LOCUS       NM_133409               1770 bp    mRNA    linear   ROD 08-DEC-2024
DEFINITION  Rattus norvegicus integrin-linked kinase (Ilk), mRNA.
ACCESSION   NM_133409
VERSION     NM_133409.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 1770)
  AUTHORS   Yan,G., Sun,R., Chen,Z., Pan,X., Sheng,Z. and Tang,C.
  TITLE     PTBP1 Targets ILK to Regulate the Hypoxia-Induced Phenotypic
            Transformation of Pulmonary Artery Smooth Muscle Cells
  JOURNAL   Drug Des Devel Ther 15, 2025-2033 (2021)
   PUBMED   34012255
  REMARK    GeneRIF: PTBP1 Targets ILK to Regulate the Hypoxia-Induced
            Phenotypic Transformation of Pulmonary Artery Smooth Muscle Cells.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1770)
  AUTHORS   Hou,J., Liu,B., Zhu,B., Wang,D., Qiao,Y., Luo,E., Nawabi,A.Q.,
            Yan,G. and Tang,C.
  TITLE     Role of integrin-linked kinase in the hypoxia-induced phenotypic
            transition of pulmonary artery smooth muscle cells: Implications
            for hypoxic pulmonary hypertension
  JOURNAL   Exp Cell Res 382 (2), 111476 (2019)
   PUBMED   31255599
  REMARK    GeneRIF: ILK initiates the phenotypic transition of pulmonary
            artery smooth muscle cells.
REFERENCE   3  (bases 1 to 1770)
  AUTHORS   Li,M., Zhou,H., Di,J., Yang,M. and Jia,F.
  TITLE     ILK participates in renal interstitial fibrosis by altering the
            phenotype of renal tubular epithelial cells via TGF-beta1/smad
            pathway
  JOURNAL   Eur Rev Med Pharmacol Sci 23 (1), 289-296 (2019)
   PUBMED   30657569
  REMARK    GeneRIF: Accompanied by the TGF-beta1-induced phenotype change in
            rat kidney epithelial cell line NRK-52E, both mRNA and protein
            levels of ILK were upregulated. Overexpression of ILK remarkably
            stimulated the phenotype change in NRK-52E cells. ILK is
            upregulated during renal fibrosis. ILK participates in the
            development of renal fibrosis by altering phenotypes of renal
            tubular epithelial cells via a TGF-beta1/smad pathway.
REFERENCE   4  (bases 1 to 1770)
  AUTHORS   Li,G., Li,Y.Y., Sun,J.E., Lin,W.H. and Zhou,R.X.
  TITLE     ILK-PI3K/AKT pathway participates in cutaneous wound contraction by
            regulating fibroblast migration and differentiation to
            myofibroblast
  JOURNAL   Lab Invest 96 (7), 741-751 (2016)
   PUBMED   27111285
  REMARK    GeneRIF: Investigated the role of ILK in cutaneous wound
            contraction. We found that ILK was involved in cutaneous wound
            healing, and ILK and PI3K/AKT inhibitors inhibited wound
            contraction and re-epithelialization, consequently delaying wound
            healing in vivo.
REFERENCE   5  (bases 1 to 1770)
  AUTHORS   Moraes,J.A., Frony,A.C., Dias,A.M., Renovato-Martins,M.,
            Rodrigues,G., Marcinkiewicz,C., Assreuy,J. and Barja-Fidalgo,C.
  TITLE     Alpha1beta1 and integrin-linked kinase interact and modulate
            angiotensin II effects in vascular smooth muscle cells
  JOURNAL   Atherosclerosis 243 (2), 477-485 (2015)
   PUBMED   26520903
  REMARK    GeneRIF: Alpha1beta1 and integrin-linked kinase interact and
            modulate angiotensin II effects on vascular smooth muscle cell
            proliferation/migration.
REFERENCE   6  (bases 1 to 1770)
  AUTHORS   Mulholland,D.J., Dedhar,S. and Vogl,A.W.
  TITLE     Rat seminiferous epithelium contains a unique junction (Ectoplasmic
            specialization) with signaling properties both of cell/cell and
            cell/matrix junctions
  JOURNAL   Biol Reprod 64 (1), 396-407 (2001)
   PUBMED   11133699
REFERENCE   7  (bases 1 to 1770)
  AUTHORS   Janji,B., Melchior,C., Vallar,L. and Kieffer,N.
