LOCUS       NM_053685               3296 bp    mRNA    linear   ROD 31-MAR-2024
DEFINITION  Rattus norvegicus hyperpolarization-activated cyclic
            nucleotide-gated potassium channel 3 (Hcn3), mRNA.
ACCESSION   NM_053685
VERSION     NM_053685.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 3296)
  AUTHORS   Li,D., Liu,H., Liu,X., Wang,H., Li,T., Wang,X., Jia,S., Wang,P. and
            Wang,Y.F.
  TITLE     Involvement of Hyperpolarization-Activated Cyclic Nucleotide-Gated
            Channel 3 in Oxytocin Neuronal Activity in Lactating Rats With Pup
            Deprivation
  JOURNAL   ASN Neuro 12, 1759091420944658 (2020)
   PUBMED   32962418
  REMARK    GeneRIF: Involvement of Hyperpolarization-Activated Cyclic
            Nucleotide-Gated Channel 3 in Oxytocin Neuronal Activity in
            Lactating Rats With Pup Deprivation.
REFERENCE   2  (bases 1 to 3296)
  AUTHORS   Negrini,D., Marcozzi,C., Solari,E., Bossi,E., Cinquetti,R.,
            Reguzzoni,M. and Moriondo,A.
  TITLE     Hyperpolarization-activated cyclic nucleotide-gated channels in
            peripheral diaphragmatic lymphatics
  JOURNAL   Am J Physiol Heart Circ Physiol 311 (4), H892-H903 (2016)
   PUBMED   27496876
  REMARK    GeneRIF: Data suggest that HCN channels might play an important
            role in affecting contraction frequency of lymphatic muscle cells.
REFERENCE   3  (bases 1 to 3296)
  AUTHORS   Zuniga,R., Gonzalez,D., Valenzuela,C., Brown,N. and Zuniga,L.
  TITLE     Expression and cellular localization of HCN channels in rat
            cerebellar granule neurons
  JOURNAL   Biochem Biophys Res Commun 478 (3), 1429-1435 (2016)
   PUBMED   27569278
  REMARK    GeneRIF: the expression and relative abundances of HCN channels in
            cerebellum and primary cultures of cerebellar granule neurons, is
            described.
REFERENCE   4  (bases 1 to 3296)
  AUTHORS   Huang,X., Yang,P., Yang,Z., Zhang,H. and Ma,A.
  TITLE     Age-associated expression of HCN channel isoforms in rat sinoatrial
            node
  JOURNAL   Exp Biol Med (Maywood) 241 (3), 331-339 (2016)
   PUBMED   26341471
  REMARK    GeneRIF: During the senescent process, the HCN2 and HCN4 protein
            levels declined.
REFERENCE   5  (bases 1 to 3296)
  AUTHORS   Cao-Ehlker,X., Zong,X., Hammelmann,V., Gruner,C., Fenske,S.,
            Michalakis,S., Wahl-Schott,C. and Biel,M.
  TITLE     Up-regulation of hyperpolarization-activated cyclic
            nucleotide-gated channel 3 (HCN3) by specific interaction with K+
            channel tetramerization domain-containing protein 3 (KCTD3)
  JOURNAL   J Biol Chem 288 (11), 7580-7589 (2013)
   PUBMED   23382386
REFERENCE   6  (bases 1 to 3296)
  AUTHORS   Mistrik,P., Mader,R., Michalakis,S., Weidinger,M., Pfeifer,A. and
            Biel,M.
  TITLE     The murine HCN3 gene encodes a hyperpolarization-activated cation
            channel with slow kinetics and unique response to cyclic
            nucleotides
  JOURNAL   J Biol Chem 280 (29), 27056-27061 (2005)
   PUBMED   15923185
REFERENCE   7  (bases 1 to 3296)
  AUTHORS   Notomi,T. and Shigemoto,R.
  TITLE     Immunohistochemical localization of Ih channel subunits, HCN1-4, in
            the rat brain
  JOURNAL   J Comp Neurol 471 (3), 241-276 (2004)
   PUBMED   14991560
  REMARK    GeneRIF: Immunoreactivity for HCN3 in rat brain shows subcortical
            distribution mainly concentrated in the hypothalamus.
REFERENCE   8  (bases 1 to 3296)
  AUTHORS   Muller,F., Scholten,A., Ivanova,E., Haverkamp,S., Kremmer,E. and
            Kaupp,U.B.
  TITLE     HCN channels are expressed differentially in retinal bipolar cells
            and concentrated at synaptic terminals
  JOURNAL   Eur J Neurosci 17 (10), 2084-2096 (2003)
   PUBMED   12786975
  REMARK    GeneRIF: densely clustered at synaptic terminals of retinal bipolar
            cells and photoreceptors.
REFERENCE   9  (bases 1 to 3296)
  AUTHORS   Monteggia,L.M., Eisch,A.J., Tang,M.D., Kaczmarek,L.K. and
            Nestler,E.J.
  TITLE     Cloning and localization of the hyperpolarization-activated cyclic
            nucleotide-gated channel family in rat brain
  JOURNAL   Brain Res Mol Brain Res 81 (1-2), 129-139 (2000)
   PUBMED   11000485
REFERENCE   10 (bases 1 to 3296)
  AUTHORS   Shi,W., Wymore,R., Yu,H., Wu,J., Wymore,R.T., Pan,Z.,
            Robinson,R.B., Dixon,J.E., McKinnon,D. and Cohen,I.S.
  TITLE     Distribution and prevalence of hyperpolarization-activated cation
            channel (HCN) mRNA expression in cardiac tissues
  JOURNAL   Circ Res 85 (1), e1-e6 (1999)
   PUBMED   10400919
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000002.1.
