LOCUS       NM_019130                464 bp    mRNA    linear   ROD 02-APR-2024
DEFINITION  Rattus norvegicus insulin 2 (Ins2), mRNA.
ACCESSION   NM_019130
VERSION     NM_019130.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 464)
  AUTHORS   Harvey,K.E., LaVigne,E.K., Dar,M.S., Salyer,A.E., Pratt,E.P.S.,
            Sample,P.A., Aryal,U.K., Gowher,H. and Hockerman,G.H.
  TITLE     RyR2/IRBIT regulates insulin gene transcript, insulin content, and
            secretion in the insulinoma cell line INS-1
  JOURNAL   Sci Rep 12 (1), 7713 (2022)
   PUBMED   35562179
  REMARK    GeneRIF: RyR2/IRBIT regulates insulin gene transcript, insulin
            content, and secretion in the insulinoma cell line INS-1.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 464)
  AUTHORS   Toyoshima,Y., Nakamura,K., Tokita,R., Teramoto,N., Sugihara,H.,
            Kato,H., Yamanouchi,K. and Minami,S.
  TITLE     Disruption of insulin receptor substrate-2 impairs growth but not
            insulin function in rats
  JOURNAL   J Biol Chem 295 (33), 11914-11927 (2020)
   PUBMED   32631952
  REMARK    GeneRIF: Disruption of insulin receptor substrate-2 impairs growth
            but not insulin function in rats.
REFERENCE   3  (bases 1 to 464)
  AUTHORS   Sekido,T., Nishio,S.I., Ohkubo,Y., Sekido,K., Kitahara,J.,
            Miyamoto,T. and Komatsu,M.
  TITLE     Repression of insulin gene transcription by indirect genomic
            signaling via the estrogen receptor in pancreatic beta cells
  JOURNAL   In Vitro Cell Dev Biol Anim 55 (4), 226-236 (2019)
   PUBMED   30790128
  REMARK    GeneRIF: Deletion of the insulin promoter region revealed that
            nucleotide positions - 238 to - 144 (relative to the
            transcriptional start site) were needed for ER repression of the
            rat insulin II gene.
REFERENCE   4  (bases 1 to 464)
  AUTHORS   Gao,H., Ho,E. and Yallampalli,C.
  TITLE     Ghrelin doesn't limit insulin release in pregnant rats fed low
            protein diet
  JOURNAL   Front Biosci (Landmark Ed) 22 (9), 1523-1533 (2017)
   PUBMED   28410130
  REMARK    GeneRIF: Expression of ghrelin and ghrelin receptor and glucose
            stimulated insulin secretion (GSIS) of pancreatic islets.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 464)
  AUTHORS   Dayyani,F., Zurita,A.J., Nogueras-Gonzalez,G.M., Slack,R.,
            Millikan,R.E., Araujo,J.C., Gallick,G.E., Logothetis,C.J. and
            Corn,P.G.
  TITLE     The combination of serum insulin, osteopontin, and hepatocyte
            growth factor predicts time to castration-resistant progression in
            androgen dependent metastatic prostate cancer- an exploratory study
  JOURNAL   BMC Cancer 16 (1), 721 (2016)
   PUBMED   27599544
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 464)
  AUTHORS   Olansky,L., Welling,C., Giddings,S., Adler,S., Bourey,R., Dowse,G.,
            Serjeantson,S., Zimmet,P. and Permutt,M.A.
  TITLE     A variant insulin promoter in non-insulin-dependent diabetes
            mellitus
  JOURNAL   J Clin Invest 89 (5), 1596-1602 (1992)
   PUBMED   1569197
REFERENCE   7  (bases 1 to 464)
  AUTHORS   Lomedico,P., Rosenthal,N., Efstratidadis,A., Gilbert,W.,
            Kolodner,R. and Tizard,R.
