LOCUS       XM_006253706            3902 bp    mRNA    linear   ROD 11-JUN-2023
DEFINITION  PREDICTED: Rattus norvegicus isoleucyl-tRNA synthetase 1 (Iars1),
            transcript variant X2, mRNA.
ACCESSION   XM_006253706
VERSION     XM_006253706.4
DBLINK      BioProject: PRJNA677964
KEYWORDS    RefSeq.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_051352.1) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            [WARNING] On Feb 22, 2024 this sequence was replaced by
            XM_006253706.5.
            
            On Jan 21, 2021 this sequence version replaced XM_006253706.3.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Updated annotation
            Annotation Name             :: GCF_015227675.2-RS_2023_06
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.1
            Annotation Method           :: Best-placed RefSeq; Gnomon;
                                           cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 06/06/2023
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3902
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN/NHsdMcwi"
                     /db_xref="taxon:10116"
                     /chromosome="17"
                     /sex="male"
                     /tissue_type="kidney"
                     /geo_loc_name="USA: Wisconsin, Milwaukee, Medical College
                     of Wisconsin"
                     /collection_date="2019-03-08"
                     /collected_by="Rebecca Schilling"
     gene            1..3902
                     /gene="Iars1"
                     /gene_synonym="Iars; Iarsl"
                     /note="isoleucyl-tRNA synthetase 1; Derived by automated
                     computational analysis using gene prediction method:
                     Gnomon. Supporting evidence includes similarity to: 15
                     ESTs, 5 Proteins, and 99% coverage of the annotated
                     genomic feature by RNAseq alignments, including 144
                     samples with support for all annotated introns"
                     /db_xref="GeneID:306804"
                     /db_xref="RGD:621022"
     CDS             114..3902
                     /gene="Iars1"
                     /gene_synonym="Iars; Iarsl"
                     /codon_start=1
                     /product="isoleucine--tRNA ligase, cytoplasmic isoform X1"
                     /protein_id="XP_006253768.1"
                     /db_xref="GeneID:306804"
                     /db_xref="RGD:621022"
                     /translation="MVQQVPENISFPAEEEKILQFWSKYNCFQECLKQSKLRPKFTFY
                     DGPPFATGLPHYGHILAGTIKDIVTRYAHQSGFHVDRRFGWDCHGLPVEYEIDKTLGI
                     KGPEDVAKMGIAEYNKQCRAIVMRYSAEWKSTVTRLGRWIDFDNDYKTLYPQFMESVW
                     WVFKQLYDKGLVYRGVKVMPFSTACGTPLSNFESNQNYKDVQDPSVFVTFPLEEDENT
                     SLVAWTTTPWTLPSNLALCVNPEIQYVKIKDVSRGKLFILMEARLSALYKQESDYEIL
                     ERFPGASLKGKKYKPLFDYFIKCKENGAFTVLVDHYVKDEEGTGVVHQAPYFGADDHR
                     VCMDFNIIQKDSAPVCPVDASGCFTEEVTHFVGQYVKDADKNIIRMLKEQGRLLVAST
