LOCUS       XM_039088794            5268 bp    mRNA    linear   ROD 11-JUN-2023
DEFINITION  PREDICTED: Rattus norvegicus holocarboxylase synthetase (Hlcs),
            transcript variant X4, mRNA.
ACCESSION   XM_039088794
VERSION     XM_039088794.1
DBLINK      BioProject: PRJNA677964
KEYWORDS    RefSeq.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_051346.1) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            [WARNING] On Feb 22, 2024 this sequence was replaced by
            XM_039088794.2.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Updated annotation
            Annotation Name             :: GCF_015227675.2-RS_2023_06
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.1
            Annotation Method           :: Best-placed RefSeq; Gnomon;
                                           cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 06/06/2023
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5268
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN/NHsdMcwi"
                     /db_xref="taxon:10116"
                     /chromosome="11"
                     /sex="male"
                     /tissue_type="kidney"
                     /geo_loc_name="USA: Wisconsin, Milwaukee, Medical College
                     of Wisconsin"
                     /collection_date="2019-03-08"
                     /collected_by="Rebecca Schilling"
     gene            1..5268
                     /gene="Hlcs"
                     /note="holocarboxylase synthetase; Derived by automated
                     computational analysis using gene prediction method:
                     Gnomon. Supporting evidence includes similarity to: 27
                     ESTs, 2 long SRA reads, 3 Proteins, and 100% coverage of
                     the annotated genomic feature by RNAseq alignments,
                     including 20 samples with support for all annotated
                     introns"
                     /db_xref="GeneID:288240"
                     /db_xref="RGD:1565360"
     CDS             314..2485
                     /gene="Hlcs"
                     /codon_start=1
                     /product="biotin--protein ligase isoform X1"
                     /protein_id="XP_038944722.1"
                     /db_xref="GeneID:288240"
                     /db_xref="RGD:1565360"
                     /translation="MEDRLQMDNGLIAQKIVSVHLKDPALKELSKASNKQVQAPLPSP
                     EASLEAQPAQGVMEQAGQGDWKAAGEGPSPQRRGCAPVSESAADGDPGQGSTELCQLH
                     LSSCHECLELENSTIESVRCASAENIPDLPHDCSSSVEGTAGELCPERKGKRVNISGK
                     APNILLYVGSGSEEALGQLQQVRSVLTDCVDTDSYTLYHLLEDSALRDPWPDNCLLLV
                     IASRDPIPKGIHHRFMAYLSQGGKVLGLSSSFTFGDLRVARRDVLRNTAQNLVFSKAD
                     GSEVRLSVLSSGYVYEEGPSLGRLQGHLENEDKDKMIVHVPFGTHGGEAILCQVHLEL
                     PPSAPLVQTTDDFNVLKSSNTRRLEVLKEILTALGLSCDAPQAPALTPLYLLLAAEEI
                     QGPFMQWLARHVDPEGVIKSSKLSLKFVSSYTSEAEITPSSIPVVTDPEGFSSEHFNL
                     ETYRQNLQSTRLGRVILFAEVTSTTMSLLDGLMFEMPQEMGLIAIAVRQTQGKGRGPN
                     AWLSPVGCALSTLLVSIPLRSQLGQRIPFVQHLMSLAVVEAVRSIPEYEDINLRVKWP
                     NDIYYSDLMKIGGVLVNSTLVGETFYILIGCGFNVTNSNPTICINDLIEEHNKQNKAG
                     LKPLQADCLIARAVTVLEKLIDRFQDQGPDGVLPLYYKYWAHSGQQVRLGSSEGPAVS
                     IVGLDDSGFLQVHQEGGGVVSVHPDGNSFDMLRNLIVPKRP"
