LOCUS       NM_001017514            1503 bp    mRNA    linear   ROD 06-NOV-2024
DEFINITION  Rattus norvegicus izumo sperm-oocyte fusion 1 (Izumo1), mRNA.
ACCESSION   NM_001017514 XM_574449
VERSION     NM_001017514.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 1503)
  AUTHORS   Lai,X., Liu,R., Li,M., Fan,Y., Li,H., Han,G., Guo,R., Ma,H., Su,H.
            and Xing,W.
  TITLE     Participation of WD repeat-containing protein 54 (WDR54) in rat
            sperm-oocyte fusion through interaction with both IZUMO1 and JUNO
  JOURNAL   Theriogenology 214, 286-297 (2024)
   PUBMED   37951137
  REMARK    GeneRIF: Participation of WD repeat-containing protein 54 (WDR54)
            in rat sperm-oocyte fusion through interaction with both IZUMO1 and
            JUNO.
REFERENCE   2  (bases 1 to 1503)
  AUTHORS   Matsumura,T., Noda,T., Satouh,Y., Morohoshi,A., Yuri,S., Ogawa,M.,
            Lu,Y., Isotani,A. and Ikawa,M.
  TITLE     Sperm IZUMO1 Is Required for Binding Preceding Fusion With Oolemma
            in Mice and Rats
  JOURNAL   Front Cell Dev Biol 9, 810118 (2022)
   PUBMED   35096839
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 1503)
  AUTHORS   Young,S.A., Miyata,H., Satouh,Y., Muto,M., Larsen,M.R.,
            Aitken,R.J., Baker,M.A. and Ikawa,M.
  TITLE     CRISPR/Cas9-mediated mutation revealed cytoplasmic tail is
            dispensable for IZUMO1 function and male fertility
  JOURNAL   Reproduction 152 (6), 665-672 (2016)
   PUBMED   27624483
REFERENCE   4  (bases 1 to 1503)
  AUTHORS   Ohto,U., Ishida,H., Krayukhina,E., Uchiyama,S., Inoue,N. and
            Shimizu,T.
  TITLE     Structure of IZUMO1-JUNO reveals sperm-oocyte recognition during
            mammalian fertilization
  JOURNAL   Nature 534 (7608), 566-569 (2016)
   PUBMED   27309808
REFERENCE   5  (bases 1 to 1503)
  AUTHORS   Bianchi,E., Doe,B., Goulding,D. and Wright,G.J.
  TITLE     Juno is the egg Izumo receptor and is essential for mammalian
            fertilization
  JOURNAL   Nature 508 (7497), 483-487 (2014)
   PUBMED   24739963
REFERENCE   6  (bases 1 to 1503)
  AUTHORS   Fujihara,Y., Murakami,M., Inoue,N., Satouh,Y., Kaseda,K., Ikawa,M.
            and Okabe,M.
  TITLE     Sperm equatorial segment protein 1, SPESP1, is required for fully
            fertile sperm in mouse
  JOURNAL   J Cell Sci 123 (Pt 9), 1531-1536 (2010)
   PUBMED   20375058
REFERENCE   7  (bases 1 to 1503)
  AUTHORS   Inoue,N., Kasahara,T., Ikawa,M. and Okabe,M.
  TITLE     Identification and disruption of sperm-specific angiotensin
            converting enzyme-3 (ACE3) in mouse
  JOURNAL   PLoS One 5 (4), e10301 (2010)
   PUBMED   20421979
  REMARK    Publication Status: Online-Only
REFERENCE   8  (bases 1 to 1503)
  AUTHORS   Ellerman,D.A., Pei,J., Gupta,S., Snell,W.J., Myles,D. and
            Primakoff,P.
  TITLE     Izumo is part of a multiprotein family whose members form large
            complexes on mammalian sperm
  JOURNAL   Mol Reprod Dev 76 (12), 1188-1199 (2009)
   PUBMED   19658160
REFERENCE   9  (bases 1 to 1503)
  AUTHORS   Miranda,P.V., Allaire,A., Sosnik,J. and Visconti,P.E.
  TITLE     Localization of low-density detergent-resistant membrane proteins
            in intact and acrosome-reacted mouse sperm
  JOURNAL   Biol Reprod 80 (5), 897-904 (2009)
   PUBMED   19144954
REFERENCE   10 (bases 1 to 1503)
  AUTHORS   Inoue,N., Ikawa,M., Isotani,A. and Okabe,M.
  TITLE     The immunoglobulin superfamily protein Izumo is required for sperm
            to fuse with eggs
  JOURNAL   Nature 434 (7030), 234-238 (2005)
   PUBMED   15759005
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from BC079244.1.
