LOCUS       NM_001100572            4494 bp    mRNA    linear   ROD 31-MAR-2024
DEFINITION  Rattus norvegicus isoleucyl-tRNA synthetase 1 (Iars1), mRNA.
ACCESSION   NM_001100572 XM_001055989 XM_225196
VERSION     NM_001100572.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 4494)
  AUTHORS   Forster,F., Paster,W., Supper,V., Schatzlmaier,P., Sunzenauer,S.,
            Ostler,N., Saliba,A., Eckerstorfer,P., Britzen-Laurent,N.,
            Schutz,G., Schmid,J.A., Zlabinger,G.J., Naschberger,E., Sturzl,M.
            and Stockinger,H.
  TITLE     Guanylate binding protein 1-mediated interaction of T cell antigen
            receptor signaling with the cytoskeleton
  JOURNAL   J Immunol 192 (2), 771-781 (2014)
   PUBMED   24337748
REFERENCE   2  (bases 1 to 4494)
  AUTHORS   Han,J.M., Jeong,S.J., Park,M.C., Kim,G., Kwon,N.H., Kim,H.K.,
            Ha,S.H., Ryu,S.H. and Kim,S.
  TITLE     Leucyl-tRNA synthetase is an intracellular leucine sensor for the
            mTORC1-signaling pathway
  JOURNAL   Cell 149 (2), 410-424 (2012)
   PUBMED   22424946
REFERENCE   3  (bases 1 to 4494)
  AUTHORS   Buschow,S.I., van Balkom,B.W., Aalberts,M., Heck,A.J., Wauben,M.
            and Stoorvogel,W.
  TITLE     MHC class II-associated proteins in B-cell exosomes and potential
            functional implications for exosome biogenesis
  JOURNAL   Immunol Cell Biol 88 (8), 851-856 (2010)
   PUBMED   20458337
REFERENCE   4  (bases 1 to 4494)
  AUTHORS   Ghosh,D., Lippert,D., Krokhin,O., Cortens,J.P. and Wilkins,J.A.
  TITLE     Defining the membrane proteome of NK cells
  JOURNAL   J Mass Spectrom 45 (1), 1-25 (2010)
   PUBMED   19946888
REFERENCE   5  (bases 1 to 4494)
  AUTHORS   Kaminska,M., Havrylenko,S., Decottignies,P., Le Marechal,P.,
            Negrutskii,B. and Mirande,M.
  TITLE     Dynamic Organization of Aminoacyl-tRNA Synthetase Complexes in the
            Cytoplasm of Human Cells
  JOURNAL   J Biol Chem 284 (20), 13746-13754 (2009)
   PUBMED   19289464
REFERENCE   6  (bases 1 to 4494)
  AUTHORS   Kaminska,M., Havrylenko,S., Decottignies,P., Gillet,S., Le
            Marechal,P., Negrutskii,B. and Mirande,M.
  TITLE     Dissection of the structural organization of the aminoacyl-tRNA
            synthetase complex
  JOURNAL   J Biol Chem 284 (10), 6053-6060 (2009)
   PUBMED   19131329
REFERENCE   7  (bases 1 to 4494)
  AUTHORS   Foster,L.J., Zeemann,P.A., Li,C., Mann,M., Jensen,O.N. and
            Kassem,M.
  TITLE     Differential expression profiling of membrane proteins by
            quantitative proteomics in a human mesenchymal stem cell line
            undergoing osteoblast differentiation
  JOURNAL   Stem Cells 23 (9), 1367-1377 (2005)
   PUBMED   16210410
REFERENCE   8  (bases 1 to 4494)
  AUTHORS   Kim,J.Y., Kang,Y.S., Lee,J.W., Kim,H.J., Ahn,Y.H., Park,H., Ko,Y.G.
            and Kim,S.
  TITLE     p38 is essential for the assembly and stability of macromolecular
            tRNA synthetase complex: implications for its physiological
            significance
  JOURNAL   Proc Natl Acad Sci U S A 99 (12), 7912-7916 (2002)
   PUBMED   12060739
REFERENCE   9  (bases 1 to 4494)
  AUTHORS   Adams,L.D., Lemire,J.M. and Schwartz,S.M.
