LOCUS       NM_001399619            2869 bp    mRNA    linear   ROD 01-APR-2024
DEFINITION  Rattus norvegicus hepatocyte growth factor-regulated tyrosine
            kinase substrate (Hgs), transcript variant 2, mRNA.
ACCESSION   NM_001399619 XM_006247908
VERSION     NM_001399619.1
KEYWORDS    RefSeq.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 2869)
  AUTHORS   Holleman,J. and Marchese,A.
  TITLE     The ubiquitin ligase deltex-3l regulates endosomal sorting of the G
            protein-coupled receptor CXCR4
  JOURNAL   Mol Biol Cell 25 (12), 1892-1904 (2014)
   PUBMED   24790097
REFERENCE   2  (bases 1 to 2869)
  AUTHORS   Colombo,M., Moita,C., van Niel,G., Kowal,J., Vigneron,J.,
            Benaroch,P., Manel,N., Moita,L.F., Thery,C. and Raposo,G.
  TITLE     Analysis of ESCRT functions in exosome biogenesis, composition and
            secretion highlights the heterogeneity of extracellular vesicles
  JOURNAL   J Cell Sci 126 (Pt 24), 5553-5565 (2013)
   PUBMED   24105262
REFERENCE   3  (bases 1 to 2869)
  AUTHORS   Zhou,R., Kabra,R., Olson,D.R., Piper,R.C. and Snyder,P.M.
  TITLE     Hrs controls sorting of the epithelial Na+ channel between
            endosomal degradation and recycling pathways
  JOURNAL   J Biol Chem 285 (40), 30523-30530 (2010)
   PUBMED   20675381
  REMARK    GeneRIF: Nedd4-2 induces binding of ENaC to Hrs, which mediates the
            sorting decision between ENaC degradation and recycling.
REFERENCE   4  (bases 1 to 2869)
  AUTHORS   Leithe,E., Kjenseth,A., Sirnes,S., Stenmark,H., Brech,A. and
            Rivedal,E.
  TITLE     Ubiquitylation of the gap junction protein connexin-43 signals its
            trafficking from early endosomes to lysosomes in a process mediated
            by Hrs and Tsg101
  JOURNAL   J Cell Sci 122 (Pt 21), 3883-3893 (2009)
   PUBMED   19808888
  REMARK    GeneRIF: Hrs and Tsg101 as crucial regulators of trafficking of
            Cx43 to lysosomes.
REFERENCE   5  (bases 1 to 2869)
  AUTHORS   Rusten,T.E. and Stenmark,H.
  TITLE     How do ESCRT proteins control autophagy?
  JOURNAL   J Cell Sci 122 (Pt 13), 2179-2183 (2009)
   PUBMED   19535733
REFERENCE   6  (bases 1 to 2869)
  AUTHORS   Bean,A.J., Davanger,S., Chou,M.F., Gerhardt,B., Tsujimoto,S. and
            Chang,Y.
  TITLE     Hrs-2 regulates receptor-mediated endocytosis via interactions with
            Eps15
  JOURNAL   J Biol Chem 275 (20), 15271-15278 (2000)
   PUBMED   10809762
REFERENCE   7  (bases 1 to 2869)
  AUTHORS   Stephens,D.J. and Banting,G.
  TITLE     Direct interaction of the trans-Golgi network membrane protein,
            TGN38, with the F-actin binding protein, neurabin
  JOURNAL   J Biol Chem 274 (42), 30080-30086 (1999)
   PUBMED   10514494
REFERENCE   8  (bases 1 to 2869)
  AUTHORS   Ogura,K., Kohno,K. and Tai,T.
  TITLE     Molecular cloning of a rat brain cDNA, with homology to a tyrosine
            kinase substrate, that induces galactosylceramide expression in
            COS-7 cells
  JOURNAL   J Neurochem 71 (5), 1827-1836 (1998)
   PUBMED   9798906
REFERENCE   9  (bases 1 to 2869)
  AUTHORS   Bean,A.J., Seifert,R., Chen,Y.A., Sacks,R. and Scheller,R.H.
  TITLE     Hrs-2 is an ATPase implicated in calcium-regulated secretion
  JOURNAL   Nature 385 (6619), 826-829 (1997)
   PUBMED   9039916
REFERENCE   10 (bases 1 to 2869)
  AUTHORS   Komada,M. and Kitamura,N.
