LOCUS       NM_021658               3947 bp    mRNA    linear   ROD 27-MAR-2024
DEFINITION  Rattus norvegicus hyperpolarization activated cyclic
            nucleotide-gated potassium channel 4 (Hcn4), mRNA.
ACCESSION   NM_021658
VERSION     NM_021658.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 3947)
  AUTHORS   Sun,X., Jin,K., Ding,X., Ruan,Z. and Xu,P.
  TITLE     DNA methylation cooperates with H3K9me2 at HCN4 promoter to
            regulate the differentiation of bone marrow mesenchymal stem cells
            into pacemaker-like cells
  JOURNAL   PLoS One 18 (8), e0289510 (2023)
   PUBMED   37643180
  REMARK    GeneRIF: DNA methylation cooperates with H3K9me2 at HCN4 promoter
            to regulate the differentiation of bone marrow mesenchymal stem
            cells into pacemaker-like cells.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3947)
  AUTHORS   Parveen,S., Cheah,P.H.S., Worthington,L.P.I., Smither,R.A.,
            Munro,M.L., Bussey,C.T., Lamberts,R.R. and Jones,P.P.
  TITLE     Depressed HCN4 function in the type 2 diabetic sinoatrial node
  JOURNAL   Mol Cell Biochem 478 (8), 1825-1833 (2023)
   PUBMED   36574099
  REMARK    GeneRIF: Depressed HCN4 function in the type 2 diabetic sinoatrial
            node.
REFERENCE   3  (bases 1 to 3947)
  AUTHORS   Zhengshuai,L., Yan,F., Jinglin,L., Hua,P. and Chunmei,W.
  TITLE     Glucocorticoid Receptor/HCN4 Channels Interaction in Spinal Dorsal
            Horn Participates in the Regulation of Neuropathic Pain after
            Peripheral Nerve Injury in Rats
  JOURNAL   Bull Exp Biol Med 173 (5), 594-601 (2022)
   PUBMED   36214984
  REMARK    GeneRIF: Glucocorticoid Receptor/HCN4 Channels Interaction in
            Spinal Dorsal Horn Participates in the Regulation of Neuropathic
            Pain after Peripheral Nerve Injury in Rats.
REFERENCE   4  (bases 1 to 3947)
  AUTHORS   Logantha,S.J.R.J., Kharche,S.R., Zhang,Y., Atkinson,A.J., Hao,G.,
            Boyett,M.R. and Dobrzynski,H.
  TITLE     Sinus node-like pacemaker mechanisms regulate ectopic pacemaker
            activity in the adult rat atrioventricular ring
  JOURNAL   Sci Rep 9 (1), 11781 (2019)
   PUBMED   31409881
  REMARK    GeneRIF: Sinus node-like pacemaker mechanisms regulate ectopic
            pacemaker activity in the adult rat atrioventricular ring.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 3947)
  AUTHORS   Zuniga,R., Gonzalez,D., Valenzuela,C., Brown,N. and Zuniga,L.
  TITLE     Expression and cellular localization of HCN channels in rat
            cerebellar granule neurons
  JOURNAL   Biochem Biophys Res Commun 478 (3), 1429-1435 (2016)
   PUBMED   27569278
  REMARK    GeneRIF: the expression and relative abundances of HCN channels in
            cerebellum and primary cultures of cerebellar granule neurons, is
            described.
REFERENCE   6  (bases 1 to 3947)
  AUTHORS   Schulze-Bahr,E., Neu,A., Friederich,P., Kaupp,U.B., Breithardt,G.,
            Pongs,O. and Isbrandt,D.
  TITLE     Pacemaker channel dysfunction in a patient with sinus node disease
  JOURNAL   J Clin Invest 111 (10), 1537-1545 (2003)
   PUBMED   12750403
REFERENCE   7  (bases 1 to 3947)
  AUTHORS   Stevens,D.R., Seifert,R., Bufe,B., Muller,F., Kremmer,E., Gauss,R.,
            Meyerhof,W., Kaupp,U.B. and Lindemann,B.
  TITLE     Hyperpolarization-activated channels HCN1 and HCN4 mediate
            responses to sour stimuli
  JOURNAL   Nature 413 (6856), 631-635 (2001)
   PUBMED   11675786
REFERENCE   8  (bases 1 to 3947)
  AUTHORS   Monteggia,L.M., Eisch,A.J., Tang,M.D., Kaczmarek,L.K. and
            Nestler,E.J.
