LOCUS       NM_001419838            9024 bp    mRNA    linear   ROD 31-MAR-2024
DEFINITION  Rattus norvegicus HECT domain E3 ubiquitin protein ligase 1
            (Hectd1), transcript variant 1, mRNA.
ACCESSION   NM_001419838 XM_006240086
VERSION     NM_001419838.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 9024)
  AUTHORS   Yang,Y.N., Luo,Y.B., Xu,G., Li,K., Ma,R.L. and Yuan,W.
  TITLE     CircHECTD1 promoted MIRI-associated inflammation via inhibiting
            miR-138-5p and upregulating ROCK2
  JOURNAL   Kaohsiung J Med Sci 39 (7), 675-687 (2023)
   PUBMED   37096660
  REMARK    GeneRIF: CircHECTD1 promoted MIRI-associated inflammation via
            inhibiting miR-138-5p and upregulating ROCK2.
REFERENCE   2  (bases 1 to 9024)
  AUTHORS   Li,Y., Klena,N.T., Gabriel,G.C., Liu,X., Kim,A.J., Lemke,K.,
            Chen,Y., Chatterjee,B., Devine,W., Damerla,R.R., Chang,C., Yagi,H.,
            San Agustin,J.T., Thahir,M., Anderton,S., Lawhead,C., Vescovi,A.,
            Pratt,H., Morgan,J., Haynes,L., Smith,C.L., Eppig,J.T.,
            Reinholdt,L., Francis,R., Leatherbury,L., Ganapathiraju,M.K.,
            Tobita,K., Pazour,G.J. and Lo,C.W.
  TITLE     Global genetic analysis in mice unveils central role for cilia in
            congenital heart disease
  JOURNAL   Nature 521 (7553), 520-524 (2015)
   PUBMED   25807483
REFERENCE   3  (bases 1 to 9024)
  AUTHORS   Sarkar,A.A., Nuwayhid,S.J., Maynard,T., Ghandchi,F., Hill,J.T.,
            Lamantia,A.S. and Zohn,I.E.
  TITLE     Hectd1 is required for development of the junctional zone of the
            placenta
  JOURNAL   Dev Biol 392 (2), 368-380 (2014)
   PUBMED   24855001
REFERENCE   4  (bases 1 to 9024)
  AUTHORS   Sarkar,A.A. and Zohn,I.E.
  TITLE     Hectd1 regulates intracellular localization and secretion of Hsp90
            to control cellular behavior of the cranial mesenchyme
  JOURNAL   J Cell Biol 196 (6), 789-800 (2012)
   PUBMED   22431752
REFERENCE   5  (bases 1 to 9024)
  AUTHORS   Zohn,I.E., Anderson,K.V. and Niswander,L.
  TITLE     The Hectd1 ubiquitin ligase is required for development of the head
            mesenchyme and neural tube closure
  JOURNAL   Dev Biol 306 (1), 208-221 (2007)
   PUBMED   17442300
REFERENCE   6  (bases 1 to 9024)
  AUTHORS   Garcia-Garcia,M.J., Eggenschwiler,J.T., Caspary,T., Alcorn,H.L.,
            Wyler,M.R., Huangfu,D., Rakeman,A.S., Lee,J.D., Feinberg,E.H.,
            Timmer,J.R. and Anderson,K.V.
  TITLE     Analysis of mouse embryonic patterning and morphogenesis by forward
            genetics
  JOURNAL   Proc Natl Acad Sci U S A 102 (17), 5913-5919 (2005)
   PUBMED   15755804
REFERENCE   7  (bases 1 to 9024)
  AUTHORS   Kasarskis,A., Manova,K. and Anderson,K.V.
  TITLE     A phenotype-based screen for embryonic lethal mutations in the
            mouse
  JOURNAL   Proc Natl Acad Sci U S A 95 (13), 7485-7490 (1998)
   PUBMED   9636176
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000006.1.
