LOCUS       XM_039088798            5404 bp    mRNA    linear   ROD 11-JUN-2023
DEFINITION  PREDICTED: Rattus norvegicus holocarboxylase synthetase (Hlcs),
            transcript variant X8, mRNA.
ACCESSION   XM_039088798
VERSION     XM_039088798.1
DBLINK      BioProject: PRJNA677964
KEYWORDS    RefSeq.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_051346.1) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            [WARNING] On Feb 22, 2024 this sequence was replaced by
            XM_039088798.2.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Updated annotation
            Annotation Name             :: GCF_015227675.2-RS_2023_06
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.1
            Annotation Method           :: Best-placed RefSeq; Gnomon;
                                           cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 06/06/2023
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5404
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN/NHsdMcwi"
                     /db_xref="taxon:10116"
                     /chromosome="11"
                     /sex="male"
                     /tissue_type="kidney"
                     /geo_loc_name="USA: Wisconsin, Milwaukee, Medical College
                     of Wisconsin"
                     /collection_date="2019-03-08"
                     /collected_by="Rebecca Schilling"
     gene            1..5404
                     /gene="Hlcs"
                     /note="holocarboxylase synthetase; Derived by automated
                     computational analysis using gene prediction method:
                     Gnomon. Supporting evidence includes similarity to: 27
                     ESTs, 2 long SRA reads, 3 Proteins, and 100% coverage of
                     the annotated genomic feature by RNAseq alignments,
                     including 70 samples with support for all annotated
                     introns"
                     /db_xref="GeneID:288240"
                     /db_xref="RGD:1565360"
     CDS             450..2621
                     /gene="Hlcs"
                     /codon_start=1
                     /product="biotin--protein ligase isoform X1"
                     /protein_id="XP_038944726.1"
                     /db_xref="GeneID:288240"
                     /db_xref="RGD:1565360"
                     /translation="MEDRLQMDNGLIAQKIVSVHLKDPALKELSKASNKQVQAPLPSP
                     EASLEAQPAQGVMEQAGQGDWKAAGEGPSPQRRGCAPVSESAADGDPGQGSTELCQLH
                     LSSCHECLELENSTIESVRCASAENIPDLPHDCSSSVEGTAGELCPERKGKRVNISGK
                     APNILLYVGSGSEEALGQLQQVRSVLTDCVDTDSYTLYHLLEDSALRDPWPDNCLLLV
                     IASRDPIPKGIHHRFMAYLSQGGKVLGLSSSFTFGDLRVARRDVLRNTAQNLVFSKAD
                     GSEVRLSVLSSGYVYEEGPSLGRLQGHLENEDKDKMIVHVPFGTHGGEAILCQVHLEL
                     PPSAPLVQTTDDFNVLKSSNTRRLEVLKEILTALGLSCDAPQAPALTPLYLLLAAEEI
                     QGPFMQWLARHVDPEGVIKSSKLSLKFVSSYTSEAEITPSSIPVVTDPEGFSSEHFNL
                     ETYRQNLQSTRLGRVILFAEVTSTTMSLLDGLMFEMPQEMGLIAIAVRQTQGKGRGPN
                     AWLSPVGCALSTLLVSIPLRSQLGQRIPFVQHLMSLAVVEAVRSIPEYEDINLRVKWP
                     NDIYYSDLMKIGGVLVNSTLVGETFYILIGCGFNVTNSNPTICINDLIEEHNKQNKAG
                     LKPLQADCLIARAVTVLEKLIDRFQDQGPDGVLPLYYKYWAHSGQQVRLGSSEGPAVS
                     IVGLDDSGFLQVHQEGGGVVSVHPDGNSFDMLRNLIVPKRP"
ORIGIN      
        1 acgaaggaca gcatccctta aggaccagcc tgctgtgggg caggctgagc tactgaggag
