LOCUS       NM_012853               4575 bp    mRNA    linear   ROD 05-OCT-2024
DEFINITION  Rattus norvegicus 5-hydroxytryptamine receptor 4 (Htr4), mRNA.
ACCESSION   NM_012853 XM_008772089
VERSION     NM_012853.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 4575)
  AUTHORS   Lopez,E.R., Carbajal,A.G., Tian,J.B., Bavencoffe,A., Zhu,M.X.,
            Dessauer,C.W. and Walters,E.T.
  TITLE     Serotonin enhances depolarizing spontaneous fluctuations,
            excitability, and ongoing activity in isolated rat DRG neurons via
            5-HT4 receptors and cAMP-dependent mechanisms
  JOURNAL   Neuropharmacology 184, 108408 (2021)
   PUBMED   33220305
  REMARK    GeneRIF: Serotonin enhances depolarizing spontaneous fluctuations,
            excitability, and ongoing activity in isolated rat DRG neurons via
            5-HT4 receptors and cAMP-dependent mechanisms.
REFERENCE   2  (bases 1 to 4575)
  AUTHORS   Guo,Y., Zhang,L., Zhang,J., Du,C.X., Lv,S.X., Wang,T., Wang,H.S.,
            Xie,W. and Liu,J.
  TITLE     Activation and blockade of serotonin4 receptors in the lateral
            habenula improve working memory in unilateral
            6-hydroxydopamine-lesioned Parkinson's rats
  JOURNAL   Neurol Res 41 (7), 585-593 (2019)
   PUBMED   30929589
  REMARK    GeneRIF: findings suggested that unilateral lesions of the
            Substantia Nigra induced working memory impairment and
            up-regulation of 5-HT4 receptors in the Lateral Habenula.
            Activation and blockade of LHb 5-HT4 receptors improved working
            memory, that were related to the change of monoamines levels.
REFERENCE   3  (bases 1 to 4575)
  AUTHORS   Garcia-Pedraza,J.A., Garcia-Domingo,M., Gomez-Roso,M.,
            Ruiz-Remolina,L., Rodriguez-Barbero,A., Martin,M.L. and Moran,A.
  TITLE     Hypertension exhibits 5-HT4 receptor as a modulator of sympathetic
            neurotransmission in the rat mesenteric vasculature
  JOURNAL   Hypertens Res 42 (5), 618-627 (2019)
   PUBMED   30696976
  REMARK    GeneRIF: Hypertension exhibits 5-HT4 receptor as a modulator of
            sympathetic neurotransmission in the rat mesenteric vasculature.
REFERENCE   4  (bases 1 to 4575)
  AUTHORS   Lyubashina,O.A. and Sivachenko,I.B.
  TITLE     The 5-HT4 receptor-mediated inhibition of visceral nociceptive
            neurons in the rat caudal ventrolateral medulla
  JOURNAL   Neuroscience 359, 277-288 (2017)
   PUBMED   28754313
  REMARK    GeneRIF: Results indicate that visceral nociceptive transmission
            through the caudal medulla is negatively modulated by descending
            serotonin type 4 receptor dependent mechanisms.
REFERENCE   5  (bases 1 to 4575)
  AUTHORS   Kozono,N., Ohtani,A. and Shiga,T.
  TITLE     Roles of the serotonin 5-HT4 receptor in dendrite formation of the
            rat hippocampal neurons in vitro
  JOURNAL   Brain Res 1655, 114-121 (2017)
   PUBMED   27894797
  REMARK    GeneRIF: Study suggests a novel role for the 5-HT4 receptor in
            facilitation of dendrite formation in which intracellular signaling
            of PKA and the BDNF-TrkB system may be involved.
REFERENCE   6  (bases 1 to 4575)
  AUTHORS   Manzke,T., Guenther,U., Ponimaskin,E.G., Haller,M., Dutschmann,M.,
            Schwarzacher,S. and Richter,D.W.
  TITLE     5-HT4(a) receptors avert opioid-induced breathing depression
            without loss of analgesia
  JOURNAL   Science 301 (5630), 226-229 (2003)
   PUBMED   12855812
  REMARK    GeneRIF: results report that serotonin 4(a) receptors are strongly
            expressed in respiratory Pre-Boetzinger complex neurons and that
            their selective activation protects spontaneous respiratory
            activity
REFERENCE   7  (bases 1 to 4575)
  AUTHORS   Cai,X., Flores-Hernandez,J., Feng,J. and Yan,Z.
