LOCUS       NP_579844                284 aa            linear   ROD 03-APR-2024
DEFINITION  protein-S-isoprenylcysteine O-methyltransferase [Rattus
            norvegicus].
ACCESSION   NP_579844 XP_342989
VERSION     NP_579844.2
DBSOURCE    REFSEQ: accession NM_133310.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (residues 1 to 284)
  AUTHORS   Ibrahim,M.X., Sayin,V.I., Akula,M.K., Liu,M., Fong,L.G., Young,S.G.
            and Bergo,M.O.
  TITLE     Targeting isoprenylcysteine methylation ameliorates disease in a
            mouse model of progeria
  JOURNAL   Science 340 (6138), 1330-1333 (2013)
   PUBMED   23686339
REFERENCE   2  (residues 1 to 284)
  AUTHORS   Jayaram,B., Syed,I., Singh,A., Subasinghe,W., Kyathanahalli,C.N.
            and Kowluru,A.
  TITLE     Isoprenylcysteine carboxyl methyltransferase facilitates
            glucose-induced Rac1 activation, ROS generation and insulin
            secretion in INS 832/13 beta-cells
  JOURNAL   Islets 3 (2), 48-57 (2011)
   PUBMED   21346419
  REMARK    GeneRIF: data suggest that ICMT regulates glucose-induced Rac1
            activation, generation of reactive oxygen species and insulin
            secretion in pancreatic beta-cells
REFERENCE   3  (residues 1 to 284)
  AUTHORS   Baron,R.A. and Casey,P.J.
  TITLE     Analysis of the kinetic mechanism of recombinant human
            isoprenylcysteine carboxylmethyltransferase (Icmt)
  JOURNAL   BMC Biochem 5, 19 (2004)
   PUBMED   15625008
  REMARK    Publication Status: Online-Only
REFERENCE   4  (residues 1 to 284)
  AUTHORS   Bergo,M.O., Gavino,B.J., Hong,C., Beigneux,A.P., McMahon,M.,
            Casey,P.J. and Young,S.G.
  TITLE     Inactivation of Icmt inhibits transformation by oncogenic K-Ras and
            B-Raf
  JOURNAL   J Clin Invest 113 (4), 539-550 (2004)
   PUBMED   14966563
REFERENCE   5  (residues 1 to 284)
  AUTHORS   Kramer,K., Harrington,E.O., Lu,Q., Bellas,R., Newton,J.,
            Sheahan,K.L. and Rounds,S.
  TITLE     Isoprenylcysteine carboxyl methyltransferase activity modulates
            endothelial cell apoptosis
  JOURNAL   Mol Biol Cell 14 (3), 848-857 (2003)
   PUBMED   12631708
  REMARK    GeneRIF: findings suggest that inhibition of
            isoprenylcysteine-O-carboxyl methyltransferase (ICMT) causes
            endothelial cell apoptosis by attenuation of Ras GTPase methylation
            and activation and its downstream antiapoptotic signaling pathway.
REFERENCE   6  (residues 1 to 284)
  AUTHORS   Bergo,M.O., Leung,G.K., Ambroziak,P., Otto,J.C., Casey,P.J.,
            Gomes,A.Q., Seabra,M.C. and Young,S.G.
  TITLE     Isoprenylcysteine carboxyl methyltransferase deficiency in mice
  JOURNAL   J Biol Chem 276 (8), 5841-5845 (2001)
   PUBMED   11121396
REFERENCE   7  (residues 1 to 284)
  AUTHORS   Bergo,M.O., Leung,G.K., Ambroziak,P., Otto,J.C., Casey,P.J. and
            Young,S.G.
  TITLE     Targeted inactivation of the isoprenylcysteine carboxyl
            methyltransferase gene causes mislocalization of K-Ras in mammalian
            cells
  JOURNAL   J Biol Chem 275 (23), 17605-17610 (2000)
   PUBMED   10747846
REFERENCE   8  (residues 1 to 284)
  AUTHORS   Desrosiers,R.R., Nguyen,Q.T. and Beliveau,R.
  TITLE     The carboxyl methyltransferase modifying G proteins is a
            metalloenzyme
  JOURNAL   Biochem Biophys Res Commun 261 (3), 790-797 (1999)
   PUBMED   10441503
REFERENCE   9  (residues 1 to 284)
  AUTHORS   Dai,Q., Choy,E., Chiu,V., Romano,J., Slivka,S.R., Steitz,S.A.,
            Michaelis,S. and Philips,M.R.
  TITLE     Mammalian prenylcysteine carboxyl methyltransferase is in the
            endoplasmic reticulum
  JOURNAL   J Biol Chem 273 (24), 15030-15034 (1998)
   PUBMED   9614111
REFERENCE   10 (residues 1 to 284)
  AUTHORS   Boivin,D., Lin,W. and Beliveau,R.
  TITLE     Essential arginine residues in isoprenylcysteine protein carboxyl
            methyltransferase
  JOURNAL   Biochem Cell Biol 75 (1), 63-69 (1997)
   PUBMED   9192075
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            CB616023.1 and BC083923.1.
            
            On Oct 11, 2007 this sequence version replaced NP_579844.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF075595.1, EV765737.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMD00132261, SAMD00132263
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..284
                     /organism="Rattus norvegicus"
                     /db_xref="taxon:10116"
                     /chromosome="5"
                     /map="5q36"
     Protein         1..284
                     /product="protein-S-isoprenylcysteine O-methyltransferase"
                     /EC_number="2.1.1.100"
                     /note="farnesyl cysteine carboxyl methyltransferase; PPMT;
                     pcCMT; prenylcysteine carboxyl methyltransferase;
                     prenylated protein carboxyl methyltransferase;
                     prenylcysteine carboxyl methlytransferase"
                     /calculated_mol_wt=31681
     Site            17..33
                     /site_type="transmembrane region"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9WVM4.2)"
     Site            42..59
                     /site_type="transmembrane region"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9WVM4.2)"
     Site            70..87
                     /site_type="transmembrane region"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9WVM4.2)"
     Site            93..112
                     /site_type="transmembrane region"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9WVM4.2)"
     Site            132..149
                     /site_type="transmembrane region"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9WVM4.2)"
     Site            155..174
                     /site_type="transmembrane region"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9WVM4.2)"
     Region          163..257
                     /region_name="ICMT"
                     /note="Isoprenylcysteine carboxyl methyltransferase (ICMT)
                     family; pfam04140"
                     /db_xref="CDD:427740"
     Site            213..228
                     /site_type="transmembrane region"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9WVM4.2)"
     Site            230..244
                     /site_type="transmembrane region"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9WVM4.2)"
     CDS             1..284
                     /gene="Icmt"
                     /gene_synonym="fcmt"
                     /coded_by="NM_133310.2:58..912"
                     /db_xref="GeneID:170818"
                     /db_xref="RGD:621618"
ORIGIN      
        1 magcaarvpp gsearlslat fllgasvlal plltraglqg rtglalyvag lnalllllyr
       61 ppryqiaira cflgfvfgcg vllsfsqssw nhfgwyvcsl slfhyseylv tavnnpksls
      121 ldsfllnhsl eytvaalssw ieftlenifw pelkqitwls aagllmvifg eclrkvamft
      181 agsnfnhvvq seksdthtlv tsgvyawcrh psyvgwfyws igtqvmlcnp icgvvyaltv
      241 wrffrdrtee eeislihffg eeyldykkrv ptglpfikgv kvgl
//