            
            On Apr 29, 2005 this sequence version replaced XM_574449.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC079244.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760383, SAMEA5760389
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on expression, longest protein
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..1503
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10116"
                     /chromosome="1"
                     /map="1q22"
     gene            1..1503
                     /gene="Izumo1"
                     /note="izumo sperm-oocyte fusion 1"
                     /db_xref="GeneID:499152"
                     /db_xref="RGD:1565454"
     exon            1..222
                     /gene="Izumo1"
                     /inference="alignment:Splign:2.1.0"
     exon            223..535
                     /gene="Izumo1"
                     /inference="alignment:Splign:2.1.0"
     CDS             301..1452
                     /gene="Izumo1"
                     /note="izumo sperm-egg fusion protein 1; OBF; oocyte
                     binding/fusion factor; sperm-specific protein izumo; izumo
                     sperm-egg fusion 1"
                     /codon_start=1
                     /product="izumo sperm-egg fusion protein 1 precursor"
                     /protein_id="NP_001017514.1"
                     /db_xref="GeneID:499152"
                     /db_xref="RGD:1565454"
                     /translation="MGLHFTLLLAALANCLCPARLCIICDPFVVAAIKTLEQNYLPTH
                     LAPEHHEDVMKRVEQEVRNFADLPLNQNTFLGVVDEDTLEQASWSFLKDLKRITDSDV
                     KGELFVKELFWMLRLQKDIFATLVARFQKEVYCPNQCGTMSQTLIWCNKCEKQMHFCR
                     KSMDCGERQIEVHRLEDMVLDCQLSWHHASEGLTDYSFYRVWGNSSETLMSKGKEPYL
                     TKTMVGPEDAGNYRCELDTVNAGPATIIYYHVTVLPPRSVEEKPPPNIVTQEEEETPV
                     QVIVPTLEPEPEPEPIPTVTHRPEKKLKSRLLILLILGFVVLVASVIASVLHFRKTRV
                     KSKNSNVENKTSAAEFKSEAESPQKMGSRKLSQAEFHTDSSDKVEEADN"
     exon            536..610
                     /gene="Izumo1"
                     /inference="alignment:Splign:2.1.0"
     exon            611..697
                     /gene="Izumo1"
                     /inference="alignment:Splign:2.1.0"
     exon            698..718
                     /gene="Izumo1"
                     /inference="alignment:Splign:2.1.0"
     exon            719..799
                     /gene="Izumo1"
                     /inference="alignment:Splign:2.1.0"
     exon            800..900
                     /gene="Izumo1"
                     /inference="alignment:Splign:2.1.0"
     exon            901..1054
                     /gene="Izumo1"
                     /inference="alignment:Splign:2.1.0"
     exon            1055..1274
                     /gene="Izumo1"
                     /inference="alignment:Splign:2.1.0"
     exon            1275..1478
                     /gene="Izumo1"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 attggggtcc cacatgtggg atgtggcccg agtgttttgt gaagagggga cgacaaatga
       61 ctcttggagt cagtagaaaa acactaattt cctctcccag tctcccagga acaactctta
      121 gtcgtgagaa agtattttaa aaagtcctca gaacctgagc tgggcgaagc agcgcttgaa
      181 ggctcggagt tggagaacag ctattaaagg gtgttgttta agcaccctcc tccctgccct
      241 tggtttgggg tgagagaatc ctcctccggc ggtgctggca agctgtgcgt tctggcctcc
      301 atggggctac attttacact cttgctggca gctcttgcca actgcctgtg tccagcaagg
      361 ctctgcatca tatgtgaccc gtttgtggtg gctgcaataa agactttgga gcagaattac
      421 ctgcctaccc acctggcgcc cgagcatcac gaagatgtaa tgaagagggt agagcaggaa
      481 gtgaggaact tcgctgatct gcccttgaat cagaatacct ttctgggggt tgtagatgag
      541 gacacactgg aacaagcatc ctggagtttt ctgaaggatc tgaaacgtat cacggacagt
      601 gacgtaaaag gagagctctt tgtaaaggag ctattttgga tgcttcgttt gcaaaaggac
      661 atctttgcca cccttgttgc acgtttccaa aaggaagttt attgtcctaa ccaatgtggc
      721 acgatgtcgc agactctgat ctggtgtaat aagtgtgaga agcagatgca cttctgtcgg
      781 aaatccatgg attgtggaga gcgccagata gaggtgcatc gcttggaaga catggtcttg
      841 gactgccagc tcagttggca tcacgcttct gagggactta cggattacag tttctacagg
      901 gtttggggga acagttctga gaccttgatg tccaagggaa aagaacctta cctgaccaag
      961 acgatggtgg gtccagagga tgctggcaac taccgctgtg agctggacac cgtcaacgct
     1021 ggtcccgcca ccatcatcta ctaccatgtc acagtattgc ccccaaggtc tgtagaggaa
     1081 aaaccaccgc cgaacatcgt aacccaggag gaggaagaga cccctgtcca ggtgattgta
     1141 ccgacgctgg agccagagcc ggagccagag cccataccta cagttaccca tcgtccagaa
     1201 aaaaaattga aaagccgcct cttaatactg ttgatccttg gcttcgtggt tcttgtggcc
     1261 agcgtcattg cctcggtact tcactttaga aaaaccagag ttaaatcgaa gaactcaaat
     1321 gtggaaaaca aaacctctgc ggcagaattc aagtcagagg cggaatcacc ccaaaagatg
     1381 ggatcgagga agctctctca ggcagagttt cacactgact cttcggataa ggttgaggaa
     1441 gcagataact aataaagatt tgatgtgttg tctcagtaaa aaaaaaaaaa aaaaaaaaaa
     1501 aaa
//
