LOCUS       NM_001105936             924 bp    mRNA    linear   ROD 12-JUN-2023
DEFINITION  Rattus norvegicus iron-sulfur cluster assembly enzyme (Iscu), mRNA.
ACCESSION   NM_001105936 XM_001078081 XM_213811
VERSION     NM_001105936.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 924)
  AUTHORS   He H, Liu J, Li W, Yao X, Ren Q, Shen B, Xue C, Zou L, Zhao H, Qin
            J and Jin G.
  TITLE     MiR-210-3p Inhibits Proliferation and Migration of C6 Cells by
            Targeting Iscu
  JOURNAL   Neurochem Res 45 (8), 1813-1824 (2020)
   PUBMED   32388695
  REMARK    GeneRIF: MiR-210-3p Inhibits Proliferation and Migration of C6
            Cells by Targeting Iscu.
REFERENCE   2  (bases 1 to 924)
  AUTHORS   Ma Q, Dasgupta C, Li Y, Huang L and Zhang L.
  TITLE     MicroRNA-210 Downregulates ISCU and Induces Mitochondrial
            Dysfunction and Neuronal Death in Neonatal Hypoxic-Ischemic Brain
            Injury
  JOURNAL   Mol Neurobiol 56 (8), 5608-5625 (2019)
   PUBMED   30656514
  REMARK    GeneRIF: miR-210 by downregulating ISCU and inducing mitochondrial
            dysfunction in neurons is a potent contributor of oxidative brain
            injury after neonatal Hypoxic-Ischemia.
REFERENCE   3  (bases 1 to 924)
  AUTHORS   Shan Y and Cortopassi G.
  TITLE     Mitochondrial Hspa9/Mortalin regulates erythroid differentiation
            via iron-sulfur cluster assembly
  JOURNAL   Mitochondrion 26, 94-103 (2016)
   PUBMED   26702583
REFERENCE   4  (bases 1 to 924)
  AUTHORS   Maio N and Rouault TA.
  TITLE     Iron-sulfur cluster biogenesis in mammalian cells: New insights
            into the molecular mechanisms of cluster delivery
  JOURNAL   Biochim Biophys Acta 1853 (6), 1493-1512 (2015)
   PUBMED   25245479
  REMARK    Review article
REFERENCE   5  (bases 1 to 924)
  AUTHORS   McCormick RI, Blick C, Ragoussis J, Schoedel J, Mole DR, Young AC,
            Selby PJ, Banks RE and Harris AL.
  TITLE     miR-210 is a target of hypoxia-inducible factors 1 and 2 in renal
            cancer, regulates ISCU and correlates with good prognosis
  JOURNAL   Br J Cancer 108 (5), 1133-1142 (2013)
   PUBMED   23449350
REFERENCE   6  (bases 1 to 924)
  AUTHORS   Favaro E, Ramachandran A, McCormick R, Gee H, Blancher C, Crosby M,
            Devlin C, Blick C, Buffa F, Li JL, Vojnovic B, Pires das Neves R,
            Glazer P, Iborra F, Ivan M, Ragoussis J and Harris AL.
  TITLE     MicroRNA-210 regulates mitochondrial free radical response to
            hypoxia and krebs cycle in cancer cells by targeting iron sulfur
            cluster protein ISCU
  JOURNAL   PLoS One 5 (4), e10345 (2010)
   PUBMED   20436681
  REMARK    Publication Status: Online-Only
REFERENCE   7  (bases 1 to 924)
  AUTHORS   Martelli A, Wattenhofer-Donze M, Schmucker S, Bouvet S, Reutenauer
            L and Puccio H.
  TITLE     Frataxin is essential for extramitochondrial Fe-S cluster proteins
            in mammalian tissues
  JOURNAL   Hum Mol Genet 16 (22), 2651-2658 (2007)
   PUBMED   17597094
REFERENCE   8  (bases 1 to 924)
  AUTHORS   Li K, Tong WH, Hughes RM and Rouault TA.
  TITLE     Roles of the mammalian cytosolic cysteine desulfurase, ISCS, and
            scaffold protein, ISCU, in iron-sulfur cluster assembly
  JOURNAL   J Biol Chem 281 (18), 12344-12351 (2006)
   PUBMED   16527810
REFERENCE   9  (bases 1 to 924)
  AUTHORS   Mootha VK, Bunkenborg J, Olsen JV, Hjerrild M, Wisniewski JR, Stahl
            E, Bolouri MS, Ray HN, Sihag S, Kamal M, Patterson N, Lander ES and
            Mann M.