       61 cgaccctgtg tgtataggtc ctggaggtcc tggaggtccc cgaagtttca cgtgtggctg
      121 gaggccagag cctggctggg gtgtggccag gatctggggc tgcagggatc tggccctttc
      181 cctggtctgt tcctgctcac gaggctgcca cctgcctgtg gttggcaaag tcgcgccgcc
      241 ccattcccgt gcatttccct gcttgttacc gctcctggag ctgcgggtag ctcagaccag
      301 tagcatgggc gagggaccgt ggccgcccgg cgttacaggt tgtaaaatgg tcagactgct
      361 gtttgccgtt agcttgccgc cctggggatc cctaccaact aattgctaag gcaagcgtgg
      421 acaacttcag caagctgggg gtggccttca tggaggacag gctccagatg gataacggcc
      481 tgatagccca gaagatcgtt tcagttcact taaaggaccc tgctctgaag gaactcagca
      541 aggcctccaa caaacaagtg caggccccac tcccgagccc agaggcttcc ctggaggccc
      601 agcctgcgca aggtgtcatg gagcaagctg gtcaaggtga ctggaaggct gctggtgaag
      661 gcccttcccc acagaggaga ggctgtgccc ctgtaagtga gtctgctgca gatggagatc
      721 cagggcaagg ctccactgag ctctgtcagc ttcacctgtc cagctgccac gagtgtttgg
      781 aacttgagaa cagcaccatc gagtcggtgc ggtgtgcgtc tgcagagaac attccagatc
      841 ttccccatga ttgcagcagc agtgtggagg gtactgcggg cgaactctgc cctgaacgga
      901 aagggaagag agtcaacatc tcgggaaagg cgcccaacat cctgctgtac gtgggctctg
      961 gttccgagga agccctgggc cagctccagc aggtgcgatc tgtcctgaca gactgtgtgg
     1021 acacggacag ctacactctc taccacctgc tggaagacag tgctctgagg gacccgtggc
     1081 cagacaactg cctcctgtta gtcattgcca gcagggaccc tatccccaaa ggcatacacc
     1141 acaggttcat ggcctatctt tctcagggag ggaaggtgct gggcctgtct tcctccttca
     1201 ccttcggtga ccttcgggtg gccaggagag acgtgctacg gaacacagcc cagaacttgg
     1261 ttttctctaa ggctgatggg agtgaggtac ggctcagtgt cctgagcagc ggctacgttt
     1321 atgaggaagg cccgagcctc ggccggctcc agggccacct ggagaatgaa gacaaggata
     1381 aaatgattgt gcatgtgcct tttggaaccc acggaggtga agccattctc tgccaggtgc
     1441 atctggagct ccccccaagc gctcccctgg tgcagaccac agacgacttt aatgtgctca
     1501 aatccagcaa cacgagaaga ctcgaggtcc ttaaggagat cctgactgcg ctcggtctga
     1561 gctgtgacgc tccacaggct cctgccttaa caccactgta cttactactg gctgccgagg
     1621 aaatccaggg tccgtttatg cagtggcttg caaggcatgt ggatcctgaa ggagtcataa
     1681 agtccagcaa gctctccctt aagttcgtct cctcctacac atctgaagca gagatcaccc
     1741 cgtcttccat ccctgtggtc accgacccgg aaggcttctc atcagagcac tttaacctag
     1801 agacctatcg acagaatctg cagagcacgc gcctcgggag agtaattctg tttgcagaag
     1861 tgacctctac gaccatgagt ctcctggatg ggttgatgtt tgagatgccg caggaaatgg
     1921 gcttaatcgc cattgcagtt cggcagaccc agggcaaagg aaggggcccg aatgcctggc
     1981 tgagccccgt gggatgtgcc ctttccactc tgctagtctc catcccccta aggtcacaac
     2041 tgggacaaag aattccattt gtccagcatt tgatgtcact ggccgtggtg gaggcagtgc
     2101 ggtctatccc ggagtatgag gatatcaact tacgagtgaa gtggcccaac gatatttatt
     2161 acagcgatct catgaagatc ggcggggttc tggttaattc aacacttgtg ggagaaacat
     2221 tttatatcct cattggctgt ggattcaatg tgactaacag caatcctacc atatgtatca
     2281 atgacctcat cgaagaacac aataagcaga acaaggcagg cctgaagccc ttacaagccg
     2341 actgtctcat agccagggct gtgaccgtgc tggagaaact gattgacagg tttcaggacc
     2401 aagggcccga cggcgttctc cccctgtatt ataaatactg ggcgcacagt ggtcagcaag
     2461 tccgcctggg cagcagcgag gggccagcgg tgtctatcgt gggtcttgat gattccgggt
     2521 tcctgcaggt ccaccaagag ggtggtgggg ttgtgagcgt gcatccagat ggcaactcct
     2581 ttgacatgct gaggaacctc attgtcccca agcgaccgta aatgccgcct ccacatcccc
     2641 agacagctgg gggttgctgc tgtgcagcag tggtggcggc agcatcaggg aaactgctcc
     2701 caggatgtcc tgggaggtca gagcaggctc tgcttgctct gctgtctctg cctgcctagg
