LOCUS       NM_001109092            4580 bp    mRNA    linear   ROD 01-APR-2024
DEFINITION  Rattus norvegicus HUS1 checkpoint clamp component (Hus1),
            transcript variant 1, mRNA.
ACCESSION   NM_001109092 XM_001054510 XM_006251468 XM_573658
VERSION     NM_001109092.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 4580)
  AUTHORS   Cotta-Ramusino,C., McDonald,E.R. 3rd, Hurov,K., Sowa,M.E.,
            Harper,J.W. and Elledge,S.J.
  TITLE     A DNA damage response screen identifies RHINO, a 9-1-1 and TopBP1
            interacting protein required for ATR signaling
  JOURNAL   Science 332 (6035), 1313-1317 (2011)
   PUBMED   21659603
REFERENCE   2  (bases 1 to 4580)
  AUTHORS   Wang,X., Hu,B., Weiss,R.S. and Wang,Y.
  TITLE     The effect of Hus1 on ionizing radiation sensitivity is associated
            with homologous recombination repair but is independent of
            nonhomologous end-joining
  JOURNAL   Oncogene 25 (13), 1980-1983 (2006)
   PUBMED   16278671
  REMARK    Erratum:[Oncogene. 2008 Feb 21;27(9):1343]
REFERENCE   3  (bases 1 to 4580)
  AUTHORS   Levitt,P.S., Liu,H., Manning,C. and Weiss,R.S.
  TITLE     Conditional inactivation of the mouse Hus1 cell cycle checkpoint
            gene
  JOURNAL   Genomics 86 (2), 212-224 (2005)
   PUBMED   15919177
REFERENCE   4  (bases 1 to 4580)
  AUTHORS   Jiang,K., Pereira,E., Maxfield,M., Russell,B., Goudelock,D.M. and
            Sanchez,Y.
  TITLE     Regulation of Chk1 includes chromatin association and 14-3-3
            binding following phosphorylation on Ser-345
  JOURNAL   J Biol Chem 278 (27), 25207-25217 (2003)
   PUBMED   12676962
REFERENCE   5  (bases 1 to 4580)
  AUTHORS   Weiss,R.S., Leder,P. and Vaziri,C.
  TITLE     Critical role for mouse Hus1 in an S-phase DNA damage cell cycle
            checkpoint
  JOURNAL   Mol Cell Biol 23 (3), 791-803 (2003)
   PUBMED   12529385
REFERENCE   6  (bases 1 to 4580)
  AUTHORS   Weiss,R.S., Matsuoka,S., Elledge,S.J. and Leder,P.
  TITLE     Hus1 acts upstream of chk1 in a mammalian DNA damage response
            pathway
  JOURNAL   Curr Biol 12 (1), 73-77 (2002)
   PUBMED   11790307
REFERENCE   7  (bases 1 to 4580)
  AUTHORS   Cai,R.L., Yan-Neale,Y., Cueto,M.A., Xu,H. and Cohen,D.
  TITLE     HDAC1, a histone deacetylase, forms a complex with Hus1 and Rad9,
            two G2/M checkpoint Rad proteins
  JOURNAL   J Biol Chem 275 (36), 27909-27916 (2000)
   PUBMED   10846170
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000014.1.
            
            On or before May 5, 2021 this sequence version replaced
            XM_006251468.2, NM_001109092.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR26360190.1103088.1,
                                           SRR26643293.58027.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA5760383,
                                           SAMEA5760386 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-70                JAXUCZ010000014.1  87917065-87917134   c
            71-198              JAXUCZ010000014.1  87916510-87916637   c
            199-375             JAXUCZ010000014.1  87916253-87916429   c
            376-483             JAXUCZ010000014.1  87914255-87914362   c
            484-558             JAXUCZ010000014.1  87913175-87913249   c
            559-658             JAXUCZ010000014.1  87908601-87908700   c
            659-778             JAXUCZ010000014.1  87907796-87907915   c
            779-4580            JAXUCZ010000014.1  87902458-87906259   c
FEATURES             Location/Qualifiers
     source          1..4580
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="14"
                     /map="14q21"
     gene            1..4580
                     /gene="Hus1"
                     /note="HUS1 checkpoint clamp component"
                     /db_xref="GeneID:498411"
