LOCUS       NM_001017454            2261 bp    mRNA    linear   ROD 31-MAR-2024
DEFINITION  Rattus norvegicus IST1 factor associated with ESCRT-III (Ist1),
            mRNA.
ACCESSION   NM_001017454 XM_226448
VERSION     NM_001017454.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 2261)
  AUTHORS   Gu,M., LaJoie,D., Chen,O.S., von Appen,A., Ladinsky,M.S.,
            Redd,M.J., Nikolova,L., Bjorkman,P.J., Sundquist,W.I., Ullman,K.S.
            and Frost,A.
  TITLE     LEM2 recruits CHMP7 for ESCRT-mediated nuclear envelope closure in
            fission yeast and human cells
  JOURNAL   Proc Natl Acad Sci U S A 114 (11), E2166-E2175 (2017)
   PUBMED   28242692
REFERENCE   2  (bases 1 to 2261)
  AUTHORS   Guo,Z., Neilson,L.J., Zhong,H., Murray,P.S., Zanivan,S. and
            Zaidel-Bar,R.
  TITLE     E-cadherin interactome complexity and robustness resolved by
            quantitative proteomics
  JOURNAL   Sci Signal 7 (354), rs7 (2014)
   PUBMED   25468996
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2261)
  AUTHORS   Lee,S., Chang,J., Renvoise,B., Tipirneni,A., Yang,S. and
            Blackstone,C.
  TITLE     MITD1 is recruited to midbodies by ESCRT-III and participates in
            cytokinesis
  JOURNAL   Mol Biol Cell 23 (22), 4347-4361 (2012)
   PUBMED   23015756
REFERENCE   4  (bases 1 to 2261)
  AUTHORS   Renvoise,B., Stadler,J., Singh,R., Bakowska,J.C. and Blackstone,C.
  TITLE     Spg20-/- mice reveal multimodal functions for Troyer syndrome
            protein spartin in lipid droplet maintenance, cytokinesis and BMP
            signaling
  JOURNAL   Hum Mol Genet 21 (16), 3604-3618 (2012)
   PUBMED   22619377
REFERENCE   5  (bases 1 to 2261)
  AUTHORS   Li,Y., Zhang,Y., Qiu,F. and Qiu,Z.
  TITLE     Proteomic identification of exosomal LRG1: a potential urinary
            biomarker for detecting NSCLC
  JOURNAL   Electrophoresis 32 (15), 1976-1983 (2011)
   PUBMED   21557262
REFERENCE   6  (bases 1 to 2261)
  AUTHORS   Gonzalez-Begne,M., Lu,B., Han,X., Hagen,F.K., Hand,A.R.,
            Melvin,J.E. and Yates,J.R.
  TITLE     Proteomic analysis of human parotid gland exosomes by
            multidimensional protein identification technology (MudPIT)
  JOURNAL   J Proteome Res 8 (3), 1304-1314 (2009)
   PUBMED   19199708
REFERENCE   7  (bases 1 to 2261)
  AUTHORS   Agromayor,M., Carlton,J.G., Phelan,J.P., Matthews,D.R.,
            Carlin,L.M., Ameer-Beg,S., Bowers,K. and Martin-Serrano,J.
  TITLE     Essential role of hIST1 in cytokinesis
  JOURNAL   Mol Biol Cell 20 (5), 1374-1387 (2009)
   PUBMED   19129480
REFERENCE   8  (bases 1 to 2261)
  AUTHORS   Bajorek,M., Morita,E., Skalicky,J.J., Morham,S.G., Babst,M. and
            Sundquist,W.I.
  TITLE     Biochemical analyses of human IST1 and its function in cytokinesis
  JOURNAL   Mol Biol Cell 20 (5), 1360-1373 (2009)
   PUBMED   19129479
REFERENCE   9  (bases 1 to 2261)
  AUTHORS   Gonzales,P.A., Pisitkun,T., Hoffert,J.D., Tchapyjnikov,D.,
            Star,R.A., Kleta,R., Wang,N.S. and Knepper,M.A.
