LOCUS       NM_001105210            6182 bp    mRNA    linear   ROD 26-FEB-2024
DEFINITION  Rattus norvegicus inositol 1,4,5-triphosphate receptor associated 1
            (Irag1), transcript variant 2, mRNA.
ACCESSION   NM_001105210 XM_001074012 XM_218999
VERSION     NM_001105210.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 6182)
  AUTHORS   Geiselhoringer A, Werner M, Sigl K, Smital P, Worner R, Acheo L,
            Stieber J, Weinmeister P, Feil R, Feil S, Wegener J, Hofmann F and
            Schlossmann J.
  TITLE     IRAG is essential for relaxation of receptor-triggered smooth
            muscle contraction by cGMP kinase
  JOURNAL   EMBO J 23 (21), 4222-4231 (2004)
   PUBMED   15483626
  REMARK    GeneRIF: Functional analysis of a similar gene in mouse.
REFERENCE   2  (bases 1 to 6182)
  AUTHORS   Shaughnessy JD Jr, Largaespada DA, Tian E, Fletcher CF, Cho BC,
            Vyas P, Jenkins NA and Copeland NG.
  TITLE     Mrvi1, a common MRV integration site in BXH2 myeloid leukemias,
            encodes a protein with homology to a lymphoid-restricted membrane
            protein Jaw1
  JOURNAL   Oncogene 18 (12), 2069-2084 (1999)
   PUBMED   10321731
  REMARK    GeneRIF: Demonstrates alternative splicing in the similar mouse and
            human genes, and the use of a non-AUG (CUG) translation initiation
            codon in one of the transcript variants.
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from CH473956.2.
            
            On or before Sep 22, 2007 this sequence version replaced
            XM_218999.4, XM_001074012.1.
            
            Summary: This gene is similar to a putative mouse tumor suppressor
            gene that is frequently disrupted by mouse AIDS-related virus
            (MRV). The encoded protein, which is found in the membrane of the
            endoplasmic reticulum, is similar to Jaw1, a lymphoid-restricted
            protein whose expression is down-regulated during lymphoid
            differentiation. Studies in mouse suggest that MRV integration at
            the homologous mouse gene induces myeloid leukemia by altering the
            expression of a gene important for myeloid cell growth and/or
            differentiation, and thus this gene may function as a myeloid
            leukemia tumor suppressor gene. Alternatively spliced transcript
            variants have been found for this gene. [provided by RefSeq, Nov
            2013].
            
            Transcript Variant: This variant (2) contains an additional 5'
            exon, is differently spliced at the initial coding exon, and
            initiates translation from an in-frame, downstream AUG compared to
            variant 1. The resulting isoform (b) has a shorter N-terminus
            compared to isoform a.
            
            Sequence Note: This RefSeq transcript and protein were derived from
            genomic sequence because no rat transcript was available. The
            extent of this transcript and the encoded protein are supported by
            orthologous mouse data.
            
            ##Evidence-Data-START##
            RNAseq introns :: mixed sample support SAMD00132273, SAMD00132292
                              [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            inferred exon combination :: based on alignments, homology
            RefSeq Select criteria    :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