  TITLE     A systematic analysis of 40 random genes in cultured vascular
            smooth muscle subtypes reveals a heterogeneity of gene expression
            and identifies the tight junction gene zonula occludens 2 as a
            marker of epithelioid 'pup' smooth muscle cells and a participant
            in carotid neointimal formation
  JOURNAL   Arterioscler Thromb Vasc Biol 19 (11), 2600-2608 (1999)
   PUBMED   10559001
REFERENCE   10 (bases 1 to 4494)
  AUTHORS   Shiba,K., Suzuki,N., Shigesada,K., Namba,Y., Schimmel,P. and
            Noda,T.
  TITLE     Human cytoplasmic isoleucyl-tRNA synthetase: selective divergence
            of the anticodon-binding domain and acquisition of a new structural
            unit
  JOURNAL   Proc Natl Acad Sci U S A 91 (16), 7435-7439 (1994)
   PUBMED   8052601
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000017.1.
            
            On or before Oct 3, 2008 this sequence version replaced
            XM_001055989.1, XM_225196.4.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR26360190.1628187.1,
                                           SRR26643294.55059.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA5760383,
                                           SAMEA5760386 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-203               JAXUCZ010000017.1  15193450-15193652   c
            204-329             JAXUCZ010000017.1  15188732-15188857   c
            330-486             JAXUCZ010000017.1  15188040-15188196   c
            487-606             JAXUCZ010000017.1  15187700-15187819   c
            607-689             JAXUCZ010000017.1  15185840-15185922   c
            690-807             JAXUCZ010000017.1  15184305-15184422   c
            808-955             JAXUCZ010000017.1  15182956-15183103   c
            956-1043            JAXUCZ010000017.1  15181055-15181142   c
            1044-1104           JAXUCZ010000017.1  15180398-15180458   c
            1105-1200           JAXUCZ010000017.1  15179643-15179738   c
            1201-1323           JAXUCZ010000017.1  15178202-15178324   c
            1324-1415           JAXUCZ010000017.1  15177607-15177698   c
            1416-1514           JAXUCZ010000017.1  15176129-15176227   c
            1515-1641           JAXUCZ010000017.1  15175082-15175208   c
            1642-1715           JAXUCZ010000017.1  15173005-15173078   c
            1716-1910           JAXUCZ010000017.1  15171723-15171917   c
            1911-1997           JAXUCZ010000017.1  15169909-15169995   c
            1998-2081           JAXUCZ010000017.1  15167585-15167668   c
            2082-2226           JAXUCZ010000017.1  15166619-15166763   c
            2227-2347           JAXUCZ010000017.1  15164924-15165044   c
            2348-2439           JAXUCZ010000017.1  15163544-15163635   c
            2440-2517           JAXUCZ010000017.1  15161974-15162051   c
            2518-2639           JAXUCZ010000017.1  15161004-15161125   c
            2640-2742           JAXUCZ010000017.1  15160202-15160304   c
            2743-2826           JAXUCZ010000017.1  15159913-15159996   c
            2827-3001           JAXUCZ010000017.1  15157260-15157434   c
            3002-3114           JAXUCZ010000017.1  15156749-15156861   c
            3115-3210           JAXUCZ010000017.1  15156043-15156138   c
            3211-3387           JAXUCZ010000017.1  15155023-15155199   c
            3388-3493           JAXUCZ010000017.1  15153700-15153805   c
            3494-3619           JAXUCZ010000017.1  15151033-15151158   c
            3620-3763           JAXUCZ010000017.1  15150084-15150227   c
