LOCUS       NM_012735               3633 bp    mRNA    linear   ROD 25-FEB-2025
DEFINITION  Rattus norvegicus hexokinase 2 (Hk2), mRNA.
ACCESSION   NM_012735
VERSION     NM_012735.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 3633)
  AUTHORS   Lin,J., Wang,L., Wu,Y., Xiang,Q., Zhao,Y., Zheng,X., Jiang,S.,
            Sun,Z., Fan,D. and Li,W.
  TITLE     Involvement of DJ-1 in the pathogenesis of intervertebral disc
            degeneration via hexokinase 2-mediated mitophagy
  JOURNAL   Exp Mol Med 56 (3), 747-759 (2024)
   PUBMED   38531963
  REMARK    GeneRIF: Involvement of DJ-1 in the pathogenesis of intervertebral
            disc degeneration via hexokinase 2-mediated mitophagy.
REFERENCE   2  (bases 1 to 3633)
  AUTHORS   Zhang,S., Sun,L., Chen,B., Lin,S., Gu,J., Tan,L. and Lin,M.
  TITLE     Telocytes protect against lung tissue fibrosis through hexokinase
            2-dependent pathway by secreting hepatocyte growth factor
  JOURNAL   Clin Exp Pharmacol Physiol 50 (12), 964-972 (2023)
   PUBMED   37715611
  REMARK    GeneRIF: Telocytes protect against lung tissue fibrosis through
            hexokinase 2-dependent pathway by secreting hepatocyte growth
            factor.
REFERENCE   3  (bases 1 to 3633)
  AUTHORS   Wang,P., Cong,M., Liu,T., Li,Y., Liu,L., Sun,S., Sun,L., Zhu,Z.,
            Ma,H., You,H., Zhang,H. and Jia,J.
  TITLE     FoxA2 inhibits the proliferation of hepatic progenitor cells by
            reducing PI3K/Akt/HK2-mediated glycolysis
  JOURNAL   J Cell Physiol 235 (12), 9524-9537 (2020)
   PUBMED   32495363
  REMARK    GeneRIF: FoxA2 inhibits the proliferation of hepatic progenitor
            cells by reducing PI3K/Akt/HK2-mediated glycolysis.
REFERENCE   4  (bases 1 to 3633)
  AUTHORS   Pereira,G.C., Lee,L., Rawlings,N., Ouwendijk,J., Parker,J.E.,
            Andrienko,T.N., Henley,J.M. and Halestrap,A.P.
  TITLE     Hexokinase II dissociation alone cannot account for changes in
            heart mitochondrial function, morphology and sensitivity to
            permeability transition pore opening following ischemia
  JOURNAL   PLoS One 15 (6), e0234653 (2020)
   PUBMED   32579577
  REMARK    GeneRIF: Hexokinase II dissociation alone cannot account for
            changes in heart mitochondrial function, morphology and sensitivity
            to permeability transition pore opening following ischemia.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 3633)
  AUTHORS   Fan,J.L., Zhu,T.T., Xue,Z.Y., Ren,W.Q., Guo,J.Q., Zhao,H.Y. and
            Zhang,S.L.
  TITLE     lncRNA-XIST protects the hypoxia-induced cardiomyocyte injury
            through regulating the miR-125b-hexokianse 2 axis
  JOURNAL   In Vitro Cell Dev Biol Anim 56 (4), 349-357 (2020)
   PUBMED   32415544
  REMARK    GeneRIF: lncRNA-XIST protects the hypoxia-induced cardiomyocyte
            injury through regulating the miR-125b-hexokianse 2 axis.
REFERENCE   6  (bases 1 to 3633)
  AUTHORS   Mathupala,S.P., Rempel,A. and Pedersen,P.L.
  TITLE     Glucose catabolism in cancer cells. Isolation, sequence, and
            activity of the promoter for type II hexokinase
  JOURNAL   J Biol Chem 270 (28), 16918-16925 (1995)
   PUBMED   7622509
REFERENCE   7  (bases 1 to 3633)
  AUTHORS   Thelen,A.P. and Wilson,J.E.
  TITLE     Complete amino acid sequence of the type II isozyme of rat
            hexokinase, deduced from the cloned cDNA: comparison with a
            hexokinase from novikoff ascites tumor
  JOURNAL   Arch Biochem Biophys 286 (2), 645-651 (1991)
   PUBMED   1897984
REFERENCE   8  (bases 1 to 3633)
  AUTHORS   Ganson,N.J. and Fromm,H.J.
