LOCUS       NM_024386               1391 bp    mRNA    linear   ROD 01-APR-2024
DEFINITION  Rattus norvegicus 3-hydroxy-3-methylglutaryl-CoA lyase (Hmgcl),
            mRNA; nuclear gene for mitochondrial product.
ACCESSION   NM_024386
VERSION     NM_024386.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 1391)
  AUTHORS   Martin,J., Balmer,M.L., Rajendran,S., Maurhofer,O., Dufour,J.F. and
            St-Pierre,M.V.
  TITLE     Nutritional stress exacerbates hepatic steatosis induced by
            deletion of the histidine nucleotide-binding (Hint2) mitochondrial
            protein
  JOURNAL   Am J Physiol Gastrointest Liver Physiol 310 (7), G497-G509 (2016)
   PUBMED   26767982
REFERENCE   2  (bases 1 to 1391)
  AUTHORS   Arnedo,M., Menao,S., Puisac,B., Teresa-Rodrigo,M.E.,
            Gil-Rodriguez,M.C., Lopez-Vinas,E., Gomez-Puertas,P., Casals,N.,
            Casale,C.H., Hegardt,F.G. and Pie,J.
  TITLE     Characterization of a novel HMG-CoA lyase enzyme with a dual
            location in endoplasmic reticulum and cytosol
  JOURNAL   J Lipid Res 53 (10), 2046-2056 (2012)
   PUBMED   22847177
REFERENCE   3  (bases 1 to 1391)
  AUTHORS   Montgomery,C., Pei,Z., Watkins,P.A. and Miziorko,H.M.
  TITLE     Identification and characterization of an extramitochondrial human
            3-hydroxy-3-methylglutaryl-CoA lyase
  JOURNAL   J Biol Chem 287 (40), 33227-33236 (2012)
   PUBMED   22865860
REFERENCE   4  (bases 1 to 1391)
  AUTHORS   Ghosh,D. and Berg,J.M.
  TITLE     A proteome-wide perspective on peroxisome targeting signal
            1(PTS1)-Pex5p affinities
  JOURNAL   J Am Chem Soc 132 (11), 3973-3979 (2010)
   PUBMED   20178365
REFERENCE   5  (bases 1 to 1391)
  AUTHORS   Leipnitz,G., Seminotti,B., Fernandes,C.G., Amaral,A.U.,
            Beskow,A.P., da Silva Lde,B., Zanatta,A., Ribeiro,C.A., Vargas,C.R.
            and Wajner,M.
  TITLE     Striatum is more vulnerable to oxidative damage induced by the
            metabolites accumulating in 3-hydroxy-3-methylglutaryl-CoA lyase
            deficiency as compared to liver
  JOURNAL   Int J Dev Neurosci 27 (4), 351-356 (2009)
   PUBMED   19460629
  REMARK    GeneRIF: Results describe the in vitro effects of
            3-hydroxy-3-methylglutarate metabolites in
            3-hydroxy-3-methylglutaryl-CoA lyase deficiency on parameters of
            oxidative stress in striatum and liver.
REFERENCE   6  (bases 1 to 1391)
  AUTHORS   Roberts,J.R., Narasimhan,C., Hruz,P.W., Mitchell,G.A. and
            Miziorko,H.M.
  TITLE     3-Hydroxy-3-methylglutaryl-CoA lyase: expression and isolation of
            the recombinant human enzyme and investigation of a mechanism for
            regulation of enzyme activity
  JOURNAL   J Biol Chem 269 (27), 17841-17846 (1994)
   PUBMED   8027038
REFERENCE   7  (bases 1 to 1391)
  AUTHORS   Auestad,N., Korsak,R.A., Morrow,J.W. and Edmond,J.
  TITLE     Fatty acid oxidation and ketogenesis by astrocytes in primary
            culture
  JOURNAL   J Neurochem 56 (4), 1376-1386 (1991)
   PUBMED   2002348
REFERENCE   8  (bases 1 to 1391)
  AUTHORS   Curi,R., Williams,J.F. and Newsholme,E.A.
  TITLE     Formation of ketone bodies by resting lymphocytes
  JOURNAL   Int J Biochem 21 (10), 1133-1136 (1989)
   PUBMED   2573547
REFERENCE   9  (bases 1 to 1391)
  AUTHORS   Shah,J. and Bailey,E.
  TITLE     Changes in the activities of the enzymes of hepatic ketogenesis in
            the rat between late fetal life and weaning
  JOURNAL   Enzyme 22 (1), 35-40 (1977)
   PUBMED   13990
REFERENCE   10 (bases 1 to 1391)
  AUTHORS   Williamson,D.H., Bates,M.W. and Krebs,H.A.
  TITLE     Activity and intracellular distribution of enzymes of ketone-body
            metabolism in rat liver
  JOURNAL   Biochem J 108 (3), 353-361 (1968)
   PUBMED   5667251
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from
            JAXUCZ010000005.1.
