LOCUS       NM_001006967            2175 bp    mRNA    linear   ROD 31-MAR-2024
DEFINITION  Rattus norvegicus lysyl-tRNA synthetase 1 (Kars1), transcript
            variant 1, mRNA.
ACCESSION   NM_001006967 XM_214694
VERSION     NM_001006967.2
KEYWORDS    RefSeq.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 2175)
  AUTHORS   Park,B.S., Jo,H.W. and Jung,J.
  TITLE     Expression profile of aminoacyl-tRNA synthetases in dorsal root
            ganglion neurons after peripheral nerve injury
  JOURNAL   J Mol Histol 46 (1), 115-122 (2015)
   PUBMED   25467976
REFERENCE   2  (bases 1 to 2175)
  AUTHORS   Kim,D.G., Lee,J.Y., Kwon,N.H., Fang,P., Zhang,Q., Wang,J.,
            Young,N.L., Guo,M., Cho,H.Y., Mushtaq,A.U., Jeon,Y.H., Choi,J.W.,
            Han,J.M., Kang,H.W., Joo,J.E., Hur,Y., Kang,W., Yang,H., Nam,D.H.,
            Lee,M.S., Lee,J.W., Kim,E.S., Moon,A., Kim,K., Kim,D., Kang,E.J.,
            Moon,Y., Rhee,K.H., Han,B.W., Yang,J.S., Han,G., Yang,W.S., Lee,C.,
            Wang,M.W. and Kim,S.
  TITLE     Chemical inhibition of prometastatic lysyl-tRNA synthetase-laminin
            receptor interaction
  JOURNAL   Nat Chem Biol 10 (1), 29-34 (2014)
   PUBMED   24212136
REFERENCE   3  (bases 1 to 2175)
  AUTHORS   Ofir-Birin,Y., Fang,P., Bennett,S.P., Zhang,H.M., Wang,J.,
            Rachmin,I., Shapiro,R., Song,J., Dagan,A., Pozo,J., Kim,S.,
            Marshall,A.G., Schimmel,P., Yang,X.L., Nechushtan,H., Razin,E. and
            Guo,M.
  TITLE     Structural switch of lysyl-tRNA synthetase between translation and
            transcription
  JOURNAL   Mol Cell 49 (1), 30-42 (2013)
   PUBMED   23159739
REFERENCE   4  (bases 1 to 2175)
  AUTHORS   Fang,P., Zhang,H.M., Shapiro,R., Marshall,A.G., Schimmel,P.,
            Yang,X.L. and Guo,M.
  TITLE     Structural context for mobilization of a human tRNA synthetase from
            its cytoplasmic complex
  JOURNAL   Proc Natl Acad Sci U S A 108 (20), 8239-8244 (2011)
   PUBMED   21536907
REFERENCE   5  (bases 1 to 2175)
  AUTHORS   Yannay-Cohen,N., Carmi-Levy,I., Kay,G., Yang,C.M., Han,J.M.,
            Kemeny,D.M., Kim,S., Nechushtan,H. and Razin,E.
  TITLE     LysRS serves as a key signaling molecule in the immune response by
            regulating gene expression
  JOURNAL   Mol Cell 34 (5), 603-611 (2009)
   PUBMED   19524539
REFERENCE   6  (bases 1 to 2175)
  AUTHORS   Wahab,S.Z. and Yang,D.C.
  TITLE     Synthesis of diadenosine 5',5''' -P1,P4-tetraphosphate by
            lysyl-tRNA synthetase and a multienzyme complex of aminoacyl-tRNA
            synthetases from rat liver
  JOURNAL   J Biol Chem 260 (9), 5286-5289 (1985)
   PUBMED   3988754
REFERENCE   7  (bases 1 to 2175)
  AUTHORS   Verkhogliad,I.N., Vinogradova,R.P. and Kucherenko,N.E.
