LOCUS       XM_039088793            5456 bp    mRNA    linear   ROD 11-JUN-2023
DEFINITION  PREDICTED: Rattus norvegicus holocarboxylase synthetase (Hlcs),
            transcript variant X3, mRNA.
ACCESSION   XM_039088793
VERSION     XM_039088793.1
DBLINK      BioProject: PRJNA677964
KEYWORDS    RefSeq.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_051346.1) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            [WARNING] On Jan 9, 2024 this sequence was replaced by
            NM_001427024.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Updated annotation
            Annotation Name             :: GCF_015227675.2-RS_2023_06
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.1
            Annotation Method           :: Best-placed RefSeq; Gnomon;
                                           cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 06/06/2023
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5456
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN/NHsdMcwi"
                     /db_xref="taxon:10116"
                     /chromosome="11"
                     /sex="male"
                     /tissue_type="kidney"
                     /geo_loc_name="USA: Wisconsin, Milwaukee, Medical College
                     of Wisconsin"
                     /collection_date="2019-03-08"
                     /collected_by="Rebecca Schilling"
     gene            1..5456
                     /gene="Hlcs"
                     /note="holocarboxylase synthetase; Derived by automated
                     computational analysis using gene prediction method:
                     Gnomon. Supporting evidence includes similarity to: 27
                     ESTs, 2 long SRA reads, 5 Proteins, and 100% coverage of
                     the annotated genomic feature by RNAseq alignments,
                     including 123 samples with support for all annotated
                     introns"
                     /db_xref="GeneID:288240"
                     /db_xref="RGD:1565360"
     CDS             61..2673
                     /gene="Hlcs"
                     /codon_start=1
                     /product="biotin--protein ligase isoform X3"
                     /protein_id="XP_038944721.1"
                     /db_xref="GeneID:288240"
                     /db_xref="RGD:1565360"
                     /translation="MLITLCYLYLWARWGRRPAALVLSTVRRLRASRCSFTFCRAAAQ
                     PGGARVCLSRGGRVFCVGESQSVDDLNKWALFLVSPFILEAEHIAFVTESIWVQGTSF
                     QEPSSSETVVKWSDCCLPLACRPGDPYQLIAKASVDNFSKLGVAFMEDRLQMDNGLIA
                     QKIVSVHLKDPALKELSKASNKQVQAPLPSPEASLEAQPAQGVMEQAGQGDWKAAGEG
                     PSPQRRGCAPVSESAADGDPGQGSTELCQLHLSSCHECLELENSTIESVRCASAENIP
                     DLPHDCSSSVEGTAGELCPERKGKRVNISGKAPNILLYVGSGSEEALGQLQQVRSVLT
                     DCVDTDSYTLYHLLEDSALRDPWPDNCLLLVIASRDPIPKGIHHRFMAYLSQGGKVLG
                     LSSSFTFGDLRVARRDVLRNTAQNLVFSKADGSEVRLSVLSSGYVYEEGPSLGRLQGH
                     LENEDKDKMIVHVPFGTHGGEAILCQVHLELPPSAPLVQTTDDFNVLKSSNTRRLEVL
                     KEILTALGLSCDAPQAPALTPLYLLLAAEEIQGPFMQWLARHVDPEGVIKSSKLSLKF
                     VSSYTSEAEITPSSIPVVTDPEGFSSEHFNLETYRQNLQSTRLGRVILFAEVTSTTMS
                     LLDGLMFEMPQEMGLIAIAVRQTQGKGRGPNAWLSPVGCALSTLLVSIPLRSQLGQRI
                     PFVQHLMSLAVVEAVRSIPEYEDINLRVKWPNDIYYSDLMKIGGVLVNSTLVGETFYI
                     LIGCGFNVTNSNPTICINDLIEEHNKQNKAGLKPLQADCLIARAVTVLEKLIDRFQDQ
                     GPDGVLPLYYKYWAHSGQQVRLGSSEGPAVSIVGLDDSGFLQVHQEGGGVVSVHPDGN
