LOCUS       NM_001100518            4133 bp    mRNA    linear   ROD 22-MAR-2023
DEFINITION  Rattus norvegicus heparan sulfate 2-O-sulfotransferase 1 (Hs2st1),
            mRNA.
ACCESSION   NM_001100518 XM_001079125 XM_214740
VERSION     NM_001100518.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 4133)
  AUTHORS   Berillo O, Ouerd S, Idris-Khodja N, Rehman A, Richer C, Sinnett D,
            Kwitek AE, Paradis P and Schiffrin EL.
  TITLE     Chromosome 2 Fragment Substitutions in Dahl Salt-Sensitive Rats and
            RNA Sequencing Identified Enpep and Hs2st1 as Vascular Inflammatory
            Modulators
  JOURNAL   Hypertension 77 (1), 178-189 (2021)
   PUBMED   33161775
  REMARK    GeneRIF: Chromosome 2 Fragment Substitutions in Dahl Salt-Sensitive
            Rats and RNA Sequencing Identified Enpep and Hs2st1 as Vascular
            Inflammatory Modulators.
REFERENCE   2  (bases 1 to 4133)
  AUTHORS   Ghosh D, Lippert D, Krokhin O, Cortens JP and Wilkins JA.
  TITLE     Defining the membrane proteome of NK cells
  JOURNAL   J Mass Spectrom 45 (1), 1-25 (2010)
   PUBMED   19946888
REFERENCE   3  (bases 1 to 4133)
  AUTHORS   Merry CL, Bullock SL, Swan DC, Backen AC, Lyon M, Beddington RS,
            Wilson VA and Gallagher JT.
  TITLE     The molecular phenotype of heparan sulfate in the Hs2st-/- mutant
            mouse
  JOURNAL   J Biol Chem 276 (38), 35429-35434 (2001)
   PUBMED   11457822
REFERENCE   4  (bases 1 to 4133)
  AUTHORS   Bullock SL, Fletcher JM, Beddington RS and Wilson VA.
  TITLE     Renal agenesis in mice homozygous for a gene trap mutation in the
            gene encoding heparan sulfate 2-sulfotransferase
  JOURNAL   Genes Dev 12 (12), 1894-1906 (1998)
   PUBMED   9637690
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from AY724508.1,
            BC091291.1, FM092358.1, DY318006.1, FM104316.1, BQ194342.1 and
            CK839839.1.
            
            On or before Nov 22, 2008 this sequence version replaced
            XM_214740.4, XM_001079125.1.
            
            Sequence Note: This RefSeq record was created from transcript data
            of mixed strains because no single transcript was available for the
            full length of the gene. The extent of this transcript is supported
            by alignment data.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AY724508.1, SRR8487226.35211.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN12840111 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-644               AY724508.1         1-644
            645-2455            BC091291.1         1-1811
            2456-2686           FM092358.1         115-345
            2687-3215           DY318006.1         130-658
            3216-3537           FM104316.1         278-599
            3538-4085           BQ194342.1         39-586              c
            4086-4133           CK839839.1         1-48                c
FEATURES             Location/Qualifiers
     source          1..4133
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="Sprague-Dawley"
                     /db_xref="taxon:10116"
                     /chromosome="2"
                     /map="2q44"
     gene            1..4133
                     /gene="Hs2st1"
                     /note="heparan sulfate 2-O-sulfotransferase 1"
                     /db_xref="GeneID:292155"
                     /db_xref="RGD:1305366"
     exon            1..402
                     /gene="Hs2st1"