  TITLE     [Study of the kinetics of thermoinactivation of
            lysyl-tRNA-synthetase from the liver of x-irradiated rats]
  JOURNAL   Radiobiologiia 25 (2), 185-190 (1985)
   PUBMED   4001316
REFERENCE   8  (bases 1 to 2175)
  AUTHORS   Hilderman,R.H., Zimmerman,J.K., Dang,C.V. and Grothusen,J.R.
  TITLE     Characterization of a homogeneous arginyl- and lysyl-tRNA
            synthetase complex isolated from rat liver. Kinetic mechanism for
            lysyl-tRNA synthetase
  JOURNAL   J Biol Chem 258 (22), 13592-13596 (1983)
   PUBMED   6315704
REFERENCE   9  (bases 1 to 2175)
  AUTHORS   Hilderman,R.H.
  TITLE     Characterization of a homogeneous complex of arginyl- and
            lysyl-tRNA synthetase: zinc and adenosine 5'-phosphate dependent
            synthesis of diadenosine 5',5'''-P1,P4-tetraphosphate
  JOURNAL   Biochemistry 22 (19), 4353-4357 (1983)
   PUBMED   6626505
REFERENCE   10 (bases 1 to 2175)
  AUTHORS   Van Dang,C., Glinski,R.L., Gainey,P.C. and Hilderman,R.H.
  TITLE     Physical and biochemical characterization of a purified
            arginyl-tRNA synthetase-lysyl-tRNA-synthetase complex from rat
            liver
  JOURNAL   Biochemistry 21 (8), 1959-1966 (1982)
   PUBMED   7082655
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000019.1.
            
            On Jan 4, 2022 this sequence version replaced NM_001006967.1.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC083652.1, SRR26360172.1878280.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA5760383,
                                           SAMEA5760386 [ECO:0006172]
            ##Evidence-Data-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-89                JAXUCZ010000019.1  56885995-56886083   c
            90-269              JAXUCZ010000019.1  56883177-56883356   c
            270-432             JAXUCZ010000019.1  56879610-56879772   c
            433-598             JAXUCZ010000019.1  56877273-56877438   c
            599-692             JAXUCZ010000019.1  56876468-56876561   c
            693-879             JAXUCZ010000019.1  56875797-56875983   c
            880-1005            JAXUCZ010000019.1  56875596-56875721   c
            1006-1125           JAXUCZ010000019.1  56874120-56874239   c
            1126-1288           JAXUCZ010000019.1  56872778-56872940   c
            1289-1462           JAXUCZ010000019.1  56872494-56872667   c
            1463-1548           JAXUCZ010000019.1  56872262-56872347   c
            1549-1634           JAXUCZ010000019.1  56871348-56871433   c
            1635-1761           JAXUCZ010000019.1  56870477-56870603   c
            1762-1905           JAXUCZ010000019.1  56868854-56868997   c
            1906-2175           JAXUCZ010000019.1  56867096-56867365   c
FEATURES             Location/Qualifiers
     source          1..2175
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="19"
                     /map="19q12"
     gene            1..2175
                     /gene="Kars1"
                     /gene_synonym="Kars; LysRS"
                     /note="lysyl-tRNA synthetase 1"
                     /db_xref="GeneID:292028"
                     /db_xref="RGD:1359653"
     exon            1..89
                     /gene="Kars1"
                     /gene_synonym="Kars; LysRS"