  TITLE     Initial rate and isotope exchange studies of rat skeletal muscle
            hexokinase
  JOURNAL   J Biol Chem 260 (22), 12099-12105 (1985)
   PUBMED   3900069
REFERENCE   9  (bases 1 to 3633)
  AUTHORS   Gregoriou,M., Trayer,I.P. and Cornish-Bowden,A.
  TITLE     Isotope-exchange evidence that glucose 6-phosphate inhibits
            rat-muscle hexokinase II at an allosteric site
  JOURNAL   Eur J Biochem 134 (2), 283-288 (1983)
   PUBMED   6603359
REFERENCE   10 (bases 1 to 3633)
  AUTHORS   Gonzalez,C., Ureta,T., Sanchez,R. and Niemeyer,H.
  TITLE     Multiple molecular forms of ATP:hexose 6-phosphotransferase from
            rat liver
  JOURNAL   Biochem Biophys Res Commun 16 (4), 347-352 (1964)
   PUBMED   5871820
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from M68971.1.
            
            On Aug 30, 2012 this sequence version replaced NM_012735.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: M68971.1, AF027179.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMD00132261,
                                           SAMD00132262 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-3633              M68971.1           2-3634
FEATURES             Location/Qualifiers
     source          1..3633
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10116"
                     /chromosome="4"
                     /map="4q34"
     gene            1..3633
                     /gene="Hk2"
                     /note="hexokinase 2"
                     /db_xref="GeneID:25059"
                     /db_xref="RGD:2797"
     exon            1..259
                     /gene="Hk2"
                     /inference="alignment:Splign:2.1.0"
     CDS             197..2950
                     /gene="Hk2"
                     /EC_number="2.7.1.1"
                     /note="HK II; hexokinase type II; type II hexokinase;
                     hexokinase-B"
                     /codon_start=1
                     /product="hexokinase-2"
                     /protein_id="NP_036867.1"
                     /db_xref="GeneID:25059"
                     /db_xref="RGD:2797"
                     /translation="MIASHMIACLFTELNQNQVQKVDQFLYHMRLSDETLLEISRRFR
                     KEMEKGLGATTHPTAAVKMLPTFVRSTPDGTEHGEFLALDLGGTNFRVLRVRVTDNGL
                     QRVEMENQIYAIPEDIMRGSGTQLFDHIAECLANFMDKLQIKEKKLPLGFTFSFPCHQ
                     TKLDESFLVSWTKGFKSSGVEGRDVVDLIRKAIQRRGDFDIDIVAVVNDTVGTMMTCG
                     YDDQNCEIGLIVGTGSNACYMEEMRHIDMVEGDEGRMCINMEWGAFGDDGTLNDIRTE
                     FDREIDMGSLNPGKQLFEKMISGMYMGELVRLILVKMAKAELLFQGKLSPELLTTGSF
                     ETKDVSDIEEDKDGIEKAYQILMRLGLNPLQEDCVATHRICQIVSTRSASLCAATLAA
                     VLWRIKENKGEERLRSTIGVDGSVYKKHPHFAKRLHKAVRRLVPDCDVRFLRSEDGSG
                     KGAAMVTAVAYRLADQHRARQKTLESLKLSHEQLLEVKRRMKVEMEQGLSKETHAVAP
                     VKMLPTYVCATPDGTEKGDFLALDLGGTNFRVLLVRVRNGKRRGVEMHNKIYSIPQEV
                     MHGTGEELFDHIVQCIADFLEYMGMKGVSLPLGFTFSFPCQQNSLDQSILLKWTKGFK
                     ASGCEGEDVVTLLKEAIHRREEFDLDVVAVVNDTVGTMMTCGYEDPHCEVGLIVGTGS
                     NACYMEEMRNVELVDGEEGRMCVNMEWGAFGDNGCLDDLRTVFDVAVDELSLNPGKQR
                     FEKMISGMYLGEIVRNILIDFTKRGLLFRGRISERLKTRGIFETKFLSQIESDCLALL
                     QVRAILRHLGLESTCDDSIIVKEVCTVVARRAAQLCGAGMAAVVDKIRENRGLDNLKV