            
            On Apr 19, 2023 this sequence version replaced XM_006240086.3.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR26643287.15942.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA5760383,
                                           SAMEA5760386 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-373               JAXUCZ010000006.1  75003036-75003408   c
            374-535             JAXUCZ010000006.1  75001392-75001553   c
            536-770             JAXUCZ010000006.1  74978214-74978448   c
            771-998             JAXUCZ010000006.1  74975609-74975836   c
            999-1280            JAXUCZ010000006.1  74974216-74974497   c
            1281-1551           JAXUCZ010000006.1  74973843-74974113   c
            1552-1628           JAXUCZ010000006.1  74972551-74972627   c
            1629-1727           JAXUCZ010000006.1  74972371-74972469   c
            1728-1842           JAXUCZ010000006.1  74970246-74970360   c
            1843-2017           JAXUCZ010000006.1  74969425-74969599   c
            2018-2156           JAXUCZ010000006.1  74966352-74966490   c
            2157-2327           JAXUCZ010000006.1  74966052-74966222   c
            2328-2550           JAXUCZ010000006.1  74962981-74963203   c
            2551-2814           JAXUCZ010000006.1  74962115-74962378   c
            2815-2891           JAXUCZ010000006.1  74961928-74962004   c
            2892-3026           JAXUCZ010000006.1  74959025-74959159   c
            3027-3173           JAXUCZ010000006.1  74957476-74957622   c
            3174-3268           JAXUCZ010000006.1  74955931-74956025   c
            3269-3374           JAXUCZ010000006.1  74954162-74954267   c
            3375-3509           JAXUCZ010000006.1  74948749-74948883   c
            3510-3649           JAXUCZ010000006.1  74947532-74947671   c
            3650-3973           JAXUCZ010000006.1  74946918-74947241   c
            3974-4140           JAXUCZ010000006.1  74945144-74945310   c
            4141-4317           JAXUCZ010000006.1  74944878-74945054   c
            4318-5154           JAXUCZ010000006.1  74940712-74941548   c
            5155-5376           JAXUCZ010000006.1  74939872-74940093   c
            5377-5510           JAXUCZ010000006.1  74936318-74936451   c
            5511-5633           JAXUCZ010000006.1  74935747-74935869   c
            5634-5790           JAXUCZ010000006.1  74934131-74934287   c
            5791-6013           JAXUCZ010000006.1  74932279-74932501   c
            6014-6068           JAXUCZ010000006.1  74930005-74930059   c
            6069-6251           JAXUCZ010000006.1  74927898-74928080   c
            6252-6459           JAXUCZ010000006.1  74927254-74927461   c
            6460-6545           JAXUCZ010000006.1  74927084-74927169   c
            6546-6673           JAXUCZ010000006.1  74926786-74926913   c
            6674-6875           JAXUCZ010000006.1  74924740-74924941   c
            6876-7013           JAXUCZ010000006.1  74923188-74923325   c
            7014-7621           JAXUCZ010000006.1  74921574-74922181   c
            7622-7700           JAXUCZ010000006.1  74919753-74919831   c
            7701-7794           JAXUCZ010000006.1  74919578-74919671   c
            7795-7945           JAXUCZ010000006.1  74918895-74919045   c
            7946-8090           JAXUCZ010000006.1  74917845-74917989   c
            8091-9024           JAXUCZ010000006.1  74916803-74917736   c
FEATURES             Location/Qualifiers
     source          1..9024
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="6"
                     /map="6q23"
     gene            1..9024
                     /gene="Hectd1"
                     /gene_synonym="RGD1561653"
                     /note="HECT domain E3 ubiquitin protein ligase 1"
                     /db_xref="GeneID:362736"