                     /inference="alignment:Splign:2.1.0"
     exon            90..269
                     /gene="Kars1"
                     /gene_synonym="Kars; LysRS"
                     /inference="alignment:Splign:2.1.0"
     CDS             124..2004
                     /gene="Kars1"
                     /gene_synonym="Kars; LysRS"
                     /EC_number="6.1.1.6"
                     /note="isoform 1 is encoded by transcript variant 1;
                     lysine--tRNA ligase"
                     /codon_start=1
                     /product="lysine--tRNA ligase isoform 1"
                     /protein_id="NP_001006968.1"
                     /db_xref="GeneID:292028"
                     /db_xref="RGD:1359653"
                     /translation="MLMQAAVRLVRGSLRQTSWAEWGQRELRLGQLAPFTTLRKDKPL
                     SDRRSELKRRLKAEKKLAEKEAKQKELSEKQLNQTTAAAATNHTADNGVGAEEETLDP
                     NQYYKIRSQAVQQLKVSGEDPYPHKFHVDISLTQFIQEYSHLQPGDHLTDITLKVAGR
                     IHAKRASGGKLIFYDLRGEGVKLQVMANSRNYKSEEEFVHINNKLRRGDIIGVEGNPG
                     KTKKGELSIVPREMTLLSPCLHMLPHLHFGLKDKETRYRQRYLDLILNDFVRQKFIIR
                     SKIITYIRSFLDELGFLEIETPMMNIIPGGAVAKPFITYHNELDMNLYMRIAPELYHK
                     MLVVGGIDRVYEIGRQFRNEGIDLTHNPEFTTCEFYMAYADYHDLMEITEKMLSGMVR
                     SITGSYKITYHPDGPEGQAYEIDFTPPFRRISMVEELEKVLGVKLPETSLFETEETRK
                     ILDDICVARAVECPPPRTTARLLDKLVGEFLEVTCISPTFICDHPQIMSPLAKWHRCK
                     EGLTERFELFVMKKEICNAYTELNDPVRQRQLFEEQAKAKAAGDDEAMFIDENFCTAL
                     EYGLPPTAGWGMGIDRVTMFLTDSNNIKEVLLFPAMKPEDKKETATATETPESTEASP
                     SV"
     exon            270..432
                     /gene="Kars1"
                     /gene_synonym="Kars; LysRS"
                     /inference="alignment:Splign:2.1.0"
     exon            433..598
                     /gene="Kars1"
                     /gene_synonym="Kars; LysRS"
                     /inference="alignment:Splign:2.1.0"
     exon            599..692
                     /gene="Kars1"
                     /gene_synonym="Kars; LysRS"
                     /inference="alignment:Splign:2.1.0"
     exon            693..879
                     /gene="Kars1"
                     /gene_synonym="Kars; LysRS"
                     /inference="alignment:Splign:2.1.0"
     exon            880..1005
                     /gene="Kars1"
                     /gene_synonym="Kars; LysRS"
                     /inference="alignment:Splign:2.1.0"
     exon            1006..1125
                     /gene="Kars1"
                     /gene_synonym="Kars; LysRS"
                     /inference="alignment:Splign:2.1.0"
     exon            1126..1288
                     /gene="Kars1"
                     /gene_synonym="Kars; LysRS"
                     /inference="alignment:Splign:2.1.0"
     exon            1289..1462
                     /gene="Kars1"
                     /gene_synonym="Kars; LysRS"
                     /inference="alignment:Splign:2.1.0"
     exon            1463..1548
                     /gene="Kars1"
                     /gene_synonym="Kars; LysRS"
                     /inference="alignment:Splign:2.1.0"
     exon            1549..1634
                     /gene="Kars1"
                     /gene_synonym="Kars; LysRS"