            
            On Nov 26, 2020 this sequence version replaced NM_024386.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: Y10054.1, BC061797.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760383, SAMEA5760386
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: inferred from homology
            RefSeq Select criteria             :: based on conservation,
                                                  expression, longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-76                JAXUCZ010000005.1  153461808-153461883
            77-160              JAXUCZ010000005.1  153465508-153465591
            161-268             JAXUCZ010000005.1  153467373-153467480
            269-364             JAXUCZ010000005.1  153468102-153468197
            365-513             JAXUCZ010000005.1  153470031-153470179
            514-577             JAXUCZ010000005.1  153471948-153472011
            578-766             JAXUCZ010000005.1  153473042-153473230
            767-892             JAXUCZ010000005.1  153473803-153473928
            893-1391            JAXUCZ010000005.1  153475051-153475549
FEATURES             Location/Qualifiers
     source          1..1391
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="5"
                     /map="5q36"
     gene            1..1391
                     /gene="Hmgcl"
                     /note="3-hydroxy-3-methylglutaryl-CoA lyase"
                     /db_xref="GeneID:79238"
                     /db_xref="RGD:620554"
     exon            1..76
                     /gene="Hmgcl"
                     /inference="alignment:Splign:2.1.0"
     CDS             17..994
                     /gene="Hmgcl"
                     /EC_number="4.1.3.4"
                     /note="hydroxymethylglutaryl-CoA lyase, mitochondrial; HL;
                     HMG-CoA lyase; 3-hydroxy-3-methylglutarate-CoA lyase;
                     3-hydroxy-3-methylglutaryl-Coenzyme A lyase;
                     3-hydroxymethyl-3-methylglutaryl-Coenzyme A lyase;
                     3-hydroxymethyl-3-methylglutaryl-CoA lyase"
                     /codon_start=1
                     /product="hydroxymethylglutaryl-CoA lyase, mitochondrial
                     precursor"
                     /protein_id="NP_077362.1"
                     /db_xref="GeneID:79238"
                     /db_xref="RGD:620554"
                     /translation="MATVRKAFPQRLVGLASLRAASTSSMGTLPKRVKIVEVGPRDGL
                     QNEKSIVPTPVKIKLIDMLSEAGLPVIEATSFVSPKWVPQMADHSDVLKGIQKFPGIN
                     YPVLTPNMKGFEEAVAAGAKEVSIFGAASELFTRKNVNCSIEESFQRFDGVMQAARAA
                     SISVRGYVSCALGCPYEGKVSPAKVAEVAKKLYSMGCYEISLGDTIGVGTPGLMKDML
                     TAVLHEVPVAALAVHCHDTYGQALANTLVALQMGVSVVDSSVAGLGGCPYAKGASGNL
                     ATEDLVYMLTGLGIHTGVNLQKLLEAGDFICQALNRKTSSKVAQATCKL"
     exon            77..160
                     /gene="Hmgcl"
                     /inference="alignment:Splign:2.1.0"
     exon            161..268
                     /gene="Hmgcl"
                     /inference="alignment:Splign:2.1.0"
     exon            269..364
                     /gene="Hmgcl"
                     /inference="alignment:Splign:2.1.0"
     exon            365..513
                     /gene="Hmgcl"
                     /inference="alignment:Splign:2.1.0"
     exon            514..577
                     /gene="Hmgcl"
                     /inference="alignment:Splign:2.1.0"
     exon            578..766
                     /gene="Hmgcl"
                     /inference="alignment:Splign:2.1.0"
     exon            767..892
                     /gene="Hmgcl"
                     /inference="alignment:Splign:2.1.0"
     exon            893..1391
                     /gene="Hmgcl"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gagactccgg gcgaagatgg cgacagtgag gaaggctttc ccacagaggc tggtgggctt
       61 ggcgtccctc cgggctgcga gcacctcctc catgggcacc ttgccgaagc gggtgaagat
      121 cgtggaagtc ggcccccgag atggtctgca gaatgaaaag agtatcgtgc cgacgccagt
      181 gaaaatcaaa ctgatagaca tgctatccga agcagggctc ccggtcatcg aggccaccag
      241 ctttgtctct cccaagtggg tgccgcagat ggctgaccac tctgacgtct tgaagggcat
      301 tcagaagttt cccggcatca actacccggt cctgacacca aacatgaaag gctttgagga
      361 agcggtagct gcaggtgcca aggaagtgag catctttggg gctgcgtccg agctcttcac
      421 ccggaagaat gtgaactgct ctatagagga gagtttccag cgctttgatg gggtcatgca
      481 ggccgcgagg gctgccagca tctctgtgag agggtatgtc tcctgtgccc tcggatgtcc
      541 ctacgagggg aaggtctccc cggctaaagt tgctgaggtc gccaagaagt tgtactcaat
      601 gggctgctat gagatctccc ttggggacac cattggcgta ggcacgccag gactcatgaa
      661 agacatgctg actgctgtcc tgcatgaagt gcctgtggcc gcattggctg tccactgcca
      721 tgacacctat ggccaagctc tggccaacac gttggtggcc ctgcagatgg gagtgagcgt
      781 tgtggactcc tcggtggcag gactcggagg ctgtccctat gcaaaggggg cgtcaggaaa
      841 cttggctacc gaggacctgg tctacatgct gactggctta gggattcaca cgggtgtgaa
      901 cctccagaag ctcctagaag ccggggactt catctgtcaa gccctgaaca gaaaaaccag
      961 ttccaaagtg gcacaggcca cctgcaaact ctgagcccct tgttcaccta aaccggaact
     1021 gtgggagttg ggtgtacaca atgattcctg gatggggaaa tggaatgaag gcaaatgagc
     1081 cggcctcaca gaggtccctc tcctacatag aagggctaga gctgccagca cgcccggacc
     1141 agctccccag agctgcgtgc ctaagcactg cttgggctgg ccctgggtga gtccactagc
     1201 cagcagagct gacatccatg tgccacgacc gcgggtccca tgttctacct ctgaggacag
     1261 cagcgccttt gctaaaatgg tgggctcaat ctgctgcggt ggccgactgc caactccagc
     1321 gtctctggga aatctctgta cgtgattctt gaaaacagct tatgtaatta aaggtttaat
     1381 tttctaatat c
//