                     SFDMLRNLIVPKRP"
ORIGIN      
        1 cctcgcttcc ggccggggcg gggcctggcg caggccgggg cgcgggcgcc ggcgccggcc
       61 atgctcatca cgctgtgtta cctgtacctg tgggcgcgct gggggcgccg gccggccgca
      121 ctggtgctct ccacggtgcg gcggctgcgc gcctcgcgct gctccttcac cttctgccgc
      181 gcggccgcgc agcccggggg cgcgcgcgtg tgcctgagcc gcggcggccg cgtcttctgc
      241 gtcggcgaga gccagtctgt tgatgacttg aacaaatggg ccctgtttct cgtgtccccc
      301 ttcatacttg aggcagaaca tatagcattt gtgacagaaa gcatttgggt acaaggcacc
      361 agtttccagg agccatcgtc ttcagaaacc gttgtaaaat ggtcagactg ctgtttgccg
      421 ttagcttgcc gccctgggga tccctaccaa ctaattgcta aggcaagcgt ggacaacttc
      481 agcaagctgg gggtggcctt catggaggac aggctccaga tggataacgg cctgatagcc
      541 cagaagatcg tttcagttca cttaaaggac cctgctctga aggaactcag caaggcctcc
      601 aacaaacaag tgcaggcccc actcccgagc ccagaggctt ccctggaggc ccagcctgcg
      661 caaggtgtca tggagcaagc tggtcaaggt gactggaagg ctgctggtga aggcccttcc
      721 ccacagagga gaggctgtgc ccctgtaagt gagtctgctg cagatggaga tccagggcaa
      781 ggctccactg agctctgtca gcttcacctg tccagctgcc acgagtgttt ggaacttgag
      841 aacagcacca tcgagtcggt gcggtgtgcg tctgcagaga acattccaga tcttccccat
      901 gattgcagca gcagtgtgga gggtactgcg ggcgaactct gccctgaacg gaaagggaag
      961 agagtcaaca tctcgggaaa ggcgcccaac atcctgctgt acgtgggctc tggttccgag
     1021 gaagccctgg gccagctcca gcaggtgcga tctgtcctga cagactgtgt ggacacggac
     1081 agctacactc tctaccacct gctggaagac agtgctctga gggacccgtg gccagacaac
     1141 tgcctcctgt tagtcattgc cagcagggac cctatcccca aaggcataca ccacaggttc
     1201 atggcctatc tttctcaggg agggaaggtg ctgggcctgt cttcctcctt caccttcggt
     1261 gaccttcggg tggccaggag agacgtgcta cggaacacag cccagaactt ggttttctct
     1321 aaggctgatg ggagtgaggt acggctcagt gtcctgagca gcggctacgt ttatgaggaa
     1381 ggcccgagcc tcggccggct ccagggccac ctggagaatg aagacaagga taaaatgatt
     1441 gtgcatgtgc cttttggaac ccacggaggt gaagccattc tctgccaggt gcatctggag
     1501 ctccccccaa gcgctcccct ggtgcagacc acagacgact ttaatgtgct caaatccagc
     1561 aacacgagaa gactcgaggt ccttaaggag atcctgactg cgctcggtct gagctgtgac
     1621 gctccacagg ctcctgcctt aacaccactg tacttactac tggctgccga ggaaatccag
     1681 ggtccgttta tgcagtggct tgcaaggcat gtggatcctg aaggagtcat aaagtccagc
     1741 aagctctccc ttaagttcgt ctcctcctac acatctgaag cagagatcac cccgtcttcc
     1801 atccctgtgg tcaccgaccc ggaaggcttc tcatcagagc actttaacct agagacctat
     1861 cgacagaatc tgcagagcac gcgcctcggg agagtaattc tgtttgcaga agtgacctct
     1921 acgaccatga gtctcctgga tgggttgatg tttgagatgc cgcaggaaat gggcttaatc
     1981 gccattgcag ttcggcagac ccagggcaaa ggaaggggcc cgaatgcctg gctgagcccc
     2041 gtgggatgtg ccctttccac tctgctagtc tccatccccc taaggtcaca actgggacaa
     2101 agaattccat ttgtccagca tttgatgtca ctggccgtgg tggaggcagt gcggtctatc
     2161 ccggagtatg aggatatcaa cttacgagtg aagtggccca acgatattta ttacagcgat
     2221 ctcatgaaga tcggcggggt tctggttaat tcaacacttg tgggagaaac attttatatc
     2281 ctcattggct gtggattcaa tgtgactaac agcaatccta ccatatgtat caatgacctc
     2341 atcgaagaac acaataagca gaacaaggca ggcctgaagc ccttacaagc cgactgtctc
     2401 atagccaggg ctgtgaccgt gctggagaaa ctgattgaca ggtttcagga ccaagggccc
     2461 gacggcgttc tccccctgta ttataaatac tgggcgcaca gtggtcagca agtccgcctg
     2521 ggcagcagcg aggggccagc ggtgtctatc gtgggtcttg atgattccgg gttcctgcag
     2581 gtccaccaag agggtggtgg ggttgtgagc gtgcatccag atggcaactc ctttgacatg
     2641 ctgaggaacc tcattgtccc caagcgaccg taaatgccgc ctccacatcc ccagacagct
