LOCUS       NM_019129                463 bp    mRNA    linear   ROD 02-APR-2024
DEFINITION  Rattus norvegicus insulin 1 (Ins1), mRNA.
ACCESSION   NM_019129
VERSION     NM_019129.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 463)
  AUTHORS   Harvey,K.E., LaVigne,E.K., Dar,M.S., Salyer,A.E., Pratt,E.P.S.,
            Sample,P.A., Aryal,U.K., Gowher,H. and Hockerman,G.H.
  TITLE     RyR2/IRBIT regulates insulin gene transcript, insulin content, and
            secretion in the insulinoma cell line INS-1
  JOURNAL   Sci Rep 12 (1), 7713 (2022)
   PUBMED   35562179
  REMARK    GeneRIF: RyR2/IRBIT regulates insulin gene transcript, insulin
            content, and secretion in the insulinoma cell line INS-1.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 463)
  AUTHORS   Toyoshima,Y., Nakamura,K., Tokita,R., Teramoto,N., Sugihara,H.,
            Kato,H., Yamanouchi,K. and Minami,S.
  TITLE     Disruption of insulin receptor substrate-2 impairs growth but not
            insulin function in rats
  JOURNAL   J Biol Chem 295 (33), 11914-11927 (2020)
   PUBMED   32631952
  REMARK    GeneRIF: Disruption of insulin receptor substrate-2 impairs growth
            but not insulin function in rats.
REFERENCE   3  (bases 1 to 463)
  AUTHORS   Elabadlah,H., Hameed,R., D'Souza,C., Mohsin,S. and Adeghate,E.A.
  TITLE     Exogenous Ghrelin Increases Plasma Insulin Level in Diabetic Rats
  JOURNAL   Biomolecules 10 (4), 633 (2020)
   PUBMED   32325912
  REMARK    GeneRIF: Exogenous Ghrelin Increases Plasma Insulin Level in
            Diabetic Rats.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 463)
  AUTHORS   Billert,M., Sassek,M., Wojciechowicz,T., Jasaszwili,M.,
            Strowski,M.Z., Nowak,K.W. and Skrzypski,M.
  TITLE     Neuropeptide B stimulates insulin secretion and expression but not
            proliferation in rat insulin-producing INS-1E cells
  JOURNAL   Mol Med Rep 20 (2), 2030-2038 (2019)
   PUBMED   31257494
  REMARK    GeneRIF: NPB and NPBW1 mRNA was expressed in INS1E cells and rat
            pancreatic islets. In INS1E cells, NPB enhanced insulin 1 mRNA
            expression via an ERK1/2dependent mechanism
REFERENCE   5  (bases 1 to 463)
  AUTHORS   Nakane,T., Ido,A., Higuchi,T., Todaka,H., Morisawa,K., Nagamine,T.,
            Fukunaga,K., Sakamoto,S., Murao,K. and Sugiyama,Y.
  TITLE     Candidate plasticity gene 16 mediates suppression of insulin gene
            expression in rat insulinoma INS-1cells under glucotoxic conditions
  JOURNAL   Biochem Biophys Res Commun 512 (2), 189-195 (2019)
   PUBMED   30879761
  REMARK    GeneRIF: These results suggest that CPG16 plays a role in the
            suppression of insulin gene expression in pancreatic beta-cells
            under glucotoxic conditions.
REFERENCE   6  (bases 1 to 463)
  AUTHORS   Olansky,L., Welling,C., Giddings,S., Adler,S., Bourey,R., Dowse,G.,
            Serjeantson,S., Zimmet,P. and Permutt,M.A.
  TITLE     A variant insulin promoter in non-insulin-dependent diabetes
            mellitus
  JOURNAL   J Clin Invest 89 (5), 1596-1602 (1992)
   PUBMED   1569197
REFERENCE   7  (bases 1 to 463)
  AUTHORS   Lomedico,P., Rosenthal,N., Efstratidadis,A., Gilbert,W.,
            Kolodner,R. and Tizard,R.
  TITLE     The structure and evolution of the two nonallelic rat preproinsulin
            genes
  JOURNAL   Cell 18 (2), 545-558 (1979)
   PUBMED   498284
REFERENCE   8  (bases 1 to 463)
  AUTHORS   Cordell,B., Bell,G., Tischer,E., DeNoto,F.M., Ullrich,A.,
            Pictet,R., Rutter,W.J. and Goodman,H.M.
  TITLE     Isolation and characterization of a cloned rat insulin gene
  JOURNAL   Cell 18 (2), 533-543 (1979)
   PUBMED   498283
REFERENCE   9  (bases 1 to 463)
  AUTHORS   Villa-Komaroff,L., Efstratiadis,A., Broome,S., Lomedico,P.,
            Tizard,R., Naber,S.P., Chick,W.L. and Gilbert,W.
  TITLE     A bacterial clone synthesizing proinsulin
  JOURNAL   Proc Natl Acad Sci U S A 75 (8), 3727-3731 (1978)
   PUBMED   358198
REFERENCE   10 (bases 1 to 463)
  AUTHORS   Ullrich,A., Shine,J., Chirgwin,J., Pictet,R., Tischer,E.,
            Rutter,W.J. and Goodman,H.M.
  TITLE     Rat insulin genes: construction of plasmids containing the coding
            sequences
  JOURNAL   Science 196 (4296), 1313-1319 (1977)
   PUBMED   325648
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BP502578.1 and BP465191.1.
            
            On Jun 3, 2010 this sequence version replaced NM_019129.2.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: FQ231224.1, BP502578.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN12840107 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-334               BP502578.1         21-354
            335-463             BP465191.1         287-415
FEATURES             Location/Qualifiers
     source          1..463
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10116"
                     /chromosome="1"
                     /map="1q55"
     gene            1..463
                     /gene="Ins1"
                     /note="insulin 1"
                     /db_xref="GeneID:24505"
                     /db_xref="RGD:2915"
     exon            1..43
                     /gene="Ins1"
                     /inference="alignment:Splign:2.1.0"
     exon            44..449
                     /gene="Ins1"
                     /inference="alignment:Splign:2.1.0"
     CDS             58..390
                     /gene="Ins1"
                     /codon_start=1
                     /product="insulin-1 preproprotein"
                     /protein_id="NP_062002.1"
                     /db_xref="GeneID:24505"
                     /db_xref="RGD:2915"
                     /translation="MALWMRFLPLLALLVLWEPKPAQAFVKQHLCGPHLVEALYLVCG
                     ERGFFYTPKSRREVEDPQVPQLELGGGPEAGDLQTLALEVARQKRGIVDQCCTSICSL
                     YQLENYCN"
     regulatory      423..428
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ins1"
     polyA_site      449
                     /gene="Ins1"
ORIGIN      
        1 aaccctaagt gaccagctac aatcatagac catcagcaag caggtcattg ttccaacatg
       61 gccctgtgga tgcgcttcct gcccctgctg gccctgctcg tcctctggga gcccaagcct
      121 gcccaggctt ttgtcaaaca gcacctttgt ggtcctcacc tggtggaggc tctgtacctg
      181 gtgtgtgggg aacgtggttt cttctacaca cccaagtccc gtcgtgaagt ggaggacccg
      241 caagtgccac aactggagct gggtggaggc ccggaggccg gggatcttca gaccttggca
      301 ctggaggttg cccggcagaa gcgtggcatt gtggatcagt gctgcaccag catctgctcc
      361 ctctaccaac tggagaacta ctgcaactga gtccaccact ccccgcccac ccctctgcaa
      421 tgaataaagc ctttgaatga gcaccaaaaa aaaaaaaaaa aaa
//
