LOCUS       NM_012586               2345 bp    mRNA    linear   ROD 06-NOV-2024
DEFINITION  Rattus norvegicus islet amyloid polypeptide (Iapp), mRNA.
ACCESSION   NM_012586 XM_006237614
VERSION     NM_012586.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 2345)
  AUTHORS   Sanders,H.M., Chalyavi,F., Fields,C.R., Kostelic,M.M., Li,M.H.,
            Raleigh,D.P., Zanni,M.T. and Marty,M.T.
  TITLE     Interspecies Variation Affects Islet Amyloid Polypeptide Membrane
            Binding
  JOURNAL   J Am Soc Mass Spectrom 34 (6), 986-990 (2023)
   PUBMED   37126782
  REMARK    GeneRIF: Interspecies Variation Affects Islet Amyloid Polypeptide
            Membrane Binding.
REFERENCE   2  (bases 1 to 2345)
  AUTHORS   Cao,J., Belousoff,M.J., Liang,Y.L., Johnson,R.M., Josephs,T.M.,
            Fletcher,M.M., Christopoulos,A., Hay,D.L., Danev,R., Wootten,D. and
            Sexton,P.M.
  TITLE     A structural basis for amylin receptor phenotype
  JOURNAL   Science 375 (6587), eabm9609 (2022)
   PUBMED   35324283
REFERENCE   3  (bases 1 to 2345)
  AUTHORS   Babych,M., Nguyen,P.T., Cote-Cyr,M., Kihal,N., Quittot,N.,
            Golizeh,M., Sleno,L. and Bourgault,S.
  TITLE     Site-Specific Alkylation of the Islet Amyloid Polypeptide
            Accelerates Self-Assembly and Potentiates Perturbation of Lipid
            Membranes
  JOURNAL   Biochemistry 60 (29), 2285-2299 (2021)
   PUBMED   34264642
  REMARK    GeneRIF: Site-Specific Alkylation of the Islet Amyloid Polypeptide
            Accelerates Self-Assembly and Potentiates Perturbation of Lipid
            Membranes.
REFERENCE   4  (bases 1 to 2345)
  AUTHORS   Liu,X., Yang,R., Bai,W., Xu,X., Bi,F., Hao,Y., Yang,Q. and Li,H.
  TITLE     Involvement of amylin B-H2S-connexin 43 signaling pathway in
            vascular dysfunction and enhanced ischemia-reperfusion-induced
            myocardial injury in diabetic rats
  JOURNAL   Biosci Rep 40 (6) (2020)
   PUBMED   32436936
  REMARK    GeneRIF: Involvement of amylin B-H2S-connexin 43 signaling pathway
            in vascular dysfunction and enhanced ischemia-reperfusion-induced
            myocardial injury in diabetic rats.
REFERENCE   5  (bases 1 to 2345)
  AUTHORS   Bharadwaj,P., Solomon,T., Sahoo,B.R., Ignasiak,K., Gaskin,S.,
            Rowles,J., Verdile,G., Howard,M.J., Bond,C.S., Ramamoorthy,A.,
            Martins,R.N. and Newsholme,P.
  TITLE     Amylin and beta amyloid proteins interact to form amorphous
            heterocomplexes with enhanced toxicity in neuronal cells
  JOURNAL   Sci Rep 10 (1), 10356 (2020)
   PUBMED   32587390
  REMARK    GeneRIF: Amylin and beta amyloid proteins interact to form
            amorphous heterocomplexes with enhanced toxicity in neuronal cells.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 2345)
  AUTHORS   van Mansfeld,A.D., Mosselman,S., Hoppener,J.W., Zandberg,J., van
            Teeffelen,H.A., Baas,P.D., Lips,C.J. and Jansz,H.S.