                     /db_xref="RGD:1591976"
     exon            1..70
                     /gene="Hus1"
                     /inference="alignment:Splign:2.1.0"
     CDS             19..861
                     /gene="Hus1"
                     /note="isoform 1 is encoded by transcript variant 1; Hus1
                     homolog; checkpoint protein HUS1; HUS1 checkpoint homolog"
                     /codon_start=1
                     /product="checkpoint protein HUS1 isoform 1"
                     /protein_id="NP_001102562.1"
                     /db_xref="GeneID:498411"
                     /db_xref="RGD:1591976"
                     /translation="MKFRAKIVDLACLNHFTRVSNMIAKLAKTCTLRISPEKLNFILC
                     DKLASGGVSMWCELEQENFFSEFQMEGVSEENNEIYLELTSENLARALKTAQNARALK
                     IKLTNKHFPCLTVSVELLSSSSSSRIVVHDIPIKVIPRRLWKDLQEPSIPESDVSIYL
                     PALKTMKSVVDKMRNISNHLVIEANLKGELNLKIETESVCVTTHFKDLENPLLPSDSV
                     SQNRHPEDMAKVHIDIRKLLQLLAGQQVAPTKAVCNIVNNRTIHFDLLLEDVSLQYFI
                     PALS"
     exon            71..198
                     /gene="Hus1"
                     /inference="alignment:Splign:2.1.0"
     exon            199..375
                     /gene="Hus1"
                     /inference="alignment:Splign:2.1.0"
     exon            376..483
                     /gene="Hus1"
                     /inference="alignment:Splign:2.1.0"
     exon            484..558
                     /gene="Hus1"
                     /inference="alignment:Splign:2.1.0"
     exon            559..658
                     /gene="Hus1"
                     /inference="alignment:Splign:2.1.0"
     exon            659..778
                     /gene="Hus1"
                     /inference="alignment:Splign:2.1.0"
     exon            779..4580
                     /gene="Hus1"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 ctcggcctgg tatccgccat gaagtttcgt gccaagatcg tggatctggc ttgtctgaat
       61 cactttacac gagtcagtaa catgatagcc aagcttgcca aaacctgcac cctccgcatc
      121 agcccagaga agctgaactt catcctctgt gacaagctgg ccagtggagg cgtgagcatg
      181 tggtgtgagc tggagcagga gaactttttt agtgaattcc aaatggaagg agtctctgaa
      241 gaaaacaatg agatttattt agaattaacg tcggaaaact tagctcgagc cttgaagact
      301 gcccagaacg ccagagcctt gaaaatcaag cttactaaca agcactttcc ctgtctaacc
      361 gtgtccgtag agctgttgtc ttcatcaagc agtagcagaa ttgtggtaca tgatatcccc
      421 ataaaggtta ttccgagaag actgtggaag gacttacaag aaccctccat cccagaatct
      481 gatgtcagta tttatctacc agccttgaag acaatgaaga gtgtcgtgga taaaatgagg
      541 aacatcagca atcaccttgt tattgaagca aacctaaaag gagaactaaa cctaaagata
      601 gaaactgagt cagtatgtgt gacaacacac tttaaggatc ttgaaaaccc tctactacct
      661 tctgacagtg tctctcaaaa cagacaccca gaagacatgg ccaaggtgca catcgacata
      721 aggaaactcc tccagttgct tgctggacag caagtggctc ccaccaaggc ggtgtgcaat
      781 atcgtcaata acagaactat tcactttgat ctgctcctgg aagacgtctc ccttcagtat
      841 ttcatcccag ccttgtccta gcaccatctc agctgaccca ggaggacaga ggactttgtc
      901 gtgatgagag aagcactgga cgatatggtc agtcagctgc ctgtcctcac agtaccacac
      961 atttgaactg ggcttatttc tctccctatg ctgctttaaa cacaaatgga agtcgtgaag
     1021 cacagtcgga tagtcattcc ttcatatgac tcagtaccaa ccccttcgcc ctttaagcca
     1081 ttaagaagaa aagagaattt attttttgta gggtttttgt tgttttgttt tcttttggtg
     1141 tgggcgatga gaccagggcc tcgtgtgctg agcgcatgct ctaccactga gctgccccca
     1201 ttcctatcga caatctgagt agcagtttgt cactacagtt gcaaaagtta tcatatgtgt
     1261 aaaattcatg ttgcagtcat aattcacagg agcgccaccc aggtagcttc cagctccttc
     1321 cctggtatag cacagctgtc tgctttcaga ttttactgtg ctcatttctc ccacccacac
     1381 atctggatgc tcagtacaaa atggacggcc cctctgtccc tgcagcgtgc ccggtttctc
     1441 accacactga gtatacacct gcagcatgtg gagccttgca agattactga agattcgagt
     1501 ccaccgactt ccctctcacg gcctgcagag cctcccagcc cggacaaatg ggtccctgag