            3764-3916           JAXUCZ010000017.1  15149219-15149371   c
            3917-4494           JAXUCZ010000017.1  15147357-15147934   c
FEATURES             Location/Qualifiers
     source          1..4494
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="17"
                     /map="17p14"
     gene            1..4494
                     /gene="Iars1"
                     /gene_synonym="Iars; Iarsl"
                     /note="isoleucyl-tRNA synthetase 1"
                     /db_xref="GeneID:306804"
                     /db_xref="RGD:621022"
     exon            1..203
                     /gene="Iars1"
                     /gene_synonym="Iars; Iarsl"
                     /inference="alignment:Splign:2.1.0"
     exon            204..329
                     /gene="Iars1"
                     /gene_synonym="Iars; Iarsl"
                     /inference="alignment:Splign:2.1.0"
     CDS             211..3999
                     /gene="Iars1"
                     /gene_synonym="Iars; Iarsl"
                     /EC_number="6.1.1.5"
                     /note="isoleucine-tRNA synthetase; isoleucine--tRNA
                     ligase, cytoplasmic-like; isoleucyl-tRNA synthetase,
                     cytoplasmic; isoleucyl tRNA synthetase"
                     /codon_start=1
                     /product="isoleucine--tRNA ligase, cytoplasmic"
                     /protein_id="NP_001094042.1"
                     /db_xref="GeneID:306804"
                     /db_xref="RGD:621022"
                     /translation="MVQQVPENISFPAEEEKILQFWSKYNCFQECLKQSKLRPKFTFY
                     DGPPFATGLPHYGHILAGTIKDIVTRYAHQSGFHVDRRFGWDCHGLPVEYEIDKTLGI
                     KGPEDVAKMGIAEYNKQCRAIVMRYSAEWKSTVTRLGRWIDFDNDYKTLYPQFMESVW
                     WVFKQLYDKGLVYRGVKVMPFSTACGTPLSNFESNQNYKDVQDPSVFVTFPLEEDENT
                     SLVAWTTTPWTLPSNLALCVNPEIQYVKIKDVSRGKLFILMEARLSALYKQESDYEIL
                     ERFPGASLKGKKYKPLFDYFIKCKENGAFTVLVDHYVKDEEGTGVVHQAPYFGADDHR
                     VCMDFNIIQKDSAPVCPVDASGCFTEEVTHFVGQYVKDADKNIIRMLKEQGRLLVAST
                     FTHSYPFCWRSDTPLIYKSVPSWFVRVEHMVDQLLKNNDLCYWVPEFVREKRFGNWLK
                     EARDWAISRNRYWGTPIPLWVSEDFEEVVCIGSVAELEELSGTKISDLHRESIDHLTI
                     PSRCGKGPLRRVSEVFDCWFESGSMPYAQVHYPFESKREFEDAFPADFIAEGIDQTRG
                     WFYTLLVLATALFGQPPFKNVIVNGLILASDGQKMSKRKKNYPDPVSIINKYGADALR
                     LYLINSPVVRAENLRFKEEGVRDVLKDVLLPWYNAYRFFIQNVFRLHKEEEVEFLYNE
                     HTVRESPNITDRWILSFMQSLIAFFETEMAAYRLYTVVPRLVKFVDILTNWYVRMNRR
                     RLKGENGMEDCVMALETLFSVLLSLCRLMAPYTPFLTELMYQNLKLLIDPASIRDKDT
                     LSIHYLMLPHVREELIDKKTENAVSRMQSVIELGRVIRDRKTIPIKYPLKEIVVIHQD
                     PEALEDIKSLEKYIIEELNVRKVTLSTDKNKYGIRLRAEPDHMVLGKRLKGAFKAVMV
                     AVKQLSNEELEQFQKSGSIVVEGHELHEEDIRLMYTFDQATGGTAQFEAHSDAQALVL
                     LDVTPDQSMVDEGMAREVINRIQKLRKKCNLVPTDEITVYYNAKSEGRYLSNVIESHT
                     DFIYATIKAPLKPYPVPTSDNILIQEQTQLKGYELEITITRGSCVPGPACAYVNLNIC
                     ANGTEQGGVLLLENPKGDNQLNLEKLKSVITSIFGVKSTGLSVFNGGTELQNQTDLLS
                     LSGRTLCVTAGASLAPASSPSTLLCQYINLQLVNAEPQECLTGTVGTLLLENPLGQNG
                     LTHQGLVYEAAKVFGLRSRRLKLFLNETQTQEITEDIPMKTLNMKTVYVSVLPTTADG
                     "
     exon            330..486
                     /gene="Iars1"
                     /gene_synonym="Iars; Iarsl"
                     /inference="alignment:Splign:2.1.0"
     exon            487..606
                     /gene="Iars1"
                     /gene_synonym="Iars; Iarsl"
                     /inference="alignment:Splign:2.1.0"