                     /inference="alignment:Splign:2.1.0"
     CDS             279..1349
                     /gene="Hs2st1"
                     /EC_number="2.8.2.-"
                     /codon_start=1
                     /product="heparan sulfate 2-O-sulfotransferase 1"
                     /protein_id="NP_001093988.1"
                     /db_xref="GeneID:292155"
                     /db_xref="RGD:1305366"
                     /translation="MGLLRIMMPPKLQLLAVVAFAVAMLFLENQIQKLEESRAKLERA
                     IARHEVREIEQRHTMDGPRQDATLDEEEDIVIIYNRVPKTASTSFTNIAYDLCAKNRY
                     HVLHINTTKNNPVMSLQDQVRFVKNITSWNEMKPGFYHGHISYLDFAKFGVKKKPIYI
                     NVVRDPIERLVSYYYFLRFGDDYRPGLRRRKQGDKKTFDECVAEGGSDCAPEKLWLQI
                     PFFCGHSSECWNVGSRWAMDQAKYNLVNEYFLVGVTEELEDFIMLLEAALPRFFRGAT
                     DLYRTGKKSHLRKTTEKKLPTKQTIAKLQQSDIWKMENEFYEFALEQFQFMRAHAVRE
                     KDGDLYILAQNFFYEKIYPKSN"
     exon            403..641
                     /gene="Hs2st1"
                     /inference="alignment:Splign:2.1.0"
     exon            642..727
                     /gene="Hs2st1"
                     /inference="alignment:Splign:2.1.0"
     exon            728..866
                     /gene="Hs2st1"
                     /inference="alignment:Splign:2.1.0"
     exon            867..964
                     /gene="Hs2st1"
                     /inference="alignment:Splign:2.1.0"
     exon            965..1122
                     /gene="Hs2st1"
                     /inference="alignment:Splign:2.1.0"
     exon            1123..4116
                     /gene="Hs2st1"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 ggaagagagg gaagagggcg tgcggccgcg gggtcccgaa gccaggcggc agcggcagga
       61 gggcgccagg gccgtcgagt gggccgggga gaagcgtgca ccgggcggcc ggcgctggct
      121 cccgcgggcg ggagaccgga gaggaggagg agggggtcgc tgcggcgcgg cagcccgcgc
      181 agctccgggg tcggggtctc ctcccggcgc ttccgtcgcc ctcggggccc ccgctccccg
      241 ccccccgcgg tatgtcttga tcgcgagcgg cgggtttcat ggggctcctc aggattatga
      301 tgccgcccaa gttgcagctg ctggcggtgg tggccttcgc ggtggcgatg ctcttcttgg
      361 agaaccagat ccagaagctg gaggagtccc gggcgaagct agagagggca attgcaagac
      421 atgaagtccg agagattgaa cagcgccaca cgatggacgg ccctcggcag gatgccactc
      481 tggatgaaga agaagacatc gtcatcattt ataacagagt gcccaaaact gcaagcacct
      541 cgttcaccaa tatcgcctat gacctgtgtg caaagaatag ataccacgtt cttcacatca
      601 acactaccaa aaacaaccca gtaatgtcat tgcaggatca ggtgcgcttt gtaaagaata
      661 taaccagctg gaacgagatg aaaccaggct tctatcacgg acacatttct tacttggatt
      721 ttgcaaaatt cggggtgaag aagaagccga tttacatcaa tgtcgtcagg gacccgattg
      781 agaggctagt ttcctactat tactttctga ggtttggaga tgattacaga ccgggactaa
      841 ggaggcggaa acaaggagac aagaagacct tcgatgaatg cgtggctgag ggcggctcag
      901 actgtgctcc agagaagctc tggcttcaga tcccgttctt ctgtggccac agctcagaat
      961 gctggaatgt tggaagcaga tgggcgatgg atcaagctaa gtacaaccta gttaatgagt
     1021 acttcctggt gggggttact gaagagttgg aagacttcat catgctcctg gaggcggctc
     1081 tgccccggtt cttcaggggc gcgacggacc tctatcgtac aggaaagaaa tctcacctta
     1141 ggaaaactac agagaagaaa ctgcccacta aacagaccat cgcgaagcta cagcagtccg
     1201 acatttggaa aatggagaat gagttctatg agtttgccct ggagcagttc cagttcatga
     1261 gagcccacgc ggtccgtgag aaagatggag acctctacat cctggcccag aactttttct
     1321 atgaaaagat ttaccctaag tcgaactgag cgcaggtgtg gccagagcac tcttgatctg