                     /db_xref="RGD:1561653"
     exon            1..373
                     /gene="Hectd1"
                     /gene_synonym="RGD1561653"
                     /inference="alignment:Splign:2.1.0"
     exon            374..535
                     /gene="Hectd1"
                     /gene_synonym="RGD1561653"
                     /inference="alignment:Splign:2.1.0"
     CDS             396..8228
                     /gene="Hectd1"
                     /gene_synonym="RGD1561653"
                     /EC_number="2.3.2.26"
                     /note="isoform 1 is encoded by transcript variant 1; HECT
                     domain containing 1; HECT domain containing E3 ubiquitin
                     protein ligase 1; E3 ubiquitin-protein ligase HECTD1"
                     /codon_start=1
                     /product="E3 ubiquitin-protein ligase HECTD1 isoform 1"
                     /protein_id="NP_001406767.1"
                     /db_xref="GeneID:362736"
                     /db_xref="RGD:1561653"
                     /translation="MADVDPDTLLEWLQMGQGDERDMQLIALEQLCMLLLMSDNVDRC
                     FETCPPRTFLPALCKIFLDESAPDNVLEVTARAITYYLDVSAECTRRIVGVDGAIKAL
                     CNRLVVVELNNRTSRDLAEQCVKVLELICTRESGAVFEAGGLNCVLTFIRDSGHLVHK
                     DTLHSAMAVVSRLCGKMEPQDSSLEICVESLSSLLKHEDHQVSDGALRCFASLADRFT
                     RRGVDPAPLAKHGLTEELLSRMAAAGGTVSGPSSACKPGRSTTGAPSAAADSKLSNQV
                     STIVSLLSTLCRGSPLVTHDLLRSELPDSIESALQGDERCVLDTMRLVDLLLVLLFEG
                     RKALPKSSAGSTGRIPGLRRLDSSGERSHRQLIDCIRSKDTDALIDAIDTGAFEVNFM
                     DDVGQTLLNWASAFGTQEMVEFLCERGADVNRGQRSSSLHYAACFGRPQVAKTLLRHG
                     ANPDLRDEDGKTPLDKARERGHSEVVAILQSPGDWMCPVNKGDDKKKKDTNKDEEECN
                     EPKGDPEMAPVYLKRLLPVFAQTFQHTMLPSIRKASLALIRKMIHFCSEALLKEVCDS
                     DAGHNLPTALVEITATVLDQEDDDDGHLLALQIIRDLVDKGGDIFLDQLARLGVISKV
                     SALAGPSSDDENEEESKPEKEDEPQEDAKELQQGKPYHWRDWSVIRGRDCLYIWSDAA
                     ALELSNGSNGWFRFILDGKLATMYSSGSPEGGSDSSESRSEFLEKLQRARGQVKPSTS
                     SQPILSAPGPTKLTVGNWSLTCLKEGEIAIHNSDGQQATILKEDLPGFVFESNRGTKH
                     SFTAETSLGSEFVTGWTGKRGRKLKSKLEKTKQKVRTMARDLYDDHFKAVESMPRGVV
                     VTLRNIATQLESSWELHTNRQCIEGENTWRDLMKTALENLIVLLKDESTISPYEMCSS
                     GLVQALLTVLNSSIDLDMKQDCSQLVERINVFKTAFSESEDDESRPAVALIRKLIAVL
                     ESIERLPLHLYDTPGSTYNLQILTRRLRFRLERAPGETALIDRTGRMLKMEPLATVES
                     LEQYLLKMVAKQWYDFDRSSFVFVRKLREGQNFIFRHQHDFDENGIIYWIGTNAKTAY
                     EWVNPAAYGLVVVTSSEGRNLPYGRLEDILSRDNSALNCHSNDDKNAWFAIDLGVWVI
                     PSAYTLRHARGYGRSALRNWVFQVSKDGQNWTSLYTHVDDCSLNEPGSTATWPLDPAK
                     DEKQGWRHVRLKQMGKNASGQTHYLSLSGFELYGTVNGVCEDQLGKAAKEAEANLRRQ
                     RRLVRSQVLKYMVPGARVIRGLDWKWRDQDGSPQGEGTVTGELHNGWIDVTWDAGGSN
                     SYRMGAEGKFDLKLAPGYDPDTVASPKPVSSTVSGTTQSWSSLVKNNCPDKTSAAAGS
                     SSRKGSSSSVCSVASSSDISLGSTKTERRSEIVMEHSIVSGADVHEPIVVLSSAENVP
                     QTEVGSSSSASTSTLTAETGSENAERKLGPDSSVRAPGESSAISMGIVSVSSPDVSSV
                     SELTNKEAASQRPLSSSASNRLSVSSLLAAGAPMSSSASVPNLSSRETSSLESFVRRV
                     ANIARTNATNNMNLSRSSSDNNTNTLGRNVMSTATSPLMGAQSFPNLTTPGTTSTVTM
                     STSSVTSSSNVATATTVLSVGQSLSNTLTTSLTSTSSESDTGQEAEYSLYDFLDSCRA
                     STLLAELDDDEDLPEPDEEDDENEDDNQEDQEYEEVMILRRPSLQRRAGSRSDVTHHA
                     VTSQLPQVPSGAGSRPIGEQEEEEYETKGGRRRTWDDDYVLKRQFSALVPAFDPRPGR
                     TNVQQTTDLEIPPPGTPHSELLEEVECTPSPRLALTLKVTGLGTTREVELPLSNFRST
                     IFYYVQKLLQLSCNGNVKSDKLRRIWEPTYTIMYREMKDSDKEKENGKMGCWSIEHVE
                     QYLGTDELPKNDLITYLQKNADAAFLRHWKLTGTNKSIRKNRNCSQLIAAYKDFCEHG
                     TRSGLNQGASSSLQSSDILNLTKEQPQAKAGNGQNPCGVEDVLQLLRILYIVASDPYS
                     RISQEDGDEQPQFTFPPDEFTSKKITTKILQQIEEPLALASGALPDWCEQLTSKCPFL