     exon            607..689
                     /gene="Iars1"
                     /gene_synonym="Iars; Iarsl"
                     /inference="alignment:Splign:2.1.0"
     exon            690..807
                     /gene="Iars1"
                     /gene_synonym="Iars; Iarsl"
                     /inference="alignment:Splign:2.1.0"
     exon            808..955
                     /gene="Iars1"
                     /gene_synonym="Iars; Iarsl"
                     /inference="alignment:Splign:2.1.0"
     exon            956..1043
                     /gene="Iars1"
                     /gene_synonym="Iars; Iarsl"
                     /inference="alignment:Splign:2.1.0"
     exon            1044..1104
                     /gene="Iars1"
                     /gene_synonym="Iars; Iarsl"
                     /inference="alignment:Splign:2.1.0"
     exon            1105..1200
                     /gene="Iars1"
                     /gene_synonym="Iars; Iarsl"
                     /inference="alignment:Splign:2.1.0"
     exon            1201..1323
                     /gene="Iars1"
                     /gene_synonym="Iars; Iarsl"
                     /inference="alignment:Splign:2.1.0"
     exon            1324..1415
                     /gene="Iars1"
                     /gene_synonym="Iars; Iarsl"
                     /inference="alignment:Splign:2.1.0"
     exon            1416..1514
                     /gene="Iars1"
                     /gene_synonym="Iars; Iarsl"
                     /inference="alignment:Splign:2.1.0"
     exon            1515..1641
                     /gene="Iars1"
                     /gene_synonym="Iars; Iarsl"
                     /inference="alignment:Splign:2.1.0"
     exon            1642..1715
                     /gene="Iars1"
                     /gene_synonym="Iars; Iarsl"
                     /inference="alignment:Splign:2.1.0"
     exon            1716..1910
                     /gene="Iars1"
                     /gene_synonym="Iars; Iarsl"
                     /inference="alignment:Splign:2.1.0"
     exon            1911..1997
                     /gene="Iars1"
                     /gene_synonym="Iars; Iarsl"
                     /inference="alignment:Splign:2.1.0"
     exon            1998..2081
                     /gene="Iars1"
                     /gene_synonym="Iars; Iarsl"
                     /inference="alignment:Splign:2.1.0"
     exon            2082..2226
                     /gene="Iars1"
                     /gene_synonym="Iars; Iarsl"
                     /inference="alignment:Splign:2.1.0"
     exon            2227..2347
                     /gene="Iars1"
                     /gene_synonym="Iars; Iarsl"
                     /inference="alignment:Splign:2.1.0"
     exon            2348..2439
                     /gene="Iars1"
                     /gene_synonym="Iars; Iarsl"
                     /inference="alignment:Splign:2.1.0"
     exon            2440..2517
                     /gene="Iars1"
                     /gene_synonym="Iars; Iarsl"
                     /inference="alignment:Splign:2.1.0"
     exon            2518..2639
                     /gene="Iars1"
                     /gene_synonym="Iars; Iarsl"
                     /inference="alignment:Splign:2.1.0"
     exon            2640..2742
                     /gene="Iars1"
                     /gene_synonym="Iars; Iarsl"
                     /inference="alignment:Splign:2.1.0"
     exon            2743..2826
                     /gene="Iars1"
                     /gene_synonym="Iars; Iarsl"
                     /inference="alignment:Splign:2.1.0"