                     FTHSYPFCWRSDTPLIYKSVPSWFVRVEHMVDQLLKNNDLCYWVPEFVREKRFGNWLK
                     EARDWAISRNRYWGTPIPLWVSEDFEEVVCIGSVAELEELSGTKISDLHRESIDHLTI
                     PSRCGKGPLRRVSEVFDCWFESGSMPYAQVHYPFESKREFEDAFPADFIAEGIDQTRG
                     WFYTLLVLATALFGQPPFKNVIVNGLILASDGQKMSKRKKNYPDPVSIINKYGADALR
                     LYLINSPVVRAENLRFKEEGVRDVLKDVLLPWYNAYRFFIQNVFRLHKEEEVEFLYNE
                     HTVRESPNITDRWILSFMQSLIAFFETEMAAYRLYTVVPRLVKFVDILTNWYVRMNRR
                     RLKGENGMEDCVMALETLFSVLLSLCRLMAPYTPFLTELMYQNLKLLIDPASIRDKDT
                     LSIHYLMLPHVREELIDKKTENAVSRMQSVIELGRVIRDRKTIPIKYPLKEIVVIHQD
                     PEALEDIKSLEKYIIEELNVRKVTLSTDKNKYGIRLRAEPDHMVLGKRLKGAFKAVMV
                     AVKQLSNEELEQFQKSGSIVVEGHELHEEDIRLMYTFDQATGGTAQFEAHSDAQALVL
                     LDVTPDQSMVDEGMAREVINRIQKLRKKCNLVPTDEITVYYNAKSEGRYLSNVIESHT
                     DFIYATIKAPLKPYPVPTSDNILIQEQTQLKGYELEITITRGSCVPGPACAYVNLNIC
                     ANGTEQGGVLLLENPKGDNQLNLEKLKSVITSIFGVKSTGLSVFNGGTELQNQTDLLS
                     LSGRTLCVTAGASLAPASSPSTLLCQYINLQLVNAEPQECLTGTVGTLLLENPLGQNG
                     LTHQGLVYEAAKVFGLRSRRLKLFLNETQTQEITEDIPMKTLNMKTVYVSVLPTTADG
                     "
ORIGIN      
        1 gacggcgcgg aggggttgag cctggcactg tcagtattgc atcacctcca gacgcggaac
       61 gtggtcgcag tctgcaacct gagcttgctc acctcagggt tcccagcaac agaatggttc
      121 aacaagttcc ggaaaacatc agctttcctg ctgaagaaga aaaaatctta cagttctggt
      181 ccaaatacaa ctgtttccag gaatgcctaa agcagtcaaa gctcaggcca aagtttacct
      241 tctatgatgg gcctcccttc gctactggac tgcctcacta cggacacata cttgcgggga
      301 caattaaaga tatagttaca agatacgctc accagagcgg gtttcacgtc gacagaagat
      361 tcgggtggga ttgtcatggc ttacctgtgg agtacgagat tgacaagacc ctggggatca
      421 aagggccaga ggacgtggcc aagatgggga tcgcagagta caacaagcag tgccgagcta
      481 ttgtgatgag gtactccgcc gagtggaagt ctactgttac caggcttggc cgatggatcg
      541 actttgacaa tgactacaaa accctgtacc cacaattcat ggaatcagtc tggtgggtct
      601 tcaagcagct gtatgacaaa ggcctcgtgt acagaggcgt gaaagtcatg cccttctcca
      661 ccgcgtgtgg aactccactc tccaacttcg agtcgaacca gaattacaag gacgttcagg
      721 acccttcagt atttgtaacg ttccctttgg aggaagatga aaacacatcc ttagttgctt
      781 ggacaaccac tccgtggacc ctacccagta accttgcgct atgtgtcaat ccagaaatac
      841 agtatgtgaa gatcaaagac gtttccaggg ggaagttgtt cattttgatg gaagccagat
      901 tgtcggccct ctataaacaa gagagcgact atgagatcct tgaaaggttt cctggtgcct
      961 ctctcaaagg caagaaatac aagccgttgt ttgactactt tattaagtgt aaggagaacg
     1021 gtgccttcac cgtgcttgtt gaccactatg tgaaggatga agaaggcaca ggtgtcgttc
     1081 accaggctcc ttactttggc gctgacgatc atcgagtctg tatggacttc aacatcattc
     1141 agaaagactc agcccctgtt tgtcctgtgg atgcttcagg ctgcttcaca gaggaagtga
     1201 cacattttgt cgggcagtat gtgaaggatg ctgacaaaaa tatcatcagg atgttgaagg
     1261 agcagggccg actgctcgtg gcaagcacct tcactcacag ctaccctttc tgctggaggt
     1321 cggacactcc cctcatttac aagtccgtgc ccagctggtt cgtgcgggtg gagcacatgg
     1381 tggaccagct actgaagaac aatgatctgt gttactgggt cccagagttt gtacgagaaa
     1441 aacggtttgg aaattggctg aaggaggcac gtgactgggc aatttcccga aacagatact
