LOCUS       NM_001276472            3169 bp    mRNA    linear   ROD 17-FEB-2024
DEFINITION  Rattus norvegicus 3-hydroxy-3-methylglutaryl-CoA lyase like 1
            (Hmgcll1), mRNA.
ACCESSION   NM_001276472 XM_001058331 XM_345953
VERSION     NM_001276472.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 3169)
  AUTHORS   Arnedo,M., Menao,S., Puisac,B., Teresa-Rodrigo,M.E.,
            Gil-Rodriguez,M.C., Lopez-Vinas,E., Gomez-Puertas,P., Casals,N.,
            Casale,C.H., Hegardt,F.G. and Pie,J.
  TITLE     Characterization of a novel HMG-CoA lyase enzyme with a dual
            location in endoplasmic reticulum and cytosol
  JOURNAL   J Lipid Res 53 (10), 2046-2056 (2012)
   PUBMED   22847177
REFERENCE   2  (bases 1 to 3169)
  AUTHORS   Montgomery,C., Pei,Z., Watkins,P.A. and Miziorko,H.M.
  TITLE     Identification and characterization of an extramitochondrial human
            3-hydroxy-3-methylglutaryl-CoA lyase
  JOURNAL   J Biol Chem 287 (40), 33227-33236 (2012)
   PUBMED   22865860
  REMARK    GeneRIF: To evaluate the physiological role of HMGCLL1, recombinant
            protein was expressed in Pichia pastoris and demonstrated to cleave
            HMG-CoA to acetoacetate and acetyl-CoA with catalytic and affinity
            properties comparable with human mitochondrial HMGCL. The available
            results for COS1 cell expression, as well as endogenous expression
            in U87 cells, indicate that HMGCLL1 is an extramitochondrial
            hydroxymethylglutaryl-CoA lyase.
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JACYVU010000199.1.
            
            [WARNING] On Mar 26, 2024 this sequence was replaced by
            NM_001276472.3.
            
            On Feb 10, 2021 this sequence version replaced NM_001276472.1.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: FQ211656.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMD00132270, SAMD00132274
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-215               JACYVU010000199.1  9372313-9372527
            216-296             JACYVU010000199.1  9410706-9410786
            297-404             JACYVU010000199.1  9410983-9411090
            405-500             JACYVU010000199.1  9427764-9427859
            501-649             JACYVU010000199.1  9429821-9429969
            650-713             JACYVU010000199.1  9435869-9435932
            714-902             JACYVU010000199.1  9439104-9439292
            903-1028            JACYVU010000199.1  9495499-9495624
            1029-3169           JACYVU010000199.1  9499499-9501639
FEATURES             Location/Qualifiers
     source          1..3169
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="8"
                     /map="8q24"
     gene            1..3169
                     /gene="Hmgcll1"
                     /gene_synonym="RGD1565090"
                     /note="3-hydroxy-3-methylglutaryl-CoA lyase like 1"
                     /db_xref="GeneID:367112"
                     /db_xref="RGD:1565090"
     exon            1..215
                     /gene="Hmgcll1"
                     /gene_synonym="RGD1565090"
                     /inference="alignment:Splign:2.1.0"
     CDS             108..1139
                     /gene="Hmgcll1"
                     /gene_synonym="RGD1565090"
                     /EC_number="4.1.3.4"