                     TVGVDGTLYKLHPHFAKVMHETVRDLAPKCDVSFLESEDGSGKGAALITAVACRIREA
                     GQR"
     exon            260..422
                     /gene="Hk2"
                     /inference="alignment:Splign:2.1.0"
     exon            423..571
                     /gene="Hk2"
                     /inference="alignment:Splign:2.1.0"
     exon            572..691
                     /gene="Hk2"
                     /inference="alignment:Splign:2.1.0"
     exon            692..787
                     /gene="Hk2"
                     /inference="alignment:Splign:2.1.0"
     exon            788..887
                     /gene="Hk2"
                     /inference="alignment:Splign:2.1.0"
     exon            888..1071
                     /gene="Hk2"
                     /inference="alignment:Splign:2.1.0"
     exon            1072..1227
                     /gene="Hk2"
                     /inference="alignment:Splign:2.1.0"
     exon            1228..1461
                     /gene="Hk2"
                     /inference="alignment:Splign:2.1.0"
     exon            1462..1766
                     /gene="Hk2"
                     /inference="alignment:Splign:2.1.0"
     exon            1767..1915
                     /gene="Hk2"
                     /inference="alignment:Splign:2.1.0"
     exon            1916..2035
                     /gene="Hk2"
                     /inference="alignment:Splign:2.1.0"
     exon            2036..2131
                     /gene="Hk2"
                     /inference="alignment:Splign:2.1.0"
     exon            2132..2231
                     /gene="Hk2"
                     /inference="alignment:Splign:2.1.0"
     exon            2232..2415
                     /gene="Hk2"
                     /inference="alignment:Splign:2.1.0"
     exon            2416..2571
                     /gene="Hk2"
                     /inference="alignment:Splign:2.1.0"
     exon            2572..2805
                     /gene="Hk2"
                     /inference="alignment:Splign:2.1.0"
     exon            2806..3633
                     /gene="Hk2"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 ggaaaacctg tttctggaaa cgcgaggccc tcagctggtg agccatcgtg gttaagcttc
       61 tttgtgtggc tcctggagtc tccgatccca gccggacacc cgggcctggt ttcaaagcgg
      121 tcgaactgct ctgcccgctc caccggtagc gctcgagcct cggtttctct actcgacccc
      181 gactcgccgc agcaggatga tcgcctcgca tatgatcgcc tgcttattca cggagctcaa
      241 ccaaaaccaa gtgcagaagg ttgaccaatt tctctaccac atgcgtctct cagatgagac
      301 ccttctggag atttctaggc ggttccggaa ggagatggag aaagggctag gagctaccac
      361 gcaccctaca gcagctgtga aaatgttgcc tacctttgtg aggtcaactc cggatgggac
      421 agaacatggg gagttcctgg ctctggatct tggaggaacc aacttccgtg tgctccgagt
      481 aagggtgacg gacaatggcc tccagagagt ggagatggag aaccagatct acgccatccc
      541 tgaggacatc atgcggggca gtggaaccca gctgtttgac cacatcgccg aatgcctggc
      601 caacttcatg gacaagctac aaatcaaaga gaagaagctc cctctgggtt tcaccttctc
      661 gttcccctgc caccagacaa aactggatga gagttttttg gtctcgtgga ctaaggggtt
      721 caagtccagt ggcgtggaag gcagagatgt ggtggacctg atccggaagg ctatccagcg
      781 cagaggggac tttgacattg acattgtggc cgtggtgaat gacacagttg ggaccatgat
      841 gacttgtggc tatgatgatc agaactgcga gattggtctc attgtgggca ctggcagcaa
      901 cgcctgctac atggaggaaa tgcgtcatat tgacatggtg gagggagatg aggggcgcat