  TITLE     The structure and evolution of the two nonallelic rat preproinsulin
            genes
  JOURNAL   Cell 18 (2), 545-558 (1979)
   PUBMED   498284
REFERENCE   8  (bases 1 to 464)
  AUTHORS   Chan,S.J., Noyes,B.E., Agarwal,K.L. and Steiner,D.F.
  TITLE     Construction and selection of recombinant plasmids containing
            full-length complementary DNAs corresponding to rat insulins I and
            II
  JOURNAL   Proc Natl Acad Sci U S A 76 (10), 5036-5040 (1979)
   PUBMED   388427
REFERENCE   9  (bases 1 to 464)
  AUTHORS   Tager,H., Given,B., Baldwin,D., Mako,M., Markese,J., Rubenstein,A.,
            Olefsky,J., Kobayashi,M., Kolterman,O. and Poucher,R.
  TITLE     A structurally abnormal insulin causing human diabetes
  JOURNAL   Nature 281 (5727), 122-125 (1979)
   PUBMED   381941
REFERENCE   10 (bases 1 to 464)
  AUTHORS   Schade,D.S. and Eaton,R.P.
  TITLE     Glucagon regulation of plasma ketone body concentration in human
            diabetes
  JOURNAL   J Clin Invest 56 (5), 1340-1344 (1975)
   PUBMED   1184755
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BP467629.1, BP485158.1 and BP472188.1.
            
            On Jun 3, 2010 this sequence version replaced NM_019130.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BP471674.1, BP485158.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN06680419,
                                           SAMN06680428 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-8                 BP467629.1         439-446             c
            9-442               BP485158.1         8-441
            443-464             BP472188.1         419-440
FEATURES             Location/Qualifiers
     source          1..464
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10116"
                     /chromosome="1"
                     /map="1q41"
     gene            1..464
                     /gene="Ins2"
                     /gene_synonym="CP-II"
                     /note="insulin 2"
                     /db_xref="GeneID:24506"
                     /db_xref="RGD:2916"
     exon            1..43
                     /gene="Ins2"
                     /gene_synonym="CP-II"
                     /inference="alignment:Splign:2.1.0"
     exon            44..244
                     /gene="Ins2"
                     /gene_synonym="CP-II"
                     /inference="alignment:Splign:2.1.0"
     CDS             58..390
                     /gene="Ins2"
                     /gene_synonym="CP-II"
                     /note="C-peptide"
                     /codon_start=1
                     /product="insulin-2 preproprotein"
                     /protein_id="NP_062003.1"
                     /db_xref="GeneID:24506"
                     /db_xref="RGD:2916"
                     /translation="MALWIRFLPLLALLILWEPRPAQAFVKQHLCGSHLVEALYLVCG
                     ERGFFYTPMSRREVEDPQVAQLELGGGPGAGDLQTLALEVARQKRGIVDQCCTSICSL
                     YQLENYCN"
     exon            245..450
                     /gene="Ins2"
                     /gene_synonym="CP-II"
                     /inference="alignment:Splign:2.1.0"
     regulatory      424..429
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ins2"
                     /gene_synonym="CP-II"
     polyA_site      450
                     /gene="Ins2"
                     /gene_synonym="CP-II"
ORIGIN      
        1 agccctaagt gaccagctac agtcggaaac catcagcaag caggtcattg ttccaacatg
       61 gccctgtgga tccgcttcct gcccctgctg gccctgctca tcctctggga gccccgccct
      121 gcccaggctt ttgtcaaaca gcacctttgt ggttctcact tggtggaagc tctctacctg
      181 gtgtgtgggg agcgtggatt cttctacaca cccatgtccc gccgcgaagt ggaggaccca
      241 caagtggcac aactggagct gggtggaggc ccgggggcag gtgaccttca gaccttggca
      301 ctggaggtgg cccggcagaa gcgcggcatc gtggatcagt gctgcaccag catctgctct
      361 ctctaccaac tggagaacta ctgcaactag gcccaccact accctgtcca cccctctgca
      421 atgaataaaa cctttgaaag agcactacaa aaaaaaaaaa aaaa
//