  TITLE     Integrated analysis of protein composition, tissue diversity, and
            gene regulation in mouse mitochondria
  JOURNAL   Cell 115 (5), 629-640 (2003)
   PUBMED   14651853
REFERENCE   10 (bases 1 to 924)
  AUTHORS   Tong WH and Rouault T.
  TITLE     Distinct iron-sulfur cluster assembly complexes exist in the
            cytosol and mitochondria of human cells
  JOURNAL   EMBO J 19 (21), 5692-5700 (2000)
   PUBMED   11060020
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from CH473973.1.
            
            On or before Oct 3, 2007 this sequence version replaced
            XM_001078081.1, XM_213811.4.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC167056.1, CF108498.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5756307, SAMEA5760383
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: inferred from homology
            RefSeq Select criteria             :: based on single
                                                  protein-coding transcript
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..924
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10116"
                     /chromosome="12"
                     /map="12q16"
     gene            1..924
                     /gene="Iscu"
                     /gene_synonym="RGD1309562"
                     /note="iron-sulfur cluster assembly enzyme"
                     /db_xref="GeneID:288740"
                     /db_xref="RGD:1309562"
     exon            1..155
                     /gene="Iscu"
                     /gene_synonym="RGD1309562"
                     /inference="alignment:Splign:2.1.0"
     CDS             42..545
                     /gene="Iscu"
                     /gene_synonym="RGD1309562"
                     /note="IscU iron-sulfur cluster scaffold homolog;
                     iron-sulfur cluster assembly enzyme ISCU, mitochondrial"
                     /codon_start=1
                     /product="iron-sulfur cluster assembly enzyme ISCU"
                     /protein_id="NP_001099406.1"
                     /db_xref="GeneID:288740"
                     /db_xref="RGD:1309562"
                     /translation="MAAAGAGRLRRAASALLLRSPRLPARELSAPARLYHKKVVDHYE
                     NPRNVGSLDKTSKNVGTGLVGAPACGDVMKLQIQVDEKGKIVDARFKTFGCGSAIASS
                     SLATEWVKGKTVEEALTIKNTDIAKELCLPPVKLHCSMLAEDAIKAALADYKLKQEAK
                     KDEPEKQ"
     exon            156..269
                     /gene="Iscu"
                     /gene_synonym="RGD1309562"
                     /inference="alignment:Splign:2.1.0"
     exon            270..380
                     /gene="Iscu"
                     /gene_synonym="RGD1309562"
                     /inference="alignment:Splign:2.1.0"
     exon            381..459
                     /gene="Iscu"
                     /gene_synonym="RGD1309562"
                     /inference="alignment:Splign:2.1.0"
     exon            460..924
                     /gene="Iscu"
                     /gene_synonym="RGD1309562"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 cttcggcgtc gcagaggtcg acgcaggtgc aagccggcaa gatggcggcg gctggagcgg
       61 gccgtctgag gcgggcggcg tccgcgctgc tgctccggag cccgcgcctg cccgcccggg
      121 agttgtcggc tcctgccagg ctctaccaca agaaggttgt ggatcattat gaaaacccta
      181 ggaacgtggg gtcccttgac aagacatcta aaaatgttgg aaccggactg gtgggggctc
      241 cggcatgtgg tgacgtcatg aaactgcaga tccaggtgga tgaaaagggg aagattgtgg
      301 atgccagatt taaaacattt ggctgcggct ccgccattgc ctccagctcc ttagccacgg
      361 agtgggtaaa ggggaaaacg gtggaggaag ccctgaccat caaaaacaca gacatcgcca
      421 aggaactctg cctcccgcct gtgaaactgc attgctccat gctggcagaa gatgccatca
      481 aggctgccct ggctgattac aaactgaaac aagaggccaa gaaggacgag ccggagaagc
      541 agtgagccct gaggacgctc cagccgctca cagcaggcct cctgccaccc tgcacatcag
      601 agaagctacg caaaacgcac actactcact gtttgcacta tggctctgat acaagcaaaa
      661 tgtgccgttg attacgccta atcctgtgtt cctttcagct cacgtgtgtc cttagtgcag
      721 ttgatgtctt gattgagttt gtgacctggg actgaaatca gcaccaaagt cttaagctct
      781 ggagagtgac agggagttgt acccaagctg cggggagacg gccagcagag caccttgtgc
      841 tggctctgat aggatctatc actgtgtgtt tagcagatct gcgtgtatat tataataaaa
      901 aatggaagac gatggttttc tcta
//