  TITLE     Islet amyloid polypeptide: structure and upstream sequences of the
            IAPP gene in rat and man
  JOURNAL   Biochim Biophys Acta 1087 (2), 235-240 (1990)
   PUBMED   2223885
REFERENCE   7  (bases 1 to 2345)
  AUTHORS   Asai,J., Nakazato,M., Miyazato,M., Kangawa,K., Matsuo,H. and
            Matsukura,S.
  TITLE     Regional distribution and molecular forms of rat islet amyloid
            polypeptide
  JOURNAL   Biochem Biophys Res Commun 169 (2), 788-795 (1990)
   PUBMED   2357234
REFERENCE   8  (bases 1 to 2345)
  AUTHORS   Betsholtz,C., Christmansson,L., Engstrom,U., Rorsman,F.,
            Svensson,V., Johnson,K.H. and Westermark,P.
  TITLE     Sequence divergence in a specific region of islet amyloid
            polypeptide (IAPP) explains differences in islet amyloid formation
            between species
  JOURNAL   FEBS Lett 251 (1-2), 261-264 (1989)
   PUBMED   2666169
REFERENCE   9  (bases 1 to 2345)
  AUTHORS   Leffert,J.D., Newgard,C.B., Okamoto,H., Milburn,J.L. and
            Luskey,K.L.
  TITLE     Rat amylin: cloning and tissue-specific expression in pancreatic
            islets
  JOURNAL   Proc Natl Acad Sci U S A 86 (9), 3127-3130 (1989)
   PUBMED   2654937
REFERENCE   10 (bases 1 to 2345)
  AUTHORS   Clark,A., Cooper,G.J., Lewis,C.E., Morris,J.F., Willis,A.C.,
            Reid,K.B. and Turner,R.C.
  TITLE     Islet amyloid formed from diabetes-associated peptide may be
            pathogenic in type-2 diabetes
  JOURNAL   Lancet 2 (8553), 231-234 (1987)
   PUBMED   2441214
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000004.1.
            
            On or before Oct 5, 2022 this sequence version replaced
            XM_006237614.4, NM_012586.2.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: J04544.1, SRR23984936.847082.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN16676784, SAMN16676785
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-83                JAXUCZ010000004.1  176968097-176968179
            84-178              JAXUCZ010000004.1  176968468-176968562
            179-2345            JAXUCZ010000004.1  176973197-176975363
FEATURES             Location/Qualifiers
     source          1..2345
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="4"
                     /map="4q44"
     gene            1..2345
                     /gene="Iapp"
                     /note="islet amyloid polypeptide"
                     /db_xref="GeneID:24476"
                     /db_xref="RGD:2854"
     exon            1..83
                     /gene="Iapp"
                     /inference="alignment:Splign:2.1.0"
     exon            84..178
                     /gene="Iapp"
                     /inference="alignment:Splign:2.1.0"
     CDS             96..377
                     /gene="Iapp"
                     /note="amylin; DAP; diabetes-associated peptide"
                     /codon_start=1
                     /product="islet amyloid polypeptide preproprotein"
                     /protein_id="NP_036718.1"
                     /db_xref="GeneID:24476"
                     /db_xref="RGD:2854"
                     /translation="MRCISRLPAVLLILSVALGHLRATPVGSGTNPQVDKRKCNTATC
