LOCUS       NM_017297               3335 bp    mRNA    linear   ROD 06-NOV-2024
DEFINITION  Rattus norvegicus potassium inwardly-rectifying channel, subfamily
            J, member 5 (Kcnj5), mRNA.
ACCESSION   NM_017297 XM_346827
VERSION     NM_017297.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 3335)
  AUTHORS   He,F., Zhao,J., Li,L. and Guo,R.
  TITLE     Effect of small interference RNA on the acetylcholine-sensitive
            potassium channel in H9c2 cells
  JOURNAL   Ann Clin Lab Sci 45 (1), 58-63 (2015)
   PUBMED   25696012
  REMARK    GeneRIF: The KCNJ5 gene and Kir3.4 protein were measured, and
            acetylcholine-sensitive potassium channel currents were recorded by
            patch-clamp.
REFERENCE   2  (bases 1 to 3335)
  AUTHORS   Bingen,B.O., Askar,S.F., Neshati,Z., Feola,I., Panfilov,A.V., de
            Vries,A.A. and Pijnappels,D.A.
  TITLE     Constitutively Active Acetylcholine-Dependent Potassium Current
            Increases Atrial Defibrillation Threshold by Favoring Post-Shock
            Re-Initiation
  JOURNAL   Sci Rep 5, 15187 (2015)
   PUBMED   26487066
  REMARK    GeneRIF: Blocking IK,ACh-c by tertiapin significantly decreased
            DFT, which correlated with a significant increase in wavelength
            during reentry. Genetic knockdown experiments with Kcnj5-specific
            shRNA to modulate IK,ACh-c, yielded similar results
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3335)
  AUTHORS   Liang,B., Nissen,J.D., Laursen,M., Wang,X., Skibsbye,L.,
            Hearing,M.C., Andersen,M.N., Rasmussen,H.B., Wickman,K.,
            Grunnet,M., Olesen,S.P. and Jespersen,T.
  TITLE     G-protein-coupled inward rectifier potassium current contributes to
            ventricular repolarization
  JOURNAL   Cardiovasc Res 101 (1), 175-184 (2014)
   PUBMED   24148898
  REMARK    GeneRIF: localized in intercalated discs in rat ventricle, whereas
            it was expressed in intercalated discs and outer sarcolemmas in rat
            atrium
REFERENCE   4  (bases 1 to 3335)
  AUTHORS   Kloukina,V., Herzer,S., Karlsson,N., Perez,M., Daraio,T. and
            Meister,B.
  TITLE     G-protein-gated inwardly rectifying K+ channel 4 (GIRK4)
            immunoreactivity in chemically defined neurons of the hypothalamic
            arcuate nucleus that control body weight
  JOURNAL   J Chem Neuroanat 44 (1), 14-23 (2012)
   PUBMED   22465809
  REMARK    GeneRIF: the GIRK4 channel protein plays a role in regulating
            membrane excitability in chemically defined neurons of the arcuate
            nucleus that control body weight
REFERENCE   5  (bases 1 to 3335)
  AUTHORS   Christensen,H.R., Zeng,Q., Murawsky,M.K. and Gregerson,K.A.
  TITLE     Estrogen regulation of the dopamine-activated GIRK channel in
            pituitary lactotrophs: implications for regulation of prolactin
            release during the estrous cycle
  JOURNAL   Am J Physiol Regul Integr Comp Physiol 301 (3), R746-R756 (2011)
   PUBMED   21653876
  REMARK    GeneRIF: Estradiol regulates dopamine-activataed GIRK channel
            activity in pituitary lactotrophs, regulating prolactin release
            during the estrous cycle.
REFERENCE   6  (bases 1 to 3335)
  AUTHORS   Dobrzynski,H., Marples,D.D., Musa,H., Yamanushi,T.T., Henderson,Z.,
            Takagishi,Y., Honjo,H., Kodama,I. and Boyett,M.R.
  TITLE     Distribution of the muscarinic K+ channel proteins Kir3.1 and
            Kir3.4 in the ventricle, atrium, and sinoatrial node of heart
  JOURNAL   J Histochem Cytochem 49 (10), 1221-1234 (2001)
   PUBMED   11561006
REFERENCE   7  (bases 1 to 3335)
  AUTHORS   Huang,C.L., Feng,S. and Hilgemann,D.W.
  TITLE     Direct activation of inward rectifier potassium channels by PIP2
            and its stabilization by Gbetagamma
  JOURNAL   Nature 391 (6669), 803-806 (1998)
   PUBMED   9486652
REFERENCE   8  (bases 1 to 3335)
  AUTHORS   Ferrer,J., Nichols,C.G., Makhina,E.N., Salkoff,L., Bernstein,J.,
            Gerhard,D., Wasson,J., Ramanadham,S. and Permutt,A.
