LOCUS       NM_012584               1949 bp    mRNA    linear   ROD 28-MAR-2024
DEFINITION  Rattus norvegicus hydroxy-delta-5-steroid dehydrogenase, 3 beta-
            and steroid delta-isomerase 5 (Hsd3b5), mRNA.
ACCESSION   NM_012584
VERSION     NM_012584.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 1949)
  AUTHORS   Yu,C., Jiang,F., Zhang,M., Luo,D., Shao,S., Zhao,J., Gao,L., Zuo,C.
            and Guan,Q.
  TITLE     HC diet inhibited testosterone synthesis by activating endoplasmic
            reticulum stress in testicular Leydig cells
  JOURNAL   J Cell Mol Med 23 (5), 3140-3150 (2019)
   PUBMED   30884106
  REMARK    GeneRIF: these steroidogenic enzymes within the Leydig cells,
            including steroidogenic acute regulatory protein (StAR),
            cholesterol side-chain cleavage cytochrome P450 (P450scc) and
            3beta-hydroxysteroid dehydrogenase (3beta-HSD), were
            down-regulated.
REFERENCE   2  (bases 1 to 1949)
  AUTHORS   Chen,Y.J., Lee,M.T., Yao,H.C., Hsiao,P.W., Ke,F.C. and Hwang,J.J.
  TITLE     Crucial role of estrogen receptor-alpha interaction with
            transcription coregulators in follicle-stimulating hormone and
            transforming growth factor beta1 up-regulation of steroidogenesis
            in rat ovarian granulosa cells
  JOURNAL   Endocrinology 149 (9), 4658-4668 (2008)
   PUBMED   18511507
  REMARK    GeneRIF: ERalpha interaction with transcription coregulators,
            histone acetylases (CBP/SRC-1), and PGC-1alpha is crucial to FSH
            and TGFbeta1-up-regulated expression of Hsd3b and Cyp11a1, and,
            thus, progesterone production in rat ovarian granulosa cells.
REFERENCE   3  (bases 1 to 1949)
  AUTHORS   Meffre,D., Delespierre,B., Gouezou,M., Schumacher,M., Stein,D.G.
            and Guennoun,R.
  TITLE     3beta-Hydroxysteroid dehydrogenase/5-ene-4-ene isomerase mRNA
            expression in rat brain: effect of pseudopregnancy and traumatic
            brain injury
  JOURNAL   J Steroid Biochem Mol Biol 104 (3-5), 293-300 (2007)
   PUBMED   17428656
  REMARK    GeneRIF: Significant decrease in the 3beta-HSD mRNA expression in
            traumatic brain injury site within the frontal cortex in both males
            and pseudopregnant females.
REFERENCE   4  (bases 1 to 1949)
  AUTHORS   Lo,M.J. and Lee,S.D.
  TITLE     Direct effects of IL-15 on corticosterone secretion by rat
            adrenocortical cells
  JOURNAL   Immunol Cell Biol 84 (1), 38-43 (2006)
   PUBMED   16405651
REFERENCE   5  (bases 1 to 1949)
  AUTHORS   Soma,K.K., Sinchak,K., Lakhter,A., Schlinger,B.A. and Micevych,P.E.
  TITLE     Neurosteroids and female reproduction: estrogen increases 3beta-HSD
            mRNA and activity in rat hypothalamus
  JOURNAL   Endocrinology 146 (10), 4386-4390 (2005)
   PUBMED   16020475
  REMARK    GeneRIF: In ovariectomized-adrenalectomized rats, estrogen
            treatment increased 3beta-HSD mRNA in the hypothalamus.
REFERENCE   6  (bases 1 to 1949)
  AUTHORS   Deng,A.Y., Dene,H. and Rapp,J.P.
  TITLE     Mapping of a quantitative trait locus for blood pressure on rat
            chromosome 2
  JOURNAL   J Clin Invest 94 (1), 431-436 (1994)
   PUBMED   8040284
REFERENCE   7  (bases 1 to 1949)
  AUTHORS   Lewin-Kowalik,J., Sieron,A.L. and Barski,J.J.
  TITLE     [Recent views on the role of nerve growth factor and other
            molecules with well documented neuronotrophic action]
  JOURNAL   Postepy Hig Med Dosw 46 (5), 497-519 (1992)
   PUBMED   1298957
  REMARK    Review article
REFERENCE   8  (bases 1 to 1949)
  AUTHORS   Zhao,H.F., Rheaume,E., Trudel,C., Couet,J., Labrie,F. and Simard,J.