                     /note="3-hydroxymethyl-3-methylglutaryl-Coenzyme A
                     lyase-like 1; 3-hydroxymethyl-3-methylglutaryl-CoA
                     lyase-like protein 1; 3-hydroxymethyl-3-methylglutaryl-CoA
                     lyase, cytoplasmic; 3-hydroxy-3-methylglutaryl-CoA
                     lyase-like protein 1; 3-hydroxymethyl-3-methylglutaryl-CoA
                     lyase L1; 3-hydroxymethyl-3-methylglutaryl-CoA lyase-like
                     1"
                     /codon_start=1
                     /product="3-hydroxy-3-methylglutaryl-CoA lyase,
                     cytoplasmic"
                     /protein_id="NP_001263401.1"
                     /db_xref="GeneID:367112"
                     /db_xref="RGD:1565090"
                     /translation="MGNLPSAAKHCLNYQQLLREHLWSGESVAGALDPAQEASQLSGL
                     PEYVKIVEVGPRDGLQNEKVIVPTDIKIEFINQLSQTGLSVIEVTSFVSSRWVPQMAD
                     HAEVMGGIHQYPGVRYPVLVPNLQGLQHAVAAGATEIAVFGAASESFSKKNINCSIEE
                     SMGRFEQVISSARHMNIPVRGYVSCALGCPYEGSIMPQKVTEVSKRLYSMGCYEISLG
                     DTVGVGTPGSMKTMLESVMKEIPPGALAVHCHDTYGQALANILTALQMGINVVDSAVS
                     GLGGCPYAKGASGNVATEDLIYMLNGMGLNTGVDLHKVMEAGDFICKAVNKTTNSKVA
                     QASFKARLE"
     exon            216..296
                     /gene="Hmgcll1"
                     /gene_synonym="RGD1565090"
                     /inference="alignment:Splign:2.1.0"
     exon            297..404
                     /gene="Hmgcll1"
                     /gene_synonym="RGD1565090"
                     /inference="alignment:Splign:2.1.0"
     exon            405..500
                     /gene="Hmgcll1"
                     /gene_synonym="RGD1565090"
                     /inference="alignment:Splign:2.1.0"
     exon            501..649
                     /gene="Hmgcll1"
                     /gene_synonym="RGD1565090"
                     /inference="alignment:Splign:2.1.0"
     exon            650..713
                     /gene="Hmgcll1"
                     /gene_synonym="RGD1565090"
                     /inference="alignment:Splign:2.1.0"
     exon            714..902
                     /gene="Hmgcll1"
                     /gene_synonym="RGD1565090"
                     /inference="alignment:Splign:2.1.0"
     exon            903..1028
                     /gene="Hmgcll1"
                     /gene_synonym="RGD1565090"
                     /inference="alignment:Splign:2.1.0"
     exon            1029..3169
                     /gene="Hmgcll1"
                     /gene_synonym="RGD1565090"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 agctcggacc ccgggcaccc gcgggacgcg ctcccggctt ccaccccgct cccggctcgg
       61 ctcgggccgc gggatccagc accgcccgcg gcaccaaggc tgctgccatg gggaatctac
      121 catctgctgc gaagcactgc ctcaactacc agcagcttct ccgcgagcat ctctggagcg
      181 gagaatcagt ggcaggggcg ctggacccgg cgcaggaagc atcccagtta tctggactcc
      241 ctgaatatgt gaaaatagta gaagttggac ctagggatgg attgcagaac gaaaaggtta
      301 tagttcctac agatataaaa attgaattca tcaatcaact ttcccaaact ggcttatctg
      361 taatagaagt aaccagtttt gtgtcttcca gatgggtacc acagatggct gaccatgctg
      421 aagtgatggg aggcattcat cagtatccag gagttcgcta tcctgtcctt gtacctaatc
      481 ttcagggttt gcagcatgct gttgccgctg gggccacgga gatagcagtg tttggtgctg
