LOCUS       NM_133304               4260 bp    mRNA    linear   ROD 05-AUG-2024
DEFINITION  Rattus norvegicus hephaestin (Heph), mRNA.
ACCESSION   NM_133304
VERSION     NM_133304.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 4260)
  AUTHORS   Wang,J., Qu,D., An,J., Yuan,G. and Liu,Y.
  TITLE     Integrated microarray analysis provided novel insights to the
            pathogenesis of glaucoma
  JOURNAL   Mol Med Rep 16 (6), 8735-8746 (2017)
   PUBMED   28990066
REFERENCE   2  (bases 1 to 4260)
  AUTHORS   Drakesmith,H., Nemeth,E. and Ganz,T.
  TITLE     Ironing out Ferroportin
  JOURNAL   Cell Metab 22 (5), 777-787 (2015)
   PUBMED   26437604
  REMARK    Review article
REFERENCE   3  (bases 1 to 4260)
  AUTHORS   Kong,W.N., Wu,Q., Shen,D., Zhao,S.E., Guo,P., Duan,X.L. and
            Chang,Y.Z.
  TITLE     Age-dependent expression of duodenal cytochrome b, divalent metal
            transporter 1, ferroportin 1, and hephaestin in the duodenum of
            rats
  JOURNAL   J Gastroenterol Hepatol 30 (3), 513-520 (2015)
   PUBMED   25318588
  REMARK    GeneRIF: Hepcidin or enterocyte iron levels may be involved in the
            regulation of the age-dependent expression in the duodenum.
REFERENCE   4  (bases 1 to 4260)
  AUTHORS   Vashchenko,G. and Macgillivray,R.T.
  TITLE     Functional role of the putative iron ligands in the ferroxidase
            activity of recombinant human hephaestin
  JOURNAL   J Biol Inorg Chem 17 (8), 1187-1195 (2012)
   PUBMED   22961397
REFERENCE   5  (bases 1 to 4260)
  AUTHORS   Ranganathan,P.N., Lu,Y., Fuqua,B.K. and Collins,J.F.
  TITLE     Immunoreactive hephaestin and ferroxidase activity are present in
            the cytosolic fraction of rat enterocytes
  JOURNAL   Biometals 25 (4), 687-695 (2012)
   PUBMED   22350470
  REMARK    GeneRIF: Cytosolic Heph increased upon iron-deprivation but more
            important, decreased significantly upon copper-deprivation,
            mimicking the response of membrane-bound Heph.
REFERENCE   6  (bases 1 to 4260)
  AUTHORS   Qian,Z.M., Chang,Y.Z., Zhu,L., Yang,L., Du,J.R., Ho,K.P., Wang,Q.,
            Li,L.Z., Wang,C.Y., Ge,X., Jing,N.L., Li,L. and Ke,Y.
  TITLE     Development and iron-dependent expression of hephaestin in
            different brain regions of rats
  JOURNAL   J Cell Biochem 102 (5), 1225-1233 (2007)
   PUBMED   17516501
  REMARK    GeneRIF: Development and iron-dependent expression of Heph in
            different brain regions is reported.
REFERENCE   7  (bases 1 to 4260)
  AUTHORS   Wang,J., Jiang,H. and Xie,J.X.
  TITLE     Ferroportin1 and hephaestin are involved in the nigral iron
            accumulation of 6-OHDA-lesioned rats
  JOURNAL   Eur J Neurosci 25 (9), 2766-2772 (2007)
   PUBMED   17561842
  REMARK    GeneRIF: These results show for the first time that FP1 and HP
            co-localize in the rat brain, and suggest that decreased expression
            of these transporters in the SN can account for the increased iron
            levels.
REFERENCE   8  (bases 1 to 4260)
  AUTHORS   Qian,Z.M., Chang,Y.Z., Leung,G., Du,J.R., Zhu,L., Wang,Q., Niu,L.,
            Xu,Y.J., Yang,L., Ho,K.P. and Ke,Y.
  TITLE     Expression of ferroportin1, hephaestin and ceruloplasmin in rat
            heart
  JOURNAL   Biochim Biophys Acta 1772 (5), 527-532 (2007)
   PUBMED   17383861
  REMARK    GeneRIF: Hephaestin might have a role in heart iron homeostasis.
REFERENCE   9  (bases 1 to 4260)
  AUTHORS   Simovich,M.J., Conrad,M.E., Umbreit,J.N., Moore,E.G.,
            Hainsworth,L.N. and Smith,H.K.