FEATURES             Location/Qualifiers
     source          1..6182
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10116"
                     /chromosome="1"
                     /map="1q33"
     gene            1..6182
                     /gene="Irag1"
                     /gene_synonym="Mrvi1"
                     /note="inositol 1,4,5-triphosphate receptor associated 1"
                     /db_xref="GeneID:308899"
                     /db_xref="RGD:1309828"
     exon            1..443
                     /gene="Irag1"
                     /gene_synonym="Mrvi1"
                     /inference="alignment:Splign:2.1.0"
     exon            444..592
                     /gene="Irag1"
                     /gene_synonym="Mrvi1"
                     /inference="alignment:Splign:2.1.0"
     CDS             533..3100
                     /gene="Irag1"
                     /gene_synonym="Mrvi1"
                     /note="isoform b is encoded by transcript variant 2; MRV
                     integration site 1 homolog; protein MRVI1; murine
                     retrovirus integration site 1 homolog"
                     /codon_start=1
                     /product="inositol 1,4,5-triphosphate receptor associated
                     1 isoform b"
                     /protein_id="NP_001098680.1"
                     /db_xref="GeneID:308899"
                     /db_xref="RGD:1309828"
                     /translation="MPHIPEDEEPPGEPQAAQTQDSPSAGPFCSPPTIILTGDASSPG
                     GETDTNLVKRAPSPHRRLSHRHLKVSTASLTSVDPLGHVIDLVNDQLPDISISEEDKK
                     KNLALLEEAKLVSERFLTRRGRRSRSSLGDAPSAVSPNLSSGASPASSRSCSFTISTP
                     PGFDTCSGPQSPLPGSPAQQKGHEDDVFSSHLGEPNASKGLADRKQNDQRKVSQGRLA
                     PRSPTVEKSKELSVEQKENFDPLQHLEATPMAQTSGASVSGKMALNSPQPGPAEMELG
                     RQLLKTAREDNPLPRTTVQGSGGTASPHSQGQGSAGELMGPKAGSKAEPRPPVSRPPL
                     IRGVSWDSSPEEPGPLLQKVLAKLPLAEEEKRFPGKAKPAKPPGLKDFQIQVQPVRMQ
                     KLTKLREEHILMRNQNLVGFKLPELSEAAEQDKGVSSELAPAAEEEESKSGLDVMPNI
                     SDILLRKLRVHKSLTGSAPPLTEKEVENVFVQLSLAFRNDSYTLESRINQAERERNLT
                     EENTEKELENFKASITSSASIWYHCEHRETYQKLLEDIAVLHRLAARLSSRAEVVGAV
                     RQEKRMSKATEVMMQYVENLKRTYEKDHAELMEFKKLANQNSSRSCGPSEDGVPRTAR
                     SMSLTMGKNMPRRRVSVAVVPKFNALNLPGQAPSSSPMPSLPALSESTNGRSSISTSP
                     VLPALLENGKTNAEANCEVCAPVPLPSYPEETSQEAKARAEEEAYNKGYQEGVKKTEE
                     LQDLKEEEEEQKAESPQEPEEVEETQEDEKDQRSSKLEELVHFLQVMYPKLCQHWQVI
                     WMMAAVMLVLSVVLGLYSSYNSCTEQADGPPGRSTCSAAQRDSWWSSGLQQELPAEQ"
     exon            593..693
                     /gene="Irag1"
                     /gene_synonym="Mrvi1"
                     /inference="alignment:Splign:2.1.0"
     exon            694..764
                     /gene="Irag1"
                     /gene_synonym="Mrvi1"
                     /inference="alignment:Splign:2.1.0"
     exon            765..938
                     /gene="Irag1"
                     /gene_synonym="Mrvi1"
                     /inference="alignment:Splign:2.1.0"
     exon            939..1016
                     /gene="Irag1"
                     /gene_synonym="Mrvi1"
                     /inference="alignment:Splign:2.1.0"
     exon            1017..1069
                     /gene="Irag1"
                     /gene_synonym="Mrvi1"
                     /inference="alignment:Splign:2.1.0"
     exon            1070..1117