     2761 gcctgggaaa ctcatgcagt cagaccagcg ctgttgttaa ctctttaggt tgcgggttct
     2821 ccgcccccac ccccgtccca cccctgggat tgggtttttg ttttttatct ctgtggtttt
     2881 attgggtgta tgaaggggca tctggtgagg actgatggat aggtgaagaa gaatcagttt
     2941 agcttaggag tttagtttgc cttgcgtgtg tagaagagct cttccccaca tggcctggat
     3001 ctgtgtgtac tgggggagaa tggggagcaa gggggtggga atagcgatga gattatacaa
     3061 ataccccaag gagtgaaggc cgttagccgt ctgctgttaa aacgagggac cccacagctc
     3121 aggtgagtgc tgagtgctgt cccagaatgc acaggcatta acttacacct tgggttgttg
     3181 ctggttttct tagtcaggtt gggcttcttg tagtagcctg gaacacagga ctgaaggatg
     3241 gccgcagaga cgctgctagc cagtgtggtc ccctttccag ggtcagatgt gtcacgtata
     3301 gatgtttaaa ccatgatggt agcttccttg gaggcagagg cttgcccagg ctgatggaga
     3361 tatgggctca ctgtctggcc cacacaaggg agggttcttg gaacttttaa acccattagt
     3421 gacaggctct gtttgttaga agtggctctg caccccatca gcctgtgtcc tcaagcagga
     3481 gactgcagca ctgtactgta aagtcatgat tctctgcccc cgatttccta acgtcaggaa
     3541 agagacagag attgacacaa actgccatca gtttggagag atatatgtgc atgcagtgtg
     3601 tgtgtgtgtg tgtgtgtgtg tgtatgtgtg tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg
     3661 tgtttaaaag gagatgttca aaatttgatg tgctaaaagt ctccgtggaa gaacaaagga
     3721 gaggaagcct ttctaggttt gtgcttccag atgtgctcca tgcttttcca tgtacctccg
     3781 gtctctgtgg agcttgtgaa tcataccatc gctgcccagc ctaaaagaac cagttcctgc
     3841 tgcactcctg cttcctgcca cttccactga agtgggtgga aaggaaacga tggaacagaa
     3901 cacttagctt tgaacaggat cctcgtcaca aacgagtccc atctttaccc agcttctaca
     3961 gtcaggccca ccccagaaag cagagttttc agtacggaaa cccagccacc ttcagcctag
     4021 cacagacagc cccatttgaa ccaaaaatag cagggtgaac tacgttgagg gaatgcaaca
     4081 ttaaaagaac ttgaaagtaa cttctctggc aaataagact ttgattaaat cttttctcaa
     4141 aagaaaaaga taaatgtata aattttagtt ccggtatttt ttttattcat gcgagtttaa
     4201 ataacactgt tcaccaccca aaattacaaa aatgacgggc cctcgaggga gcatctgtaa
     4261 aatatcatca agaacgaaga tctccaggct tgatgttgca aagcttcgtc cctcagacct
     4321 tgtaatataa ggccgttcct ctcccagggc caccatcact acatttacat ttctatgggg
     4381 gattatttaa tgaaaaacat gttacggttt aggctgaagg cctattctgg atagttctgc
     4441 tttggggaaa aatgttatca tttaatttcc tttctgtaag ttaaaactaa tgaagtgtgg
     4501 tcctgtcaga gcagacagag atgctccggg catgccgatt gggtgttctt gcttatgcag
     4561 acctggcact cctggtcttg agagacaaaa cggcctgaag ccgatgctgt atctcccatt
     4621 agcctgactt tgcctcacat gaaccaaaga cttcaaactc catatcgtag agagcattct
     4681 gctgtcgaag gtgtgaaggc tgccactgta cctgctgtgt gcgtgtgtgt gtccgtgcct
     4741 gcctgcgtgc gtgcgtgcat gcgtgcgagt gaggtgttta cagactcagc atcccgagat
     4801 ttccgggagc ttcacaagtc tcctaacttt actgcctcgt agtcttgagg tgcagccacc
     4861 taatcccgaa tccgcctttg ctgcagagca ggttacacag tataaacctg cttaccccag
     4921 tataaacctg ttcacaaatc tacccctggg tgctggaagg cgcacagagc aaaagctggg
     4981 tttcacatgg ctcagctaaa gccatgaggg agtccgtaca gggagaactg tgtcctgtgg
     5041 atctcaccgc ggaatgatgg gacccagcgt ggaagcagtg tcaagtcgga ggcttggaca
     5101 aatacacacc cggtgaggga tgacagactc tggggctccc ctatacctag cactcagtgt
     5161 ggtagtcccc tgttctctct cgcctgacac ttccggaatg caagagcaga atcttggggg
     5221 ctttgctatc ctgacgtgcc agaaatggga cattctcatt cctggttcat ctctatgcat
     5281 ctttgaaata aaccaaaatg taaaccttgc tacttttttt ttttcaaaga atcatctctg
     5341 aaaagaatta ctgtaaacca atgcctatag tcattctacc aaaataaaca ggattttcac
     5401 tgtg
//
