacattcaa actcgctaaa atacttacga
     4981 aaacaaaacc aaccaaccaa ctaaccaaac aaacaaaaac cagtaacaac caaaaggaaa
     5041 gtaagtcatg ctcttaacat agactgttag aaaaaggcct ttgaaaatta ccagcaatga
     5101 aataaaatgt atcagccact tcaacctgtg gtcaaaagat ttttctagcc atcagatgca
     5161 atcgtgatgt ctgagctctg ctgttgcatg catgcccagt gtatagtcac ctcaacaact
     5221 gtttcaatcc aacaactcag aaagtctttt cagcttcgca gtgatcaacc tttgatatga
     5281 tcatcagggt ctctggagaa gcatgggtgt gactgtaatt tttacttcct taaaaatgta
     5341 aaatgtggac actttttcaa actaaccaaa atagccccta cagtcttatg acatctcttc
     5401 catgaactct ttaggccatg actcagtggt cctagttatg atagtataaa tcagatcaga
     5461 gcaaagcaat gtgatcacca tggaaacata tgtccactaa ggactccaat taccaatgta
     5521 agcaaaatga gcttccacct ctgtccactc cctgcagagc caatatagct gtgcttccac
     5581 cttgtaggca ttgtcctgca gtgacctgtc ggactgtggt tactctgttg tcacactttg
     5641 tagaattagt tctataaaag tatcgtgaat ataatgtgtg tccaattgga ttgttaacac
     5701 aaaaatattt ataatcaatc actagtctac tcaggctaaa atgatgtcag cagttttata
     5761 ttcgggctta taaacatttc ttggtttcca acagaactca gatgtgttgg tggggagaat
     5821 ccactgccat tggattcctg aaagcattct tgacagcatt cctgaaagct aagacttcct
     5881 ttaaaatcat gattttgcat tggggttacc taagtacatt catgtcaatc cacgctcttt
     5941 ttcccttcct gaaccacagt aggctcttct tcacactcgt tcccacctta cctctgaatt
     6001 tgtcttagtt ctttgatctc ctatggtgac caatgttgtt tacctaaata atcttccatt
     6061 agaagaataa tggtcttacc actctacttg atgcaaggca ggaacccgaa gcatttccca
     6121 ctctagctat tgatttaaat ctaaacttgt ttctcaacca caatggctct gatagtttcc
     6181 tacagtaagg ggctccctct gtttaccaag cagcttccca aacccaagat agtagaaatc
     6241 tattttcttc tacgatagag taataaagga ggaacagatc aaacatgatt ctcagattgt
     6301 atagaataga gttcccattt ggcaactcag ttgaaacaaa aataacattt gttaaaaata
     6361 atgcgaaaga aaacatgaaa aatttccagt ttgtttctgt tggttgagtt ctgagtcatc
     6421 tgtaggataa aacactgtct ctccaaccac attcacctta ggccaatggt ggtcctcttt
     6481 gaaatattcc acattagaag gctctgatct ttattccctg gttagggttc cagaaaagct
     6541 atctaggcag actaatgatg cttgggtact ttgggaatca gaccatttgt aaaacaaaaa
     6601 ctacttgtgt atgagtacat cactgccatc accatctttc tcgtggatgg agtttgtctt
     6661 tgtctatgtg actgtatttt gaaattaccc tcccctgcta taaccaatag aagacattca
     6721 tttacagtgt tttatgttta tgaattgatt atgtctaagt cacctggaag gacagacttc
     6781 cttctacaag ttacactttg tgtttgttgt tgttatttgt ttgtttgttt attaagtgtt
     6841 ggaagacttt aatcaaggaa ttagaatggt ttgggttgag ttggaagtgg agagtgggaa
     6901 aatgtatgaa attagggata ttttgaatgg aaaacaggaa gtgctcacct atgcctgatt
     6961 tagaagttca agagcactca tatataatta gctattaact attaaaattt tctttaaatc
     7021 ctccagcttc agtgacttaa acatgaagct ggtttgttga gatggtgaag gtccctttca