                     /gene="Irag1"
                     /gene_synonym="Mrvi1"
                     /inference="alignment:Splign:2.1.0"
     exon            1118..1729
                     /gene="Irag1"
                     /gene_synonym="Mrvi1"
                     /inference="alignment:Splign:2.1.0"
     exon            1730..1808
                     /gene="Irag1"
                     /gene_synonym="Mrvi1"
                     /inference="alignment:Splign:2.1.0"
     exon            1809..1932
                     /gene="Irag1"
                     /gene_synonym="Mrvi1"
                     /inference="alignment:Splign:2.1.0"
     exon            1933..1963
                     /gene="Irag1"
                     /gene_synonym="Mrvi1"
                     /inference="alignment:Splign:2.1.0"
     exon            1964..2104
                     /gene="Irag1"
                     /gene_synonym="Mrvi1"
                     /inference="alignment:Splign:2.1.0"
     exon            2105..2236
                     /gene="Irag1"
                     /gene_synonym="Mrvi1"
                     /inference="alignment:Splign:2.1.0"
     exon            2237..2378
                     /gene="Irag1"
                     /gene_synonym="Mrvi1"
                     /inference="alignment:Splign:2.1.0"
     exon            2379..2428
                     /gene="Irag1"
                     /gene_synonym="Mrvi1"
                     /inference="alignment:Splign:2.1.0"
     exon            2429..2536
                     /gene="Irag1"
                     /gene_synonym="Mrvi1"
                     /inference="alignment:Splign:2.1.0"
     exon            2537..2601
                     /gene="Irag1"
                     /gene_synonym="Mrvi1"
                     /inference="alignment:Splign:2.1.0"
     exon            2602..2721
                     /gene="Irag1"
                     /gene_synonym="Mrvi1"
                     /inference="alignment:Splign:2.1.0"
     exon            2722..2856
                     /gene="Irag1"
                     /gene_synonym="Mrvi1"
                     /inference="alignment:Splign:2.1.0"
     exon            2857..6182
                     /gene="Irag1"
                     /gene_synonym="Mrvi1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      6160..6165
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Irag1"
                     /gene_synonym="Mrvi1"
     polyA_site      6182
                     /gene="Irag1"
                     /gene_synonym="Mrvi1"
ORIGIN      
        1 agacagagtc cagcatcttc aactgccccc caaggcactc atcagctatg ggggtgcgcc
       61 tgcaaaagcc cagggttagg ggctactatt caggcccaac tttccttgaa ccctgaggct
      121 ggctggaagg catctcctgc ttttagccca gaggcaggct gcagtgggcc ctggtggaac
      181 actgtttccc agtcccagcc cctcatccag cccctctctg aggaggaggc aggcagcagg
      241 gctctgcagc cagagctttg cagcttggag ctctggagcc agatgtaaca ttgaccttaa
      301 atggtaaaag ctccccagag tgaagagagg ctggccgggg aggaaagagg aataactcag
      361 ttttaggtaa gtcatcgcta ttgcctagaa taacctgcct cttcagcccg aggaaagcag
      421 agaggcgcct ctcctcctta aagcctgcgg agcccaagcc tcttggagca tctttggggt
      481 tgtcacagca gaggttccag gcacgcacag ccactccaac caggcggctg ccatgcccca
      541 cattcctgag gatgaggagc cccccggaga gccacaggca gcccagaccc aagactctcc
      601 ctctgcagga ccgttttgca gccctcccac aatcatcctg acgggggatg ccagttcacc
      661 tggaggagag actgacacaa atctggtcaa gagggctccc agcccccacc gcaggctttc
