LOCUS       NM_053680                595 bp    mRNA    linear   ROD 27-MAR-2024
DEFINITION  Rattus norvegicus insulin-like 3 (Insl3), mRNA.
ACCESSION   NM_053680
VERSION     NM_053680.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 595)
  AUTHORS   Xue,K., Kim,J.Y., Liu,J.Y. and Tsang,B.K.
  TITLE     Insulin-like 3-induced rat preantral follicular growth is mediated
            by growth differentiation factor 9
  JOURNAL   Endocrinology 155 (1), 156-167 (2014)
   PUBMED   24169563
  REMARK    GeneRIF: INSL3 is a key theca cell-derived growth factor for
            preantral follicle and its action is mediated by GDF9
REFERENCE   2  (bases 1 to 595)
  AUTHORS   Hampel,U., Klonisch,T., Sel,S., Schulze,U., Garreis,F.,
            Seitmann,H., Zouboulis,C.C. and Paulsen,F.P.
  TITLE     Insulin-like factor 3 promotes wound healing at the ocular surface
  JOURNAL   Endocrinology 154 (6), 2034-2045 (2013)
   PUBMED   23539510
  REMARK    Erratum:[Endocrinology. 2014 Jan;155(1):331. Garreis, Fabian
            [added]; Seitmann, Holger [added]; Zouboulis, Christos C [added]]
REFERENCE   3  (bases 1 to 595)
  AUTHORS   Hannas,B.R., Lambright,C.S., Furr,J., Howdeshell,K.L., Wilson,V.S.
            and Gray,L.E. Jr.
  TITLE     Dose-response assessment of fetal testosterone production and gene
            expression levels in rat testes following in utero exposure to
            diethylhexyl phthalate, diisobutyl phthalate, diisoheptyl
            phthalate, and diisononyl phthalate
  JOURNAL   Toxicol Sci 123 (1), 206-216 (2011)
   PUBMED   21633115
  REMARK    GeneRIF: The observed postnatal difference in gubernacular
            malformations between two rate strains is due to different levels
            of the insl3 receptor differ among strains.
REFERENCE   4  (bases 1 to 595)
  AUTHORS   Johnson,K.J., Robbins,A.K., Wang,Y., McCahan,S.M., Chacko,J.K. and
            Barthold,J.S.
  TITLE     Insulin-like 3 exposure of the fetal rat gubernaculum modulates
            expression of genes involved in neural pathways
  JOURNAL   Biol Reprod 83 (5), 774-782 (2010)
   PUBMED   20631401
  REMARK    GeneRIF: data suggest that INSL3 signaling in fetal gubernaculum
            induces morphogenetic programs in male genitalia; investigation of
            differences in gene expression regulation by INSL3 in gubernaculum
            of male and female genitalia
REFERENCE   5  (bases 1 to 595)
  AUTHORS   Zhang,W.J., Luo,X. and Guo,Z.Y.
  TITLE     In vitro degradation of insulin-like peptide 3 by insulin-degrading
            enzyme
  JOURNAL   Protein J 29 (2), 93-98 (2010)
   PUBMED   20082125
REFERENCE   6  (bases 1 to 595)
  AUTHORS   Kumagai,J., Hsu,S.Y., Matsumi,H., Roh,J.S., Fu,P., Wade,J.D.,
            Bathgate,R.A. and Hsueh,A.J.
  TITLE     INSL3/Leydig insulin-like peptide activates the LGR8 receptor
            important in testis descent
  JOURNAL   J Biol Chem 277 (35), 31283-31286 (2002)
   PUBMED   12114498
  REMARK    GeneRIF: Demonstration of INSL3 as the ligand for LGR8 facilitates
            understanding of the mechanism of testis descent and allows studies
            on the role of INSL3 in gonadal and other physiological processes
REFERENCE   7  (bases 1 to 595)
  AUTHORS   Boockfor,F.R., Fullbright,G., Bullesbach,E.E. and Schwabe,C.