     7081 gaccaactaa ggcagtgctt gccttgcctc agtcaagtcc ttggatacca ctttttccag
     7141 agctgtaatt atggctcttc tggccatatt atcaatatag catttccgag ttgaacaaaa
     7201 catccactct ctggtctgga caaagaacag tcaggttggt tcctatgctc ttcagcttga
     7261 aagctcttag agtggagata caaaggttta tgtccagatt cacctccacc actaatgagc
     7321 tggttttatt cattctggtt tctctttaca gcctccctcc ccaccgtccc tgtctctttg
     7381 tgcctcaaag atttggatag tctcttcatt tctagcccac atctccaaca atatgtagag
     7441 agagtctgga caaccctact gtgctggtgt cattcccgtt catgttcccc catctacact
     7501 cacccttatt tctcattcca ttccactcgt ctgtttgtgc tcatgttagc acagtatctt
     7561 tgctgcttaa atacataagt gtcgttttga gcttcatttt cgtccaggag aacctgtgga
     7621 aagaaagata gaaagaggta gaaaagtaca aagaaaatca gtatcagata agtgacttag
     7681 agtcatcttg actaccaaga gatcacagaa attcacagaa ctgaagaatc actttcaatt
     7741 ttcccactag atttcctttc tgtccctgct gttttcccca tgttcttaac aagtattttt
     7801 cagttgtaaa acagaatgat ttctcgttta aatcttatga tttcttaata aaatgaggag
     7861 tccctttgga ctcatttagg ttacttaaac taagtttatc caaactggcc attgcccatg
     7921 catgaccacg ttattagtaa ttccaagatt accaccaaaa taaggtaatg cagaggatat
     7981 tttaaatttc atttttgaat acttatgcta agactttaaa atattaagaa agggacacat
     8041 gtggccaaag actcttgtta ggatcccctt gcattccagg ctcacatggc ttgctggaga
     8101 aaggattctc atttagcaat ttttttttga ttcttttttt tttttcggag ctggggaccg
     8161 aacccagggc cttgcgcttc ctaggcaagc gctctaccac tgagctaaat ccccaacccc
     8221 agcaattttt ttctagacaa ttctttttaa ctggattttt gagcatgaaa atgtgtaaca
     8281 cagagaatat agcaaaaggt tagaaaaaag aacaaaaaag gtagatgcaa cactttaaga
     8341 aactgtggaa ggaggtggta cctaagtaga agagaagagg ttcccttgaa cctgaatgga
     8401 aaaaatgata ggttgggctc tagagacttt ccaacaggaa atgctttgtt tgactgaagg
     8461 cacaaggatc ttgaggtttt tttttccaat gaaaggagct atggtgtact gctaccaagg
     8521 tgattttggt atgtagagca actcaccttc gataataagt ttgtttccat gcactcactg
     8581 aaaagaaaag tatggcaaga gtttatggaa gaaactcgga agtaggtagt tgaagagcca
     8641 catttaaatt ggagcattga tttagaaagt cagagagaat cactgaaagg gctgaacaaa
     8701 ggatagtgat gattgcaccc tttgggggtt gaagcctttg agatgttcag ttctttcaga
     8761 aacataggct ctcaagctga accttctctc agcagcatgg gatctcaagc aaatttcact
     8821 cagcaacact caacaacatc tcaccatgaa ggacaccaac cttcctccta catatctacc
     8881 ctgttatgca gtactcaatt attaaaatag ccatggatcc actgcataga ttaaagggaa
     8941 ggaatagatt ttcagatttt tttaaaaaag aagcttgctg gctatactat tttacagaca
     9001 tgactataat acgaatgcca agtagaccac caagaaatca tagaataaac aagaatacag
     9061 gacttccaac tatgagtgag cattatcatg ccatcttgtc aggtaatctg ctaactgggc
     9121 tttagtagac agcaccagta catgtttgtc aaaggaatgt tgggggattg aatataacac
     9181 attcttgctt gcagtaagct taaaaatcca acataaaggg gaaccctaaa gagccagtag
