LOCUS       NM_053638               2465 bp    mRNA    linear   ROD 09-APR-2024
DEFINITION  Rattus norvegicus isocitrate dehydrogenase (NAD(+)) 3 catalytic
            subunit alpha (Idh3a), mRNA; nuclear gene for mitochondrial
            product.
ACCESSION   NM_053638
VERSION     NM_053638.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 2465)
  AUTHORS   Ma,T., Peng,Y., Huang,W. and Ding,J.
  TITLE     Molecular mechanism of the allosteric regulation of the alphagamma
            heterodimer of human NAD-dependent isocitrate dehydrogenase
  JOURNAL   Sci Rep 7, 40921 (2017)
   PUBMED   28098230
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2465)
  AUTHORS   de Mateo,S., Castillo,J., Estanyol,J.M., Ballesca,J.L. and Oliva,R.
  TITLE     Proteomic characterization of the human sperm nucleus
  JOURNAL   Proteomics 11 (13), 2714-2726 (2011)
   PUBMED   21630459
REFERENCE   3  (bases 1 to 2465)
  AUTHORS   Werner,H.B., Kuhlmann,K., Shen,S., Uecker,M., Schardt,A.,
            Dimova,K., Orfaniotou,F., Dhaunchak,A., Brinkmann,B.G., Mobius,W.,
            Guarente,L., Casaccia-Bonnefil,P., Jahn,O. and Nave,K.A.
  TITLE     Proteolipid protein is required for transport of sirtuin 2 into CNS
            myelin
  JOURNAL   J Neurosci 27 (29), 7717-7730 (2007)
   PUBMED   17634366
REFERENCE   4  (bases 1 to 2465)
  AUTHORS   Soundar,S., Park,J.H., Huh,T.L. and Colman,R.F.
  TITLE     Evaluation by mutagenesis of the importance of 3 arginines in
            alpha, beta, and gamma subunits of human NAD-dependent isocitrate
            dehydrogenase
  JOURNAL   J Biol Chem 278 (52), 52146-52153 (2003)
   PUBMED   14555658
REFERENCE   5  (bases 1 to 2465)
  AUTHORS   Mootha,V.K., Bunkenborg,J., Olsen,J.V., Hjerrild,M.,
            Wisniewski,J.R., Stahl,E., Bolouri,M.S., Ray,H.N., Sihag,S.,
            Kamal,M., Patterson,N., Lander,E.S. and Mann,M.
  TITLE     Integrated analysis of protein composition, tissue diversity, and
            gene regulation in mouse mitochondria
  JOURNAL   Cell 115 (5), 629-640 (2003)
   PUBMED   14651853
REFERENCE   6  (bases 1 to 2465)
  AUTHORS   Shinohara,Y., Daikoku,T., Kajimoto,K., Shima,A., Yamazaki,N. and
            Terada,H.
  TITLE     Expression of NAD(+)-dependent isocitrate dehydrogenase in brown
            adipose tissue
  JOURNAL   Biochem Biophys Res Commun 281 (3), 634-638 (2001)
   PUBMED   11237704
REFERENCE   7  (bases 1 to 2465)
  AUTHORS   Huang,Y.C. and Colman,R.F.
  TITLE     Subunit location and sequences of the cysteinyl peptides of pig
            heart NAD-dependent isocitrate dehydrogenase
  JOURNAL   Biochemistry 29 (36), 8266-8273 (1990)
   PUBMED   2252888
REFERENCE   8  (bases 1 to 2465)
  AUTHORS   Lai,J.C. and Clark,J.B.
  TITLE     Preparation and properties of mitochondria derived from
            synaptosomes
  JOURNAL   Biochem J 154 (2), 423-432 (1976)
   PUBMED   938457
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000008.1.