ORIGIN      
        1 agcgcgattg gctttgcagg ggacggggag ctctggatcc agattgcagg ggcaggctgc
       61 agcctggcgg cgcgttcccg aggcgcgggc tgcttggcgc agtgcgcatt cccgcgcgct
      121 gctcgccgcg gccgccaggg ggcgcagtgg ggtctcagcg cgaccaccct gcagagcggg
      181 cggccgcgcc ccaggctacg aggttgtaaa atggtcagac tgctgtttgc cgttagcttg
      241 ccgccctggg gatccctacc aactaattgc taaggcaagc gtggacaact tcagcaagct
      301 gggggtggcc ttcatggagg acaggctcca gatggataac ggcctgatag cccagaagat
      361 cgtttcagtt cacttaaagg accctgctct gaaggaactc agcaaggcct ccaacaaaca
      421 agtgcaggcc ccactcccga gcccagaggc ttccctggag gcccagcctg cgcaaggtgt
      481 catggagcaa gctggtcaag gtgactggaa ggctgctggt gaaggccctt ccccacagag
      541 gagaggctgt gcccctgtaa gtgagtctgc tgcagatgga gatccagggc aaggctccac
      601 tgagctctgt cagcttcacc tgtccagctg ccacgagtgt ttggaacttg agaacagcac
      661 catcgagtcg gtgcggtgtg cgtctgcaga gaacattcca gatcttcccc atgattgcag
      721 cagcagtgtg gagggtactg cgggcgaact ctgccctgaa cggaaaggga agagagtcaa
      781 catctcggga aaggcgccca acatcctgct gtacgtgggc tctggttccg aggaagccct
      841 gggccagctc cagcaggtgc gatctgtcct gacagactgt gtggacacgg acagctacac
      901 tctctaccac ctgctggaag acagtgctct gagggacccg tggccagaca actgcctcct
      961 gttagtcatt gccagcaggg accctatccc caaaggcata caccacaggt tcatggccta
     1021 tctttctcag ggagggaagg tgctgggcct gtcttcctcc ttcaccttcg gtgaccttcg
     1081 ggtggccagg agagacgtgc tacggaacac agcccagaac ttggttttct ctaaggctga
     1141 tgggagtgag gtacggctca gtgtcctgag cagcggctac gtttatgagg aaggcccgag
     1201 cctcggccgg ctccagggcc acctggagaa tgaagacaag gataaaatga ttgtgcatgt
     1261 gccttttgga acccacggag gtgaagccat tctctgccag gtgcatctgg agctcccccc
     1321 aagcgctccc ctggtgcaga ccacagacga ctttaatgtg ctcaaatcca gcaacacgag
     1381 aagactcgag gtccttaagg agatcctgac tgcgctcggt ctgagctgtg acgctccaca
     1441 ggctcctgcc ttaacaccac tgtacttact actggctgcc gaggaaatcc agggtccgtt
     1501 tatgcagtgg cttgcaaggc atgtggatcc tgaaggagtc ataaagtcca gcaagctctc
     1561 ccttaagttc gtctcctcct acacatctga agcagagatc accccgtctt ccatccctgt
     1621 ggtcaccgac ccggaaggct tctcatcaga gcactttaac ctagagacct atcgacagaa
     1681 tctgcagagc acgcgcctcg ggagagtaat tctgtttgca gaagtgacct ctacgaccat
     1741 gagtctcctg gatgggttga tgtttgagat gccgcaggaa atgggcttaa tcgccattgc
     1801 agttcggcag acccagggca aaggaagggg cccgaatgcc tggctgagcc ccgtgggatg
     1861 tgccctttcc actctgctag tctccatccc cctaaggtca caactgggac aaagaattcc
     1921 atttgtccag catttgatgt cactggccgt ggtggaggca gtgcggtcta tcccggagta
     1981 tgaggatatc aacttacgag tgaagtggcc caacgatatt tattacagcg atctcatgaa
     2041 gatcggcggg gttctggtta attcaacact tgtgggagaa acattttata tcctcattgg
     2101 ctgtggattc aatgtgacta acagcaatcc taccatatgt atcaatgacc tcatcgaaga
     2161 acacaataag cagaacaagg caggcctgaa gcccttacaa gccgactgtc tcatagccag
     2221 ggctgtgacc gtgctggaga aactgattga caggtttcag gaccaagggc ccgacggcgt
     2281 tctccccctg tattataaat actgggcgca cagtggtcag caagtccgcc tgggcagcag
     2341 cgaggggcca gcggtgtcta tcgtgggtct tgatgattcc gggttcctgc aggtccacca
     2401 agagggtggt ggggttgtga gcgtgcatcc agatggcaac tcctttgaca tgctgaggaa
     2461 cctcattgtc cccaagcgac cgtaaatgcc gcctccacat ccccagacag ctgggggttg
     2521 ctgctgtgca gcagtggtgg cggcagcatc agggaaactg ctcccaggat gtcctgggag
     2581 gtcagagcag gctctgcttg ctctgctgtc tctgcctgcc tagggcctgg gaaactcatg