     exon            2827..3001
                     /gene="Iars1"
                     /gene_synonym="Iars; Iarsl"
                     /inference="alignment:Splign:2.1.0"
     exon            3002..3114
                     /gene="Iars1"
                     /gene_synonym="Iars; Iarsl"
                     /inference="alignment:Splign:2.1.0"
     exon            3115..3210
                     /gene="Iars1"
                     /gene_synonym="Iars; Iarsl"
                     /inference="alignment:Splign:2.1.0"
     exon            3211..3387
                     /gene="Iars1"
                     /gene_synonym="Iars; Iarsl"
                     /inference="alignment:Splign:2.1.0"
     exon            3388..3493
                     /gene="Iars1"
                     /gene_synonym="Iars; Iarsl"
                     /inference="alignment:Splign:2.1.0"
     exon            3494..3619
                     /gene="Iars1"
                     /gene_synonym="Iars; Iarsl"
                     /inference="alignment:Splign:2.1.0"
     exon            3620..3763
                     /gene="Iars1"
                     /gene_synonym="Iars; Iarsl"
                     /inference="alignment:Splign:2.1.0"
     exon            3764..3916
                     /gene="Iars1"
                     /gene_synonym="Iars; Iarsl"
                     /inference="alignment:Splign:2.1.0"
     exon            3917..4494
                     /gene="Iars1"
                     /gene_synonym="Iars; Iarsl"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gtggtcgcag tctgcaacct gagcttgctc acctcagggt tcccaggtga gccgcaccct
       61 ccgcccttcc gggcctcaga gcgaggcctg ccggtgtaac tatctgtgga tagcaccagg
      121 cggtgaaaaa ggagcacgct gattccttcc gtgagtgagc tgcgggcaca gagctatttc
      181 cacagagcct tcgtggaggg agacaacaga atggttcaac aagttccgga aaacatcagc
      241 tttcctgctg aagaagaaaa aatcttacag ttctggtcca aatacaactg tttccaggaa
      301 tgcctaaagc agtcaaagct caggccaaag tttaccttct atgatgggcc tcccttcgct
      361 actggactgc ctcactacgg acacatactt gcggggacaa ttaaagatat agttacaaga
      421 tacgctcacc agagcgggtt tcacgtcgac agaagattcg ggtgggattg tcatggctta
      481 cctgtggagt acgagattga caagaccctg gggatcaaag ggccagagga cgtggccaag
      541 atggggatcg cagagtacaa caagcagtgc cgagctattg tgatgaggta ctccgccgag
      601 tggaagtcta ctgttaccag gcttggccga tggatcgact ttgacaatga ctacaaaacc
      661 ctgtacccac aattcatgga atcagtctgg tgggtcttca agcagctgta tgacaaaggc
      721 ctcgtgtaca gaggcgtgaa agtcatgccc ttctccaccg cgtgtggaac tccactctcc
      781 aacttcgagt cgaaccagaa ttacaaggac gttcaggacc cttcagtatt tgtaacgttc
      841 cctttggagg aagatgaaaa cacatcctta gttgcttgga caaccactcc gtggacccta
      901 cccagtaacc ttgcgctatg tgtcaatcca gaaatacagt atgtgaagat caaagacgtt
      961 tccaggggga agttgttcat tttgatggaa gccagattgt cggccctcta taaacaagag
     1021 agcgactatg agatccttga aaggtttcct ggtgcctctc tcaaaggcaa gaaatacaag
     1081 ccgttgtttg actactttat taagtgtaag gagaacggtg ccttcaccgt gcttgttgac
     1141 cactatgtga aggatgaaga aggcacaggt gtcgttcacc aggctcctta ctttggcgct
     1201 gacgatcatc gagtctgtat ggacttcaac atcattcaga aagactcagc ccctgtttgt
     1261 cctgtggatg cttcaggctg cttcacagag gaagtgacac attttgtcgg gcagtatgtg
     1321 aaggatgctg acaaaaatat catcaggatg ttgaaggagc agggccgact gctcgtggca
     1381 agcaccttca ctcacagcta ccctttctgc tggaggtcgg acactcccct catttacaag
     1441 tccgtgccca gctggttcgt gcgggtggag cacatggtgg accagctact gaagaacaat
     1501 gatctgtgtt actgggtccc agagtttgta cgagaaaaac ggtttggaaa ttggctgaag
     1561 gaggcacgtg actgggcaat ttcccgaaac agatactggg gcacccccat cccactgtgg
