LOCUS       NM_001398608            3598 bp    mRNA    linear   ROD 22-SEP-2024
DEFINITION  Rattus norvegicus isoleucyl-tRNA synthetase 2, mitochondrial
            (Iars2), mRNA; nuclear gene for mitochondrial product.
ACCESSION   NM_001398608 XM_039091496
VERSION     NM_001398608.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 3598)
  AUTHORS   Morgenstern,M., Peikert,C.D., Lubbert,P., Suppanz,I., Klemm,C.,
            Alka,O., Steiert,C., Naumenko,N., Schendzielorz,A., Melchionda,L.,
            Muhlhauser,W.W.D., Knapp,B., Busch,J.D., Stiller,S.B.,
            Dannenmaier,S., Lindau,C., Licheva,M., Eickhorst,C., Galbusera,R.,
            Zerbes,R.M., Ryan,M.T., Kraft,C., Kozjak-Pavlovic,V., Drepper,F.,
            Dennerlein,S., Oeljeklaus,S., Pfanner,N., Wiedemann,N. and
            Warscheid,B.
  TITLE     Quantitative high-confidence human mitochondrial proteome and its
            dynamics in cellular context
  JOURNAL   Cell Metab 33 (12), 2464-2483 (2021)
   PUBMED   34800366
REFERENCE   2  (bases 1 to 3598)
  AUTHORS   Florea,L., Di Francesco,V., Miller,J., Turner,R., Yao,A.,
            Harris,M., Walenz,B., Mobarry,C., Merkulov,G.V., Charlab,R.,
            Dew,I., Deng,Z., Istrail,S., Li,P. and Sutton,G.
  TITLE     Gene and alternative splicing annotation with AIR
  JOURNAL   Genome Res 15 (1), 54-66 (2005)
   PUBMED   15632090
REFERENCE   3  (bases 1 to 3598)
  AUTHORS   Mootha,V.K., Bunkenborg,J., Olsen,J.V., Hjerrild,M.,
            Wisniewski,J.R., Stahl,E., Bolouri,M.S., Ray,H.N., Sihag,S.,
            Kamal,M., Patterson,N., Lander,E.S. and Mann,M.
  TITLE     Integrated analysis of protein composition, tissue diversity, and
            gene regulation in mouse mitochondria
  JOURNAL   Cell 115 (5), 629-640 (2003)
   PUBMED   14651853
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000013.1.
            
            On Dec 20, 2021 this sequence version replaced XM_039091496.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR26643291.34654.1,
                                           SRR26643295.47848.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA5760383,
                                           SAMEA5760386 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: inferred from homology
            RefSeq Select criteria             :: based on single
                                                  protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-364               JAXUCZ010000013.1  99396705-99397068   c
            365-487             JAXUCZ010000013.1  99395342-99395464   c
            488-647             JAXUCZ010000013.1  99393088-99393247   c
            648-796             JAXUCZ010000013.1  99391898-99392046   c
            797-846             JAXUCZ010000013.1  99391445-99391494   c
            847-956             JAXUCZ010000013.1  99391255-99391364   c
            957-1047            JAXUCZ010000013.1  99391086-99391176   c
            1048-1163           JAXUCZ010000013.1  99390671-99390786   c
            1164-1333           JAXUCZ010000013.1  99388991-99389160   c
            1334-1424           JAXUCZ010000013.1  99387846-99387936   c
            1425-1576           JAXUCZ010000013.1  99387080-99387231   c
            1577-1737           JAXUCZ010000013.1  99385064-99385224   c
            1738-1840           JAXUCZ010000013.1  99377404-99377506   c
            1841-1934           JAXUCZ010000013.1  99376867-99376960   c
            1935-2043           JAXUCZ010000013.1  99373688-99373796   c
            2044-2146           JAXUCZ010000013.1  99372575-99372677   c
            2147-2272           JAXUCZ010000013.1  99371879-99372004   c
            2273-2404           JAXUCZ010000013.1  99371105-99371236   c