  TITLE     Large-scale proteomics and phosphoproteomics of urinary exosomes
  JOURNAL   J Am Soc Nephrol 20 (2), 363-379 (2009)
   PUBMED   19056867
REFERENCE   10 (bases 1 to 2261)
  AUTHORS   Hoja,M.R., Wahlestedt,C. and Hoog,C.
  TITLE     A visual intracellular classification strategy for uncharacterized
            human proteins
  JOURNAL   Exp Cell Res 259 (1), 239-246 (2000)
   PUBMED   10942595
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000019.1 and BC092631.1.
            
            On Jul 21, 2009 this sequence version replaced NM_001017454.1.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC092631.1, SRR26643292.61473.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA5760383,
                                           SAMEA5760389 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-43                JAXUCZ010000019.1  54580449-54580491   c
            44-2253             BC092631.1         1-2210
            2254-2261           JAXUCZ010000019.1  54558353-54558360   c
FEATURES             Location/Qualifiers
     source          1..2261
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="19"
                     /map="19q12"
     gene            1..2261
                     /gene="Ist1"
                     /gene_synonym="CHMP8; RGD1307799"
                     /note="IST1 factor associated with ESCRT-III"
                     /db_xref="GeneID:307833"
                     /db_xref="RGD:1307799"
     exon            1..96
                     /gene="Ist1"
                     /gene_synonym="CHMP8; RGD1307799"
                     /inference="alignment:Splign:2.1.0"
     exon            97..199
                     /gene="Ist1"
                     /gene_synonym="CHMP8; RGD1307799"
                     /inference="alignment:Splign:2.1.0"
     CDS             112..1212
                     /gene="Ist1"
                     /gene_synonym="CHMP8; RGD1307799"
                     /note="MAPK activating protein PM28; IST1, ESCRT-III
                     associated factor; increased sodium tolerance 1 homolog;
                     charged multivesicular body protein 8"
                     /codon_start=1
                     /product="IST1 homolog"
                     /protein_id="NP_001017454.1"
                     /db_xref="GeneID:307833"
                     /db_xref="RGD:1307799"
                     /translation="MLGSGFKAERLRVNLRLVINRLKLLEKKKTELAQKARKEIADYL
                     AAGKDERARIRVEHIIREDYLVEAMEILELYCDLLLARFGLIQSMKELDSGLAESVST
                     LIWAAPRLQSEVAELKIVADQLCAKYSKEYGKLCRTNQIGTVNDRLMHKLSVEAPPKI
                     LVERYLIEIAKNYNVPYEPDSVVMAEAPVGVETDLIDVGFTDDVKKGGPGRGGGGGFT
                     APVGAPDGTMPMPMPMPMPMPSPSPNAPFAYPLPKGPSDFSGLPVGTYQAFPNIHPPQ
                     IPATPPSYESVDDINADKNVSSAQIVGPKPEAPAKPPSRPVDNYNTFVLPELPSVPDT
                     LPTASAGASTSASEDIDFDDLSRRFEELKKKT"
     exon            200..380
                     /gene="Ist1"
                     /gene_synonym="CHMP8; RGD1307799"
                     /inference="alignment:Splign:2.1.0"
     exon            381..468
                     /gene="Ist1"
                     /gene_synonym="CHMP8; RGD1307799"
                     /inference="alignment:Splign:2.1.0"
     exon            469..552
                     /gene="Ist1"
                     /gene_synonym="CHMP8; RGD1307799"
                     /inference="alignment:Splign:2.1.0"
     exon            553..663
                     /gene="Ist1"
                     /gene_synonym="CHMP8; RGD1307799"
                     /inference="alignment:Splign:2.1.0"