      721 ccaccgacac ctgaaggtct ccactgcctc gctgacttct gtggacccct tggggcacgt
      781 cattgacctg gtaaatgacc agctgccgga catcagcatc tcagaagagg acaagaagaa
      841 aaacctggca cttctagaag aagccaagtt ggttagcgag cggttcctga cccgccgcgg
      901 gaggagatca aggagcagcc tcggagacgc cccatcagct gtttctccaa acctcagctc
      961 tggagcttct cctgcatcct ctaggagttg ctcgttcacc atctccacac caccaggttt
     1021 tgacacatgc agtggcccac agtcccctct gccgggatca ccagcacagc aaaagggaca
     1081 tgaggatgat gtcttctcgt ctcacctcgg agagcctaat gcctccaaag ggctagctga
     1141 ccggaagcag aatgaccaga ggaaagtgtc tcaaggcaga ctggctcctc gctctcccac
     1201 agtggagaaa tccaaagaac tttcagtaga acaaaaggaa aacttcgatc cccttcagca
     1261 tctggaggcc acacccatgg ctcagacctc tggagcaagc gtcagtggga aaatggcgct
     1321 gaacagtccc cagcctggcc ctgcggagat ggagctgggg aggcagcttc tgaagacagc
     1381 cagggaagac aaccctctgc ccagaaccac agtccagggc tcaggaggaa cggcctcccc
     1441 acattctcag ggacagggct ctgctggaga gcttatgggg cccaaggctg gctccaaggc
     1501 tgagccgagg cctcctgtgt cccggccccc tctgattcgg ggggtctcct gggacagcag
     1561 ccctgaagaa cctggtcccc tgctgcaaaa ggtgctcgct aagctgcctc tggcagagga
     1621 agaaaagcgg tttccaggca aagccaagcc agccaagcca ccggggctca aagacttcca
     1681 gatacaagta cagccggtgc gcatgcagaa gctgaccaag cttcgggagg aacacatcct
     1741 gatgagaaac cagaacctgg tggggttcaa gctccctgaa ctgagtgaag ctgcagagca
     1801 agacaaagga gtctcatctg agcttgcccc agcagctgag gaagaagaat caaagagtgg
     1861 tctggatgtg atgcccaaca tttctgacat attgctacgt aaacttaggg ttcacaagtc
     1921 actcactgga agcgcccctc cgctcacaga aaaggaagtc gagaacgtgt ttgtacaact
     1981 gtccttggcc tttagaaatg acagctacac cttggagtct aggattaacc aggctgaaag
     2041 ggaacgcaac ttgacggaag agaacaccga gaaggaactg gaaaacttca aagcctctat
     2101 tacgtcctca gcatccatct ggtaccactg cgagcaccgg gagacctacc agaagcttct
     2161 ggaagacatc gcagtcttgc atcgtctggc tgcacgcctc tccagccggg ctgaagtggt
     2221 gggtgctgtg cgccaggaaa agcgcatgtc aaaggccacg gaagtgatga tgcagtatgt
     2281 ggagaatctg aagagaacgt acgagaagga ccatgctgag ctcatggaat ttaagaaact
     2341 cgcaaatcag aattctagtc gcagctgtgg gccctctgaa gacggggttc ctcgcacggc
     2401 gcgatctatg tccctcacga tgggaaagaa catgccccgt cggagggtca gcgttgctgt
     2461 ggttcccaag tttaatgccc tgaacctgcc tggccaagcc cccagctcat cacccatgcc
     2521 ctctctccca gctctgtctg aatcgaccaa cgggagaagc agcatctcca cctctccagt
     2581 gctgcctgca cttttggaaa atggaaagac aaatgcagag gctaactgtg aagtgtgcgc
     2641 ccctgtgcca ctcccaagct acccggagga gacgagccaa gaggccaagg ccagagcaga
     2701 ggaagaagcc tacaacaaag gataccagga aggggtaaag aagaccgaag aacttcaaga
     2761 cctgaaggag gaggaagagg aacagaaggc agagagtcct caggaaccag aagaggtcga
     2821 agaaacccag gaagacgaga aggaccagag aagcagcaaa cttgaagaac tggtccactt
     2881 cctacaagtc atgtatccca aattgtgtca acactggcaa gtgatctgga tgatggccgc
     2941 tgtgatgctg gtcttgagtg ttgtgcttgg gctctacagt tcctataact cctgcacgga
     3001 gcaggccgac gggccccctg ggagatccac ctgctctgca gcccagcggg actcctggtg
     3061 gagctcggga ctccagcaag agctgccagc agagcagtag gacatcggac tccctagcca
     3121 tgccctgctc tagtgcattt accccaacac cctgccccta ggtacagtgt gtcaggcccg
     3181 gtttgcacat gtggccaccc agcccattgg ggaacaagga ggctctctcc tgtcattcgt
     3241 ggaagcactc acagtaaccc gacgctttga agggatcaac aaaactgctc tgggaaaagt
     3301 gtctggttga caaagaagtg accttcgtta cggactctag cccaagggaa gagtcctgcc
     3361 cctggctcag atggattgag agaacgaaaa ccccttcact aggggaaaat gagaagctcg
     3421 ctttccccct tttcctccct gtgtttcgtg gtcagtaaga attgtactta gcggtcagta