  TITLE     Cellular location of proteins related to iron absorption and
            transport
  JOURNAL   Am J Hematol 69 (3), 164-170 (2002)
   PUBMED   11891802
  REMARK    GeneRIF: cellular locations identified by immunofluorescence
REFERENCE   10 (bases 1 to 4260)
  AUTHORS   Frazer,D.M., Vulpe,C.D., McKie,A.T., Wilkins,S.J., Trinder,D.,
            Cleghorn,G.J. and Anderson,G.J.
  TITLE     Cloning and gastrointestinal expression of rat hephaestin:
            relationship to other iron transport proteins
  JOURNAL   Am J Physiol Gastrointest Liver Physiol 281 (4), G931-G939 (2001)
   PUBMED   11557513
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from AF246120.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF246120.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760393, SAMN06621351
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..4260
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="Sprague-Dawley"
                     /db_xref="taxon:10116"
                     /chromosome="X"
                     /map="Xq22"
     gene            1..4260
                     /gene="Heph"
                     /note="hephaestin"
                     /db_xref="GeneID:117240"
                     /db_xref="RGD:71060"
     exon            1..47
                     /gene="Heph"
                     /inference="alignment:Splign:2.1.0"
     exon            48..248
                     /gene="Heph"
                     /inference="alignment:Splign:2.1.0"
     CDS             82..3555
                     /gene="Heph"
                     /EC_number="1.16.3.1"
                     /codon_start=1
                     /product="hephaestin precursor"
                     /protein_id="NP_579838.1"
                     /db_xref="GeneID:117240"
                     /db_xref="RGD:71060"
                     /translation="MKAGHLLWALLLMHSLCSLPTDGAIRNYYLGIQDIQWNYAPKGR
                     NVITNQTLNNDTVASSFLKSGKNRIGGTYKKTVYKEYSDGTYTNEIAKPAWLGFLGPL
                     LKAEMGDVILIHLKNFASRPYTIHPHGVFYEKDSEGSLYPDGSSGYLKADDSVPPGGS
                     HVYNWSIPEGHAPTEADPACLTWIYHSHVDAPRDIATGLIGPLITCKRGTLDGNSPPQ
                     RKDVDHNFFLLFSVIDENLSWHLDDNIATYCSDPASVDKEDGPFQDSNRMHAINGFVF
                     GNLPELSMCAQKHVAWHLFGMGNEIDVHTAFFHGQTLSIRGHRTDVAHIFPATFVTAE
                     MVPQKSGTWLISCVVNSHLKSGMQAFYKVDSCSMDPPVEQLTGKVRQYFIQAHEIQWD
                     YGPIGHDGRTGKSLREPGSGPDKYFQKSSSRIGGTYWKVRYEAFQDETFQERLHQEEE
                     THLGILGPVIRAEVGDTIQVVFYNRASQPFSIQPHGVFYEKSSEGTVYNDGTSYPKVA
                     KSFEKVTYYWTVPPHAGPTAEDPACLTWMYFSAADPTRDTNSGLVGPLLVCKAGALGE
                     DGKQKGVDKEFFLLFTIFDENESWYNNANQAAGMLDSRLLSEDVEGFEDSNRMHAING
                     FLFSNLPRLDICKGDTVAWHLLGLGTENDVHGVMFEGNTLQLQGMRKSAAMLFPHTFV
                     TAIMQPDNPGIFEIYCQAGSHREAGMQAIYNVSQCSSHQDSPRQHYQASRVYYIMAEE
                     IEWDYCPDRSWELEWYNTSEKDSYGHVFLSNKDGLLGSKYKKAVFREYTDGTFRIPQP
                     RSGPEEHLGILGPLIRGEVGDILTVVFKNKASRPYSIHAHGVLESSTGWPQAAEPGEV
                     LTYQWNIPERSGPGPSDSACVSWIYYSAVDPIKDMYSGLVGPLVICRNGILEPNGGRN
                     DMDREFALLFLIFDENQSWYLKENIATYGPQETSHVNLQDATFLESNKMHAINGKLYA
                     NLRGLTVYQGERVAWYMLAMGQDTDIHTVHFHAESFLYQNGHSYRADVVDLFPGTFEV
                     VEMVASNPGAWLMHCHVTDHVHAGMETIFTVLSHEEHFSTMTTITKEIGKAVILQNIG
                     EGNVKMLGMNIPVKNVEILSSALIAICVVLLLIALALGGVVWYQHRQRKLRRNRRSIL
                     DDSFKLLSLKQ"
     exon            249..493
                     /gene="Heph"
                     /inference="alignment:Splign:2.1.0"
     exon            494..706
                     /gene="Heph"
                     /inference="alignment:Splign:2.1.0"
     exon            707..889