     2641 cagtcagacc agcgctgttg ttaactcttt aggttgcggg ttctccgccc ccacccccgt
     2701 cccacccctg ggattgggtt tttgtttttt atctctgtgg ttttattggg tgtatgaagg
     2761 ggcatctggt gaggactgat ggataggtga agaagaatca gtttagctta ggagtttagt
     2821 ttgccttgcg tgtgtagaag agctcttccc cacatggcct ggatctgtgt gtactggggg
     2881 agaatgggga gcaagggggt gggaatagcg atgagattat acaaataccc caaggagtga
     2941 aggccgttag ccgtctgctg ttaaaacgag ggaccccaca gctcaggtga gtgctgagtg
     3001 ctgtcccaga atgcacaggc attaacttac accttgggtt gttgctggtt ttcttagtca
     3061 ggttgggctt cttgtagtag cctggaacac aggactgaag gatggccgca gagacgctgc
     3121 tagccagtgt ggtccccttt ccagggtcag atgtgtcacg tatagatgtt taaaccatga
     3181 tggtagcttc cttggaggca gaggcttgcc caggctgatg gagatatggg ctcactgtct
     3241 ggcccacaca agggagggtt cttggaactt ttaaacccat tagtgacagg ctctgtttgt
     3301 tagaagtggc tctgcacccc atcagcctgt gtcctcaagc aggagactgc agcactgtac
     3361 tgtaaagtca tgattctctg cccccgattt cctaacgtca ggaaagagac agagattgac
     3421 acaaactgcc atcagtttgg agagatatat gtgcatgcag tgtgtgtgtg tgtgtgtgtg
     3481 tgtgtgtatg tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg tgtgtgttta aaaggagatg
     3541 ttcaaaattt gatgtgctaa aagtctccgt ggaagaacaa aggagaggaa gcctttctag
     3601 gtttgtgctt ccagatgtgc tccatgcttt tccatgtacc tccggtctct gtggagcttg
     3661 tgaatcatac catcgctgcc cagcctaaaa gaaccagttc ctgctgcact cctgcttcct
     3721 gccacttcca ctgaagtggg tggaaaggaa acgatggaac agaacactta gctttgaaca
     3781 ggatcctcgt cacaaacgag tcccatcttt acccagcttc tacagtcagg cccaccccag
     3841 aaagcagagt tttcagtacg gaaacccagc caccttcagc ctagcacaga cagccccatt
     3901 tgaaccaaaa atagcagggt gaactacgtt gagggaatgc aacattaaaa gaacttgaaa
     3961 gtaacttctc tggcaaataa gactttgatt aaatcttttc tcaaaagaaa aagataaatg
     4021 tataaatttt agttccggta ttttttttat tcatgcgagt ttaaataaca ctgttcacca
     4081 cccaaaatta caaaaatgac gggccctcga gggagcatct gtaaaatatc atcaagaacg
     4141 aagatctcca ggcttgatgt tgcaaagctt cgtccctcag accttgtaat ataaggccgt
     4201 tcctctccca gggccaccat cactacattt acatttctat gggggattat ttaatgaaaa
     4261 acatgttacg gtttaggctg aaggcctatt ctggatagtt ctgctttggg gaaaaatgtt
     4321 atcatttaat ttcctttctg taagttaaaa ctaatgaagt gtggtcctgt cagagcagac
     4381 agagatgctc cgggcatgcc gattgggtgt tcttgcttat gcagacctgg cactcctggt
     4441 cttgagagac aaaacggcct gaagccgatg ctgtatctcc cattagcctg actttgcctc
     4501 acatgaacca aagacttcaa actccatatc gtagagagca ttctgctgtc gaaggtgtga
     4561 aggctgccac tgtacctgct gtgtgcgtgt gtgtgtccgt gcctgcctgc gtgcgtgcgt
     4621 gcatgcgtgc gagtgaggtg tttacagact cagcatcccg agatttccgg gagcttcaca
     4681 agtctcctaa ctttactgcc tcgtagtctt gaggtgcagc cacctaatcc cgaatccgcc
     4741 tttgctgcag agcaggttac acagtataaa cctgcttacc ccagtataaa cctgttcaca
     4801 aatctacccc tgggtgctgg aaggcgcaca gagcaaaagc tgggtttcac atggctcagc
     4861 taaagccatg agggagtccg tacagggaga actgtgtcct gtggatctca ccgcggaatg
     4921 atgggaccca gcgtggaagc agtgtcaagt cggaggcttg gacaaataca cacccggtga
     4981 gggatgacag actctggggc tcccctatac ctagcactca gtgtggtagt cccctgttct
     5041 ctctcgcctg acacttccgg aatgcaagag cagaatcttg ggggctttgc tatcctgacg
     5101 tgccagaaat gggacattct cattcctggt tcatctctat gcatctttga aataaaccaa
     5161 aatgtaaacc ttgctacttt ttttttttca aagaatcatc tctgaaaaga attactgtaa
     5221 accaatgcct atagtcattc taccaaaata aacaggattt tcactgtg
//