  TITLE     Cloning and localization of the hyperpolarization-activated cyclic
            nucleotide-gated channel family in rat brain
  JOURNAL   Brain Res Mol Brain Res 81 (1-2), 129-139 (2000)
   PUBMED   11000485
REFERENCE   9  (bases 1 to 3947)
  AUTHORS   Shi,W., Wymore,R., Yu,H., Wu,J., Wymore,R.T., Pan,Z.,
            Robinson,R.B., Dixon,J.E., McKinnon,D. and Cohen,I.S.
  TITLE     Distribution and prevalence of hyperpolarization-activated cation
            channel (HCN) mRNA expression in cardiac tissues
  JOURNAL   Circ Res 85 (1), e1-e6 (1999)
   PUBMED   10400919
REFERENCE   10 (bases 1 to 3947)
  AUTHORS   Ludwig,A., Zong,X., Stieber,J., Hullin,R., Hofmann,F. and Biel,M.
  TITLE     Two pacemaker channels from human heart with profoundly different
            activation kinetics
  JOURNAL   EMBO J 18 (9), 2323-2329 (1999)
   PUBMED   10228147
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from
            JAXUCZ010000008.1.
            
            On Nov 27, 2020 this sequence version replaced NM_021658.1.
            
            Sequence Note: This sequence has been modified as follows: removed
            22 bp suspected to be vector contamination from the 3' end.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF247453.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760393, SAMEA5760433
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1010              JAXUCZ010000008.1  68118062-68119071
            1011-1434           JAXUCZ010000008.1  68138702-68139125
            1435-1596           JAXUCZ010000008.1  68148213-68148374
            1597-1815           JAXUCZ010000008.1  68150387-68150605
            1816-1962           JAXUCZ010000008.1  68152340-68152486
            1963-2203           JAXUCZ010000008.1  68152595-68152835
            2204-2368           JAXUCZ010000008.1  68153633-68153797
            2369-3947           JAXUCZ010000008.1  68153904-68155482
FEATURES             Location/Qualifiers
     source          1..3947
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="8"
                     /map="8q24"
     gene            1..3947
                     /gene="Hcn4"
                     /note="hyperpolarization activated cyclic nucleotide-gated
                     potassium channel 4"
                     /db_xref="GeneID:59266"
                     /db_xref="RGD:71065"
     exon            1..1010
                     /gene="Hcn4"
                     /inference="alignment:Splign:2.1.0"
     CDS             226..3822
                     /gene="Hcn4"
                     /note="hyperpolarization-activated, cyclic
                     nucleotide-gated K+ 4"
                     /codon_start=1
                     /product="potassium/sodium hyperpolarization-activated
                     cyclic nucleotide-gated channel 4"
                     /protein_id="NP_067690.1"
                     /db_xref="GeneID:59266"
                     /db_xref="RGD:71065"
                     /translation="MDKLPPSMRKRLYSLPQQVGAKAWIMDEEEDGEEEGAGGLQDPS
                     RRSIRLRPLPSPSPSVAAGCSESRGAALGAADSEGPGRSAGKSSTNGDCRRFRGSLAS
                     LGSRGGGSGGAGGGSSLGHLHDSAEERRLIAAEGDASPGEDRTPPGLATEPERPGAAA
                     QPAASPPPQQPPQPASASCEQPSADTAIKVEGGAAASDQILPEAEVRLGQSGFMQRQF
                     GAMLQPGVNKFSLRMFGSQKAVEREQERVKSAGFWIIHPYSDFRFYWDLTMLLLMVGN
                     LIIIPVGITFFKDENTTPWIVFNVVSDTFFLIDLVLNFRTGIVVEDNTEIILDPQRIK
                     MKYLKSWFVVDFISSIPVDYIFLIVETRIDSEVYKTARALRIVRFTKILSLLRLLRLS