  TITLE     Cloning of an isoform of integrin-linked kinase (ILK) that is
            upregulated in HT-144 melanoma cells following TGF-beta1
            stimulation
  JOURNAL   Oncogene 19 (27), 3069-3077 (2000)
   PUBMED   10871859
REFERENCE   8  (bases 1 to 1770)
  AUTHORS   Lynch,D.K., Ellis,C.A., Edwards,P.A. and Hiles,I.D.
  TITLE     Integrin-linked kinase regulates phosphorylation of serine 473 of
            protein kinase B by an indirect mechanism
  JOURNAL   Oncogene 18 (56), 8024-8032 (1999)
   PUBMED   10637513
REFERENCE   9  (bases 1 to 1770)
  AUTHORS   Li,F., Liu,J., Mayne,R. and Wu,C.
  TITLE     Identification and characterization of a mouse protein kinase that
            is highly homologous to human integrin-linked kinase
  JOURNAL   Biochim Biophys Acta 1358 (3), 215-220 (1997)
   PUBMED   9366252
REFERENCE   10 (bases 1 to 1770)
  AUTHORS   Hannigan,G.E., Leung-Hagesteijn,C., Fitz-Gibbon,L., Coppolino,M.G.,
            Radeva,G., Filmus,J., Bell,J.C. and Dedhar,S.
  TITLE     Regulation of cell adhesion and anchorage-dependent growth by a new
            beta 1-integrin-linked protein kinase
  JOURNAL   Nature 379 (6560), 91-96 (1996)
   PUBMED   8538749
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from BC062406.1.
            
            On Dec 20, 2003 this sequence version replaced NM_133409.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC062406.1, SRR8487227.44050.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMD00132261, SAMD00132262
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..1770
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10116"
                     /chromosome="1"
                     /map="1q32"
     gene            1..1770
                     /gene="Ilk"
                     /gene_synonym="ILK-1; ILK-2; p59ILK"
                     /note="integrin-linked kinase"
                     /db_xref="GeneID:170922"
                     /db_xref="RGD:620063"
     exon            1..15
                     /gene="Ilk"
                     /gene_synonym="ILK-1; ILK-2; p59ILK"
                     /inference="alignment:Splign:2.1.0"
     exon            16..152
                     /gene="Ilk"
                     /gene_synonym="ILK-1; ILK-2; p59ILK"
                     /inference="alignment:Splign:2.1.0"
     CDS             64..1422
                     /gene="Ilk"
                     /gene_synonym="ILK-1; ILK-2; p59ILK"
                     /EC_number="2.7.11.1"
                     /note="integrin-linked protein kinase;
                     beta-integrin-linked kinase; 59 kDa
                     serine/threonine-protein kinase; inactive integrin-linked
                     kinase"
                     /codon_start=1
                     /product="scaffold protein ILK"
                     /protein_id="NP_596900.1"
                     /db_xref="GeneID:170922"
                     /db_xref="RGD:620063"
                     /translation="MDDIFTQCREGNAVAVRLWLDNTENDLNQGDDHGFSPLHWACRE
                     GRSAVVEMLIMRGARINVMNRGDDTPLHLAASHGHRDIVQKLLQYKADINAVNEHGNV
                     PLHYACFWGQDQVAEDLVANGALVSICNKYGEMPVDKAKAPLRELLRERAEKMGQNLN
                     RIPYKDTFWKGTTRTRPRNGTLNKHSGIDFKQLNFLAKLNENHSGELWKGRWQGNDIV
                     VKVLKVRDWSTRKSRDFNEECPRLRIFSHPNVLPVLGACQAPPAPHPTLITHWMPYGS
                     LYNVLHEGTNFVVDQSQAVKFALDMARGMAFLHTLEPLIPRHALNSRSVMIDEDMTAR
                     ISMADVKFSFQCPGRMYAPAWVAPEALQKKPEDTNRRSADMWSFAVLLWELVTREVPF
                     ADLSNMEIGMKVALEGLRPTIPPGISPHVCKLMKICMNEDPAKRPKFDMIVPILEKMQ
                     DK"
     exon            153..318
                     /gene="Ilk"
                     /gene_synonym="ILK-1; ILK-2; p59ILK"
                     /inference="alignment:Splign:2.1.0"
     exon            319..414
                     /gene="Ilk"
                     /gene_synonym="ILK-1; ILK-2; p59ILK"
