LOCUS       NM_001109278             801 bp    mRNA    linear   ROD 19-SEP-2024
DEFINITION  Rattus norvegicus iron-sulfur cluster assembly 2 (Isca2), mRNA;
            nuclear gene for mitochondrial product.
ACCESSION   NM_001109278 XM_001059413 XM_576075
VERSION     NM_001109278.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 801)
  AUTHORS   Morgenstern,M., Peikert,C.D., Lubbert,P., Suppanz,I., Klemm,C.,
            Alka,O., Steiert,C., Naumenko,N., Schendzielorz,A., Melchionda,L.,
            Muhlhauser,W.W.D., Knapp,B., Busch,J.D., Stiller,S.B.,
            Dannenmaier,S., Lindau,C., Licheva,M., Eickhorst,C., Galbusera,R.,
            Zerbes,R.M., Ryan,M.T., Kraft,C., Kozjak-Pavlovic,V., Drepper,F.,
            Dennerlein,S., Oeljeklaus,S., Pfanner,N., Wiedemann,N. and
            Warscheid,B.
  TITLE     Quantitative high-confidence human mitochondrial proteome and its
            dynamics in cellular context
  JOURNAL   Cell Metab 33 (12), 2464-2483 (2021)
   PUBMED   34800366
REFERENCE   2  (bases 1 to 801)
  AUTHORS   Maio,N. and Rouault,T.A.
  TITLE     Iron-sulfur cluster biogenesis in mammalian cells: New insights
            into the molecular mechanisms of cluster delivery
  JOURNAL   Biochim Biophys Acta 1853 (6), 1493-1512 (2015)
   PUBMED   25245479
  REMARK    Review article
REFERENCE   3  (bases 1 to 801)
  AUTHORS   Sheftel,A.D., Wilbrecht,C., Stehling,O., Niggemeyer,B.,
            Elsasser,H.P., Muhlenhoff,U. and Lill,R.
  TITLE     The human mitochondrial ISCA1, ISCA2, and IBA57 proteins are
            required for [4Fe-4S] protein maturation
  JOURNAL   Mol Biol Cell 23 (7), 1157-1166 (2012)
   PUBMED   22323289
  REMARK    GeneRIF: Eukaryotic iron-sulfer cluster proteins are found in
            mitochondria.
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000006.1, FN806632.1 and FN806631.1.
            
            On Apr 12, 2013 this sequence version replaced NM_001109278.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AI059493.1, AW522032.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760383, SAMEA5760386
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: PMID: 22323289
            RefSeq Select criteria             :: based on single
                                                  protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-3                 JAXUCZ010000006.1  110149593-110149595
            4-406               FN806632.1         1-403
            407-801             FN806631.1         176-570
FEATURES             Location/Qualifiers
     source          1..801
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="6"
                     /map="6q31"
     gene            1..801
                     /gene="Isca2"
                     /gene_synonym="Hbld1; RGD1563216"
                     /note="iron-sulfur cluster assembly 2"
                     /db_xref="GeneID:500694"
                     /db_xref="RGD:1563216"
     CDS             1..465
                     /gene="Isca2"
                     /gene_synonym="Hbld1; RGD1563216"
                     /note="HESB like domain containing 1; iron-sulfur cluster
                     assembly 2 homolog, mitochondrial"
                     /codon_start=1
                     /product="iron-sulfur cluster assembly 2 homolog,
                     mitochondrial precursor"
                     /protein_id="NP_001102748.1"
                     /db_xref="GeneID:500694"
                     /db_xref="RGD:1563216"
                     /translation="MAAARALSLTAEAFGAAIPRRQGRLLAAFPRLLTRWETTSSIPE
                     AGEGQIRLTDSCVQRLLEITEGSEFLRLQVEGGGCSGFQYKFSLDTVINPDDRVFEQG
                     GARVVVDSDSLAYVKGAQVDFSQELIRSSFQVLNNPQAQQGCSCGSSFSIKI"
     exon            1..71
                     /gene="Isca2"
                     /gene_synonym="Hbld1; RGD1563216"
                     /inference="alignment:Splign:2.1.0"
     exon            72..174
                     /gene="Isca2"
                     /gene_synonym="Hbld1; RGD1563216"
                     /inference="alignment:Splign:2.1.0"
     exon            175..290
                     /gene="Isca2"
                     /gene_synonym="Hbld1; RGD1563216"
                     /inference="alignment:Splign:2.1.0"
     exon            291..801
                     /gene="Isca2"
                     /gene_synonym="Hbld1; RGD1563216"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 atggcggccg ccagggcctt gtccctaact gctgaggcgt tcggggccgc cattcctagg
       61 cgccagggaa ggctcctcgc cgcctttccc aggcttttga cccgctggga aacaacgtcc
      121 tccattccag aggctggcga gggacagatc cgcctcacag acagctgcgt ccagaggctt
      181 ctggaaatca ccgaagggtc agaattcctc aggctgcaag tagagggagg tggatgctcc
      241 ggattccaat acaaattttc actggacaca gttattaacc ccgacgacag ggtatttgaa
      301 cagggtgggg caagagtggt ggttgactct gatagcttgg cctacgttaa gggggcccag
      361 gtggacttca gccaagaact aatccgaagc tcattccagg tgttgaataa tcctcaagcc
      421 cagcaaggct gctcctgtgg gtcatccttc tctatcaaaa tctgacacaa tggcagatga
      481 ctctgggatt gccaccattg tgccagtgtg aaaaacccgg gaatagtaga attctagaga
      541 tcgatttctc atcatgtccc cctctcattt aatttagtcc agcggtgtct tattttacca
      601 ctattaatta tggaatgtga agcttttact cttgacagaa atactctccc ctcccccagc
      661 acaatgttaa ggccaaatcc aaatgccgtt acctcaacct taatcactgg ttggaaccct
      721 agcacttagt acctctagga ctccaaaatt tggagttaac ttctctagcc ttcaaaattg
      781 tttatatgtg tgtgtatagc t
//