      541 cttcagaatc tttcagcaag aagaacatta actgctccat cgaagaaagc atgggacggt
      601 ttgagcaagt catcagctct gcaagacaca tgaacatccc agtgcgaggg tatgtgtcct
      661 gcgccctggg ctgtccctat gaaggaagca tcatgccaca gaaagtcaca gaagtttcca
      721 agagattgta cagcatgggc tgctatgaga tctctttggg agacacagtt ggggttggta
      781 ctccaggcag catgaagaca atgttggaaa gtgtcatgaa agagatccct ccgggggctc
      841 ttgctgttca ctgccatgac acgtatggac aagccctcgc aaacatccta acggctcttc
      901 agatgggaat taacgtggtg gactcggctg tatctggatt aggtggctgc ccttatgcaa
      961 aaggtgcttc tgggaatgta gccactgagg acttgatata tatgcttaat ggcatggggc
     1021 tcaatacagg tgtggatctc cacaaagtga tggaagctgg ggactttatt tgcaaggcag
     1081 tgaataaaac cacaaactct aaggttgccc aagcctcctt caaagcacga cttgaatgag
     1141 tttatggttt gagaaagaga agagccattc ccgccccagt tcatcacagc atcatcagtg
     1201 ccaacgcatt ctgaagtgga agtaaataag aaagagctgg gaaaggagat atgctttgga
     1261 aagtttatca gtgggtagac catgaattcg gtggaaggca agacgaggca tttgttcaac
     1321 tgcaagctgg gaattaacac cggtgcacgt ccttctgggg gtggacaagg cctgatcatt
     1381 tgctcttaca gaaatcatta tattttttag cttagtagag ctgaaaattg acttcagatt
     1441 tctctaagta tcaagtgctg tgttaatgcc tcctcaagcc accttaccgc cgggtacaca
     1501 ttgttagtgg tttatgagcc tcgacacaat gcagagtgtg tggccctgaa gttgtgtgtt
     1561 tttcctctgg atcccggttc tccatttgcc aagtttgtta agttatggac caagagccaa
     1621 agtttcatct gacccaacat aatttttagc aatagaactt ttatatttca agtgcggcta
     1681 acatctgtta actatttcaa tgactttatt gtgctccaag ttgctgtggt caatggcaag
     1741 acgccacatc ctgaaagcac gccgtacctc ccatgtttgt tccatgcgtt cagtttgctg
     1801 tgttttacat ttaatcagtt gtagaaaaaa tagtccacaa tgttatttaa atatggagaa
     1861 actatatggt gtgattttta ttctggtgtc tgagggacag taatgacagg agactgacgt
     1921 agacactctt gtctatttcc ataatatcta ggttactttt gaattgccca aggaaatact
     1981 attgtgattt taagaatttt aatataattt taactgatga aaagtctgtt ttccttgact
     2041 taatattctc tctatctgtt tgtttttatt tgactttaga atatttattt tgaagtggaa
     2101 tgacagaaat attatctttt gtgccagaaa gcattgtaag ggataatgaa aattaataca
     2161 ttaaagatca aggcctgggg ctatggctca gtggtcaaga gcactggcca tccttctaga
     2221 gggcatagac ttgattccaa gcgcccatat gatgattcaa tagcatttat aactccagtt
     2281 tcagggaatc taatggcttc ttctggcctc ttcttgacta gtcaggcaag aaggacacag
     2341 gcatatgtgc aggtaaatca tccattaatg caaaatagcg tagacatcct agaaaatagt
     2401 aaaaatattg aaatgaaaat attcaagaca ataaggatat tgattagaag tacaatatcg
     2461 ttagaggtat atggtaggga aaactggaat ggaaacatac agcttgacaa agaaacctaa
     2521 atttccatag aagactccga cttgtcagct tgtaacggtt cttcaggagg gtctgttacc
     2581 gcatgctttc ctcaagcgca ctgaaagcag ttctgtgctt gggggctcag gttggaacat
     2641 gaatggcaca gaggcagtaa agtggcattt gattgtggag ctgccttagt accccctccc
     2701 tgatcatctc ttttgactag ggtgctgcca ggcatcgaca tcaaaggttc ttttttctcc
     2761 attttctgag gaaattgatt ttccctatca gagatgtgga gtgttcagtt ttgagatatt
     2821 tgggaaaaca tctgtttatg cagactactt gtaaaatcag tgaccagcat tttcgtttgg
     2881 aaatgaaact caaagttctt tcacagacgg agcacataga aacctcagat atagtgctgt
     2941 acagttgcat ggtgcctacc tccgcagatc agctaatttt catgactacc ttgatactgt
     3001 ctacttacac ttgacatagc tagtttatgt tttcttctct ctctctctct ctctctctct
     3061 ctctctctct ctctctctct ctctctctct gctgtcttaa tttcttagga agaattttct
     3121 caactgccca ggaaaccttg cagtcactgc aggacccatt ggaaaaaaa
//