                     /inference="alignment:Splign:2.1.0"
     exon            1635..1761
                     /gene="Kars1"
                     /gene_synonym="Kars; LysRS"
                     /inference="alignment:Splign:2.1.0"
     exon            1762..1905
                     /gene="Kars1"
                     /gene_synonym="Kars; LysRS"
                     /inference="alignment:Splign:2.1.0"
     exon            1906..2175
                     /gene="Kars1"
                     /gene_synonym="Kars; LysRS"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 ttttttttct gggtccggcc agtgggcaag atggccacgc tgcgggaggg tgaagtcaaa
       61 ttggatggcg agccgaaact aagcaagaag tggtggtaat cattagttcc agggtgttct
      121 gccatgttga tgcaagctgc tgtcaggctt gttagggggt ccctgcgcca aacctcatgg
      181 gcagaatggg gtcagaggga acttcgactg ggccaacttg ctcctttcac aacgctccgc
      241 aaggacaagc cactttctga tagaagaagt gagctgaaga ggcgtctgaa agctgagaag
      301 aagctggcag agaaagaggc caagcagaaa gagctcagtg aaaagcagct aaaccagacg
      361 actgctgctg ctgccaccaa ccatactgct gacaatggtg tgggtgctga agaggagacc
      421 ctggacccaa accaatacta caagatccgt agtcaagctg tccagcagct gaaggtcagt
      481 ggggaggacc cgtacccaca caagttccat gtggacatct cactcactca gttcatccaa
      541 gaatacagtc acctgcagcc tggggaccac ctgactgaca tcaccttaaa agtagcaggt
      601 cgcatccacg ccaagagagc ctctggggga aagctcatct tctatgacct tcgaggagag
      661 ggggtcaagt tacaagtcat ggccaactcc aggaattata aatcagagga agaattcgtt
      721 cacatcaata acaaactgcg caggggagac ataatcggag tcgagggcaa tcccggaaaa
      781 accaagaagg gtgagctgag catcgtcccc cgggagatga cactcctgtc cccctgcttg
      841 cacatgctgc ctcatcttca cttcggcctc aaggacaagg aaacaaggta tcgtcagaga
      901 tacttggact tgatactgaa cgactttgtg aggcagaagt ttatcatccg ctctaagatc
      961 atcacatata taagaagttt cttggacgag ctgggattcc tagagatcga gactcccatg
     1021 atgaacatca tccccggggg agctgtggcc aagcctttca tcacctacca caacgagctg
     1081 gacatgaacc tgtacatgag aattgctcca gagctctacc acaagatgct ggtggttggt
     1141 ggcattgacc gggtttatga aattgggcgc cagttccgga acgaagggat tgatttgact
     1201 cacaatcctg agttcaccac ctgtgagttc tacatggcct atgcagacta tcatgacctc
     1261 atggaaatca cagagaagat gctgtcaggg atggtgagaa gcatcacagg cagttacaag
     1321 atcacctatc acccagatgg gccagaaggc caagcctatg agattgactt caccccaccc
     1381 ttccgaagaa tcagcatggt agaagagctt gagaaggttc tgggcgtgaa gctgccagaa
     1441 accagtctct ttgagaccga agaaactcgc aaaattctcg atgatatttg tgttgcaagg
     1501 gctgttgaat gccccccacc tcggaccaca gccaggctcc ttgataagct tgtgggagag
     1561 ttcctcgaag tgacatgcat cagccctaca ttcatctgtg atcacccaca gatcatgagt
     1621 cctctggcca aatggcaccg ttgcaaagag ggtctaactg agcgcttcga gctgtttgtc
     1681 atgaagaagg aaatatgcaa tgcgtacact gagctgaacg accccgtgcg gcagagacaa
     1741 ctgtttgaag aacaggccaa ggccaaggct gctggtgacg atgaggccat gttcatagat
     1801 gagaacttct gtactgccct ggagtacggg ctgcccccaa cagctggctg gggcatgggt
     1861 atcgaccgag tcaccatgtt tctcacagac tccaacaata tcaaggaagt acttctcttt
     1921 cctgccatga agcctgaaga caagaaggaa actgcaacag ccaccgagac cccagaaagc
     1981 acagaggcca gcccctctgt ctagaagact atgcaactat ggcctttgcg cttgagcagc
     2041 agatctggtc tgagggaggg aggggttcct gtgtggtgcc cgcccactgg acactgcagt
     2101 ccaaccacca caagagagaa gaattaaaga atttcttttt attccttgtt accaaataaa
     2161 ccatttctct cttta
//