            
            On Nov 24, 2020 this sequence version replaced NM_053638.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AB047541.1, SRR26360172.1322415.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760383, SAMEA5760386
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            CDS uses downstream in-frame AUG   :: upstream AUG and CDS
                                                  extension is not conserved
            gene product(s) localized to mito. :: inferred from homology
            RefSeq Select criteria             :: based on conservation,
                                                  expression, longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-98                JAXUCZ010000008.1  63867882-63867979
            99-161              JAXUCZ010000008.1  63873006-63873068
            162-245             JAXUCZ010000008.1  63875686-63875769
            246-360             JAXUCZ010000008.1  63877736-63877850
            361-548             JAXUCZ010000008.1  63878670-63878857
            549-682             JAXUCZ010000008.1  63879498-63879631
            683-785             JAXUCZ010000008.1  63881168-63881270
            786-850             JAXUCZ010000008.1  63881369-63881433
            851-935             JAXUCZ010000008.1  63882352-63882436
            936-1088            JAXUCZ010000008.1  63883799-63883951
            1089-2465           JAXUCZ010000008.1  63885847-63887223
FEATURES             Location/Qualifiers
     source          1..2465
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="8"
                     /map="8q24"
     gene            1..2465
                     /gene="Idh3a"
                     /note="isocitrate dehydrogenase (NAD(+)) 3 catalytic
                     subunit alpha"
                     /db_xref="GeneID:114096"
                     /db_xref="RGD:70889"
     exon            1..98
                     /gene="Idh3a"
                     /inference="alignment:Splign:2.1.0"
     CDS             72..1172
                     /gene="Idh3a"
                     /EC_number="1.1.1.41"
                     /note="isocitrate dehydrogenase [NAD] subunit alpha,
                     mitochondrial; NAD(+)-specific ICDH subunit alpha;
                     isocitric dehydrogenase subunit alpha; isocitrate
                     dehydrogenase (NAD(+)) 3 alpha; isocitrate dehydrogenase 3
                     (NAD+) alpha"
                     /codon_start=1
                     /product="isocitrate dehydrogenase [NAD] subunit alpha,
                     mitochondrial precursor"
                     /protein_id="NP_446090.2"
                     /db_xref="GeneID:114096"
                     /db_xref="RGD:70889"
                     /translation="MAGSAWVSKVSRLLGAFHNTKQVTRGFAGGVQTVTLIPGDGIGP
                     EISASVMKIFDAAKAPIQWEERNVTAIQGPGGKWMIPPEAKESMDKNKMGLKGPLKTP
                     IAAGHPSMNLLLRKTFDLYANVRPCVSIEGYKTPYTDVNIVTIRENTEGEYSGIEHVI
                     VDGVVQSIKLITEGASKRIAEFAFEYARNNHRSNVTAVHKANIMRMSDGLFLQKCREV
                     AENCKDIKFNEMYLDTVCLNMVQDPSQFDVLVMPNLYGDILSDLCAGLIGGLGVTPSG
                     NIGANGVAIFESVHGTAPDIAGKDMANPTALLLSAVMMLRHMGLFDHAAKIEAACFAT
                     IKDGKSLTKDLGGNSKCSDFTEEICRRVKDLD"
     exon            99..161
                     /gene="Idh3a"
                     /inference="alignment:Splign:2.1.0"
     exon            162..245
                     /gene="Idh3a"
                     /inference="alignment:Splign:2.1.0"
     exon            246..360
                     /gene="Idh3a"
                     /inference="alignment:Splign:2.1.0"
     exon            361..548
                     /gene="Idh3a"
                     /inference="alignment:Splign:2.1.0"
     exon            549..682
                     /gene="Idh3a"