  TITLE     Growth factor-induced tyrosine phosphorylation of Hrs, a novel
            115-kilodalton protein with a structurally conserved putative zinc
            finger domain
  JOURNAL   Mol Cell Biol 15 (11), 6213-6221 (1995)
   PUBMED   7565774
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000010.1.
            
            On Jan 12, 2022 this sequence version replaced XM_006247908.4.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF036344.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760383, SAMEA5760386
                                           [ECO:0000348]
            ##Evidence-Data-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-93                JAXUCZ010000010.1  106238118-106238210
            94-178              JAXUCZ010000010.1  106239434-106239518
            179-254             JAXUCZ010000010.1  106239987-106240062
            255-347             JAXUCZ010000010.1  106240438-106240530
            348-471             JAXUCZ010000010.1  106241964-106242087
            472-524             JAXUCZ010000010.1  106243601-106243653
            525-593             JAXUCZ010000010.1  106245052-106245120
            594-718             JAXUCZ010000010.1  106245872-106245996
            719-797             JAXUCZ010000010.1  106249374-106249452
            798-896             JAXUCZ010000010.1  106249534-106249632
            897-992             JAXUCZ010000010.1  106249755-106249850
            993-1031            JAXUCZ010000010.1  106250469-106250507
            1032-1175           JAXUCZ010000010.1  106250589-106250732
            1176-1235           JAXUCZ010000010.1  106250810-106250869
            1236-1446           JAXUCZ010000010.1  106251221-106251431
            1447-1619           JAXUCZ010000010.1  106251717-106251889
            1620-1760           JAXUCZ010000010.1  106251962-106252102
            1761-1935           JAXUCZ010000010.1  106252180-106252354
            1936-2069           JAXUCZ010000010.1  106254732-106254865
            2070-2189           JAXUCZ010000010.1  106254944-106255063
            2190-2276           JAXUCZ010000010.1  106255214-106255300
            2277-2869           JAXUCZ010000010.1  106255582-106256174
FEATURES             Location/Qualifiers
     source          1..2869
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="10"
                     /map="10q32.3"
     gene            1..2869
                     /gene="Hgs"
                     /gene_synonym="Hrs"
                     /note="hepatocyte growth factor-regulated tyrosine kinase
                     substrate"
                     /db_xref="GeneID:56084"
                     /db_xref="RGD:69225"
     exon            1..93
                     /gene="Hgs"
                     /gene_synonym="Hrs"
                     /inference="alignment:Splign:2.1.0"
     CDS             57..2387
                     /gene="Hgs"
                     /gene_synonym="Hrs"
                     /note="isoform 2 is encoded by transcript variant 2;
                     SNAP-25-interacting protein Hrs-2; HGF-regulated tyrosine
                     kinase substrate"
                     /codon_start=1
                     /product="hepatocyte growth factor-regulated tyrosine
                     kinase substrate isoform 2"
                     /protein_id="NP_001386548.1"
                     /db_xref="GeneID:56084"
                     /db_xref="RGD:69225"
                     /translation="MGRGSGTFERLLDKATSQLLLETDWESILQICDLIRQGDTQAKY
                     AVNSIKKKVNDKNPHVALYALEVMESVVKNCGQTVHDEVANKQTMEELKELLKRQVEV
                     NVRNKILYLIQAWAHAFRNEPKYKVVQDTYQIMKVEGHVFPEFKESDAMFAAERAPDW
                     VDAEECHRCRVQFGVVTRKHHCRACGQIFCGKCSSKYSTIPKFGIEKEVRVCEPCYEQ
                     LNKKAEGKAASTTELPPEYLTSPLSQQSQLPPKRDETALQEEEELQLALALSQSEAEE