                     IPFETRQLYFTCTAFGASRAIVWLQNRREATVERTRTTSSVRRDDPGEFRVGRLKHER
                     VKVPRGESLMEWAENVMQIHADRKSVLEVEFLGEEGTGLGPTLEFYALVAAEFQRTDL
                     GTWLCDDNFPDDESRHVDLGGGLKPPGYYVQRSCGLFTAPFPQDSDELERITKLFHFL
                     GIFLAKCIQDNRLVDLPISKPFFKLMCMGDIKSNMSKLIYESRGDRDLHCTESQSEAS
                     TEEGHDSLSVGSFEEDSKSEFILDPPKPKPPAWFNGILTWEDFELVNPHRARFLKEIK
                     DLAIKRRQILGNKSLSEDEKNTKLQELVLKNPSGSGPPLSIEDLGLNFQFCPSSRIYG
                     FTAVDLKPSGEDEMITMDNAEEYVDLMFDFCMHTGIQKQMEAFRDGFNKVFPMEKLSS
                     FSHEEVQMILCGNQSPSWAAEDIINYTEPKLGYTRDSPGFLRFVRVLCGMSSDERKAF
                     LQFTTGCSTLPPGGLANLHPRLTVVRKVDATDASYPSVNTCVHYLKLPEYSSEEIMRE
                     RLLAATMEKGFHLN"
     exon            536..770
                     /gene="Hectd1"
                     /gene_synonym="RGD1561653"
                     /inference="alignment:Splign:2.1.0"
     exon            771..998
                     /gene="Hectd1"
                     /gene_synonym="RGD1561653"
                     /inference="alignment:Splign:2.1.0"
     exon            999..1280
                     /gene="Hectd1"
                     /gene_synonym="RGD1561653"
                     /inference="alignment:Splign:2.1.0"
     exon            1281..1551
                     /gene="Hectd1"
                     /gene_synonym="RGD1561653"
                     /inference="alignment:Splign:2.1.0"
     exon            1552..1628
                     /gene="Hectd1"
                     /gene_synonym="RGD1561653"
                     /inference="alignment:Splign:2.1.0"
     exon            1629..1727
                     /gene="Hectd1"
                     /gene_synonym="RGD1561653"
                     /inference="alignment:Splign:2.1.0"
     exon            1728..1842
                     /gene="Hectd1"
                     /gene_synonym="RGD1561653"
                     /inference="alignment:Splign:2.1.0"
     exon            1843..2017
                     /gene="Hectd1"
                     /gene_synonym="RGD1561653"
                     /inference="alignment:Splign:2.1.0"
     exon            2018..2156
                     /gene="Hectd1"
                     /gene_synonym="RGD1561653"
                     /inference="alignment:Splign:2.1.0"
     exon            2157..2327
                     /gene="Hectd1"
                     /gene_synonym="RGD1561653"
                     /inference="alignment:Splign:2.1.0"
     exon            2328..2550
                     /gene="Hectd1"
                     /gene_synonym="RGD1561653"
                     /inference="alignment:Splign:2.1.0"
     exon            2551..2814
                     /gene="Hectd1"
                     /gene_synonym="RGD1561653"
                     /inference="alignment:Splign:2.1.0"
     exon            2815..2891
                     /gene="Hectd1"
                     /gene_synonym="RGD1561653"
                     /inference="alignment:Splign:2.1.0"
     exon            2892..3026
                     /gene="Hectd1"
                     /gene_synonym="RGD1561653"
                     /inference="alignment:Splign:2.1.0"
     exon            3027..3173
                     /gene="Hectd1"
                     /gene_synonym="RGD1561653"
                     /inference="alignment:Splign:2.1.0"
     exon            3174..3268
                     /gene="Hectd1"
                     /gene_synonym="RGD1561653"
                     /inference="alignment:Splign:2.1.0"
     exon            3269..3374
                     /gene="Hectd1"
                     /gene_synonym="RGD1561653"
                     /inference="alignment:Splign:2.1.0"
     exon            3375..3509
                     /gene="Hectd1"
                     /gene_synonym="RGD1561653"