                     RLIRYIHQWEEIFHMTYDLASAVVRIVNLIGMMLLLCHWDGCLQFLVPMLQDFPHDCW
                     VSINGMVNNSWGKQYSYALFKAMSHMLCIGYGRQAPVGMSDVWLTMLSMIVGATCYAM
                     FIGHATALIQSLDSSRRQYQEKYKQVEQYMSFHKLPPDTRQRIHDYYEHRYQGKMFDE
                     ESILGELSEPLREEIINFNCRKLVASMPLFANADPNFVTSMLTKLRFEVFQPGDYIIR
                     EGTIGKKMYFIQHGVVSVLTKGNKETKLADGSYFGEICLLTRGRRTASVRADTYCRLY
                     SLSVDNFNEVLEEYPMMRRAFETVALDRLDRIGKKNSILLHKVQHDLNSGVFNYQENE
                     IIQQIVRHDREMAHCAHRVQAAASATPTPTPVIWTPLIQAPLQAAAATTSVAIALTHH
                     PRLPAAIFRPPPGPGLGNLGAGQTPRHPRRLQSLIPSALGSASPASSPSQVDTPSSSS
                     FHIQQLAGFSAPPGLSPLLPSSSSSPPPGACSSPPAPTPSTSTAATTTGFGHFHKALG
                     GSLSSSDSPLLTPLQPGARSPQAAQPPPPLPGARGGLGLLEHFLPPPPSSRSPSSSPG
                     QLGQPPGELSPGLAAGPPSTPETPPRPERPSFMAGASGGASPVAFTPRGGLSPPGHSP
                     GPPRTFPSAPPRASGSHGSLLLPPASSPPPPQVPQRRGTPPLTPGRLTQDLKLISASQ
                     PALPQDGAQTLRRASPHSSGESMAAFSLYPRAGGGSGSSGGLGPPGRPYGAIPGQHVT
                     LPRKTSSGSLPPPLSLFGARAASSGGPPLTAAPQREPGARSEPVRSKLPSNL"
     exon            1011..1434
                     /gene="Hcn4"
                     /inference="alignment:Splign:2.1.0"
     exon            1435..1596
                     /gene="Hcn4"
                     /inference="alignment:Splign:2.1.0"
     exon            1597..1815
                     /gene="Hcn4"
                     /inference="alignment:Splign:2.1.0"
     exon            1816..1962
                     /gene="Hcn4"
                     /inference="alignment:Splign:2.1.0"
     exon            1963..2203
                     /gene="Hcn4"
                     /inference="alignment:Splign:2.1.0"
     exon            2204..2368
                     /gene="Hcn4"
                     /inference="alignment:Splign:2.1.0"
     exon            2369..3947
                     /gene="Hcn4"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gagggaggga acgtggggtc cccgcgccca agcccggccc gggaggaggt gcagcgcggc
       61 gaacccgggg acccgcagcc ggactaggag cctcccctcg gcgcgcagcc ggcgagatca
      121 tgggctcgtg aggcagcgaa ggacgagtcc tccctggcag gcgcacctgg agcgggtccc
      181 ccgtgccgca cccgcaccgc tgcccccggc ccagtccctg gcaccatgga caagctgccg
      241 ccgtccatgc gcaagcggct ctacagcctt ccgcagcagg tgggggccaa ggcgtggatc
      301 atggacgagg aagaggatgg tgaggaagag ggggccgggg gcctccagga cccaagccga
      361 aggagcattc ggctgcggcc gctgccctcg ccctcgccct cggtggccgc cggctgctcc
      421 gagtcccggg gtgcggccct cggggcggca gacagcgagg ggccgggccg cagcgccggc
      481 aagtccagca ccaacggtga ctgcaggcgc ttccgcggga gtctggcctc gctgggcagc
      541 cggggcggcg gcagtggtgg agcagggggc ggcagcagtc tcgggcacct gcatgactcc
      601 gcggaggagc ggcggctcat cgccgctgag ggcgatgcgt cccccggcga ggacaggacg
      661 cccccgggcc tggcgaccga gcccgagcgc ccgggcgccg cggcacaacc cgcagcctcg
      721 ccgccgcccc aacagccgcc gcagccggcc tccgcctcct gcgagcagcc ctcggcggac
      781 acagctatca aagtggaagg aggcgcggcc gccagcgacc agatcctccc tgaggccgag
      841 gtgcgcctgg gccagagcgg cttcatgcag cgccagttcg gtgccatgct gcaacctggg
      901 gtcaacaaat tctcgctaag gatgttcggc agccagaaag cagtggagcg tgagcaggag
      961 agggttaagt cagctgggtt ttggattatc cacccctaca gcgacttcag attttactgg
     1021 gacctgacga tgctgttgct gatggtgggg aatctgatca tcatccctgt gggcatcacc
     1081 ttcttcaagg atgagaacac caccccctgg atcgtcttca acgtggtgtc agacacattc
     1141 ttcctcattg acttggtcct caacttccgc acggggatcg tggtggagga caacacagaa