                     KERMRQKSTYTAHPKSEPAPLASSAPPAGSLYSSPVNSSAPLAEDIDPELARYLNRNY
                     WEKKQEEARKSPTPSAPVPLTEPAAQPGEGHTAPNSMVEAPLPETDSQPITSCSGPFS
                     EQYQNGESEESHEQFLKALQNAVSTFVNRMKSNHMRGRSITNDSAVLSLFQSINSTHP
                     QLLELLNRLDERRLYYEGLQDKLAQIRDARGALSALREEHREKLRRAAEEAERQRQIQ
                     LAQKLEIMRQKKQEYLEVQRQLAIQRLQEQEKERQMRLEQQKQTVQMRAQMPAFPLPY
                     AQLQAMPTAGGVLYQPSGPTSFPGTFSPAGSVEGSPMHGVYMSQPAPATGPYPSMPGT
                     TADPSMVSAYMYPAGAPGAQAAPQAQAGPTTNPAYSSYQPTPTPGYQNVASQAPQSLP
                     AISQPPQTSNIGYMGSQPMSMGYQPYNMQNLMTTLPGQDASLPAQQPYITGQQPMYQQ
                     MAPSTGPPQQQPPVAQPPPTQGPPAQGNETQLISFD"
     exon            94..178
                     /gene="Hgs"
                     /gene_synonym="Hrs"
                     /inference="alignment:Splign:2.1.0"
     exon            179..254
                     /gene="Hgs"
                     /gene_synonym="Hrs"
                     /inference="alignment:Splign:2.1.0"
     exon            255..347
                     /gene="Hgs"
                     /gene_synonym="Hrs"
                     /inference="alignment:Splign:2.1.0"
     exon            348..471
                     /gene="Hgs"
                     /gene_synonym="Hrs"
                     /inference="alignment:Splign:2.1.0"
     exon            472..524
                     /gene="Hgs"
                     /gene_synonym="Hrs"
                     /inference="alignment:Splign:2.1.0"
     exon            525..593
                     /gene="Hgs"
                     /gene_synonym="Hrs"
                     /inference="alignment:Splign:2.1.0"
     exon            594..718
                     /gene="Hgs"
                     /gene_synonym="Hrs"
                     /inference="alignment:Splign:2.1.0"
     exon            719..797
                     /gene="Hgs"
                     /gene_synonym="Hrs"
                     /inference="alignment:Splign:2.1.0"
     exon            798..896
                     /gene="Hgs"
                     /gene_synonym="Hrs"
                     /inference="alignment:Splign:2.1.0"
     exon            897..992
                     /gene="Hgs"
                     /gene_synonym="Hrs"
                     /inference="alignment:Splign:2.1.0"
     exon            993..1031
                     /gene="Hgs"
                     /gene_synonym="Hrs"
                     /inference="alignment:Splign:2.1.0"
     exon            1032..1175
                     /gene="Hgs"
                     /gene_synonym="Hrs"
                     /inference="alignment:Splign:2.1.0"
     exon            1176..1235
                     /gene="Hgs"
                     /gene_synonym="Hrs"
                     /inference="alignment:Splign:2.1.0"
     exon            1236..1446
                     /gene="Hgs"
                     /gene_synonym="Hrs"
                     /inference="alignment:Splign:2.1.0"
     exon            1447..1619
                     /gene="Hgs"
                     /gene_synonym="Hrs"
                     /inference="alignment:Splign:2.1.0"
     exon            1620..1760
                     /gene="Hgs"
                     /gene_synonym="Hrs"
                     /inference="alignment:Splign:2.1.0"
     exon            1761..1935
                     /gene="Hgs"
                     /gene_synonym="Hrs"
                     /inference="alignment:Splign:2.1.0"
     exon            1936..2069
                     /gene="Hgs"
                     /gene_synonym="Hrs"
                     /inference="alignment:Splign:2.1.0"
     exon            2070..2189
                     /gene="Hgs"
                     /gene_synonym="Hrs"
                     /inference="alignment:Splign:2.1.0"
     exon            2190..2276
                     /gene="Hgs"
                     /gene_synonym="Hrs"
