LOCUS       NM_001173434            1406 bp    mRNA    linear   ROD 10-FEB-2025
DEFINITION  Rattus norvegicus homeostatic iron regulator (Hfe), transcript
            variant 2, mRNA.
ACCESSION   NM_001173434
VERSION     NM_001173434.1
KEYWORDS    RefSeq.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 1406)
  AUTHORS   Tamosauskaite,J., Atkins,J.L., Pilling,L.C., Kuo,C.L., Kuchel,G.A.,
            Ferrucci,L. and Melzer,D.
  TITLE     Hereditary Hemochromatosis Associations with Frailty, Sarcopenia
            and Chronic Pain: Evidence from 200,975 Older UK Biobank
            Participants
  JOURNAL   J Gerontol A Biol Sci Med Sci 74 (3), 337-342 (2019)
   PUBMED   30657865
REFERENCE   2  (bases 1 to 1406)
  AUTHORS   Smith,D.J., Klein,K., Hartel,G., Wainwright,C.E., Bell,S.C.,
            Anderson,G.J. and Reid,D.W.
  TITLE     Mutations in the HFE gene can be associated with increased lung
            disease severity in cystic fibrosis
  JOURNAL   Gene 683, 12-17 (2019)
   PUBMED   30291871
REFERENCE   3  (bases 1 to 1406)
  AUTHORS   Pilling,L.C., Tamosauskaite,J., Jones,G., Wood,A.R., Jones,L.,
            Kuo,C.L., Kuchel,G.A., Ferrucci,L. and Melzer,D.
  TITLE     Common conditions associated with hereditary haemochromatosis
            genetic variants: cohort study in UK Biobank
  JOURNAL   BMJ 364, k5222 (2019)
   PUBMED   30651232
  REMARK    Erratum:[BMJ. 2019 Oct 23;367:l6157. doi: 10.1136/bmj.l6157.. PMID:
            31645331]
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 1406)
  AUTHORS   Frydlova,J., Rogalsky,D.W., Truksa,J., Traeger,L.,
            Steinbicker,A.U., Vokurka,M. and Krijt,J.
  TITLE     Liver HFE protein content is posttranscriptionally decreased in
            iron-deficient mice and rats
  JOURNAL   Am J Physiol Gastrointest Liver Physiol 315 (4), G560-G568 (2018)
   PUBMED   29927322
REFERENCE   5  (bases 1 to 1406)
  AUTHORS   Lee,N.P., Wu,H., Ng,K.T.P., Luo,R., Lam,T.W., Lo,C.M. and Man,K.
  TITLE     Transcriptome Analysis of Acute Phase Liver Graft Injury in Liver
            Transplantation
  JOURNAL   Biomedicines 6 (2), 41 (2018)
   PUBMED   29642405
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 1406)
  AUTHORS   Sanchez,M., Queralt,R., Bruguera,M., Rodes,J. and Oliva,R.
  TITLE     Cloning, sequencing and characterization of the rat hereditary
            hemochromatosis promoter: comparison of the human, mouse and rat
            HFE promoter regions
  JOURNAL   Gene 225 (1-2), 77-87 (1998)
   PUBMED   9931446
REFERENCE   7  (bases 1 to 1406)
  AUTHORS   Lebron,J.A., Bennett,M.J., Vaughn,D.E., Chirino,A.J., Snow,P.M.,
            Mintier,G.A., Feder,J.N. and Bjorkman,P.J.
  TITLE     Crystal structure of the hemochromatosis protein HFE and
            characterization of its interaction with transferrin receptor
  JOURNAL   Cell 93 (1), 111-123 (1998)
   PUBMED   9546397
REFERENCE   8  (bases 1 to 1406)
  AUTHORS   Feder,J.N., Penny,D.M., Irrinki,A., Lee,V.K., Lebron,J.A.,
            Watson,N., Tsuchihashi,Z., Sigal,E., Bjorkman,P.J. and
            Schatzman,R.C.
  TITLE     The hemochromatosis gene product complexes with the transferrin
            receptor and lowers its affinity for ligand binding
  JOURNAL   Proc Natl Acad Sci U S A 95 (4), 1472-1477 (1998)
   PUBMED   9465039
REFERENCE   9  (bases 1 to 1406)
  AUTHORS   George,D.K., Goldwurm,S., MacDonald,G.A., Cowley,L.L., Walker,N.I.,
            Ward,P.J., Jazwinska,E.C. and Powell,L.W.
  TITLE     Increased hepatic iron concentration in nonalcoholic
            steatohepatitis is associated with increased fibrosis
  JOURNAL   Gastroenterology 114 (2), 311-318 (1998)
   PUBMED   9453491
REFERENCE   10 (bases 1 to 1406)
  AUTHORS   Feder,J.N., Gnirke,A., Thomas,W., Tsuchihashi,Z., Ruddy,D.A.,
            Basava,A., Dormishian,F., Domingo,R. Jr., Ellis,M.C., Fullan,A.,
            Hinton,L.M., Jones,N.L., Kimmel,B.E., Kronmal,G.S., Lauer,P.,
            Lee,V.K., Loeb,D.B., Mapa,F.A., McClelland,E., Meyer,N.C.,
            Mintier,G.A., Moeller,N., Moore,T., Morikang,E., Prass,C.E.,
            Quintana,L., Starnes,S.M., Schatzman,R.C., Brunke,K.J.,
            Drayna,D.T., Risch,N.J., Bacon,B.R. and Wolff,R.K.