            2405-2511           JAXUCZ010000013.1  99367411-99367517   c
            2512-2657           JAXUCZ010000013.1  99366639-99366784   c
            2658-2848           JAXUCZ010000013.1  99365484-99365674   c
            2849-2993           JAXUCZ010000013.1  99364442-99364586   c
            2994-3598           JAXUCZ010000013.1  99363035-99363639   c
FEATURES             Location/Qualifiers
     source          1..3598
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="13"
                     /map="13q26"
     gene            1..3598
                     /gene="Iars2"
                     /gene_synonym="RGD1311857"
                     /note="isoleucyl-tRNA synthetase 2, mitochondrial"
                     /db_xref="GeneID:364070"
                     /db_xref="RGD:1311857"
     exon            1..364
                     /gene="Iars2"
                     /gene_synonym="RGD1311857"
                     /inference="alignment:Splign:2.1.0"
     CDS             98..3136
                     /gene="Iars2"
                     /gene_synonym="RGD1311857"
                     /EC_number="6.1.1.5"
                     /note="mitochondrial isoleucine tRNA synthetase;
                     isoleucyl-tRNA synthetase, mitochondrial; isoleucine-tRNA
                     synthetase 2, mitochondrial"
                     /codon_start=1
                     /product="isoleucine--tRNA ligase, mitochondrial"
                     /protein_id="NP_001385537.1"
                     /db_xref="GeneID:364070"
                     /db_xref="RGD:1311857"
                     /translation="MLWGLHPHGPGTAALTAAGSLWGPARFPSRLGCLGMTRRLVVRS
                     VAGADSPQSSPKSGRYRDTVLLPQTNFPMKLLGHQQSDMELEIQQKCGFSELYSWQRE
                     RKVKTEFCLHDGPPYANGDPHVGHALNKILKDIVNRFHMMRGSKVHFVPGWDCHGLPI
                     ETKVLSELGVNTQTLSAMEIREKARSFAQAAIEKQKSAFARWGVMADWSNCYYTFDPK
                     YEAKQLRVFQQMYEKGLVYRSYKPVYWSPSSRTALAEAELEYNPEHVSRSVYVRFPLL
                     KPPPTLESLTGASSPVSALVWTTQPWTVPANQAICYMPEAKYAVVKCSASGNFYILAE
                     DKISPVASALETTFDVTATFSGVDLEGGTCSHPLIPDKVSPLLPATHVTMAKGTGLVH
                     TAPAHGMEDYSVASQHSLPMDCLVDEEGMFTSAAGPELQNKAVLTEGTDVVIKMLQAT
                     KNLLKEESIVHSYPCDWRTKKPVLIRASKQWFVNIMDIKATAKESLKTVKFIPGAALN
                     SMMDMLDRRPYWCISRQRVWGVPIPVFHHKTKDEYLINSQTTEHIIKLVEQHGSDVWW
                     TLPAEKLLPEEVVAQAGGPGALEYVPGQDILDIWFDSGTSWSCVLQGTEQRADLYLEG
                     KDQLGGWFQSSLLTSVATRSKAPFRTVMVHGFTLGEKGEKMSKSLGNVIDPDTIISGG
                     KDHSKEPPYGADILRWWIAESNVFTEVTIGPSVLSAARDDISKLRNTLRFLLGNLAGF
                     NPETASVPVKNMYIIDQYMLHLIQDFATKVTDSYKQYDFGKVVRLLKAFYTRELSSFY
                     FSIVKDRLYCESETDPKRRSCQTALAEILDVLVRSFAPVLPHLAEEVFQHIPYVKEPK
                     SVFRTGWISTSSVWKKPGLEEAVESACAMRDSFLGSIPGKNAAEYDVIIVIEPGLLFE
                     IMEMLQAEETSSTSQLNELMMASQTTLLAQEPRERSADAIELTGTFVINLEGGDIREE
                     SSYKVIVVPTAREKCPRCWKHTAETADALCPRCAEVIGAK"
     exon            365..487
                     /gene="Iars2"
                     /gene_synonym="RGD1311857"
                     /inference="alignment:Splign:2.1.0"
     exon            488..647
                     /gene="Iars2"
                     /gene_synonym="RGD1311857"
                     /inference="alignment:Splign:2.1.0"
     exon            648..796
                     /gene="Iars2"
                     /gene_synonym="RGD1311857"
                     /inference="alignment:Splign:2.1.0"
     exon            797..846
                     /gene="Iars2"
                     /gene_synonym="RGD1311857"
                     /inference="alignment:Splign:2.1.0"
     exon            847..956
                     /gene="Iars2"
                     /gene_synonym="RGD1311857"