      961 gtgcatcaac atggagtggg gagcctttgg ggacgacggt acactcaatg acatccgaac
     1021 cgagtttgac cgagagatcg acatgggctc gctgaaccct gggaagcagc tgtttgagaa
     1081 gatgattagc gggatgtaca tgggggagct ggtcaggctc atcctggtga agatggccaa
     1141 ggcagagctg ttgttccaag ggaaactcag cccagaactc cttaccactg gctccttcga
     1201 gaccaaagat gtctcggata ttgaagagga taaggatgga atcgagaagg cctaccaaat
     1261 cctgatgcgc ctgggtctga atccattgca ggaggattgt gtggccacgc accgaatctg
     1321 ccagattgtg tccacgcgct cggccagtct gtgcgcagcc accctggccg cggtgctgtg
     1381 gcgaatcaaa gagaacaagg gcgaggagcg acttcgctcc accatcggtg tcgatggctc
     1441 cgtctacaag aaacatcccc attttgccaa gcgtctccat aaggcagtga ggaggctggt
     1501 gcccgactgt gatgtccgct tcctccgctc tgaggatggc agcggcaagg gggctgctat
     1561 ggtgacggcg gtggcttacc gtctggctga ccaacaccgg gcccgccaga agaccctgga
     1621 gtctctgaag ctgagccacg agcagcttct ggaggttaag agaagaatga aggtggaaat
     1681 ggagcagggt ctgagcaagg agacgcatgc ggtcgcccct gtgaagatgc tgcccactta
     1741 cgtgtgtgcc actccagatg gcacagagaa aggagacttc ttggccttgg atcttggagg
     1801 aacaaacttc cgggtcctgc tggtgcgtgt gcgtaatggc aagcggaggg gcgtggagat
     1861 gcataacaag atctactcca tcccacagga ggttatgcat ggcactgggg aagagctctt
     1921 cgaccacatt gtccagtgca ttgcggactt cctggagtac atgggcatga agggcgtgtc
     1981 cctgcctttg ggtttcacat tctccttccc ttgccagcag aacagcctag accagagcat
     2041 cctcctcaag tggacaaagg gattcaaggc atctggctgc gagggtgagg atgtggtcac
     2101 cttgctgaag gaagcgattc accggcgaga ggagtttgac ctggatgtgg ttgccgtggt
     2161 gaatgacaca gttgggacta tgatgacttg tggctacgaa gaccctcact gtgaagttgg
     2221 cctcattgtt ggcaccggaa gcaacgcctg ctacatggaa gagatgcgta atgtggagct
     2281 ggtggacgga gaggagggac ggatgtgtgt caacatggag tggggagcat ttggggacaa
     2341 tggctgcctg gatgacttgc ggaccgtgtt tgatgttgct gtggatgagc tttctctcaa
     2401 ccctggcaaa cagaggttcg agaagatgat cagcggcatg tacttgggag agattgtgcg
     2461 caacattctc atcgatttca cgaagcgggg gctgctcttc cgaggccgca tctcagagcg
     2521 cctcaagaca aggggaatct ttgaaactaa gttcctgtct cagatagaga gcgactgcct
     2581 agccctgcta caggttcgtg ccatcctgcg ccacctaggg ctggagagca cgtgcgatga
     2641 cagcatcatc gtgaaggagg tgtgcactgt ggttgcccgg cgcgctgcac agctctgtgg
     2701 cgcaggcatg gccgccgtag tggacaagat aagagagaac cgtgggctgg acaacctcaa
     2761 agtgacagtg ggcgtggacg ggactctgta taagcttcat cctcactttg ccaaggtcat
     2821 gcatgagacg gtgagagatc tggctccgaa atgtgacgtg tccttcctgg aatccgagga
     2881 cggcagtggg aagggagcag ctctcatcac tgccgtggcc tgccgcatcc gggaggctgg
     2941 gcagagatag aagcttgggg accagacggg gcttctctgc ttcctcactt ccccgtttta
     3001 aattatgaga agatggaccc cttggcagag aggaccctgt gagactggga cttttgtctc
     3061 tgtatattca ctgtagagtt tggtacccaa tccttggcct tcctgagaat ctgaagtaag
     3121 gagttgttcg cagttcctgc tggtcacctc ctgggatggt cctcccccac tctacttcct
     3181 gtacaatcct atgggatggg gacagtcttg agaaaatggt tgcctcagct tggagctgaa
     3241 cctgacattt gtaggtggag aacatcttga aatgtcgatg cttctctcat cagaggtaga
     3301 tgttgggtgg gagggaggag gagagatagg acgtcccctg ccctacgtgg ctgtccctct
     3361 tcaactcaac ccataagcat caccctgccg gttctaaaac atttgctgtc ctgggataga
     3421 ggaatctgaa ccaaagctag agtggacgtc ttcttcaagt gtttgtggct ttacctcaca
     3481 tggctgaatg tgtcatccac gagctacaga ccttggggac aggagtggct ttggtcgctt
     3541 tttagttttt tgttttgttt tgtttttttc ttttttaatt tatgtttgaa atttacttag
     3601 tttaaagcag gtcctctcca ttgtgtaatg gtt
//