                     /inference="alignment:Splign:2.1.0"
     exon            3510..3649
                     /gene="Hectd1"
                     /gene_synonym="RGD1561653"
                     /inference="alignment:Splign:2.1.0"
     exon            3650..3973
                     /gene="Hectd1"
                     /gene_synonym="RGD1561653"
                     /inference="alignment:Splign:2.1.0"
     exon            3974..4140
                     /gene="Hectd1"
                     /gene_synonym="RGD1561653"
                     /inference="alignment:Splign:2.1.0"
     exon            4141..4317
                     /gene="Hectd1"
                     /gene_synonym="RGD1561653"
                     /inference="alignment:Splign:2.1.0"
     exon            4318..5154
                     /gene="Hectd1"
                     /gene_synonym="RGD1561653"
                     /inference="alignment:Splign:2.1.0"
     exon            5155..5376
                     /gene="Hectd1"
                     /gene_synonym="RGD1561653"
                     /inference="alignment:Splign:2.1.0"
     exon            5377..5510
                     /gene="Hectd1"
                     /gene_synonym="RGD1561653"
                     /inference="alignment:Splign:2.1.0"
     exon            5511..5633
                     /gene="Hectd1"
                     /gene_synonym="RGD1561653"
                     /inference="alignment:Splign:2.1.0"
     exon            5634..5790
                     /gene="Hectd1"
                     /gene_synonym="RGD1561653"
                     /inference="alignment:Splign:2.1.0"
     exon            5791..6013
                     /gene="Hectd1"
                     /gene_synonym="RGD1561653"
                     /inference="alignment:Splign:2.1.0"
     exon            6014..6068
                     /gene="Hectd1"
                     /gene_synonym="RGD1561653"
                     /inference="alignment:Splign:2.1.0"
     exon            6069..6251
                     /gene="Hectd1"
                     /gene_synonym="RGD1561653"
                     /inference="alignment:Splign:2.1.0"
     exon            6252..6459
                     /gene="Hectd1"
                     /gene_synonym="RGD1561653"
                     /inference="alignment:Splign:2.1.0"
     exon            6460..6545
                     /gene="Hectd1"
                     /gene_synonym="RGD1561653"
                     /inference="alignment:Splign:2.1.0"
     exon            6546..6673
                     /gene="Hectd1"
                     /gene_synonym="RGD1561653"
                     /inference="alignment:Splign:2.1.0"
     exon            6674..6875
                     /gene="Hectd1"
                     /gene_synonym="RGD1561653"
                     /inference="alignment:Splign:2.1.0"
     exon            6876..7013
                     /gene="Hectd1"
                     /gene_synonym="RGD1561653"
                     /inference="alignment:Splign:2.1.0"
     exon            7014..7621
                     /gene="Hectd1"
                     /gene_synonym="RGD1561653"
                     /inference="alignment:Splign:2.1.0"
     exon            7622..7700
                     /gene="Hectd1"
                     /gene_synonym="RGD1561653"
                     /inference="alignment:Splign:2.1.0"
     exon            7701..7794
                     /gene="Hectd1"
                     /gene_synonym="RGD1561653"
                     /inference="alignment:Splign:2.1.0"
     exon            7795..7945
                     /gene="Hectd1"
                     /gene_synonym="RGD1561653"
                     /inference="alignment:Splign:2.1.0"
     exon            7946..8090
                     /gene="Hectd1"
                     /gene_synonym="RGD1561653"
                     /inference="alignment:Splign:2.1.0"
     exon            8091..9024