     1201 atcatccttg acccacagcg gatcaagatg aagtacctga aaagctggtt tgtggtggac
     1261 ttcatctcct ccatccccgt ggactacatc ttccttatag tggagactcg cattgactcg
     1321 gaggtctaca aaaccgccag ggctctgcgc attgtccgct tcacgaagat cctcagcctc
     1381 ctgcgcctcc tgcggctttc ccgcctcatt cggtacattc accagtggga agagatcttc
     1441 cacatgacct acgacctggc cagtgccgtg gtacgcatcg tgaacctcat tggcatgatg
     1501 cttctgcttt gccactggga tggctgcctg cagttcctgg tgcccatgct gcaggacttc
     1561 ccccatgact gctgggtgtc catcaacggc atggtgaata actcctgggg gaagcagtac
     1621 tcctatgccc tcttcaaggc catgagccac atgctgtgta ttgggtacgg acggcaggca
     1681 cccgtaggca tgtctgacgt ctggctcacc atgctcagca tgatcgtggg cgccacctgc
     1741 tatgccatgt tcatcgggca cgccactgcc ctcatccagt cgctggactc ctcccggcgc
     1801 cagtaccagg agaagtacaa gcaggtggag cagtacatgt ccttccacaa gctcccgcct
     1861 gacaccaggc agcgcatcca cgactactac gaacaccgct accagggcaa gatgtttgac
     1921 gaggaaagca tcctgggtga gctgagtgag ccgcttcgag aggagatcat caactttaac
     1981 tgccggaagc tggtggcatc catgccactg ttcgccaatg cagaccccaa ctttgtgacg
     2041 tctatgctga ccaagttgcg ttttgaggtc tttcagcctg gggactacat catccgtgaa
     2101 ggcaccatcg gcaagaagat gtactttatc cagcacggcg tggtcagtgt gctcactaag
     2161 ggcaacaagg agaccaagct ggctgatggc tcctattttg gagagatctg cttgctgacc
     2221 cgaggccgtc gcacagcgag cgtgagggcg gatacttact gccgcctcta ctcactgagc
     2281 gtggacaact tcaacgaggt gctggaggag tatcccatga tgcgcagggc tttcgagacg
     2341 gttgcgctgg accgtctgga ccgcataggc aagaagaact ccatcctcct ccacaaggtg
     2401 cagcacgacc tcaactcagg cgtcttcaac taccaagaga acgagatcat ccagcagatc
     2461 gtgcggcatg accgtgagat ggcccactgt gctcaccgcg tccaggctgc tgcctcagcc
     2521 accccaaccc caacgcctgt catatggacc ccactgatcc aggcaccact gcaggctgct
     2581 gctgctacta cttcggtggc catagccctc acacaccacc cccgcctgcc agccgctatc
     2641 ttccggcccc ctcccggacc tgggctgggt aacctggggg ctggacagac accgaggcac
     2701 ccaaggaggt tgcagtcctt gatcccttca gcgctaggct ctgcttcacc agccagcagc
     2761 ccctcacagg tggacacacc gtcttcatct tccttccaca tccaacagct ggctggattc
     2821 tctgcacctc ctggattgag tcctctcttg ccctcctcta gctcttcccc acctccagga
     2881 gcctgcagtt ctcccccagc ccccactcca tccacctcca ctgctgccac caccaccggg
     2941 ttcggccact ttcataaggc gctaggtggc tccctgtctt cctctgattc cccgctgctc
     3001 accccactgc aaccgggcgc tcgctctcca caggctgccc agccgccacc cccactgcct
     3061 ggggcccgag gaggcctggg actcctggag cacttcttgc cgcccccacc ttcgtcccgg
     3121 tcaccatcat ctagccctgg gcagctgggc cagcctcctg gagagttgtc cccaggtctg
     3181 gcagctggtc caccaagtac accagagaca cccccgcggc ccgaacggcc atcctttatg
     3241 gcaggggcct ctgggggggc ttctcctgta gcctttaccc cccgaggagg cctcagccct
     3301 ccgggccaca gcccaggacc cccaagaact ttcccgagtg ccccaccccg ggcctctggc
     3361 tcccatggtt ccctgctcct gccacctgca tccagccctc cgcctcccca ggtcccacag
     3421 cgcaggggca caccacccct cacccccggc cgcctcacac aggacctgaa gctcatctca
     3481 gcctctcagc cagccctccc ccaggatggg gcacagactc tacgcagggc ctctcctcac
     3541 tcctcagggg agtcgatggc tgccttctca ctctacccca gagctggggg tggcagtggg
     3601 agcagtgggg gccttgggcc tcctggaagg ccatatggtg ccatcccagg ccagcatgtc
     3661 actttgcctc ggaagacatc ctcaggttct ttgccacccc cactttcttt gtttggggca
     3721 agagccgcct cttctggagg gccccctctg actgctgcac cccagaggga acctggcgct
     3781 aggtccgagc cagtacgctc caaactgccg tctaatttat gagctgggcc ctccctcctt
     3841 ccctcttctt tttcttgctc ccttcttcct tcaggtttaa ctgtgattag gagatatacc
     3901 aataacaata ataaccataa aaaacatacc ccagaaaaac aaaaaga
//