     1621 gtcagtgaag actttgagga ggtagtgtgc atcgggtcag tggcagaact tgaagaattg
     1681 tcaggaacaa aaatctcaga tctccacaga gagagcatcg accacctgac cataccatcc
     1741 cgctgcggga aaggaccgtt gcggcgtgtc tctgaggtgt ttgactgttg gttcgagagt
     1801 ggaagtatgc cttacgccca ggttcattac ccctttgaga gcaagcgaga gttcgaggat
     1861 gcctttcccg cagacttcat tgctgaaggc atcgatcaaa ccagaggatg gttttacacc
     1921 ctgctagtgc tagccacagc cctctttgga caaccacctt tcaagaacgt gattgtgaac
     1981 ggcctcatct tggcaagtga tggccaaaaa atgagcaaac ggaaaaagaa ctacccagat
     2041 ccggtttcca tcatcaataa gtacggtgcc gacgccctca ggttatatct gatcaattcc
     2101 cctgtggtga gagcagaaaa cctgcgcttc aaggaggaag gcgtacggga tgtcctcaaa
     2161 gacgtgctgc tcccatggta caacgcatac cgcttcttca tccagaacgt ctttaggctg
     2221 cacaaggaag aagaagtaga gtttctgtac aacgagcaca cggtccgaga aagccccaac
     2281 atcaccgacc ggtggattct gtccttcatg cagtcgctca ttgcgttctt tgagacggaa
     2341 atggcagctt acaggctgta cactgtggtg cctcgcctgg tcaagttcgt agatattctg
     2401 accaactggt acgtccgaat gaacaggaga agattaaagg gggagaatgg catggaagac
     2461 tgtgttatgg ctctggagac tctgttcagt gtcctgcttt ctctgtgcag actgatggcc
     2521 ccttacacac cctttcttac tgaactgatg taccagaacc tgaagctgct gattgaccct
     2581 gcatctatcc gggacaagga caccctcagc atccactacc tcatgctgcc tcatgttcga
     2641 gaggagctga ttgacaagaa aactgagaac gccgtgtcta gaatgcagtc cgtgattgag
     2701 cttgggagag tgattcgaga ccggaaaact attccaataa agtatccctt gaaggaaatt
     2761 gtcgtcatcc accaagaccc agaagctctt gaggatatca agtccttgga gaagtatatc
     2821 attgaggaac taaatgttcg aaaagttaca ctgtctactg ataaaaacaa gtatggcatt
     2881 cgactgaggg ctgagccaga tcacatggtc cttgggaaac gtctgaaggg agcctttaaa
     2941 gcagtgatgg tggccgtaaa gcagctgagc aatgaggagc tggagcagtt tcagaagagt
     3001 gggagcatcg tcgtggaagg ccatgaatta cacgaagaag atattcgtct catgtacacc
     3061 tttgaccagg ccacaggagg cactgcacag tttgaagcac actctgatgc tcaggcttta
     3121 gttctcttag atgtcacacc tgaccagtca atggtggacg aaggcatggc tcgggaagtc
     3181 atcaatcgca tccagaagct tcgcaagaag tgcaacctgg ttccgacgga tgaaataaca
     3241 gtttactata atgccaaatc tgaaggaaga tacttgagta atgtcatcga aagccacact
     3301 gacttcatat atgccactat caaagctccc ttgaagccct atccagttcc tacatcagat
     3361 aatatcctta ttcaagagca aacacagtta aaggggtacg agctggaaat cacgatcacc
     3421 agaggatcct gtgtgcctgg gcccgcttgt gcttatgtga accttaacat ttgtgcaaat
     3481 ggcactgagc aaggtggagt gttactcctg gagaatccga agggtgataa tcagttgaac
     3541 cttgagaagc tgaagagcgt cattaccagc atattcggtg ttaaaagcac agggctgtca
     3601 gtcttcaacg gtggaacaga attacagaac cagactgact tgctgagcct cagtggaagg
     3661 acgctgtgtg tgactgcagg agcatccctg gctccagcca gcagccccag cacccttctc
     3721 tgtcagtaca tcaacctgca gctcgtgaac gcagagccac aagagtgttt aacaggaact
     3781 gtgggcaccc tcctgcttga aaacccactt ggacagaacg ggctcaccca ccaagggctt
     3841 gtgtacgaag cagccaaggt gtttggactg cgcagccgga ggctaaagct gttcctgaat
     3901 gagactcaga cacaagaaat tacagaagat atcccaatga agactttgaa tatgaagact
     3961 gtatatgttt ctgtcttgcc aacgactgca gatggctaac agttagttat cagtatgccc
     4021 tcagtcagcc tgccttcctg aatctgccca tctccttaca cacacacaca cacacacaca
     4081 cacacacaca cacacacaca cacacacaga ggctcagtga cggtgtctcc ctgggactcg
     4141 tgtctaatat gctgctttga accttcaatt caaatgggga ttgactgact tgagacctca
     4201 cataatgttc acatttaacc ttaaacacct atgcagtcct gtgggggagg gtcctaacgt
     4261 tacctcagca ccacaaagct tatttctata ctcgagttct tcagtacaga aagcggaagt
     4321 ggtttaaatg gcatagtaca tatacataca tatatatata gccttgtaaa actgatttgt
     4381 gaccttgtga agaaatggat atgttatata tttctgctac ctggattttc ctgggtaatt
     4441 tgatgaaata ttttaagttt gattaaatca aagtaaacac tctctcagat actg
//