                     /gene="Hectd1"
                     /gene_synonym="RGD1561653"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 cttcaccagc ctccgcgccc ggcgctcggc cgacccttct cctgtccccg cccggctggc
       61 gatggacgcg gcctctcctc gctgccggcg gcagctccgc gcggacctgg ctttgaaccg
      121 cagggtctga ggagccgcgt gctggggtcg ggacccgcgc tggctgccgc cgctcggggg
      181 aacctctcgc ttccttgtca ccgggtcccc gcggaggaag gggagggacg agacacggct
      241 tttcctgtgc cgcctgcctg gctcgacctg cgcggcctgc ggtttcctgg gaactcgagg
      301 gccgcggccg ggccaggctc tgcccgcggc ctgcttggag ctggaccgga gctgtttggt
      361 acggcgagcc agggcaaagg gacattttaa agaccatggc agatgtggat cctgatacgt
      421 tgctggagtg gctgcagatg ggacagggag atgaaaggga catgcagcta atagccctgg
      481 aacagctatg catgttgctt ttgatgtctg acaatgtgga tcggtgtttt gaaacatgtc
      541 cacctcgtac tttcctacca gctctctgca aaatttttct tgatgaaagt gctccagaca
      601 atgtcttaga ggtgacagct cgtgccataa cgtactacct ggatgtgtct gcagagtgca
      661 cccgaaggat tgtaggggtg gatggggcta taaaagcact ctgtaatcgc ttggtggtgg
      721 ttgaactcaa caacagaacc agcagagact tagctgaaca gtgtgtaaag gtgctggaac
      781 tgatatgcac tcgtgagtca ggagcagtct ttgaggctgg tggtttgaat tgtgttctca
      841 ccttcattcg tgacagcgga cacctagttc ataaagatac attacactct gcgatggcgg
      901 tggtatcgag actctgtggt aaaatggaac ctcaggattc ttctctagaa atttgtgtag
      961 agtctctatc tagtttattg aaacatgaag atcatcaggt ttcagacgga gctctgaggt
     1021 gttttgcatc actagccgac cgatttactc gtcgaggagt ggacccagct ccattggcca
     1081 agcacggctt aactgaggag ctgttatctc gaatggcagc tgctggtggc actgtatcag
     1141 gaccgtcttc agcatgcaaa ccgggtcgca gtaccacagg agccccctct gcagctgcag
     1201 attccaaatt gagtaaccag gtgtcaacaa ttgtaagtct tctctccaca ctttgcagag
     1261 gctctccact agtaacacac gatcttctga ggtcagagct tccagattca attgaaagtg
     1321 cattacaggg tgatgaaaga tgtgtgcttg ataccatgcg tctggttgac ctcctcttgg
     1381 tgctgttatt tgaaggacga aaggctttgc ctaagtctag tgctggatct acaggcagaa
     1441 tcccaggact acggagactg gatagttctg gggaacgctc ccatcggcag cttatagact
     1501 gtattcgaag taaagatact gatgctctca tagatgcaat tgacacagga gcctttgaag
     1561 taaattttat ggatgatgtg ggtcagactc tattaaattg ggcctctgcg tttggaactc
     1621 aggaaatggt agaatttctg tgtgagaggg gcgctgacgt taatagaggc caaaggtcct
     1681 catcgttaca ctatgctgca tgttttggaa gacctcaagt agcaaagact ctgttacggc
     1741 atggtgcaaa tccggatctc agagatgaag atgggaaaac accattggat aaagctcgag
     1801 aaagaggcca cagtgaagta gtagcaattc tccagtcgcc aggtgattgg atgtgtccag
     1861 ttaataaagg ggatgataaa aaaaagaaag atacaaacaa agatgaagaa gagtgtaatg
     1921 agcccaaagg agacccggaa atggcaccag tatacttgaa gaggctgttg cctgtgtttg
     1981 cacaaacatt tcagcacact atgttgcctt caataaggaa agccagtctt gctttaattc
     2041 gaaaaatgat tcacttttgc tctgaagcac tgttaaaaga agtctgtgat tctgacgctg
     2101 gtcacaattt gcccacagca ctagtggaaa tcactgcgac tgtccttgac caagaggatg
     2161 atgatgatgg ccacttacta gctttgcaga tcataaggga cttagtagat aaaggtggtg
     2221 atattttttt ggatcagctc gccagacttg gtgtaattag caaagtgtca gctttggcag
     2281 gtccttcttc tgatgatgag aatgaagagg aatcaaaacc agaaaaggaa gacgaaccac
     2341 aggaagacgc taaagaattg cagcaaggca agccgtacca ttggagagac tggtcagtaa
     2401 tcagaggacg ggactgctta tatatctgga gtgatgctgc agctttggag ttgtctaatg
     2461 gcagtaatgg atggttcaga tttatcttgg atggaaaact tgccaccatg tattcaagtg
     2521 gtagtcctga aggtggatcg gatagttcag aaagcagaag tgaattctta gagaagttac
     2581 aaagagcccg tggccaagta aagccatcta cttcaagtca acctatcctg tcagcaccgg
     2641 ggcccactaa acttaccgta gggaactggt cattaacatg tttgaaagaa ggagaaattg
     2701 ctattcataa ctcagatggt cagcaagcta caatactaaa ggaagatttg cctggctttg
     2761 tatttgaatc taatagagga actaagcatt cgtttactgc agaaacatct ctgggttcag
     2821 agtttgtgac tggctggact ggcaaaagag gcaggaaatt aaaatccaaa ttggaaaaaa
     2881 caaagcaaaa ggtacgaact atggcccgag atttgtatga tgaccacttt aaagctgtgg