  TITLE     Activity-dependent bidirectional regulation of GABA(A) receptor
            channels by the 5-HT(4) receptor-mediated signalling in rat
            prefrontal cortical pyramidal neurons
  JOURNAL   J Physiol 540 (Pt 3), 743-759 (2002)
   PUBMED   11986365
  REMARK    GeneRIF: results suggest that 5-HT(4) receptors can modulate
            GABAergic signalling bidirectionally, depending on the basal PKA
            activation levels that are determined by neuronal activity
REFERENCE   8  (bases 1 to 4575)
  AUTHORS   Claeysen,S., Sebben,M., Becamel,C., Bockaert,J. and Dumuis,A.
  TITLE     Novel brain-specific 5-HT4 receptor splice variants show marked
            constitutive activity: role of the C-terminal intracellular domain
  JOURNAL   Mol Pharmacol 55 (5), 910-920 (1999)
   PUBMED   10220570
REFERENCE   9  (bases 1 to 4575)
  AUTHORS   Ullmer,C., Schmuck,K., Kalkman,H.O. and Lubbert,H.
  TITLE     Expression of serotonin receptor mRNAs in blood vessels
  JOURNAL   FEBS Lett 370 (3), 215-221 (1995)
   PUBMED   7656980
REFERENCE   10 (bases 1 to 4575)
  AUTHORS   Gerald,C., Adham,N., Kao,H.T., Olsen,M.A., Laz,T.M.,
            Schechter,L.E., Bard,J.A., Vaysse,P.J., Hartig,P.R., Branchek,T.A.
            et al.
  TITLE     The 5-HT4 receptor: molecular cloning and pharmacological
            characterization of two splice variants
  JOURNAL   EMBO J 14 (12), 2806-2815 (1995)
   PUBMED   7796807
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000018.1.
            
            On or before Dec 1, 2020 this sequence version replaced
            XM_008772089.2, NM_012853.1.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR26643299.63028.1,
                                           SRR26360194.959669.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760433, SAMN14984024
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-796               JAXUCZ010000018.1  58036277-58037072
            797-869             JAXUCZ010000018.1  58053533-58053605
            870-995             JAXUCZ010000018.1  58127051-58127176
            996-1196            JAXUCZ010000018.1  58128377-58128577
            1197-1350           JAXUCZ010000018.1  58143289-58143442
            1351-1919           JAXUCZ010000018.1  58155087-58155655
            1920-4575           JAXUCZ010000018.1  58178612-58181267
FEATURES             Location/Qualifiers
     source          1..4575
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="18"
                     /map="18q12.1"
     gene            1..4575
                     /gene="Htr4"
                     /note="5-hydroxytryptamine receptor 4"
                     /db_xref="GeneID:25324"
                     /db_xref="RGD:2850"
     exon            1..796
                     /gene="Htr4"
                     /inference="alignment:Splign:2.1.0"
     exon            797..869
                     /gene="Htr4"
                     /inference="alignment:Splign:2.1.0"
     CDS             844..2010
                     /gene="Htr4"
                     /note="5-HT-4; 5-hydroxytryptamine (serotonin) receptor 4,
                     G protein-coupled; serotonin receptor 4; 5-HT4 receptor
                     (two splicing variants L and S); 5 hydroxytryptamine
                     (serotonin) receptor 4"
                     /codon_start=1
                     /product="5-hydroxytryptamine receptor 4"
                     /protein_id="NP_036985.2"
                     /db_xref="GeneID:25324"