     1561 agtctaaagg acccagaaac cctaccgcac acagttccca tcttgaactt gtccgttttg
     1621 tactttctgc ctgagcccac tttggcttta ggttataact tacttccctg ttctgtagga
     1681 ctcaggctcc agcccaggac taggtacatg ctaggcaagc gctctgtgcc aaactgtacc
     1741 tccagtcctt tattccacaa ttttgcataa atctttccaa gtgtcaggtc tacccttcta
     1801 taaaaccttg tgtttgcagt ctgagcttgg ctgtcacatt tgaagcgatg acgtatgtct
     1861 gctcttgaat tggagatggc aatgtgattt cctattctct aaagacaccg ttcgcaggtt
     1921 tcaggtgtcc gtgaggtggc atcataaaca attttcatgt cagaaagtca agacctaagt
     1981 tgcctagggt aagatttaaa aagaaaaaag aaaagtaaac aaaaagaaca tttctttttt
     2041 ggctttttca aattatttag taaaactatt tctcaacagc tgttaggatt attcttcata
     2101 gtttccattt ttatccgaaa ggttctgtgt aagtcaagct ggactcagat gaactgtaca
     2161 tagattggcc tagaactcct gagcctccca cctcctgctc ccaagtgttg ccatgacaga
     2221 tatgagccat cataccactg gctcttctgc tgtttaataa gatatgttta aactacattt
     2281 taaaactagg gattcttaat tgttaaagct cataacaaaa atcagtcaaa atcatgatgc
     2341 agctagaatt tcaggacact ccctatacct tgttccttgg gcttgtcttt tttgccggaa
     2401 gtaccagaac tagtcactaa tttcaccgct gcaccgcata ccggatgtgg tttactttac
     2461 agtgacccaa cgtggggaag aagaagcttc cactggcagc caagggctgt ggtctttctg
     2521 gttctggtca caactttctg catggtgatc ctttcatagc ttatgtctca aggagctgtg
     2581 gtcaagggag ggagctgaga aagtgctggg ctgtgggacc tcgaagctgc tttgtggagc
     2641 tccatcctga agtgggggca gactggagac attttaccgt gtgttgaagg agcaagttgt
     2701 ctgaggtttg gagacattca cttactgtga ctgcctaagg ctctgaggtt tggagagggt
     2761 ttccatatat atgcaagcca ctaagaggac cccccaacac acacacctgc cagggctcag
     2821 aacccacatc ccggtagcca tctatgcctt gagctgaggt tttacgactg tcctgagaca
     2881 gtcaggagtg caggcagaat tcctaatgca gtcacttatg ccaatgtccg agtgggcttg
     2941 ttgtcctgtg gccttccctt gtccagcagg caccttccta cccagcagct ctccacactg
     3001 cattggtttg tcctgctgtg catcctgcca caaagaatcc tgcccttgga agcctgaaaa
     3061 gcaattcagc attagttctg ggcaggaatt ccagcccttc caccacgtgc agtgtccgat
     3121 gatattctag gtgaatgtca cccaattagt ggagtatcca ttgcttttaa gtcatacagt
     3181 tctgtacttt gtaactttag atttcgctgg ggtatcagag tgtgcaagct ggaggattgt
     3241 tagatggttc agagttctac agctgccact ccaccccacc acttttcact ccacctatcc
     3301 tcctgggcac ctcgttattt actctcagtt tctatacttg cctgccgtgg acagcttttt
     3361 aacctcctcc cctggtgccc aggcctctgg aaccaccatt ccttactata gccatgaggg
     3421 ccccagtgct cttgactctg gttctattct cactatctgc ctatcaaatg tatactgatc
     3481 ctagccttgc accttggctt ttgcccatgt ccatccttgc acagtaaatc agtctagaag
     3541 ttctgtattc ctgtaggcac tgcaccattg caaagctcga tggcattgcc atttctacaa
     3601 gggtgctctc ccggcattca tactttgatt gctgggaggc gcattgcatt ttatatttaa
     3661 atggccatgg gtaggctagc tctgctctgc ttgcactgtc acatgtgaac agaccagacc
     3721 aaccagactc ctacaatgtc tcagaatatg gggaagccaa atgctagctc ctgtctgttt
     3781 tttccatgtg gaatcaatac agggattgat gtggtgttat gagctgggta gagcagcttt
     3841 gtaaactccc tgactgtctg ctcatggttt tgcatttctc tgtggcccag tgggtgtcgc
     3901 agcatcactc tggagtttgg agaattcagg gtggtcatct tgcggctgga aggttgctag
     3961 ttggaaatat gtgaggacct gaagaagatt gcgttccacc attttgctaa tgccacactc
     4021 cagttctgtg ctgctgtgtg gattctgaca gtgagtctaa agggagcttg agttcattgg
     4081 ttctcttcct aagaacagtg ttcaactagg ggatgacaga ttggagtcac ttggacatcg
     4141 atgataggag ggcattagca ccactgtcct gaagcctcct gactgcttac tctctgtggg
     4201 aaagctcaaa accactccct ctcatttaat acctgtgccc atgagattca ttccacaggc
     4261 tacagtcaat gcttggtact gttataaata aggtttccta atgtgaatga aagggaattg
     4321 gtagttgatc tatgctgtga tataatatcg taccctaatg cgatatgata aagtgctgct
     4381 tacatggtaa caaatccagt gggctatcgg tgtccacttg tgacattaaa gaccagaatc
     4441 atacatctaa atccacttat ctctgggtgt tcttgacttc agtcttttta catttgtgta
     4501 ttttctaaca aattcatgac caacctttta atgaaaatat ttttatagaa caaaataaat
     4561 atttataatt taactcttaa
//