     2941 aaagcatgcc tcgtggagtg gtggtaacac tcagaaatat agcaactcag ttggagtcat
     3001 cttgggaact tcatacgaat agacaatgca tcgaaggtga aaacacatgg cgagacttaa
     3061 tgaagacagc attagaaaac ctgattgtgc ttctgaagga tgagagcacg atctccccat
     3121 atgagatgtg cagcagtggc ttagtccaag ccctgctcac tgtattgaac agtagtatag
     3181 atttggatat gaagcaagat tgcagtcaac tggtagaaag aataaatgtc ttcaaaacag
     3241 ccttcagtga aagtgaagat gatgaaagta gaccagcagt tgcattaatt cgaaagttaa
     3301 tagctgtact agaatcaatt gagcgactac ctctccattt gtatgataca ccaggatcca
     3361 catataacct ccagatactg acaagaagat tacgctttcg gttggaacgt gcacctggtg
     3421 aaactgcatt aatagaccgg acaggcagga tgctgaagat ggagccctta gctacagttg
     3481 aatctctgga acagtatctg ctgaaaatgg tagcaaagca gtggtacgat tttgaccggt
     3541 cctcatttgt ttttgttcga aaattgagag aaggccaaaa ttttatattt cggcaccagc
     3601 atgattttga tgaaaatgga atcatttact ggattggaac aaatgcaaaa actgcatatg
     3661 aatgggtaaa tcctgcagct tatggtcttg tagtcgtgac atcatcagaa ggaagaaatc
     3721 taccatatgg ccgcttggaa gacatactaa gtcgagataa ttcagcttta aattgtcata
     3781 gcaatgatga taaaaatgcc tggtttgcca tagatctggg tgtctgggta ataccatcag
     3841 catacacact tcgtcatgct cgtggctatg gaaggtctgc actgagaaac tgggttttcc
     3901 aggtatccaa agatggacag aactggactt ctttgtatac ccatgttgat gactgcagtc
     3961 tcaatgaacc agggtcaact gcaacgtggc cacttgaccc agcaaaggat gaaaaacaag
     4021 gatggcggca tgtgaggctg aagcagatgg gaaagaacgc aagcggacag acacactacc
     4081 tctccctctc tggctttgag ctctatggca ctgtaaatgg cgtgtgtgaa gaccaactcg
     4141 ggaaagcagc taaagaagca gaagctaatc ttagaaggca gaggcgcctg gtgcgttctc
     4201 aagtcctgaa gtacatggtt ccaggagcac gtgttatcag aggccttgac tggaagtggc
     4261 gagatcagga tggcagccca cagggagaag gcacagtcac aggagagctg cacaatggct
     4321 ggattgatgt cacctgggat gctggtggct caaactctta ccgtatgggc gcagaaggaa
     4381 aatttgacct caagcttgca ccagggtacg accctgatac agtggcatca cccaaacctg
     4441 tttcatccac tgtttcaggc acaacgcagt catggagcag cttggtgaaa aacaactgtc
     4501 cagacaagac gtctgctgct gcaggctcct caagtagaaa aggaagcagc agttctgtgt
     4561 gtagcgtggc cagtagcagc gacatcagct tgggttcgac caaaacggaa cggagatcag
     4621 aaattgtaat ggaacacagt atagtttcag gagctgatgt ccatgaacca attgttgttc
     4681 tttcatctgc tgaaaacgtt cctcaaacag aagtagggtc atcttccagt gcaagcacca
     4741 gcaccctaac agcggaaacg ggaagtgaaa atgctgaaag gaagttaggc ccggatagtt
     4801 ctgttcgagc tcctggggag tctagtgcaa tatccatggg aattgttagc gttagctctc
     4861 ctgatgttag ttcagtatct gaattaacta ataaggaagc agcttcacaa cgacccctta
     4921 gctcgtcagc aagtaacaga ctgtcagtga gctctctgtt ggcagctggg gcccctatga
     4981 gctctagtgc aagtgtacct aacctgtcct caagagaaac atctagcttg gagagctttg
     5041 taaggagagt ggcaaacata gcacggacta atgccaccaa caacatgaac ctgagccgga
     5101 gcagcagtga taacaacact aacactctgg ggcggaatgt gatgagcacc gcaacttctc
     5161 ctcttatggg tgctcagagt ttccctaatt tgaccacacc tggtactaca tcgacagtga
     5221 caatgtcaac atcaagtgtt actagcagca gcaatgtagc tacggcgaca acagttttat
     5281 cagtgggtca gtcattaagt aatactttaa ccaccagcct cacatcaacc tctagtgaga
     5341 gtgacacagg gcaggaagca gaatattcct tatatgactt tcttgatagc tgtcgtgcca
     5401 gtactctctt agctgagcta gatgatgacg aggacttgcc tgaaccggat gaggaagacg
     5461 atgagaacga agatgacaat caggaggacc aagaatacga ggaggttatg attctgagac
     5521 gtccatcttt gcaacgtcgt gctggctctc gctctgatgt aacacaccat gctgtcacct
     5581 cacagctacc acaggtccct tctggagcag ggagccgacc tattggggaa caggaagaag
     5641 aagagtatga aactaaagga ggccgccgga gaacctggga tgacgactac gtgctgaagc
     5701 ggcagttctc tgcactggtt cccgcttttg atcctaggcc tggtcgtacc aatgtccaac
     5761 agacaactga cctagaaatc cctcccccag gaacacctca ctcagagctc ttggaggaag
     5821 tcgagtgtac tccatcacct cgattggctc tcacgttgaa agtgactggg cttggaacga
     5881 ctcgggaagt tgaactgcca cttagcaact tcagatcaac catcttttat tatgtacaga
     5941 aactgcttca gctgtcttgt aatggcaatg tgaagtcaga caaacttagg cgtatttggg