     3481 ctgaccataa ggctcagact tggagtcaca acacagatat tgtcccactt cccacttcgt
     3541 gtatcacaac aatcctttca ccttctcttt ccctttcaga atcaccattt cttctcagag
     3601 agtgggaaag ctcgctcata aaaatacaca ggctaaaagt agccccatcc tagacaggag
     3661 acaggaagat gtggattaat atagaatgag tctgaataga tatggaccat acacacattt
     3721 ttgtgcacaa ggacacccac tctctgaaag tgtgctctgc tcagtggcac ccatgtcatg
     3781 ccaatcaatc ggttctcact aggtgtggag cagtagtcac caggcaccag cacagtgagc
     3841 cctccacaca ctccggagat gtttgaatcc cctgaggaat cgtgtcttca gatgccaggt
     3901 tggagctgct gtttggcaca gtctgttccc tggttgcttg cttcatttga aagtgatcct
     3961 acacagttaa cccccacctc ttgggtaaga aaaggtgtgc cctccacttc agactctcac
     4021 tctctgcctt gcacttcggg actacagttt tcatggtact tccaacccca atgtgtcctt
     4081 ccaaggaccc gttcagcgtc aaactgaagg cagtgtgtcc acctccagca tggtggctct
     4141 ccaccctgct cagtggttcc tttcattcag ctctcttcag ttggagatgg ggcttctctt
     4201 gtggttgctt ttctgtttca ttgtttttga tgctatgact catgtagaac gtattgtgag
     4261 ccaaaaacca tctgtgttat gtaggcagta tttccttagt cctcaagaaa aacaaaatct
     4321 gaaatggata tcactctctc catggtcaga taaggaaaca gaagcataaa tgatataaga
     4381 gtaaacacac acacacaaga gtgcatgcac atgcatatgc atatgcacac acatacaata
     4441 attcaactta agtcattcaa actacggtgg tctggctcca gggactgtac ttctgatcct
     4501 tcacctatgg gtgccttcgc cagctgggtg ccttccagtc tgatcctgtt ccccccagca
     4561 ggctcttcag ctgctccaat ccagctatgg ttcccaaatg acattgcccc tcctctcact
     4621 cggacccatc tttgctgctg tcgttcttgt ccttctcacc actcctgagg accaagtgta
     4681 agcactcccc tccatacagg cccctgtgac ctcttcccac actctcgaat gcccaacata
     4741 atgggcaaca ccctgttaga gcttctggta cttgttaaat tttaatcatc caaccatatt
     4801 tactgtctaa cttatttata catctgcctc cctggttgtt ttccctcaag aactatgctt
     4861 taccattcct tgtctacata cctagcatgg caaacccttg ggagagagtc atttaatgtt
     4921 ggttaaaaga acaagtgagc attcaaggta gagagaagcc tagaacacat gacgttttga
     4981 ggtggcccag tagtgtcacc ttcatgacga tttctattcc cgtgagaaag ctgtcataac
     5041 tggttcatgg tctcatctta tttttgtctt tcttggagag agcaagaatg aaatcacact
     5101 atagcgatac cattttcaag tccaagaacc tgattccatg cactcacccc cttcagagag
     5161 acacaccctt cccaccccca actaggcctc attttaaaat actgtttttg aagaattatc
     5221 tttacagtgg ctagaaaatg acagagagag agaagcccaa ggatgtgcca ttaaaggtaa
     5281 tgaggttcag agtgaggcag agggattttg caggtagggg tagggcttac ctggaggctt
     5341 ctgtgggatt agatacagtt gaaggagatt taactgcata agcacttctg agacaaagcc
     5401 agagtataca gggaatggag agagaacagc tgttgggcag gactctgtgt gtggtggggg
     5461 gagtgtctag tttcagtcta cccccacagc aaaagaagat ccagcctgag tgagcccatt
     5521 gtccacttgg ctgcaaatct cccacccctg ttctgggaga gacagagttg aggagaacat
     5581 gagtgaactg tttcagcgct gccactggcc tccgaagcca gcaggaatcc tcaggcacag
     5641 accagcaacc acgctgcttc agctgtaagg acagttaagg aaaaggacct ctccagagag
     5701 catagccttg tttggaatgg catggctgaa aacacacttg ttggtctgcc ctttcaggaa
     5761 agcgaggaac aacttagagc agccacagct gctgccacac atggccggtg acatggaagc
     5821 cagccttgtg tttccaggcg taactgtgaa atggaataaa agttcccact caagatgccc
     5881 ctgaaagctg acctttctgc ttaggcctga gagtctggtt gccagccttc tagtgccaag
     5941 gatgtgtttg catcatcact gacatcactt cctaagggga ccttcacgtt ctagccatga
     6001 agtcatatgg gctgtttcct ttgtgcagcc tagaagaggg tatggcaaag gagacactgg
     6061 acttacaggt catcggctgt ctgtccctaa tgctgttaaa cctcacacag gtgggtgtgg
     6121 ccgtgttgaa ctctagagtg tcacatagct gagtttgaaa ataaatgcac atttccaaac
     6181 tg
//
