LOCUS       NM_001025047            1446 bp    mRNA    linear   ROD 13-FEB-2025
DEFINITION  Rattus norvegicus 5-hydroxymethylcytosine binding, ES cell specific
            (Hmces), mRNA.
ACCESSION   NM_001025047 XM_575599
VERSION     NM_001025047.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 1446)
  AUTHORS   Shukla,V., Halabelian,L., Balagere,S., Samaniego-Castruita,D.,
            Feldman,D.E., Arrowsmith,C.H., Rao,A. and Aravind,L.
  TITLE     HMCES Functions in the Alternative End-Joining Pathway of the DNA
            DSB Repair during Class Switch Recombination in B Cells
  JOURNAL   Mol Cell 77 (2), 384-394 (2020)
   PUBMED   31806351
  REMARK    Erratum:[Mol Cell. 2020 Mar 5;77(5):1154. doi:
            10.1016/j.molcel.2020.02.008.. PMID: 32142687]
REFERENCE   2  (bases 1 to 1446)
  AUTHORS   Halabelian,L., Ravichandran,M., Li,Y., Zeng,H., Rao,A., Aravind,L.
            and Arrowsmith,C.H.
  TITLE     Structural basis of HMCES interactions with abasic DNA and
            multivalent substrate recognition
  JOURNAL   Nat Struct Mol Biol 26 (7), 607-612 (2019)
   PUBMED   31235913
REFERENCE   3  (bases 1 to 1446)
  AUTHORS   Mohni,K.N., Wessel,S.R., Zhao,R., Wojciechowski,A.C., Luzwick,J.W.,
            Layden,H., Eichman,B.F., Thompson,P.S., Mehta,K.P.M. and Cortez,D.
  TITLE     HMCES Maintains Genome Integrity by Shielding Abasic Sites in
            Single-Strand DNA
  JOURNAL   Cell 176 (1-2), 144-153 (2019)
   PUBMED   30554877
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from
            JAXUCZ010000004.1.
            
            On Nov 27, 2020 this sequence version replaced NM_001025047.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC083690.1, SRR22399481.1318578.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760383, SAMEA5760386
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-205               JAXUCZ010000004.1  121950840-121951044
            206-349             JAXUCZ010000004.1  121954082-121954225
            350-475             JAXUCZ010000004.1  121958121-121958246
            476-654             JAXUCZ010000004.1  121962337-121962515
            655-847             JAXUCZ010000004.1  121969473-121969665
            848-1446            JAXUCZ010000004.1  121972339-121972937
FEATURES             Location/Qualifiers
     source          1..1446
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="4"
                     /map="4q34"
     gene            1..1446
                     /gene="Hmces"
                     /note="5-hydroxymethylcytosine binding, ES cell specific"
                     /db_xref="GeneID:500251"
                     /db_xref="RGD:1559800"
     exon            1..205
                     /gene="Hmces"
                     /inference="alignment:Splign:2.1.0"
     CDS             23..1084
                     /gene="Hmces"
                     /note="UPF0361 protein C3orf37 homolog; putative peptidase
                     SRAPD1; SRAP domain-containing protein 1; ES cell-specific
                     5hmC-binding protein; embryonic stem cell-specific
                     5-hydroxymethylcytosine-binding protein;
                     5-hydroxymethylcytosine (hmC) binding, ES cell-specific;
                     peptidase HMCES; putative endonuclease HMCES"
                     /codon_start=1
                     /product="abasic site processing protein HMCES"
                     /protein_id="NP_001020218.1"
                     /db_xref="GeneID:500251"
                     /db_xref="RGD:1559800"
                     /translation="MCGRTSCHLPRDALTRACAYLDRQGRRQLPQWRDPDKYCPSYNK
                     SPQSSSPVLLSRLHFEKDADSSDRIIFPMRWGLVPSWFKESDPSKLQFNTSNCRSDTI
                     MEKQSFKAPLGKGRRCVVLADGFYEWQRCQGTNQRQPYFIYFPQSKTEKSGENSGSDS
                     LNNKEEVWDNWRLLTMAGIFDCWEPPKGERLYSYSIITVDSCRGLSDIHSRMPAILDG
                     EEAVSKWLDFGEVSTQEALKLIHPIDNITFHPVSPVVNNSRNNTPECLAPADLLVKKE
                     PKASGSSQRMMQWLATKSPKKEVPDSPKKDASGLPQWSSQFLQKSPLPTKRGASSSLL
                     DRWLKQEKEDEPVAKRPNS"
     exon            206..349
                     /gene="Hmces"
                     /inference="alignment:Splign:2.1.0"
     exon            350..475
                     /gene="Hmces"
                     /inference="alignment:Splign:2.1.0"
     exon            476..654
                     /gene="Hmces"
                     /inference="alignment:Splign:2.1.0"
     exon            655..847
                     /gene="Hmces"
                     /inference="alignment:Splign:2.1.0"
     exon            848..1446
                     /gene="Hmces"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gtctgagagg cattgctgaa gtatgtgcgg gcgaacgtcc tgtcacttgc ctagagatgc
       61 tctcactaga gcctgtgcct atctggatcg gcagggcagg cgtcagctcc cgcagtggag
      121 ggaccctgat aagtactgcc cctcctacaa caagagcccg cagtctagca gcccagtgct
      181 tctctccaga ctgcactttg aaaaggatgc agattcatca gatcggataa tttttcccat
      241 gcgatggggc ttagtcccat cttggttcaa agaaagtgat ccttccaagc tgcagttcaa
      301 cacttccaac tgccgtagtg ataccataat ggagaagcag tcgttcaagg ctcctctggg
      361 gaaaggacgg cggtgtgttg tcttagcaga tggattctac gagtggcagc gatgtcaggg
      421 aacaaaccag aggcagccgt acttcatcta ttttcctcaa agcaaaacag agaagtcagg
      481 tgagaacagt ggttcagaca gccttaacaa caaggaagag gtctgggaca actggaggct
      541 gctgacaatg gcagggatct ttgactgttg ggaaccccca aagggagagc gcctgtattc
      601 ctacagcatc atcactgtgg attcctgcag aggtctgagt gacatccaca gcaggatgcc
      661 tgccatactg gatggagaag aggcggtctc caaatggctt gactttggtg aggtctctac
      721 tcaggaagct ctgaagctaa tccaccccat agataacatc accttccatc cagtttctcc
      781 agtggtgaac aattcccgaa acaacactcc tgagtgtctg gctcctgctg acttgctggt
      841 taagaaggag cccaaggcaa gtggcagtag tcaaaggatg atgcaatggc tggctacaaa
      901 gtcacccaaa aaggaagtcc ctgactcacc caaaaaggat gcatcaggtc taccccagtg
      961 gtccagccag tttctacaga agagcccatt gccgactaaa agaggtgcca gcagcagcct
     1021 cctggatcga tggctgaagc aggagaagga ggatgagcct gtggccaaga ggccaaacag
     1081 ctagtgacag agtcctccat accaggcctc agcctgatac agtattactg atgccaccag
     1141 ctgcccggaa ttgtatccat ttacttggga aggtggtgat tctgtcagct gacctttatg
     1201 gacctagaag aacaagtcta ctgtgagaca gcctaagagg gctagctacc atggctccct
     1261 atgggccctg ctatccctgg ctccttgcta gaagctttcc tgtccctcct gttccacaaa
     1321 ggaggctctt caaaatctct gggctctcct ttagagttaa gctttttaaa tgtatttgtt
     1381 ctaaataaat tgcttttgat aaaccttggc tggcaagagt tctggctctt gcccagtcct
     1441 ccctga
//