  TITLE     Structure and sexual dimorphic expression of a liver-specific rat 3
            beta-hydroxysteroid dehydrogenase/isomerase
  JOURNAL   Endocrinology 127 (6), 3237-3239 (1990)
   PUBMED   2249649
REFERENCE   9  (bases 1 to 1949)
  AUTHORS   Gordon,M. and Robertson,W.R.
  TITLE     The application of continuous monitoring microdensitometry to an
            analysis of NAD+ binding and 3 beta-hydroxy-delta 5-steroid
            dehydrogenase activity in the regressing corpus luteum of the
            pro-oestrous rat ovary
  JOURNAL   Histochem J 18 (1), 41-44 (1986)
   PUBMED   3458688
REFERENCE   10 (bases 1 to 1949)
  AUTHORS   Kuhn,N.J. and Briley,M.S.
  TITLE     The roles of pregn-5-ene-3 beta, 20 alpha-diol and 20 alpha-hydroxy
            steroid dehydrogenase in the control of progesterone synthesis
            preceding parturition and lactogenesis in the rat
  JOURNAL   Biochem J 117 (2), 193-201 (1970)
   PUBMED   4392955
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from
            JAXUCZ010000002.1.
            
            On Nov 26, 2020 this sequence version replaced NM_012584.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: M67465.1, BC092571.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760476 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-34                JAXUCZ010000002.1  188716985-188717018 c
            35-229              JAXUCZ010000002.1  188708906-188709100 c
            230-394             JAXUCZ010000002.1  188701553-188701717 c
            395-1753            JAXUCZ010000002.1  188697887-188699245 c
            1754-1949           JAXUCZ010000002.1  188697671-188697866 c
FEATURES             Location/Qualifiers
     source          1..1949
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="2"
                     /map="2q34"
     gene            1..1949
                     /gene="Hsd3b5"
                     /gene_synonym="Hsd1; Hsd3b; RATHSDI"
                     /note="hydroxy-delta-5-steroid dehydrogenase, 3 beta- and
                     steroid delta-isomerase 5"
                     /db_xref="GeneID:24470"
                     /db_xref="RGD:2838"
     exon            1..34
                     /gene="Hsd3b5"
                     /gene_synonym="Hsd1; Hsd3b; RATHSDI"
                     /inference="alignment:Splign:2.1.0"
     exon            35..229
                     /gene="Hsd3b5"
                     /gene_synonym="Hsd1; Hsd3b; RATHSDI"
                     /inference="alignment:Splign:2.1.0"
     CDS             85..1206
                     /gene="Hsd3b5"
                     /gene_synonym="Hsd1; Hsd3b; RATHSDI"
                     /EC_number="1.1.1.270"
                     /EC_number="1.1.1.210"
                     /note="3 beta-hydroxysteroid dehydrogenase 5; 3
                     beta-hydroxysteroid dehydrogenase type 5; 3
                     beta-hydroxysteroid dehydrogenase type 5-like; 3-beta-HSD
                     V; progesterone reductase; 3 beta-hydroxysteroid
                     dehydrogenase type V; 3-beta-hydroxy-5-ene steroid
                     dehydrogenase; NADPH-dependent
                     3-beta-hydroxy-Delta(5)-steroid dehydrogenase; steroid
                     delta-isomerase, 3 beta; 3 beta-HSD III;
                     dihydrotestosterone 3-ketoreductase; 3 beta-hydroxysteroid
                     dehydrogenase type 3; 3 beta-hydroxysteroid dehydrogenase
                     type III"
                     /codon_start=1