     2701 gggggttgct gctgtgcagc agtggtggcg gcagcatcag ggaaactgct cccaggatgt
     2761 cctgggaggt cagagcaggc tctgcttgct ctgctgtctc tgcctgccta gggcctggga
     2821 aactcatgca gtcagaccag cgctgttgtt aactctttag gttgcgggtt ctccgccccc
     2881 acccccgtcc cacccctggg attgggtttt tgttttttat ctctgtggtt ttattgggtg
     2941 tatgaagggg catctggtga ggactgatgg ataggtgaag aagaatcagt ttagcttagg
     3001 agtttagttt gccttgcgtg tgtagaagag ctcttcccca catggcctgg atctgtgtgt
     3061 actgggggag aatggggagc aagggggtgg gaatagcgat gagattatac aaatacccca
     3121 aggagtgaag gccgttagcc gtctgctgtt aaaacgaggg accccacagc tcaggtgagt
     3181 gctgagtgct gtcccagaat gcacaggcat taacttacac cttgggttgt tgctggtttt
     3241 cttagtcagg ttgggcttct tgtagtagcc tggaacacag gactgaagga tggccgcaga
     3301 gacgctgcta gccagtgtgg tcccctttcc agggtcagat gtgtcacgta tagatgttta
     3361 aaccatgatg gtagcttcct tggaggcaga ggcttgccca ggctgatgga gatatgggct
     3421 cactgtctgg cccacacaag ggagggttct tggaactttt aaacccatta gtgacaggct
     3481 ctgtttgtta gaagtggctc tgcaccccat cagcctgtgt cctcaagcag gagactgcag
     3541 cactgtactg taaagtcatg attctctgcc cccgatttcc taacgtcagg aaagagacag
     3601 agattgacac aaactgccat cagtttggag agatatatgt gcatgcagtg tgtgtgtgtg
     3661 tgtgtgtgtg tgtgtatgtg tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg tgtgtttaaa
     3721 aggagatgtt caaaatttga tgtgctaaaa gtctccgtgg aagaacaaag gagaggaagc
     3781 ctttctaggt ttgtgcttcc agatgtgctc catgcttttc catgtacctc cggtctctgt
     3841 ggagcttgtg aatcatacca tcgctgccca gcctaaaaga accagttcct gctgcactcc
     3901 tgcttcctgc cacttccact gaagtgggtg gaaaggaaac gatggaacag aacacttagc
     3961 tttgaacagg atcctcgtca caaacgagtc ccatctttac ccagcttcta cagtcaggcc
     4021 caccccagaa agcagagttt tcagtacgga aacccagcca ccttcagcct agcacagaca
     4081 gccccatttg aaccaaaaat agcagggtga actacgttga gggaatgcaa cattaaaaga
     4141 acttgaaagt aacttctctg gcaaataaga ctttgattaa atcttttctc aaaagaaaaa
     4201 gataaatgta taaattttag ttccggtatt ttttttattc atgcgagttt aaataacact
     4261 gttcaccacc caaaattaca aaaatgacgg gccctcgagg gagcatctgt aaaatatcat
     4321 caagaacgaa gatctccagg cttgatgttg caaagcttcg tccctcagac cttgtaatat
     4381 aaggccgttc ctctcccagg gccaccatca ctacatttac atttctatgg gggattattt
     4441 aatgaaaaac atgttacggt ttaggctgaa ggcctattct ggatagttct gctttgggga
     4501 aaaatgttat catttaattt cctttctgta agttaaaact aatgaagtgt ggtcctgtca
     4561 gagcagacag agatgctccg ggcatgccga ttgggtgttc ttgcttatgc agacctggca
     4621 ctcctggtct tgagagacaa aacggcctga agccgatgct gtatctccca ttagcctgac
     4681 tttgcctcac atgaaccaaa gacttcaaac tccatatcgt agagagcatt ctgctgtcga
     4741 aggtgtgaag gctgccactg tacctgctgt gtgcgtgtgt gtgtccgtgc ctgcctgcgt
     4801 gcgtgcgtgc atgcgtgcga gtgaggtgtt tacagactca gcatcccgag atttccggga
     4861 gcttcacaag tctcctaact ttactgcctc gtagtcttga ggtgcagcca cctaatcccg
     4921 aatccgcctt tgctgcagag caggttacac agtataaacc tgcttacccc agtataaacc
     4981 tgttcacaaa tctacccctg ggtgctggaa ggcgcacaga gcaaaagctg ggtttcacat
     5041 ggctcagcta aagccatgag ggagtccgta cagggagaac tgtgtcctgt ggatctcacc
     5101 gcggaatgat gggacccagc gtggaagcag tgtcaagtcg gaggcttgga caaatacaca
     5161 cccggtgagg gatgacagac tctggggctc ccctatacct agcactcagt gtggtagtcc
     5221 cctgttctct ctcgcctgac acttccggaa tgcaagagca gaatcttggg ggctttgcta
     5281 tcctgacgtg ccagaaatgg gacattctca ttcctggttc atctctatgc atctttgaaa
     5341 taaaccaaaa tgtaaacctt gctacttttt ttttttcaaa gaatcatctc tgaaaagaat
     5401 tactgtaaac caatgcctat agtcattcta ccaaaataaa caggattttc actgtg
//