     6001 agccaactta cacaatcatg tatagagaaa tgaaggattc tgataaagaa aaggaaaatg
     6061 ggaaaatggg ttgctggtct atagagcatg tggaacagta ccttggcact gatgaattac
     6121 caaagaatga cttgataacc tacctgcaga agaatgctga cgccgctttc cttcgccact
     6181 ggaaattaac tggcactaat aaaagtatta ggaaaaacag aaattgttct cagcttatag
     6241 ccgcatataa ggatttttgt gagcatggaa caaggtctgg attaaaccag ggggctagtt
     6301 ctagtcttca aagcagtgat attcttaatt tgacaaaaga gcagcctcag gctaaagccg
     6361 gcaacggaca gaacccctgt ggagtagagg acgttcttca gcttctgcgc attctgtata
     6421 tagttgcaag tgacccttat tcaaggatat cccaagaaga tggtgatgag cagccccagt
     6481 ttacttttcc acctgatgaa tttactagta aaaagatcac cacaaaaatt ttgcagcaga
     6541 ttgaggagcc attggctttg gcaagcgggg ctctgccaga ctggtgtgaa cagctgacca
     6601 gcaagtgtcc tttcctaatc ccattcgaaa ccagacagct ttacttcacc tgtactgcgt
     6661 ttggtgcatc cagagcaata gtgtggctac agaatcgacg ggaagccaca gtggagcgaa
     6721 caaggaccac gagcagtgta aggcgagatg atcctgggga atttagagtt ggccgactca
     6781 agcacgaaag agtgaaggtt ccccgtggag agtctctgat ggagtgggct gagaatgtca
     6841 tgcaaataca cgcagaccgg aagtcagttc tcgaggttga atttctagga gaagaaggaa
     6901 ctggcctggg acccacttta gagttttatg ctcttgtggc agcagaattc cagaggaccg
     6961 acttggggac ttggctttgt gatgataact ttccagatga tgaatctcgt catgttgacc
     7021 ttggaggtgg gttgaagcct cctggatact atgtacagcg atcatgtgga ctgttcacag
     7081 cgccatttcc acaagacagt gatgagctgg aaaggatcac aaaactcttc catttccttg
     7141 ggattttctt ggccaaatgt attcaagaca atagattggt ggacttgcct atttctaagc
     7201 ctttctttaa acttatgtgc atgggcgaca ttaaaagcaa tatgagcaaa ctgatctatg
     7261 aatcacgagg cgacagagac ttgcactgca ctgaaagtca gtctgaagcg tctacggaag
     7321 aaggtcatga ctccctctca gtaggaagct ttgaagagga ttcaaaatca gaatttatcc
     7381 ttgatccccc taagcccaaa cccccggctt ggtttaatgg aattttaact tgggaagatt
     7441 ttgaactagt aaatccacac agagccagat ttttaaaaga aattaaagac cttgctatta
     7501 agaggcgcca gattttaggc aataaaagcc tttccgaaga tgagaagaac acaaagttac
     7561 aggaactagt actcaagaac ccatcaggtt ctggacctcc acttagcata gaggacttag
     7621 gcttaaattt ccagttttgc ccatcttcca gaatatatgg cttcacagcg gtggacctca
     7681 agccaagtgg agaagatgag atgataacaa tggataatgc agaagaatat gtggatttga
     7741 tgtttgactt ttgtatgcat acgggtatcc agaaacagat ggaagctttt cgagatgggt
     7801 ttaataaagt ttttccaatg gagaaattaa gttccttcag ccatgaggaa gtccagatga
     7861 ttctttgtgg aaatcaatca ccatcctggg cagcagagga cattatcaat tatactgaac
     7921 ctaagcttgg ctatacacgt gacagtcccg gcttccttag gtttgtgagg gtcttatgtg
     7981 gcatgtcctc agacgaacgg aaagcattcc tgcagtttac caccggctgt tcaactctgc
     8041 ccccaggtgg actggctaac ctgcatccga ggctcacagt cgtgcgaaag gttgatgcta
     8101 ctgatgcaag ctatccatcc gtcaacactt gtgtccacta tcttaagtta cctgaatatt
     8161 cttctgagga gatcatgaga gagcgcttgc tagctgctac aatggagaaa ggctttcatc
     8221 tcaactgagt tttgaaatgc agaagagaga cagaagtccg ggtgaaggct cttgcgtttg
     8281 tttgcagaga agtgtatgat tctccacagt aatgacactt gccttgttct ccatcattaa
     8341 ggctttgaga agacgtggga taaattcctt agctgctaat gacaaaatac atcctttaac
     8401 tacccagcca gcaggtatcc agcacaggac actgtgttgc tttacaaggg ctcatgtgac
     8461 tggaaaaggt ggttctactt aactgttcca cagagcagcc tctcagatct tcagtgctca
     8521 ctgtaaatgt aaccgtgttt gtatcaagtt tgtcatcgtt tcatacttca tacactacag
     8581 ttgccatcac tgacccccgt tttgctggct ttttagctac tcggtcaaaa atactgcttc
     8641 cttaaaacat agtgttaatg agcatctcag cttttctttt cctctttatg acatctgcac
     8701 tgccagtgtc cagtgctctc tctttgttcg gcatagaatc atgcacaccc ttgtcatttc
     8761 tttaaggtgg gagtatattt ttttttcata aataccattc tacaaagatg aaattctaaa
     8821 atgtgtttgt gcagctcaaa aataaagata tattaaaaga ggggcaaaca ctggtctagg
     8881 tgcaaggcac actcacagca atgtttatgt ttggttgtat tttctttgta tattataaac
     8941 atttatttaa cttgttgcag tttgaagtaa atttctgaaa tgtatgctca acaataatca
     9001 ttaaaatgtt tgcagcgtag gaaa
//