                     /db_xref="RGD:2850"
                     /translation="MDRLDANVSSNEGFGSVEKVVLLTFFAMVILMAILGNLLVMVAV
                     CRDRQLRKIKTNYFIVSLAFADLLVSVLVMPFGAIELVQDIWFYGEMFCLVRTSLDVL
                     LTTASIFHLCCISLDRYYAICCQPLVYRNKMTPLRIALMLGGCWVIPMFISFLPIMQG
                     WNNIGIVDVIEKRKFNHNSNSTFCVFMVNKPYAITCSVVAFYIPFLLMVLAYYRIYVT
                     AKEHAQQIQMLQRAGATSESRPQTADQHSTHRMRTETKAAKTLCVIMGCFCFCWAPFF
                     VTNIVDPFIDYTVPEKVWTAFLWLGYINSGLNPFLYAFLNKSFRRAFLIILCCDDERY
                     KRPPILGQTVPCSTTTINGSTHVLRDTVECGGQWESRCHLTATSPLVAAQPSDT"
     exon            870..995
                     /gene="Htr4"
                     /inference="alignment:Splign:2.1.0"
     exon            996..1196
                     /gene="Htr4"
                     /inference="alignment:Splign:2.1.0"
     exon            1197..1350
                     /gene="Htr4"
                     /inference="alignment:Splign:2.1.0"
     exon            1351..1919
                     /gene="Htr4"
                     /inference="alignment:Splign:2.1.0"
     exon            1920..4575
                     /gene="Htr4"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 tgatcagatt tgttgacagc agatggattt tgcgctgtca tcggccctca cagacacttc
       61 agatccctga gtggtaccca cgcctggacg ttcggaagcg tgtaacacta ctatcagttt
      121 gtaccctgcc ttccatgtga gagcactgag gccagttcct gtctagtcac gtggtcaagg
      181 tcacaggtgg tcagagctgg gagaaccagc tcactaactt cagataagat ggcaaagagc
      241 agactcacaa ggatggggct gatcgcttag ggagccctca aacatctgaa agcattaagt
      301 ctgcagaact tgtgctccaa ttcccgaagt gccaggtact cccttgaagg ccactcccag
      361 ccaaaccttg ctctgggcca ggcggtgtca ggcatgagaa ggaggaagta gactgcacaa
      421 aaggattgcc tctggcacag acacctcaga gcagtctgga gtttattgtc tctgaataga
      481 ggaagtggac actcaaagag gtgaacagat ttgcccaagg tcaccgagga gtctgcagtg
      541 aggccagggt ttgccccctg tccccagcga atgggtacgg tggtcctggt cagagagagc
      601 acatgctggc cagcccaggg gtgtgagatc gggcaatctc tgtgcccact ccccagtgcg
      661 cccctctgca caggtggcac cccgcgtgcc ctgtgggggc ggcagcgagc cggaccgcag
      721 ggacacctaa gctgggggct gtggagcctg ccgagcctgg cttggttgga aggaggagga
      781 tgctctgcgt gcccagggtc ctgtgggcac tgacatccaa cgtactcatg cccatttcct
      841 gtaatggaca gacttgatgc taatgtgagt tccaacgagg gtttcgggtc tgtggagaag
      901 gtcgtactgc tcacgttctt cgcaatggtt atcctgatgg ccatcctggg caacctgctg
      961 gtgatggttg ctgtgtgcag ggacaggcag ctcaggaaaa taaaaaccaa ttatttcatt
     1021 gtgtctcttg cctttgctga tctgctggtt tcggtgctgg tgatgccctt cggtgccatt
     1081 gagttggtcc aagacatctg gttttatggg gagatgtttt gcctggtccg gacctctctg
     1141 gatgtcctac tcaccacagc atcaattttt cacctctgct gcatttccct ggataggtat
     1201 tatgccatct gctgtcaacc tttggtttat agaaacaaga tgacccctct acgcatcgca
     1261 ttaatgctgg gaggctgctg ggtcattccc atgtttatat cttttctccc cataatgcaa
     1321 ggctggaaca acatcggcat agttgatgtg atagagaaaa ggaaattcaa ccacaactct
     1381 aactctacat tctgtgtctt catggtcaac aagccctatg ccatcacctg ctctgtggtg
     1441 gccttctaca tcccgtttct cctcatggtg ctggcctatt accgtatcta tgtcactgct
     1501 aaggagcatg cccagcagat ccagatgtta caacgggcag gagccacctc tgaaagcagg
     1561 ccccagacag ctgaccagca cagcacacat cgcatgcgga cagagaccaa agcagccaag
     1621 actttatgtg tcatcatggg ctgcttctgt ttctgctggg cccccttctt tgtcaccaat
     1681 attgtggacc ctttcataga ctacactgtg cccgagaagg tgtggactgc tttcctctgg
     1741 cttggctata tcaattcagg gttgaaccct tttctctatg ccttcttgaa taagtctttc