                     /gene="Heph"
                     /inference="alignment:Splign:2.1.0"
     exon            890..1144
                     /gene="Heph"
                     /inference="alignment:Splign:2.1.0"
     exon            1145..1313
                     /gene="Heph"
                     /inference="alignment:Splign:2.1.0"
     exon            1314..1450
                     /gene="Heph"
                     /inference="alignment:Splign:2.1.0"
     exon            1451..1582
                     /gene="Heph"
                     /inference="alignment:Splign:2.1.0"
     exon            1583..1791
                     /gene="Heph"
                     /inference="alignment:Splign:2.1.0"
     exon            1792..1942
                     /gene="Heph"
                     /inference="alignment:Splign:2.1.0"
     exon            1943..2155
                     /gene="Heph"
                     /inference="alignment:Splign:2.1.0"
     exon            2156..2369
                     /gene="Heph"
                     /inference="alignment:Splign:2.1.0"
     exon            2370..2509
                     /gene="Heph"
                     /inference="alignment:Splign:2.1.0"
     exon            2510..2641
                     /gene="Heph"
                     /inference="alignment:Splign:2.1.0"
     exon            2642..2748
                     /gene="Heph"
                     /inference="alignment:Splign:2.1.0"
     exon            2749..2974
                     /gene="Heph"
                     /inference="alignment:Splign:2.1.0"
     exon            2975..3114
                     /gene="Heph"
                     /inference="alignment:Splign:2.1.0"
     exon            3115..3277
                     /gene="Heph"
                     /inference="alignment:Splign:2.1.0"
     exon            3278..3322
                     /gene="Heph"
                     /inference="alignment:Splign:2.1.0"
     exon            3323..4253
                     /gene="Heph"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 tattgtgtgt agttacatgt gactatacct gtgtttcacc agtaaatata ccattccctg
       61 tttctcaaag tactgtgggc catgaaggca ggccatcttc tctgggcttt actgttgatg
      121 cactccttgt gttctctacc aactgacggg gccattcgaa actactacct gggcatccag
      181 gatatacagt ggaactatgc tcccaaagga agaaatgtca tcacaaatca gactctgaac
      241 aatgacacag tggcttccag cttcctaaag tctggcaaaa acaggatagg gggtacttac
      301 aagaagactg tctataagga atacagtgat ggcacataca ccaatgaaat agccaagcct
      361 gcctggttgg gcttcctagg accactgtta aaggctgaga tgggggatgt catcctgatc
      421 cacctgaaga attttgccag ccggccttat accattcacc ctcacggtgt tttctatgag
      481 aaggactcag aaggctcact atacccagat ggatcttctg gttatctgaa agctgatgat
      541 tctgttcccc ctgggggaag ccatgtctac aactggagta ttccagaagg tcatgcaccc
      601 actgaagcag acccagcctg cctcacctgg atttaccact ctcatgtaga tgctccacga
      661 gacattgcaa ctggtctcat tggacctctt atcacctgta aaagagggac tctggatggt
      721 aactccccac ctcaaaggaa ggatgtggac cataatttct tcctcctctt cagtgtgata
      781 gatgagaacc ttagctggca ccttgatgac aacattgcta cttactgctc agaccctgcc
      841 tcagtggaca aagaagatgg accatttcaa gacagcaata ggatgcatgc aatcaatggg
      901 tttgtctttg ggaacttacc cgagttgagc atgtgtgcgc agaagcatgt ggcctggcac
      961 ttgtttggca tgggcaatga gatagatgtc catacagctt tcttccacgg acagacactg
     1021 agtatccgtg gacaccgcac tgatgttgct cacatttttc cagccacctt tgtgactgct
     1081 gagatggtgc cccagaagtc tggaacctgg ttaattagct gtgtggtgaa cagtcacttg
     1141 aaaagtggca tgcaggcctt ctacaaggtt gattcttgtt ccatggaccc acccgtggag
     1201 cagctcacag gcaaagttcg tcagtacttc attcaagccc atgagattca atgggactat
     1261 ggtccaatag ggcatgatgg cagaactggg aagagtttga gagagccagg aagtggccca