  TITLE     Pancreatic islet cells express a family of inwardly rectifying K+
            channel subunits which interact to form G-protein-activated
            channels
  JOURNAL   J Biol Chem 270 (44), 26086-26091 (1995)
   PUBMED   7592809
REFERENCE   9  (bases 1 to 3335)
  AUTHORS   Krapivinsky,G., Gordon,E.A., Wickman,K., Velimirovic,B.,
            Krapivinsky,L. and Clapham,D.E.
  TITLE     The G-protein-gated atrial K+ channel IKACh is a heteromultimer of
            two inwardly rectifying K(+)-channel proteins
  JOURNAL   Nature 374 (6518), 135-141 (1995)
   PUBMED   7877685
REFERENCE   10 (bases 1 to 3335)
  AUTHORS   Iizuka,M., Kubo,Y., Tsunenari,I., Pan,C.X., Akiba,I. and Kono,T.
  TITLE     Functional characterization and localization of a cardiac-type
            inwardly rectifying K+ channel
  JOURNAL   Recept Channels 3 (4), 299-315 (1995)
   PUBMED   8834003
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from BC087022.1.
            
            On Dec 26, 2004 this sequence version replaced NM_017297.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC087022.1, D50135.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN13663600, SAMN13663601
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..3335
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10116"
                     /chromosome="8"
                     /map="8q21"
     gene            1..3335
                     /gene="Kcnj5"
                     /note="potassium inwardly-rectifying channel, subfamily J,
                     member 5"
                     /db_xref="GeneID:29713"
                     /db_xref="RGD:61971"
     exon            1..174
                     /gene="Kcnj5"
                     /inference="alignment:Splign:2.1.0"
     exon            175..1113
                     /gene="Kcnj5"
                     /inference="alignment:Splign:2.1.0"
     CDS             177..1436
                     /gene="Kcnj5"
                     /note="CIR; GIRK4; KATP-1; heart KATP channel; cardiac
                     inward rectifier; inward rectifier K(+) channel Kir3.4;
                     potassium voltage-gated channel subfamily J member 5;
                     potassium channel, inwardly rectifying subfamily J member
                     5; GIRK-4"
                     /codon_start=1
                     /product="G protein-activated inward rectifier potassium
                     channel 4"
                     /protein_id="NP_058993.1"
                     /db_xref="GeneID:29713"
                     /db_xref="RGD:61971"
                     /translation="MAGDSRNAMNQDMEIGVTSQDHKKIPKQARDYIPIATDRTRLLP
                     EGKKPRQRYMEKTGKCNVHHGNVQETYRYLSDLFTTLVDLKWRFNLLVFTMVYTITWL
                     FFGFIWWLIAYVRGDLDHVGDQEWIPCVENLSGFVSAFLFSIETETTIGYGFRVITEK
                     CPEGIILLLVQAILGSIVNAFMVGCMFVKISQPKKRAETLMFSNNAVISMRDEKLCLM
                     FRVGDLRNSHIVEASIRAKLIKSRQTKEGEFIPLNQTDINVGFDTGDDRLFLVSPLII
                     SHEINEKSPFWEMSRAQLEQEEFEVVVILEGMVEATGMTCQARSSYMDTEVLWGHRFT
                     PVLTLEKGFYEVDYNTFHDTYETNTPSCCAKELAEMKRNGQLLQSLPSPPLLGGCAEA
                     EKEAEAEHDEEEEPNGLSVSRATRGSM"
     exon            1114..3311
                     /gene="Kcnj5"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 cacgcctctg ctagctggac ctgcgacacc agagtggctc agggcggctg tcggtacgta
       61 ggcaatgcct gaggctatgg aatcccctgg agagccctga gagccacacg caccgccttg
      121 aagggatcgc cagaagttag ccccaagggt tcagagaagc tctctgctct acgactatgg
      181 ccggtgattc taggaatgct atgaatcaag acatggagat aggagtcacc tcccaggacc
      241 acaagaagat ccccaaacag gctcgggatt acatccccat tgccacagac cgcactcgcc
      301 tgctgccgga aggcaagaag ccacgccagc gctacatgga gaagaccggc aagtgtaacg