                     /inference="alignment:Splign:2.1.0"
     exon            2277..2869
                     /gene="Hgs"
                     /gene_synonym="Hrs"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gcgcgtcagc tccgaaagag ggagcgcccg cggcgtcggt ccggagtggg gtcgccatgg
       61 ggcgaggcag cggcaccttc gagcgtctcc tagacaaagc caccagccag cttctattgg
      121 agacagactg ggagtccatt ctacagatct gcgacctgat ccgtcagggg gacacacaag
      181 caaaatatgc tgtaaactcc atcaagaaga aggtcaatga taagaaccca catgtggctt
      241 tgtatgctct ggaggtgatg gagtctgtgg taaagaactg tggccagaca gtccatgatg
      301 aagtggccaa caaacagacc atggaagaac tgaaggagct gctgaagagg caagtggaag
      361 ttaatgttcg gaacaagatc ttgtacctga tccaggcctg ggcacatgcg ttccggaatg
      421 aacccaagta caaggtggtc caggacacat accagatcat gaaggtagaa ggacatgtct
      481 tccctgagtt taaggagagc gacgccatgt ttgctgctga aagagcccct gactgggtgg
      541 atgctgagga gtgccatcgg tgcagagtac agtttggagt ggtgacccgc aagcatcact
      601 gccgagcgtg tgggcagatc ttttgtggca agtgttcctc caagtactcc accatcccca
      661 agttcggcat tgagaaggaa gtgcgcgtgt gtgagccctg ctatgagcag ctgaacaaga
      721 aggcagaagg gaaagctgcc tctaccactg agctgccccc agagtacctg accagccccc
      781 tgtcacagca gtctcagctg cccccaaagc gggatgagac agcactgcaa gaagaggagg
      841 agctacagct ggcgctggcc ctatcacagt cagaggctga ggagaaggaa aggatgagac
      901 agaagtcaac atacacagcg catccaaagt cagagcctgc gcccttggct tcctctgcac
      961 ccccagctgg tagcctgtac tcctcgcctg tgaactcatc agcacctctg gctgaggaca
     1021 tcgaccctga gcttgcaaga tacctcaacc ggaactactg ggagaagaaa caggaagaag
     1081 ctcggaagag ccccacacca tctgcacctg tgcccctgac cgagccagct gcccagcccg
     1141 gagaaggaca tacagccccc aacagcatgg tagaggcccc tcttccagag acagactctc
     1201 agcccataac ttcctgcagt ggccccttta gtgagcagta ccagaacggg gagtcggagg
     1261 agagccacga gcagttcctc aaggccctgc agaatgcagt cagcactttt gtcaaccgca
     1321 tgaagagcaa ccacatgagg ggccgcagta tcaccaatga ctcggctgtg ctgtccctct
     1381 tccagtccat caatagcaca cacccacagc tgctcgagct gctcaaccgg ctggatgagc
     1441 gcaggctgta ctacgagggg cttcaggaca agctggcaca gatacgtgat gcccgagggg
     1501 ccctgagtgc cctgcgtgaa gagcacaggg agaagctgcg ccgggcagct gaggaggcgg
     1561 agcgtcaacg tcagatccag ctggcacaga agctggagat catgagacaa aagaagcagg
     1621 agtatctgga ggtgcagaga cagctagcta tccagcgtct gcaggaacag gagaaggaac
     1681 ggcagatgcg cctggagcaa cagaagcaga ctgtccagat gcgtgcccag atgcctgcct
     1741 tccccttgcc ttatgcccag ctccaggcta tgcccacagc tgggggtgta ctctaccagc
     1801 cctcaggccc aaccagcttt cctggcacct ttagcccagc aggctcagta gagggctctc
     1861 cgatgcatgg tgtgtatatg agccagccag ccccagccac tggcccctac cccagcatgc
     1921 ctggcaccac agcagatccc agcatggtca gcgcctacat gtacccagca ggtgcccctg
     1981 gggcacaggc agcccctcag gcccaggccg ggcccaccac caaccctgcc tactcctcgt
     2041 accagcctac tccaacccca ggctaccaga atgtggcttc tcaggcccca cagagcctcc
     2101 cagccatctc ccagcctcca cagaccagca acattggcta catggggagc cagccaatgt
     2161 ccatgggcta ccagccatac aacatgcaga atctcatgac cacccttcca ggccaggatg
     2221 cgtctctgcc agcccagcag ccctacatca cagggcagca gcccatgtac cagcagatgg
     2281 cacccagcac tggccctccc cagcagcagc cccctgtggc ccaaccgcca cctacacagg
     2341 gaccgccagc acagggcaat gagacccagc tcatctcctt cgactgacct tgagtctggc
     2401 gctcaccatc cagagtaaca ctacagttct ccagaaacca cttatatgtc taactagcca
     2461 tttcctccca ttactgccct gtagtgtccc ttctgtgagc aaggggtggg ccttcaccct
     2521 tggccctcct ccctgtcctc agtggtctgg ctcctgtcac tggttccctg ctttggtcct
     2581 gatgcagtcc aaccttcccg ggactgggac tctgcaggac aagtaggacc ttttctggag
     2641 aatgcccagc tgtgtcgggg cctgccagag gtgactgctg tggggatggt tagcctggcc
     2701 cagagtccga tgtgaactgt gtggtgtcca ggcatggccc tggtacccag aagaacgatg
     2761 tgacccatgc acagcaaggg tagaagtttc aggcatctct gtctccccta ctccttggat
     2821 gtcatctctc cagtgcagaa cagtgaaaat aaaatgtatt tcaaaattc
//