                     ATQRLANFLVRSSNNLGPVLPPTNVGSNTYGKRNVAEDPNRESLDFLLL"
     exon            179..2345
                     /gene="Iapp"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gtgcgtgcac gcttgggctg tagttcctga agcttcaggc tgccagcaca ctatctgtta
       61 ttgctgccac tgcccactga aagggatctt gagacatgag gtgcatctcc aggctgccag
      121 ctgttctcct catcctctcg gtggcactcg gccacttgag agctacacct gtcggaagtg
      181 gtaccaaccc tcaggtggac aaacggaagt gcaacacagc cacatgtgcc acacaacgtc
      241 tggcaaactt cttggttcgc tccagcaaca accttggtcc agtcctccca ccaaccaatg
      301 tgggatccaa tacatatggg aagaggaatg tggcagagga tccaaatagg gaatccctgg
      361 atttcttact cctgtaaagt caatgtactc ccgtatctct tattacttcc tgtgtaaatg
      421 ctctgatgat ttcctgaata atgtaacagt gcctttgcca ttcccgccca agtgagaatg
      481 tacaacaacg tgcctgtgct tgctgtgttt gtaaattctt attctaagac gtgctttaaa
      541 ctgagtgttg ataaaggtca gggtgaatac ctctctaatc acaacatgtt cttggctgta
      601 catcgatatc gtaggaacac ttaaaatttc tgtttttacc ttgtaactct atgactcaag
      661 tttaacaata aaggagggcg tgggatggtg gacttgaaaa gtcattaaca gctcatagta
      721 aatttctgat tctagacatt ggaaagcaag cattctaaat acctgctaag atgacatttc
      781 aaaataaatt agcaaaatct tgtacttatt taattaaact ttttaattct atttcttgtt
      841 gtccttgttg gtagtaagag gctgttttaa aggataaagg ttgtttgtat ccacgaggag
      901 tttattgttt ctttttctct gtagagagct ttaatgacat gtttattctg ctaaggaatc
      961 tttcagcaaa actcaaacat aattgtatcc agcagaagga tcctgtcttc tattctgaat
     1021 gccttaaaga tttgtggaaa ctagaaacca aacctgtatg ccaacttatt ctcttggctc
     1081 agtcgtggac aaatagaaga atggcatgac tgtcgtctgc acacgtgcgt gatatgtttg
     1141 atggacatgt gggggcacat atgtgctgaa gtatgtatgt gggggtcaga gtacaatact
     1201 cagtgtcagt cttcgccatc tacctgtgtg actggttctt tgtttgttgg tcccctagcc
     1261 agaaatgcca ggcttccagg gattctccta ctcctccacc catctcacca taggagttct
     1321 gggattatag aagccactgt ccgtttccac atgggtcctg aggactcacg ctcaggcctt
     1381 cgcgctgtgc accacgcgct gtacacgctg acttccaacg actttcccat gggatctcag
     1441 gtcacaggtt catcacagct acacgctgac ttccaacgac tttcccatag gatctcaggt
     1501 cacaggttca tcacagctac acgctgactt ccaacgactt tcccatggga tctcaggtca
     1561 caggttcatc acagctacac gctgacttcc aacgactttc ccatgggatc tcaggtcaca
     1621 ggttcatcac agctacacgc tgacttccaa cgactttccc atgggatctc aggtcacagg
     1681 ttcatcacag ctactcatgg gcgttgggca tctgacttct gtccactgca cacttcacac
     1741 ttagcatacc tccacgcctg gctatgtact ctggtcactc ccaacagcta ccgtcacctc
     1801 tgggatgtgc tcagttctgt gcaaatggtg catactctgt gtgcagagcc tctcccagtc
     1861 ctctccgatt cctctgaatt ctgagatatc cacattactc ttccgacacc cgttggctca
     1921 aacactccct cggttactga caactgtgtg tgtgtaactt ttgtatctga taccatcatg
     1981 gtgattatga cctcaaaact accttggaga agctcaacat acagttttct aacatacagt
     2041 tttgtcacca tgctcatttg tctgcctgtc tgtccggtaa gctccctgag gtcagagttg
     2101 tatggtcgct atctatgctc ctcgcactac ttcagatgat tgttttaaca aaacaggtgt
     2161 tcggtgcata catttggaat cactgtcatc tgcggacgag caaatgcaat gggctttggg
     2221 ataacaataa ttctgtagag tgcaatgagg ttttaaaaga gtgtagcttt ttacattctc
     2281 tttataaaac aaagatagcg atgccttcct tgaatagttt tattaaaggg tagaactaac
     2341 ctaaa
//