                     /inference="alignment:Splign:2.1.0"
     exon            957..1047
                     /gene="Iars2"
                     /gene_synonym="RGD1311857"
                     /inference="alignment:Splign:2.1.0"
     exon            1048..1163
                     /gene="Iars2"
                     /gene_synonym="RGD1311857"
                     /inference="alignment:Splign:2.1.0"
     exon            1164..1333
                     /gene="Iars2"
                     /gene_synonym="RGD1311857"
                     /inference="alignment:Splign:2.1.0"
     exon            1334..1424
                     /gene="Iars2"
                     /gene_synonym="RGD1311857"
                     /inference="alignment:Splign:2.1.0"
     exon            1425..1576
                     /gene="Iars2"
                     /gene_synonym="RGD1311857"
                     /inference="alignment:Splign:2.1.0"
     exon            1577..1737
                     /gene="Iars2"
                     /gene_synonym="RGD1311857"
                     /inference="alignment:Splign:2.1.0"
     exon            1738..1840
                     /gene="Iars2"
                     /gene_synonym="RGD1311857"
                     /inference="alignment:Splign:2.1.0"
     exon            1841..1934
                     /gene="Iars2"
                     /gene_synonym="RGD1311857"
                     /inference="alignment:Splign:2.1.0"
     exon            1935..2043
                     /gene="Iars2"
                     /gene_synonym="RGD1311857"
                     /inference="alignment:Splign:2.1.0"
     exon            2044..2146
                     /gene="Iars2"
                     /gene_synonym="RGD1311857"
                     /inference="alignment:Splign:2.1.0"
     exon            2147..2272
                     /gene="Iars2"
                     /gene_synonym="RGD1311857"
                     /inference="alignment:Splign:2.1.0"
     exon            2273..2404
                     /gene="Iars2"
                     /gene_synonym="RGD1311857"
                     /inference="alignment:Splign:2.1.0"
     exon            2405..2511
                     /gene="Iars2"
                     /gene_synonym="RGD1311857"
                     /inference="alignment:Splign:2.1.0"
     exon            2512..2657
                     /gene="Iars2"
                     /gene_synonym="RGD1311857"
                     /inference="alignment:Splign:2.1.0"
     exon            2658..2848
                     /gene="Iars2"
                     /gene_synonym="RGD1311857"
                     /inference="alignment:Splign:2.1.0"
     exon            2849..2993
                     /gene="Iars2"
                     /gene_synonym="RGD1311857"
                     /inference="alignment:Splign:2.1.0"
     exon            2994..3598
                     /gene="Iars2"
                     /gene_synonym="RGD1311857"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gcagttcctc gccgcccaga tcttcaaggg gtccctacat ttatatcccg gacagcagcc
       61 gcgggccttg tgcggagggt ccgcccggtc ctccaccatg ctttgggggc tgcaccctca
      121 cggacccgga acggcggcgc tcaccgcggc tgggagctta tgggggccag cccgttttcc
      181 cagccgcctg gggtgtctgg ggatgacgag gaggctcgtg gtacgctccg tagccggagc
      241 ggacagcccc caaagcagcc cgaaaagcgg cagataccgc gacactgtgc tgctgccgca
      301 gaccaacttc ccgatgaagc tgctgggcca ccagcagtcc gacatggagc tggagatcca
      361 gcagaaatgt ggcttttcag agctttattc ctggcaaaga gaaagaaaag taaagacgga
      421 attttgcctt cacgatggcc ctccctatgc aaatggtgac cctcatgttg ggcatgcctt
      481 aaacaagatt ttgaaagaca ttgtcaatcg attccatatg atgagaggtt ccaaggtaca
      541 ctttgtgcct ggctgggatt gccatggctt gcccattgaa acaaaagtgt tatcagagct