                     /product="NADPH-dependent 3-keto-steroid reductase Hsd3b5"
                     /protein_id="NP_036716.2"
                     /db_xref="GeneID:24470"
                     /db_xref="RGD:2838"
                     /translation="MPGWSCLVTGAGGFLGQRIVQMLVQEKELQEVRVLYRTFSPKHK
                     EELSKLQTKAKVTVLRGDIVDAQFLRRACQGMSVIIHTAAALDIAGFLPRQTILDVNV
                     KGTQLLLDACVEASVPAFIYSSSTGVAGPNSYKETILNDREEEHRESTWSNPYPYSKR
                     MAEKAVLAANGSILKNGGTLHTCALRLPFIYGEESQIISTMVNTALKNNSIIKRHATF
                     SIANPVYVGNAAWAHILAARGLRDPEKSQSIQGQFYYISDDTPHQSYDDLNYTLSKEW
                     GFCLDSSWSLPLPLLYWLAFLLETVSFLLRPFYNYRPPFNRFMVTILNSVFTISYKKA
                     QRDLGYEPLVSWEEAKQKTSEWIGTLVEQHRETLDTKSQ"
     exon            230..394
                     /gene="Hsd3b5"
                     /gene_synonym="Hsd1; Hsd3b; RATHSDI"
                     /inference="alignment:Splign:2.1.0"
     exon            395..1949
                     /gene="Hsd3b5"
                     /gene_synonym="Hsd1; Hsd3b; RATHSDI"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 tgccttcagg gtctccaacc cttggaaaca aaacgaccac cacacatcta ctgttctctc
       61 aactatctgc ttcctgtgtt gaccatgcct gggtggagct gcctggtgac aggagcagga
      121 ggatttctgg gccagaggat tgtccaaatg ttggtgcaag agaaagagct gcaggaggtc
      181 agagtcctgt ataggacctt cagtccaaaa cacaaagagg aactgtccaa gctgcagaca
      241 aaagccaagg tgacggtact gaggggagac attgtggatg cccagttcct gaggagagcg
      301 tgccagggca tgtccgtcat catccacacc gctgctgccc ttgatatcgc aggcttcctg
      361 cccaggcaga ccatcctaga tgtcaatgtg aaaggtactc agctcctgtt ggatgcttgt
      421 gtggaagcca gcgtaccagc cttcatctac agcagctcaa caggtgtggc tggaccaaac
      481 tcctacaagg agaccatcct gaacgaccgt gaggaagagc atcgtgaaag cacatggtca
      541 aatccatacc catacagcaa aaggatggcc gagaaggcag tgctggcagc caatgggagc
      601 atcctgaaaa atggtggcac tttgcatacg tgtgccttaa gactcccatt catctatggg
      661 gaagaaagcc aaatcatttc aaccatggta aatacagcac tcaagaacaa cagcataatt
      721 aaaagacatg ccacattctc catagccaac ccagtgtatg tgggtaatgc agcctgggca
      781 cacattctgg ctgccagggg ccttcgagac cccgagaagt cacaaagcat ccaaggacag
      841 ttctactaca tctcagatga cacccctcac caaagctatg atgatttaaa ttataccctg
      901 agcaaagaat ggggcttctg ccttgattcc agttggagcc ttcctctgcc cctgctctac
      961 tggcttgcct tcctgctgga aactgtgagc ttcctgctgc gtccatttta caactatagg
     1021 ccacccttta accgcttcat ggtcacgatc ttaaatagcg tgtttaccat ttcctacaaa
     1081 aaagctcaga gagatctggg ctatgagcca cttgtcagct gggaggaagc caagcaaaaa
     1141 acctcagagt ggatcgggac actagtggag caacacaggg agacactgga cacaaagtct
     1201 caatgatggg aagagggcag ggacatggcc ccgggtgtta ccccgtcctc cagccaggaa
     1261 ctcagaaata atccaactct tacagtttta ttttatacac tctccaactg gtcttcaggt
     1321 caccagaaac cttaagagtc actggcccag tcacctgaac aaggtgcagg ggacatcatg
     1381 tagaaggaat aaaactgcct gcctcttgtg acacctgggt gttcattcac ctgccatgtg
     1441 gtatcacaaa cagaagtagg atctcagctg gcatcaaaac ttcttctttc ttacttaccc
     1501 catattaagt aatgtttata ggaagtaatg tttgtaggtg gggcttgagc tcccacacta
     1561 gagtcaggag gcaaaaccat gctgaagttc tatatagcaa accaaacctg agagtacttt
     1621 cctgatcact acaatagcaa gagagcaaga gcgagagtga gagcaagagc gagagaggga
     1681 gggaggaagg gaggaaaggg gatggaggga gggaggaaag gaaggagaga gagagagaga
     1741 gagagagaga gagcatcacc atttcacata gggtgtgaac agagtaggaa atccactttg
     1801 tttctcattt accaagagac catctggaaa gaatctgcct ttctttgccc ctgtcatacc
     1861 aagaaacctt attttcgctt attggacaat gttctatggt ataaaaaaga agagttgtct
     1921 ggttctggga ataaaactaa attccaagg
//