     1381 gaacttgact ctgtgaccac cttctcagcc ccactgcgcg gccgatgcgt gtgtgagctg
     1441 cccgcgaagc taagagatgt cttagatgtc ttgatgtcaa gagaggagat gctggcggcc
     1501 gtcagaggtc aaggagagtt cggctctcag gcagttcact ctctcgtttt gttcccattt
     1561 tcttgatagt tacactctcc tcccggggca gggaggggcc tcgaattgcc tacaacatac
     1621 ccccggggca gggaggggcc tcgcattgcc tataacacac ccgtggaggt tagtgggaag
     1681 gcgagtacca ttccagaaga ggttctgctt ctcttgcttt tgatgaggct ttcctcacct
     1741 ctccttgaat gaagatgaat ccgtttgcca cttacccttc tggagtttgt gtctccacct
     1801 ctactgaata gtgctaataa ccgtttggca gtgtctgatt tatttttatg aatcatgtct
     1861 cacaaagttt attagaatgt ttgatcacgt tttctcagaa catttctgct gcagttgggt
     1921 ttgcccatat ttatgtaggc tctattttat tttatgatca ttagtattaa agagatcaac
     1981 tgaaaaccat gaataatgct gtaaaaaggt agaaagagtt aaagaagcag tattcctgac
     2041 tcctgccacc ccggtatcta atattgggtt aatatttcta agagtgtgga tgttatctga
     2101 ggctttttta aaggatttcc acatatcaat ataaaaaaaa aaagtgagtt taatacctgt
     2161 ctctccatgg cttatctgta accctgtaca ctgacgtctc ggtgattcca catggcgact
     2221 ccatcagtga gctgtgatgt gggtaggatt tgcctacttc tgtactttta cctgtagact
     2281 atttttacta cggtgcttta taatgtgttt taaagcattg catttacaaa agaaaaaagg
     2341 aaaatgctgt aaatattgca tattttatgt atttggacca aaaagttaca agtaagtaga
     2401 caaaagtggt tttgcaacaa ttttactatg tcgagtaaag tcatcagacc tgctattctt
     2461 gtatttctca tttagcgtta ctgttatggt gtcactaaga tgagctgacg tagcctctca
     2521 attttgatgg agaacgttgc tcgcccctga ggcaatagtg atcttgggaa gagtactgga
     2581 gtctgagctt acctctggtg ccggctctgg accttgcagc tgtgtgatgt tagtcagctc
     2641 acctgacctc tgtctgcttc agcttcctca tctcgagatc aggatacctg ctgtacctac
     2701 ctcacagggc tgttgtgagg actaaggaag acggcatggc agagagcact gtgaaagtgt
     2761 ggcgtggtgt tcaaatgtga ggtgttatca cagacgtatc tctagcctat ggttccatag
     2821 ccttcattct ttttaacaac aaaaagggaa aatccacttt taagctttat gaggaaaaag
     2881 aagacccttt ctcttgatat aaatttgtgt ttgataaata tcctctcagt gttttatctt
     2941 ccgtgcttca gcaactgttg aaatgtgaac cccctgtgag ctctcgagac tcctccttag
     3001 cagatgagga agagcgggaa agccttctgc aaacctgaaa gattgttctt gtttttatct
     3061 gactctgtca tccaagaatg gtggctatcc tacagctgtg cttgtgaaag tgtattttgt
     3121 agagtttcat ttctctatta atgcttcacc ttccccctct tcacatccag aattctgatt
     3181 cctctcagac acacttttct aagggagtaa ttatttgtat gtttaaatat ttctaagcag
     3241 gacctcatca aacaaagcat tttcaacaga aagctttaaa gtatgtattg tgtagtaagg
     3301 ggatggagag caccgtatat caggacgcac ttacagcgat taaaggaaag actgagccac
     3361 cagccccgtc aagacagaag tattaagagg acatagcaac atcctcactc tccctccgtc
     3421 ccaatgaaag tttccaggtt cctgctaaaa acaagttgtt ttcttgccca tcgctcaaga
     3481 gccttgtcaa ggcaggcctc tggctgttga ataaatctga ggttattctt gagagttcgc
     3541 aactaaactc ttaggtagtc agggcagcac ttagaacacc gactgggact ttgtgtaaat
     3601 tgttctcgtt aaagaccaga gtgcataagt cagggctctg tgcagccact caactcttag
     3661 tggtgcaaag aagctgtcac agataaagta gaagcaaagg accatggtta tagcttagtg
     3721 agacctttat ttatgaaaca gatgacttgc cagatgcaga ccatgagctg caagccctca
     3781 cactgaggga tcgtcacgga gaagccatgt ctgtgtctga accaagttcc cccaagagtt
     3841 tctctacctc ccaagtctcc cagattacaa ccccaacagc tgctttctga cccagtctct
     3901 gccgaggttt cttccttcta tcttacgtac actgctttca ggcaactcgt tttcctagct
     3961 catgagtgca aaattgggac atatttattt cttttaacgc tttttggagt gaatggattc
     4021 aaagtgtcct ttctggagat gaagaaaagg gtaggttttc ctctgtaaaa gtccttagag
     4081 catataaatt tataatcaaa taaatagaac cactgaaaaa aaaaaaaaaa aaa
//