     1501 ggggcacccc catcccactg tgggtcagtg aagactttga ggaggtagtg tgcatcgggt
     1561 cagtggcaga acttgaagaa ttgtcaggaa caaaaatctc agatctccac agagagagca
     1621 tcgaccacct gaccatacca tcccgctgcg ggaaaggacc gttgcggcgt gtctctgagg
     1681 tgtttgactg ttggttcgag agtggaagta tgccttacgc ccaggttcat tacccctttg
     1741 agagcaagcg agagttcgag gatgcctttc ccgcagactt cattgctgaa ggcatcgatc
     1801 aaaccagagg atggttttac accctgctag tgctagccac agccctcttt ggacaaccac
     1861 ctttcaagaa cgtgattgtg aacggcctca tcttggcaag tgatggccaa aaaatgagca
     1921 aacggaaaaa gaactaccca gatccggttt ccatcatcaa taagtacggt gccgacgccc
     1981 tcaggttata tctgatcaat tcccctgtgg tgagagcaga aaacctgcgc ttcaaggagg
     2041 aaggcgtacg ggatgtcctc aaagacgtgc tgctcccatg gtacaacgca taccgcttct
     2101 tcatccagaa cgtctttagg ctgcacaagg aagaagaagt agagtttctg tacaacgagc
     2161 acacggtccg agaaagcccc aacatcaccg accggtggat tctgtccttc atgcagtcgc
     2221 tcattgcgtt ctttgagacg gaaatggcag cttacaggct gtacactgtg gtgcctcgcc
     2281 tggtcaagtt cgtagatatt ctgaccaact ggtacgtccg aatgaacagg agaagattaa
     2341 agggggagaa tggcatggaa gactgtgtta tggctctgga gactctgttc agtgtcctgc
     2401 tttctctgtg cagactgatg gccccttaca caccctttct tactgaactg atgtaccaga
     2461 acctgaagct gctgattgac cctgcatcta tccgggacaa ggacaccctc agcatccact
     2521 acctcatgct gcctcatgtt cgagaggagc tgattgacaa gaaaactgag aacgccgtgt
     2581 ctagaatgca gtccgtgatt gagcttggga gagtgattcg agaccggaaa actattccaa
     2641 taaagtatcc cttgaaggaa attgtcgtca tccaccaaga cccagaagct cttgaggata
     2701 tcaagtcctt ggagaagtat atcattgagg aactaaatgt tcgaaaagtt acactgtcta
     2761 ctgataaaaa caagtatggc attcgactga gggctgagcc agatcacatg gtccttggga
     2821 aacgtctgaa gggagccttt aaagcagtga tggtggccgt aaagcagctg agcaatgagg
     2881 agctggagca gtttcagaag agtgggagca tcgtcgtgga aggccatgaa ttacacgaag
     2941 aagatattcg tctcatgtac acctttgacc aggccacagg aggcactgca cagtttgaag
     3001 cacactctga tgctcaggct ttagttctct tagatgtcac acctgaccag tcaatggtgg
     3061 acgaaggcat ggctcgggaa gtcatcaatc gcatccagaa gcttcgcaag aagtgcaacc
     3121 tggttccgac ggatgaaata acagtttact ataatgccaa atctgaagga agatacttga
     3181 gtaatgtcat cgaaagccac actgacttca tatatgccac tatcaaagct cccttgaagc
     3241 cctatccagt tcctacatca gataatatcc ttattcaaga gcaaacacag ttaaaggggt
     3301 acgagctgga aatcacgatc accagaggat cctgtgtgcc tgggcccgct tgtgcttatg
     3361 tgaaccttaa catttgtgca aatggcactg agcaaggtgg agtgttactc ctggagaatc
     3421 cgaagggtga taatcagttg aaccttgaga agctgaagag cgtcattacc agcatattcg
     3481 gtgttaaaag cacagggctg tcagtcttca acggtggaac agaattacag aaccagactg
     3541 acttgctgag cctcagtgga aggacgctgt gtgtgactgc aggagcatcc ctggctccag
     3601 ccagcagccc cagcaccctt ctctgtcagt acatcaacct gcagctcgtg aacgcagagc
     3661 cacaagagtg tttaacagga actgtgggca ccctcctgct tgaaaaccca cttggacaga
     3721 acgggctcac ccaccaaggg cttgtgtacg aagcagccaa ggtgtttgga ctgcgcagcc
     3781 ggaggctaaa gctgttcctg aatgagactc agacacaaga aattacagaa gatatcccaa
     3841 tgaagacttt gaatatgaag actgtatatg tttctgtctt gccaacgact gcagatggct
     3901 aa
//