      601 cggggtaaac acccagactc tttcagccat ggagatcagg gagaaagcca ggtcgtttgc
      661 tcaagcagcc attgaaaagc agaaatcggc gtttgcccgc tggggagtga tggcggattg
      721 gagtaactgc tattacacat tcgatccaaa gtatgaagcc aagcagctga gagtatttca
      781 gcaaatgtac gagaagggct tggtttatcg atcttacaaa ccagtgtatt ggtctccctc
      841 atcgaggacg gcgctggctg aagcagagct tgagtacaac cctgagcatg ttagtcggtc
      901 ggtgtatgtg agattccctc tcctgaagcc tccacctacg ctggaatctc tcacaggtgc
      961 ctcatcccct gttagcgctt tagtctggac cacccagcct tggacagttc ctgccaatca
     1021 ggctatatgc tacatgccgg aggccaagta cgctgttgtg aaatgttctg cttctggaaa
     1081 cttctacatc ctggctgaag ataaaatatc acctgttgct tctgccttgg aaacaacatt
     1141 tgatgttact gcaacgtttt caggtgtgga tctggaaggt ggcacttgta gtcacccttt
     1201 aattcccgat aaagtgtctc ctcttttacc tgccactcac gtgactatgg ccaaaggaac
     1261 agggctggtc cacacagcgc cagctcacgg tatggaagat tacagcgtag catcgcagca
     1321 cagcctccct atggactgtt tagtggatga agaaggaatg ttcaccagtg ctgccggccc
     1381 tgaacttcag aacaaggctg tgcttacgga ggggaccgat gtggttataa agatgcttca
     1441 ggctaccaag aatctgttga aagaagagag cattgtgcac agctatccct gtgactggag
     1501 aactaagaaa cccgtgctga ttcgggccag caagcagtgg tttgtaaata tcatggatat
     1561 caaggctaca gctaaggagt cgttaaagac agtgaagttc attcccggag cagcactgaa
     1621 tagcatgatg gacatgctgg acagacggcc gtactggtgc atatcaaggc agagagtctg
     1681 gggtgttcca atccctgtct ttcatcataa gacaaaagat gaatatttga tcaacagcca
     1741 aaccaccgag catattatca aactcgtaga gcagcacggc agtgatgtgt ggtggaccct
     1801 ccccgctgag aagcttctcc cggaggaagt tgtagctcag gctggtggcc ccggtgcttt
     1861 ggagtacgtg ccagggcagg acattttgga catctggttt gacagtggaa cctcgtggtc
     1921 ttgtgttctt caaggcactg agcagagggc agacttgtac ctggagggga aggaccagct
     1981 cgggggctgg ttccagtctt cgttactgac aagtgtggcc acaaggtcaa aagcaccttt
     2041 caggacagtg atggttcatg gatttacact cggagaaaaa ggagaaaaga tgtccaagtc
     2101 cctcgggaac gtcattgacc ctgacactat catcagtgga ggaaaagacc acagcaaaga
     2161 gcctccctac ggagctgaca tcctgcgctg gtggatagcg gaatccaacg tcttcacgga
     2221 ggtgaccatt ggcccctctg tactcagtgc tgccagagat gacatcagca agcttagaaa
     2281 caccctccgc tttctcttgg gaaatttggc cggttttaac ccagagacgg cttctgttcc
     2341 tgtgaagaat atgtacatca tagaccagta tatgctgcac ctaattcagg attttgccac
     2401 aaaggttact gactcataca aacagtatga ctttggaaag gttgtcaggc tgttgaaagc
     2461 cttctacacc agagaactgt ccagctttta cttcagcata gtcaaggata ggctctactg
     2521 tgaaagcgag acggatccca agcggcgctc ctgtcagaca gcattggcgg aaattctgga
     2581 tgtgttggtc cgttcttttg cgcccgttct tcctcacttg gcagaagagg tgttccagca
     2641 catcccctac gttaaagaac ccaagagtgt ttttcgcact ggctggatta gtacaagctc
     2701 tgtttggaag aagccaggat tggaggaggc agtggagagt gcctgtgcta tgcgggattc
     2761 cttccttggc agcatccctg gaaaaaatgc tgccgagtat gacgtcatca tagtgatcga
     2821 gcccgggctg ctgtttgaga tcatggagat gctccaggct gaggagacct ctagcacatc
     2881 tcagctgaat gagttgatga tggcctccca gacaacactg ttggctcagg agccacggga
     2941 aagaagtgca gatgccattg agctcactgg gacattcgtc atcaatctag aaggtggtga
     3001 cattcgtgag gaatcttcat ataaagtcat tgtcgtacca actgccagag agaagtgccc
     3061 gcgatgttgg aagcataccg ctgagactgc agatgctctc tgtcctcggt gtgcagaggt
     3121 cattggtgca aagtagcagt ctgatgtcaa cagctctgaa caagaagctc ccaacagtac
     3181 tgattggctg agggaatgaa ctgtcagaaa gttccctaga aagaaaccca agggagtaga
     3241 ggagtagatg gtaacagatg gggtaagaat cgaagattaa aagagtattt cttgtaacta
     3301 cagaaagaat aatgtgtgtg tgtgtatata tgtgtgtgtg tatatatata tatatatgta
     3361 tatatgcaca cacagatata cacgtataca gagcagacac acacacacac acacacacac
     3421 acacacacac acacacacac acacacacac tcatggactt cttcagagac agacaccgaa
     3481 tatggttact ttcaatgtgg caacagaatg ttttatattt tattaaaact tttttttttt
     3541 tgactcagta tttaagttct gtgatgtctg aaaataaagg cattctggag aactccca
//