     exon            664..876
                     /gene="Ist1"
                     /gene_synonym="CHMP8; RGD1307799"
                     /inference="alignment:Splign:2.1.0"
     exon            877..969
                     /gene="Ist1"
                     /gene_synonym="CHMP8; RGD1307799"
                     /inference="alignment:Splign:2.1.0"
     exon            970..1018
                     /gene="Ist1"
                     /gene_synonym="CHMP8; RGD1307799"
                     /inference="alignment:Splign:2.1.0"
     exon            1019..2261
                     /gene="Ist1"
                     /gene_synonym="CHMP8; RGD1307799"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 agtcctctgt ggtgaggtcg agggaatcgc cattttggat gcagaacggc taatcggaaa
       61 tctgcttctt ccgttttcgc gtctggatcc ctccaggagg agcaggacaa catgctgggc
      121 tctggtttca aagctgagcg cttacgagtc aatttgagat tagttataaa tcgtcttaaa
      181 ctgttggaga aaaaaaaaac ggaactggct caaaaagcga ggaaggagat tgctgactat
      241 ctggccgctg ggaaggacga gagagcacgt atccgtgtgg agcacataat ccgggaagac
      301 taccttgtgg aggccatgga gatcctggag ctgtactgtg acctgctgct ggctcggttt
      361 ggccttatcc agtctatgaa ggagctagat tctggtctgg ctgaatctgt gtctacactg
      421 atctgggccg ctcctcggtt gcagtcagaa gtggctgagt tgaaaatagt tgctgatcag
      481 ctctgtgcca aatacagcaa ggaatatggc aagttgtgca ggaccaacca gatcgggact
      541 gtgaatgata ggctaatgca taaactgagt gtagaagccc cacccaagat cctagtggag
      601 agatacctga ttgaaattgc caagaattac aatgtgccct atgaacctga ctctgtggtc
      661 atggcagaag ctcccgttgg agtggagaca gatcttattg atgttggatt tacagatgat
      721 gtgaagaaag gtggtcctgg aagaggagga gggggcgggt tcacagcccc agttggtgca
      781 cctgatggga ccatgcccat gcccatgccc atgcccatgc ccatgccatc gccatctcca
      841 aatgctccct tcgcataccc actgccaaag ggaccatcgg atttcagtgg attgccagtg
      901 gggacttacc aggcctttcc taacattcac ccaccccaga taccagcaac tcccccatct
      961 tacgaatctg ttgatgacat taatgctgat aaaaatgttt cttcggcaca gattgttgga
     1021 cccaagccag aagctcctgc aaagccccct tccagacctg tggataacta caacaccttt
     1081 gtactgccag agttgccatc ggtgccagac acactcccga ccgcatctgc cggagccagt
     1141 acctcggcgt cagaggacat agactttgat gatctttccc ggagatttga ggagctgaaa
     1201 aagaaaacat aggtctctta aaccaggcaa catccacgat ctggaagttg atatggaaca
     1261 gttctccttg taacaatgaa tctccatgaa ttccgtttcc tctgttaacc gtcactgagc
     1321 actcctcctc ggggctcctg ctgctctacc aactgtgttg ctttccagtt ctccagagat
     1381 cctgagagac taacagctag aggctctggc tgtgtttgcc tgtctgtctt cctctcacat
     1441 ggatcccttg gttctttcct ggccatttca ttcacaggga gtgaggagta ttgcgggtag
     1501 aggtgccaaa gatggctgga ccatgaagga gaggacccta tgatgagttt caaccttgac
     1561 ttgtaaatac tttcctctgc atgggggcga ttgtagaaag aatcaaggct accatgtgtg
     1621 ttctcaatga ctcagaaagc cacggtcacg gttcctggag gctgggccct cactgacatg
     1681 tacactcctc cttgctgcag ggcactgctc cacctggatc cagttgcaaa gtgtgtttgg
     1741 aaaagttctt ggtcttactg gattctcagg gagccctctg tggccttttc ctttattttg
     1801 agtgctgttt cctttctagg aaagagcagc actttggaca tttgtttttt ccccatagca
     1861 tgttaggaag gacgtgctgg gcttccagtt gccttaatag aagtactcaa gtctcttggg
     1921 tagagatctg gaagcccccg ggacaggagg ggcacgtgtt ttcattcgaa aacttttatg
     1981 attaagggac tctttacaaa ctgctatctt gagttttagt gccatgtagc tgtagttcac
     2041 tgctttcctg gtggatgaga ctttcttagg ataacttctg tgctgctttg ccctggccaa
     2101 ggctgccctt ccccaagagg ttagctgtgg gattggaaga tgagattttt gtaatatttc
     2161 tgtacagacc tgtttccagc aacttcagtg tcaatgtttt tgatttattt gtagctcata
     2221 gctctagctg ctaataaaga tcccatctcc tgctttctct a
//