  TITLE     Relaxin-like factor (RLF) serum concentrations and gubernaculum RLF
            receptor display in relation to pre- and neonatal development of
            rats
  JOURNAL   Reproduction 122 (6), 899-906 (2001)
   PUBMED   11732985
REFERENCE   8  (bases 1 to 595)
  AUTHORS   Smith,K.J., Wade,J.D., Claasz,A.A., Otvos,L. Jr., Temelcos,C.,
            Kubota,Y., Hutson,J.M., Tregear,G.W. and Bathgate,R.A.
  TITLE     Chemical synthesis and biological activity of rat INSL3
  JOURNAL   J Pept Sci 7 (9), 495-501 (2001)
   PUBMED   11587188
REFERENCE   9  (bases 1 to 595)
  AUTHORS   Spiess,A.N., Balvers,M., Tena-Sempere,M., Huhtaniemi,I., Parry,L.
            and Ivell,R.
  TITLE     Structure and expression of the rat relaxin-like factor (RLF) gene
  JOURNAL   Mol Reprod Dev 54 (4), 319-325 (1999)
   PUBMED   10542371
REFERENCE   10 (bases 1 to 595)
  AUTHORS   Zimmermann,S., Steding,G., Emmen,J.M., Brinkmann,A.O., Nayernia,K.,
            Holstein,A.F., Engel,W. and Adham,I.M.
  TITLE     Targeted disruption of the Insl3 gene causes bilateral
            cryptorchidism
  JOURNAL   Mol Endocrinol 13 (5), 681-691 (1999)
   PUBMED   10319319
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000016.1.
            
            On Jul 28, 2021 this sequence version replaced NM_053680.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF139918.2, SRR26643429.50245.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760383, SAMEA5760389
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-175               JAXUCZ010000016.1  18433189-18433363
            176-595             JAXUCZ010000016.1  18434114-18434533
FEATURES             Location/Qualifiers
     source          1..595
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="16"
                     /map="16p14"
     gene            1..595
                     /gene="Insl3"
                     /gene_synonym="Rlf"
                     /note="insulin-like 3"
                     /db_xref="GeneID:114215"
                     /db_xref="RGD:620117"
     CDS             1..387
                     /gene="Insl3"
                     /gene_synonym="Rlf"
                     /note="ley-I-L; relaxin-like factor; Leydig insulin-like
                     peptide relaxin-like factor"
                     /codon_start=1
                     /product="insulin-like 3 precursor"
                     /protein_id="NP_446132.1"
                     /db_xref="GeneID:114215"
                     /db_xref="RGD:620117"
                     /translation="MHALLLLLLLALGSALRSPQPPEARAKLCGHHLVRALVRVCGGP
                     RWSPEATQPVDTRDRELLQWLEQRHLLHALVADADPALDPDPALDPQLPHQASQRQRR
                     SVATNAVHRCCLTGCTQQDLLGLCPH"
     exon            1..175
                     /gene="Insl3"
                     /gene_synonym="Rlf"
                     /inference="alignment:Splign:2.1.0"
     exon            176..595
                     /gene="Insl3"
                     /gene_synonym="Rlf"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 atgcacgcac tgctgctact gctgctcctg gctctagggt ccgcgctgcg ctccccgcag
       61 cccccggagg cgcgcgccaa gctctgtggt caccacctgg tgcgtgcgct ggtgcgggtg
      121 tgcggtggcc cgcgttggtc acccgaggcc acgcagcctg tggacacccg cgaccgggag
      181 ctgctgcagt ggctggagca acgacatctc ctgcacgcgc tcgtggccga tgcggatccc
      241 gcgctagacc cggaccccgc gctggacccg cagcttcctc accaggcttc tcagcgccag
      301 cgccgcagtg tggccaccaa cgctgtgcac cgctgctgtc tcactggctg cacccagcaa
      361 gaccttttgg gtctgtgtcc ccactgaagg attgtagggc tcaggtggtg tgtgtcaatt
      421 attccccacc tcacagctgt gccaatgaaa cacccccgga ttgccttgga ggatccctag
      481 tttattcata gatcccagaa accaaggacc acaggtcctg tccccaggga gccccatcat
      541 gacctgtgcc cacctcctcc ctgtgggcca acagtctaaa taaaaaagca acttg
//