     1321 gataagtact tccagaagag ctctagtcga attggaggta cttactggaa agttcgatat
     1381 gaagcctttc aagatgagac attccaggag aggttacatc aggaagaaga aacacatctt
     1441 ggaatactgg gaccagtgat aagggctgaa gtgggtgaca ccatccaggt ggtcttctat
     1501 aaccgtgcct cccagccatt cagcatacaa ccccatggcg tcttttatga gaaaagctct
     1561 gagggcaccg tgtacaatga tggtacttct tatcccaaag ttgccaagtc gtttgaaaaa
     1621 gtaacatact actggacagt tcctccccat gctgggccca ccgctgagga tcctgcctgt
     1681 ctgacctgga tgtacttctc tgctgcagat cccacaagag atacaaattc tggcctggtg
     1741 ggccctctgc tggtgtgcaa ggctggggcc ttgggtgaag atggcaagca gaaaggagtg
     1801 gataaagaat tttttctcct cttcactata tttgatgaga acgagagctg gtacaacaat
     1861 gccaatcagg cagctggtat gttggattcc cgactgctct cagaggatgt cgagggcttc
     1921 gaagactcca atcgaatgca tgctattaat ggatttctgt tctctaacct gcccaggctg
     1981 gacatatgca agggtgatac tgtggcctgg cacctgcttg gcctgggcac agagaatgat
     2041 gtacatgggg ttatgtttga gggcaacact ctacagcttc agggcatgag gaaaagtgca
     2101 gccatgctct ttcctcacac ctttgtgacg gccatcatgc agcctgacaa tcctgggata
     2161 tttgaaatct actgccaagc aggcagccac agagaagcag ggatgcaggc aatttataat
     2221 gtctctcagt gttccagtca tcaagacagc ccacgccaac actaccaagc gtcaagagtc
     2281 tactatatca tggcagagga gatagaatgg gattattgcc ctgacagaag ctgggagctg
     2341 gaatggtata acacgtctga gaaggacagc tatggccatg ttttcttgag caacaaagat
     2401 gggctcctgg gttccaaata taagaaagcg gtattcagag aatacactga tggtacattc
     2461 agaatacctc aaccgaggtc tggaccagag gagcacttag gaattctggg tccacttatc
     2521 agaggagagg ttggtgatat cttgactgtg gtgttcaaga ataaggccag tcgaccatat
     2581 tctatacatg cccatggagt cctagaatct agtactggtt ggccacaagc tgctgagcct
     2641 ggtgaagtac ttacttacca gtggaacatt ccagaaagat ctggtcctgg tcccagtgac
     2701 tctgcttgtg tttcctggat ttattattct gcagtggatc ccataaagga catgtatagt
     2761 ggcctggtag gacccttagt catctgccga aatggtatct tggaacccaa tggaggccgg
     2821 aatgatatgg accgggaatt tgccttgttg tttttgatct ttgatgaaaa ccaatcatgg
     2881 tatctgaaag agaatattgc aacatatgga ccccaagaga caagtcatgt taacttgcag
     2941 gatgcaactt tcctggagag caataaaatg catgccatca atgggaaact ctatgctaac
     3001 ctcaggggtc ttactgtata ccaaggagaa cgagtagcgt ggtacatgct agccatgggc
     3061 caagatactg acattcacac tgtgcacttt catgcagaga gtttcctcta tcagaatggg
     3121 cacagttaca gggcagatgt ggtggatctc ttcccaggaa catttgaagt tgtggagatg
     3181 gtagccagca accctggagc atggctgatg cactgccacg ttactgacca tgttcatgct
     3241 ggcatggaga ccatctttac tgtgttgtct catgaagaac atttcagtac tatgaccacc
     3301 attactaaag agattggaaa agctgtgatt ctgcagaaca ttggagaagg caatgtgaag
     3361 atgctgggca tgaatattcc cgtaaagaac gttgagattt tgtcttctgc tttgattgcc
     3421 atatgtgtgg ttctactgct cattgctctg gcccttggtg gtgtagtctg gtaccagcat
     3481 cgacaaagaa agcttcgtcg caacaggagg tccattcttg atgatagctt caagcttctg
     3541 tctctcaagc agtaagagct gaagcctgaa aatatcttca gaacacatct gagatgtatc
     3601 ccatgttccc agagagctat gtaatctact cttttgtcaa aggggtagag gtagtggaaa
     3661 ggcagaggat ggaatcagaa ttatctgggc gtttccttat ttatttacat gggatccata
     3721 ggttctcact ttttctttaa tttccgtaca acttgagaat taggtaagtt cctttgtata
     3781 caaatttcag cagttagcat atgccatcct tcagtactgc aaagtacaga aattcctaga
     3841 acataatcat tctcacaaaa tagataccaa gaaaaaacta tctaattagg tttctaattc
     3901 caggacctag ataaacaagt acacttttaa ttgaggccaa atgatgagcc acaaaggtaa
     3961 cccagaacta aataaaggac aaagaacata agcataatac aaaatgagag gtagggtggg
     4021 tagtaggatt ccagatttta gcttaattgt ctttgacaat ggactattgg agaaatgaat
     4081 gaatgggttg tggttgtcat gagtacctag aacccctgga accagaagca cctgtaaaag
     4141 agtccttcat gtttttacgc agtggtactg aagagaattt tgagtgtcct ctccatgttc
     4201 gaaatagcaa atcttgttca ctaaaatact tattaaaaga ttgaaatact gaaaaaaaaa
//