     1801 agacgtgcct tccttatcat cctctgctgt gatgatgagc gctacaaaag accccccatt
     1861 ctgggccaga ctgtcccctg ttcaaccaca accattaatg gatccactca tgtgctaagg
     1921 gatacagtgg aatgtggtgg ccaatgggag agtcggtgtc acctcacagc aacttctcct
     1981 ttggtggctg ctcagcccag tgatacgtag gccccaggac aatgacctag aagacagctg
     2041 tagcttgaaa agaagccagt cctaagctgc tacttcggtg tatgtggctg cccctggcac
     2101 tttgttctcc aaggctttcc aagagcatga ggcaatccac cctggacttc ccgccacgat
     2161 tctagcaggc ggtattagag gaagtcaggg gagagaaggg cttcctcctt agctttctgt
     2221 ttctcaacat tttctcttcc tggagtctcc actcttgctt ggtggtctct gaagtccacg
     2281 acccagtccc cttgctgtct ccagtctgtc ttgtaaatgt ttaccgtgtt cgattttcag
     2341 tttccaaaca tgccttcttt gaagtgtcat cttacggata ctgtcaaaac atgtgcctgt
     2401 cttgatcaca cttcttgctt ggtcctattt accatagctg tgtttaaact ctctaccgtc
     2461 ctttctctgc tttctgctgt gcacttggac ccttttgtcc ctttgtctag gtgtagacgt
     2521 gcatcctttt ggccagtcct gtgtcaacca tggagctctg ttttctttta cctgtgttta
     2581 gtgctaatgt cctcttgagg acaagctcca ctcacttgcc attggagaca cagaagagag
     2641 atctgtcagt agaaaatggt ctctgcagaa accctggagg gaagtcaaag acagtttccc
     2701 ccagtgccag gaccagatct atctcatctg agagatttga ttttcctttc agcatagata
     2761 gaacacctaa agagccttag gcggggctgg tgtcatcatt aagagatgct cagattttcc
     2821 tatgagcttg tggtgaaacc cacagtagca gctcgaaacc tcagactatg gctttgctgc
     2881 ttccaatgga cagaaccaat ggatgctcac cttgtggagc cttgataccc ttgctatctt
     2941 tcagatggag aactacagag tccagaaacg ctagtaatga aaaaaacagt atctgactcc
     3001 aagtcagagg acccaaatgt cagcccagac cctgcttcta aaatgctgtg agaccatgaa
     3061 cgatgtcttt ccatctatga atttcacttt ctatgttgcc aaacgaaact tggagtaggg
     3121 gagtctttaa gatttctttc catttctaag acaataggat gctgctatag cctgccatct
     3181 gctacctcga aaactcatgg cctgcctacc tgttggcatt gtttcaaagg aatggcccta
     3241 ctgatttggg cattctagac ttctgacttg gtcatctgtg cccttacagt gaacctggtg
     3301 tcttttactc aaagcagagt ccatggtttg tcccaactac cagagagaaa aggtcctcag
     3361 caagggaagc accacttctc agaaacaggt gacagttcag ctttcacctt gactgtggtt
     3421 tgattccatt tcaggaccat gcactgcacc attctcttaa gcttctcatg gagtggtact
     3481 tgctattcct gtcagcattg actttcattc tggaactgaa cttggcagga aaagagactt
     3541 tggaatgagg aagagaagat tgccaaaacc agtcaaatgc caaaaaagaa ttctctgtta
     3601 tgtgaagact taagttctct tgagcgcaag ccttagcaaa aggaagtaga ccctgagaga
     3661 gaacttctat atgttgctga gaaaaaaaaa aaaaaaagat ctgcgctaag aaatattgca
     3721 tgctgagatg gttcacgcca aaactgaccc agtgagtggt tctcactgga ggcctcttct
     3781 tgtcaaccat gaagagagtt gcattacctt gcatactgac tctgtagcag gatcagtcca
     3841 ctttgatttg caagtggttg gttccctgcc atggataggt tcagccctca tttggaggca
     3901 agcagtgttt tgaggttggt tcttctctaa cacaaaagat gagaggcaac caatttgcct
     3961 cagctgacca aatgcccagc ctctcaatga acatagtggt gagtgagtga ctcaagcact
     4021 gagtcaatga tggactgaca agtggatgga actgaaagct ggatggtgtg agcctcagaa
     4081 acgcttccgt gtactccata actgttgatg gggagagaaa ttatgtttat ggaaacattt
     4141 tacaaaccat aaaatgacag ctcatatgct tgttatgatt atgggtattt tgaattactg
     4201 tagatgtgct taagctctca agaccatttt atttttcact acagaatgag atcagtttag
     4261 aatgagcttt ctgtgttaga aacaaaaccc aagagcagcc cttgagtacc aacatgcccc
     4321 tgggtactcc tcctcgttat ttggtcctca ttgattactc agcacacata tttctacacc
     4381 accctccatt taaatgattt ctttgttctc agagtggtga atagagaaag agactgtggg
     4441 caagttttgg taaaaatgac tctaatttta tatgcaattt cataatctca tgagctaagt
     4501 tcatatacct gtttttcata taaaaatgta tccaaaaatg ttccctgtcc ataaaattaa
     4561 ttcccacctt ataaa
//