      361 tgcaccatgg caatgttcag gaaacctacc gctacctaag tgacctcttc accaccctgg
      421 tggacctcaa atggcgcttc aaccttctgg tcttcaccat ggtctacacc attacttggc
      481 tattctttgg cttcatctgg tggctcattg cttatgtccg aggtgatctg gaccacgtgg
      541 gtgaccaaga gtggatccct tgtgttgaaa accttagtgg ctttgtgtct gctttcctgt
      601 tctccattga gacagaaaca accattgggt atggcttcag agtcattaca gagaagtgtc
      661 cagaggggat cattctcctt ctagtgcagg ccatcctggg ctctattgtt aatgccttca
      721 tggtgggttg catgtttgta aagatcagcc agccaaagaa gagagcagag accctcatgt
      781 tctccaacaa tgctgtcatc tccatgcggg atgagaagct gtgcctcatg ttccgggtag
      841 gggacctccg aaactcccat atcgtggagg cctccatccg cgccaagctt atcaagtccc
      901 ggcagaccaa agaaggggaa ttcatcccct tgaaccagac cgacattaac gtgggctttg
      961 acactggtga cgaccgcctc ttcctggtgt cccccctcat catctcccat gagatcaatg
     1021 agaagagccc tttctgggag atgtctcgtg ctcaactgga gcaggaagag ttcgaggttg
     1081 tggtcatact agaagggatg gtagaagcca caggcatgac ttgccaagca cggagctctt
     1141 acatggatac agaggtgctc tggggtcacc gattcacacc agtcctcacc ttggaaaagg
     1201 gcttctatga ggtggactac aacactttcc acgacaccta tgagaccaac acacccagct
     1261 gctgtgccaa ggagctggca gaaatgaaga ggaatggtca gctcctccag tccttgccca
     1321 gtcctccttt gcttgggggc tgcgctgagg ctgagaaaga agcagaggct gagcacgatg
     1381 aggaggagga acccaatgga ctgagtgtgt cccgggcaac aaggggctca atgtgaagcc
     1441 tgtgtgctac ccagagcctc ttcctgtcag acaccaagga gcctgagagg ccagagaggc
     1501 caattagttg agcaagcttt gtggagtccc aggagtatgg gacttctaca aagaggaaca
     1561 gtgtggtgag gcctcacatc tcctcctcag ggcctcggag caagtggcgt tgactaggcc
     1621 tcccaagaag tgttttctcg gcctctgtga aggagagcat taggggtcac cagggtgggc
     1681 ttcccaagga ggagttctaa ggtgtcatcc atcctctctc tgactgctga agcctgctca
     1741 ctggcctccc tgattctctg tttcctcagc acaatcactg aggcctgtga atggagggac
     1801 taattccatc catggatact caggcctcca actggggatg agttcacaga ggccttccag
     1861 gggcaatgcc ctgcagtctg aggccaaaac ccactgatct aatagcttct tagcaaggcc
     1921 ccttaacctc tgatactacc tactgtgtgt tttcctccat ggatactcag gcctccaact
     1981 ggggatgagt tcacagaggc cttccagggg caatgccctg cagtctgagg ccaaaaccca
     2041 ctgatttaat agcttcttag caaggcccct taacctctga tactacctac tgtgtgtttt
     2101 cctcttctgt gtcctcttcc atggacacta cggcttacag ggtaattgat ttaattagag
     2161 tggccctagg acactgagta cctaggagca cacacaatgt cgtcctcaca actgcctggc
     2221 actcctactc acacttcaaa ggacagacac agcagttgtg gcttttgtag gagtgtgtgg
     2281 caaaagaact tcaaagaacc gacctctaaa gcggtcccgg ggaacatgtg aatttcagag
     2341 agccagatta gctcattggt actaacatag ggatggcaga caggaaagag acacaaggat
     2401 aattttaaaa taagtatctg gagcctggtg ttgtgatgca tgcctgtaat cctagcattt
     2461 ggcaggctga ggcagaattg agaatttgaa gccagccggg gctatatagc cctgtctgaa
     2521 aaaagcaaga gacaaactac aaccaaggaa acatctgcca cagttgttgc atgggggctc
     2581 ctcggagaaa agtaggctat gaaacacaag tgacaagagg ccataaaggc atcttcagtg
     2641 ccaagccaag tgtggaccag agtcttgggc tgccgctgag atgcctctgg gcctcccccc
     2701 tatggaacag ctccaacatg gtggtgggac ctagagggag gtccctgaga acaggatgag
     2761 agttggtggg tgactaggga aagaagagtc taagtgtgga gcagagcctt ggggtccaca
     2821 gcctcagaga cctttctctt ccagtctagg agatctgatg gtcagtcagt gaagcattat
     2881 gttgaaaaca aagttggcct ggtcttggga atatgctagc atgatcccca acacctctac
     2941 ccgggtgttc atggctctca accagcctct gagaatagaa gcaccttcct gccaggccct
     3001 agagaacttt ctctggcatc tccaaggaga tgagacgcta aggtaggcta gggaacagca
     3061 tcctaacctc aggccttgtt ccccttagac agggtgccca ggtctctagg ctggtgagaa
     3121 ggtgtcctag gatggctagc tacattgtgg accagacaca gatagaagag gggaaccttg
     3181 acttgttatc tgggctgaat cttgaaagac agtctaaagc cagtggttct caaccttcct
     3241 aatgctgtga ccttttaata tagttcctca tgttgtgatg accccaatca taaaattatt
     3301 tttgttccta aaaaaaaaaa aaaaaaaaaa aaaaa
//