                     /inference="alignment:Splign:2.1.0"
     exon            683..785
                     /gene="Idh3a"
                     /inference="alignment:Splign:2.1.0"
     exon            786..850
                     /gene="Idh3a"
                     /inference="alignment:Splign:2.1.0"
     exon            851..935
                     /gene="Idh3a"
                     /inference="alignment:Splign:2.1.0"
     exon            936..1088
                     /gene="Idh3a"
                     /inference="alignment:Splign:2.1.0"
     exon            1089..2465
                     /gene="Idh3a"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 ggactggggc gccagctacg cgcatgcgcc ctggtgttgc cgctgtcgcc gcggtgaggg
       61 aagtggacgc gatggccggg tccgcgtggg tgtccaaggt ctctcggctg ctgggtgcct
      121 tccacaacac aaaacaggtg acaagaggtt ttgctggtgg cgttcagaca gtaactctaa
      181 ttcctggaga tggaattggc ccagaaattt cagcctcagt tatgaagatt tttgatgccg
      241 ccaaagcacc tattcagtgg gaggagcgga acgtcacagc gattcaagga ccaggaggaa
      301 agtggatgat ccctccagaa gccaaagagt ccatggataa gaacaagatg ggcttgaaag
      361 gcccactgaa gaccccaata gcagctggcc atccatctat gaatctgttg cttcgtaaga
      421 catttgatct ttatgccaat gtccggccat gtgtctcaat tgaaggttac aaaacccctt
      481 acacagatgt aaatattgtc accatccgag agaacacaga aggagaatac agtggaattg
      541 agcatgtgat cgtcgatggg gttgtgcaga gcatcaagct catcactgaa ggagcaagca
      601 agcgcattgc cgagtttgcc tttgagtacg cgaggaacaa ccacaggagc aatgtcacag
      661 ctgtgcacaa ggccaacatc atgaggatgt cagatgggct ctttctgcaa aaatgcaggg
      721 aagttgcaga gaactgtaaa gacattaagt ttaatgagat gtacctcgac actgtatgtt
      781 tgaatatggt acaagacccg tcccagttcg atgttcttgt catgccaaat ttatatggag
      841 acatccttag tgatctgtgt gcaggactga ttggaggtct tggtgtgact ccaagtggca
      901 atattggagc caacggtgtt gccatctttg aatcggttca cgggacagcc ccggacattg
      961 caggcaagga catggccaac cccacggccc tgctgctcag tgctgtgatg atgctgcgtc
     1021 acatgggcct ctttgaccac gcagcaaaaa tcgaggctgc gtgttttgct acaattaagg
     1081 atgggaagag cttaacaaaa gatctgggag gcaactcgaa gtgctctgac ttcacagaag
     1141 aaatctgtcg tagagtaaaa gacttagatt agcacttctg ctggggattt gctgcagtcc
     1201 gtccgtcact ccagggggat gccctgtaat cctccttgag agcgcccacc attggtttgc
     1261 ttgcttcgtg acagagcaca tttcaaatct ggccttttcc taacaaaacc cttgcaatgg
     1321 atgcacacaa tggccctagg cctttgtgca aaggattttc ccaagtgctt gtatttattg
     1381 tctgtctggt aaacctgttt tgtaaactat aagtgaactg tatcatttat cattgttaac
     1441 ccattttaca cctcaggcaa aataattttt cctcaactgt aaatattctg atacagaatt
     1501 aagagaagat atttaacttt ttaacaaaag ccctggattt ttggtttatg caaaacagct
     1561 gggagtaaaa cagggtttca acaatcgcac aagataatgt tattctaaaa atagtgggca
     1621 caaaagaaat ttactgggaa agttcacagc aaaaatgata tatttcttaa aaatatggaa
     1681 gtaaagatat ttgtcctata catgaattac tactaataaa aatgtaagct ccaagaaacc
     1741 cataacaacg gaagtaattt tattaatata tagataatcc ttgtcaaagc cccagatgct
     1801 ctgtgtattt gattattttg ttacttgaga tttactattt tggggggaag agctctcaga
     1861 cagggagatc actcctcttc actagactaa tcatcagcag ttgtcagtca gaggagccag
     1921 ccactgcaga ttccctccca catgaccaca ctccagccag ggctgcaggt gaccctggaa
     1981 aggcctggct gccccttcac ttcctaaagc aagcgtcact accctatgcc tcagtagtac
     2041 ccgttaaaga cttaattgca gaggtggggt ttactcaatc caaactgaca caaatcaggc
     2101 caagctgggg cctgtgtagc cagcttctga ccatcagttt ctgcccctcc ctctcccacc
     2161 cctgtccctt cccacagcct ctgcaccaca gcatgaaaat acccttcggc ccataccaaa
     2221 gccaagctgt tttcccttag cccttgtact agtttgcaaa cccatggctt tgcataatgt
     2281 accctggcca caggggattt cttaacaaca gatacccatg tctgggtcat tttttaaagc
     2341 ttttatttgg actcacagtc gtctctgtat tttactttaa aaccgctgct ttccctgtct
     2401 tactggattg ttctggttac cagtggcttt gggtccacag taataaagaa cttaagaact
     2461 gtctg
//