  TITLE     A novel MHC class I-like gene is mutated in patients with
            hereditary haemochromatosis
  JOURNAL   Nat Genet 13 (4), 399-408 (1996)
   PUBMED   8696333
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000017.1.
            
            Transcript Variant: This variant (2) lacks an alternate in-frame
            exon compared to variant 1. The resulting protein (isoform 2) is
            shorter compared to isoform 1.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: FQ219597.1, AF176534.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760389, SAMEA5760393
                                           [ECO:0000348]
            ##Evidence-Data-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-171               JAXUCZ010000017.1  41841302-41841472
            172-471             JAXUCZ010000017.1  41845716-41846015
            472-747             JAXUCZ010000017.1  41846954-41847229
            748-858             JAXUCZ010000017.1  41847343-41847453
            859-1406            JAXUCZ010000017.1  41848806-41849353
FEATURES             Location/Qualifiers
     source          1..1406
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="17"
                     /map="17p11"
     gene            1..1406
                     /gene="Hfe"
                     /note="homeostatic iron regulator"
                     /db_xref="GeneID:29199"
                     /db_xref="RGD:2793"
     exon            1..171
                     /gene="Hfe"
                     /inference="alignment:Splign:2.1.0"
     CDS             81..899
                     /gene="Hfe"
                     /note="isoform 2 precursor is encoded by transcript
                     variant 2; hereditary hemochromatosis protein homolog;
                     RT1-CAFE"
                     /codon_start=1
                     /product="hereditary hemochromatosis protein homolog
                     isoform 2 precursor"
                     /protein_id="NP_001166905.1"
                     /db_xref="GeneID:29199"
                     /db_xref="RGD:2793"
                     /translation="MDRSAGLPVRLLLLLLLLLLWSVAPQALRPVTKLRVVPESHILQ
                     VILGCEVHEDNSTSGFWKYGYDGQDHLEFCPKTLNWSAAEPRAWATKMEWEEHRIRAR
                     QSRDYLQRDCPQQLKQVLELQRGVLGQQVPTLVKVTRHWASTGTSLRCQALNFFPQNI
                     TMRWLKDSQPLDAKDVNPENVLPNGDGTYQGWLTLAVAPGEETRFSCQVEHPGLDQPL
                     TATWEPSRSQDMIIGIISGITICAIFFVGILILVLRKRKVSGGTMGDYVLTECE"
     exon            172..471
                     /gene="Hfe"
                     /inference="alignment:Splign:2.1.0"
     exon            472..747
                     /gene="Hfe"
                     /inference="alignment:Splign:2.1.0"
     exon            748..858
                     /gene="Hfe"
                     /inference="alignment:Splign:2.1.0"
     exon            859..1406
                     /gene="Hfe"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 tcagcaatgg ctacagggtg acttcttgga tcctccacgt ttccaggtcc tagtgaaaac
       61 cggtggaccc agctggaggc atggaccgat cagctgggct ccctgtgcgg ctgctattgc
      121 tgctgctgtt gttgctgctg tggtccgtgg ccccgcaggc gctgcggccc gtcacgaagt
      181 tgagagtggt gcctgagtct cacatcctgc aggtgatcct aggatgtgag gtgcatgaag
      241 acaacagtac cagtggcttc tggaaatatg gctacgatgg gcaagatcac cttgaattct
      301 gccccaagac actgaactgg agtgcagccg agccaagggc ctgggccacc aagatggagt
      361 gggaagagca caggatccgt gccagacaga gcagggacta cctgcagagg gactgccccc
      421 agcagctgaa gcaggtcctg gagctccaga gaggggttct gggacagcaa gtgcctactt
      481 tggtgaaagt gactcgccac tgggcctcta cagggacctc tctaaggtgt caggctctga
      541 atttcttccc ccagaacatc actatgaggt ggttgaagga cagccagccc ctagatgcca
      601 aggatgtcaa ccctgagaac gtgctgccaa atggggatgg gacctatcag ggctggctga
      661 ccttggctgt ggcccctgga gaagagacaa ggttcagctg tcaagtggag cacccaggcc
      721 tggatcagcc tctcactgcc acttgggagc cctcacggtc tcaggacatg attattggaa
      781 tcataagtgg gatcaccatt tgtgccatct tctttgttgg aattctgatc ctagtcttaa
      841 ggaaaaggaa ggtttcagga ggaaccatgg gtgactatgt cttaacagag tgtgagtgac
      901 ctgcagcatg cagaagcaca gaagagagaa gactcagcca aagacttgga ggggacacac
      961 ttgctccatt ctagaacaca gctggaccta acacacagaa actgcctgag gactctgccc
     1021 ttagctttcc tgtttgcttt cttaaggtgt tttctccagt taagttcagt tcctgaataa
     1081 tagtgactgc cccagctgca acctctccct tcagaaccag tctcatgatc tttaagctgc
     1141 tacttgcagg catccttcgt tttctgcatc cacctagact tcgtatgtct acttaaaaag
     1201 ccccactaaa tttgggggac acatgattca tttccacatc tgaagaagtt atgaaccttc
     1261 atcctgggat gcacacattc ttgtgccaga atttttcata catatcctag gacccattca
     1321 attgtcattt gagcctctct atctgttagt gactactctg acttctctgc cattggagtg
     1381 ttatggcaat aaagctatga acgtta
//