                     /inference="alignment:Splign:2.1.0"
     exon            415..511
                     /gene="Ilk"
                     /gene_synonym="ILK-1; ILK-2; p59ILK"
                     /inference="alignment:Splign:2.1.0"
     exon            512..595
                     /gene="Ilk"
                     /gene_synonym="ILK-1; ILK-2; p59ILK"
                     /inference="alignment:Splign:2.1.0"
     exon            596..681
                     /gene="Ilk"
                     /gene_synonym="ILK-1; ILK-2; p59ILK"
                     /inference="alignment:Splign:2.1.0"
     exon            682..791
                     /gene="Ilk"
                     /gene_synonym="ILK-1; ILK-2; p59ILK"
                     /inference="alignment:Splign:2.1.0"
     exon            792..919
                     /gene="Ilk"
                     /gene_synonym="ILK-1; ILK-2; p59ILK"
                     /inference="alignment:Splign:2.1.0"
     exon            920..1041
                     /gene="Ilk"
                     /gene_synonym="ILK-1; ILK-2; p59ILK"
                     /inference="alignment:Splign:2.1.0"
     exon            1042..1141
                     /gene="Ilk"
                     /gene_synonym="ILK-1; ILK-2; p59ILK"
                     /inference="alignment:Splign:2.1.0"
     exon            1142..1272
                     /gene="Ilk"
                     /gene_synonym="ILK-1; ILK-2; p59ILK"
                     /inference="alignment:Splign:2.1.0"
     exon            1273..1686
                     /gene="Ilk"
                     /gene_synonym="ILK-1; ILK-2; p59ILK"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gcgacccgag tcccgttccc cgagacttcc ccagtcccgg ggtctctgtg ccgggacgct
       61 gctatggacg acattttcac tcagtgccgg gagggcaacg cggtggcggt gcgcttgtgg
      121 ctggacaaca cagagaacga cctaaatcag ggggatgatc atggcttctc ccctttgcac
      181 tgggcctgcc gagaaggccg atctgcagtg gttgaaatgc tgatcatgcg gggagcacgg
      241 atcaatgtga tgaatcgtgg ggatgatacc cccctgcact tggcagctag tcacggacac
      301 cgtgacattg tacagaagct gttgcaatac aaggctgaca tcaatgcagt gaatgaacat
      361 gggaatgtgc cccttcacta tgcatgtttc tggggccaag accaggtggc agaggaccta
      421 gtagctaacg gggctcttgt gagcatctgt aacaagtatg gagagatgcc tgtggacaaa
      481 gccaaggcac cccttcgaga gcttctccga gaacgggcag agaagatggg ccagaatctt
      541 aatcgtattc catacaagga cacattctgg aaggggacca ctcgcacaag gccccgaaat
      601 gggaccctga acaaacactc tggtattgac ttcaaacagc tcaactttct ggcaaagctc
      661 aacgagaatc attctggaga gctatggaaa ggccgctggc agggcaatga cattgttgtg
      721 aaggtgctga aggttcgaga ctggagtaca aggaaaagca gggacttcaa tgaggaatgt
      781 ccccggctca ggattttctc tcatcctaac gtgcttccgg tactaggtgc ttgccaggct
      841 cccccagctc cccacccaac cctcatcaca cactggatgc catatggatc tctctacaat
      901 gttctacatg aaggcaccaa tttcgttgtg gaccagagcc aagctgtaaa gtttgctttg
      961 gacatggcaa gaggcatggc ttttcttcat acactagagc ctctcatacc tcggcacgca
     1021 ctcaatagcc gtagtgtaat gatcgatgaa gatatgactg cccgaatcag catggctgat
     1081 gtaaagtttt ctttccagtg ccctgggcgc atgtatgcac ctgcctgggt ggcccctgaa
     1141 gccctgcaaa agaagcctga agatacaaac agacgctcag cagacatgtg gagttttgcc
     1201 gtgcttctgt gggaactggt gacacgagag gtgccctttg ctgacctttc taatatggag
     1261 attggaatga aggtggcact ggaaggcctt cggcctacta tcccaccagg tatttccccc
     1321 cacgtgtgta agctcatgaa gatttgcatg aatgaagacc ctgcaaagcg acccaagttt
     1381 gacatgattg tacctatctt ggagaagatg caggacaagt aggacttgaa ggtccctgcc
     1441 caaacttcag aagtatcagc atatggctgg gggaatgcac ttccccgaag ctgaagatct
     1501 ctggttgcct cccctgtctc taatcatggt actaccccag ttatggggct tgttccctgc
     1561 tcccatccct accactgtag ccccagagag ggctgggctc agagctttgt cacttgccac
     1621 ttgatgtctc ccaacatggg agggatcagc cccgcctgtc acaataaagt ttattatgaa
     1681 aacaggaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
     1741 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
//