     9241 agaaagaaca gacatgcata taacactgtc ctctaataga atgggagatg aaagaacaag
     9301 cagtttgaag tggtttgtag actagatggg gtttcagagt taggtgagct gaggagcaac
     9361 attccagctc attcaggaat gtggatggat caatgatttc aataatttat caagtaatcc
     9421 ctgggtactt cttgctggac agctgtgtcc cttctaggca acagaagaca tgtaaaacat
     9481 acatgcaagg aagacttttt attgaatggg ctacatagca aataagtacc atgacacttt
     9541 gtggaagttc gacttgaagt ctaaggctgg ataggagttt gaaaggttct atacaaagga
     9601 agtgcttact gcagatctca aggacagagc agtgcaaagg gacacaaagg gacaggaagg
     9661 aagagaagat gagaatgttg gacaagaatt taggcagctg agaaaagggt tgttttcttc
     9721 cctgtacaaa ggagaggagg agagatgcac tcccactgtg aaggtgttct gggctgggaa
     9781 tgcaggcaga gctgagaaga tgatcttgga gttcctggtc ctaaggaagg ttcctatacc
     9841 acacacacac acacacacac acacacacac acacacacac acacacacac gaggtaatta
     9901 taaaatgaga agcctggatg acgtttttca ataaaggaga aagtataggc ctagacaaca
     9961 ggtcagtaga actgtctagg gactacagcc aagttttcag ggcctcaata gagcaagcca
    10021 agctgatgtg attaggacag aatatccatt tagtattgag agaataggaa acagagaggg
    10081 agatttagaa atagaacaaa atggggcatc caatgagggt tgagatcaag ggtatctgaa
    10141 atggtgagtc aaaagtgatg aggcagactg gaaggaccaa tttccccttt gtctttatct
    10201 catatggttg ccatgaagag tggattaaag tgggaaacta gcaggccatt ttaggttact
    10261 ttttaaactg tggaaatgga ggatatgtgg ggtatctagg tcatcagcat gccttctaac
    10321 atacccacaa aaaagtctgg agaggtggtc tctcctttta tcagttcata tcaccccccg
    10381 cccccattgt acctcatccc tcgggtatag cacattcaga accttctcgt tacgacagtc
    10441 taaatacaca aaatactaag gtcacctgat ggcttttctg gaccagagta aacacgagag
    10501 ctgttggcca gttgaaaatt aatctccttc ctaggtggaa tattaaattg aaaagagttt
    10561 tccccctggt ttgtttatcc cacagggaac ttctgtgttt cttcttgttc tccaaagctg
    10621 accccaaagc tctccagggc ctgttttagg aaacagcacc tgccctgctg tttagagtct
    10681 tagatggaaa tgtacacaca caacacatgt gcctgtcaaa gcatcccaga acttcctgcc
    10741 ctagggatcc aggaggtagt catccaggag gaggtagtca tggggaaggg agcccctaca
    10801 atgagacaag tcaagcccca ggtctacact aaaccagagc ctttctcacc ccagtacctc
    10861 tgttctcctt ttccttccgc tgtgtacaca tttgaccaga actgcaacat tcaaacataa
    10921 acccagatct gcctggaaat cctaattaaa ggcgttcagt tcccaggtgg tacgatcaca
    10981 tcctgcctaa agtgtctggc tcacatccca ccatgcagat gctgaaaaga gacttttccc
    11041 catttaacgc caaaaccttc atttggaatt tcttcagggg acaaaggggg aaaaaacaat
    11101 tcagaggtac ccatatgcag aggtcagcct acaacacgtt cattaaggga aaagagtgtg
    11161 gtttgtttat ctgtttttca cactttgatt gctggcatgg tgactatggc tatgataaat
    11221 tttgtaacca gacaggaact ggagaagaga ataaatgctg gtgctctgaa atgacctacc
    11281 caactctgtc atctgtaaca accagtgata actctcttgt cttgtaaaaa agggtttgta
    11341 cataacttgt acatggtttc attttgtatt tttcgcagat taaaaattta tgtatttgtg
    11401 ttctaaaa
//