            
            On Oct 3, 2022 this sequence version replaced NM_053685.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF247452.1, SRR26643298.11960.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA5760383,
                                           SAMEA5760389 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-409               JAXUCZ010000002.1  176863245-176863653 c
            410-839             JAXUCZ010000002.1  176856052-176856481 c
            840-1001            JAXUCZ010000002.1  176854522-176854683 c
            1002-1220           JAXUCZ010000002.1  176853905-176854123 c
            1221-1367           JAXUCZ010000002.1  176853467-176853613 c
            1368-1608           JAXUCZ010000002.1  176852864-176853104 c
            1609-1773           JAXUCZ010000002.1  176851546-176851710 c
            1774-3296           JAXUCZ010000002.1  176849635-176851157 c
FEATURES             Location/Qualifiers
     source          1..3296
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="2"
                     /map="2q34"
     gene            1..3296
                     /gene="Hcn3"
                     /note="hyperpolarization-activated cyclic nucleotide-gated
                     potassium channel 3"
                     /db_xref="GeneID:114245"
                     /db_xref="RGD:620691"
     exon            1..409
                     /gene="Hcn3"
                     /inference="alignment:Splign:2.1.0"
     CDS             135..2477
                     /gene="Hcn3"
                     /codon_start=1
                     /product="potassium/sodium hyperpolarization-activated
                     cyclic nucleotide-gated channel 3"
                     /protein_id="NP_446137.1"
                     /db_xref="GeneID:114245"
                     /db_xref="RGD:620691"
                     /translation="MEEEARPAVGDGEAATPARETPPAAPAQARAASGGVPESAPEPK
                     RRQLGTLLQPTVNKFSLRVFGSHKAVEIEQERVKSAGAWIIHPYSDFRFYWDLIMLLL
                     MVGNLIVLPVGITFFKEENSPPWIVFNVLSDTFFLLDLVLNFRTGIVVEEGAEILLAP
                     RAIRTRYLRTWFLVDLISSIPVDYIFLVVELEPRLDAEVYKTARALRIVRFTKILSLL
                     RLLRLSRLIRYMHQWEEIFHMTYDLASAVVRIFNLIGMMLLLCHWDGCLQFLVPMLQD
                     FPSDCWVSMNRMVNHSWGRQYSHALFKAMSHMLCIGYGQQAPVGMPDVWLTMLSMIVG
                     ATCYAMFIGHATALIQSLDSSRRQYQEKYKQVEQYMSFHKLPADTRQRIHEYYEHRYQ
                     GKMFDEESILGELSEPLREEIINFTCRGLVAHMPLFAHADPSFVTAVLTKLRFEVFQP
                     GDLVVREGSVGRKMYFIQHGLLSVLARGARDTRLTDGSYFGEICLLTRGRRTASVRAD
                     TYCRLYSLSVDHFNAVLEEFPMMRRAFETVAMDRLRRIGKKNSILQRKRSEPSPGSSS
                     GGVMEQHLVQHDRDMARGIRGLAPGTGARLSGKPVLWEPLVHAPLQAAAVTSNVAIAL
                     THQRGPLPLSPDSPATLLARSARRSAGSPASPLVPVRAGPLLARGPWASTSRLPAPPA
                     RTLHASLSRTGRSQVSLLGPPPGGGGRRLGPRGRPLSASQPSLPQRATGDGSPRRKGS
                     GSERLPPSGLLAKPPGTVQPSRSSVPEPVTPRGPQISANM"
     exon            410..839
                     /gene="Hcn3"
                     /inference="alignment:Splign:2.1.0"
     exon            840..1001
                     /gene="Hcn3"
                     /inference="alignment:Splign:2.1.0"
     exon            1002..1220
                     /gene="Hcn3"
                     /inference="alignment:Splign:2.1.0"
     exon            1221..1367
                     /gene="Hcn3"
                     /inference="alignment:Splign:2.1.0"
     exon            1368..1608
                     /gene="Hcn3"
                     /inference="alignment:Splign:2.1.0"
     exon            1609..1773
                     /gene="Hcn3"
                     /inference="alignment:Splign:2.1.0"
     exon            1774..3296
                     /gene="Hcn3"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gtccccctgc acacgccccc tgcgcccggg gcgcacgcgg gtcggagctc ccctgcctgg
       61 gctgcgctcc caggcacctg cgcgcccgcc acggccatgg accggttgga gaggcccaac
      121 gagtgagggc agccatggag gaggaggcgc ggccggcggt gggggacggg gaagcggcga
      181 ctcctgcacg cgagacgcct cctgcggctc ccgcccaggc ccgcgcggcc tcaggtgggg
      241 tgccagagtc tgcgcccgag ccgaagaggc ggcagctcgg gacgctgctg cagccgaccg
      301 tcaacaagtt ctctctccgg gtcttcggca gccacaaagc ggtggaaatc gagcaggaga
      361 gggtgaagtc cgccggggcc tggatcatcc acccctacag cgacttccgg ttttactggg
      421 acctgatcat gctgctgctg atggtgggga acctcatagt actgcccgtg ggcatcactt
      481 tcttcaagga ggagaactcc ccaccctgga tcgtcttcaa tgtcctctcg gacactttct
      541 tcctgctgga tctggtgctc aacttccgaa ctggcatcgt ggtggaggaa ggtgcggaga
      601 tcctgttggc gcccagggct atccgcacgc gttacctgcg cacctggttc ctggtggacc
      661 tgatttcctc catccctgtg gattacatct tcctagtggt agagctggag ccacgactag
      721 acgctgaggt ctacaaaacg gcacgggccc tgcgcatcgt tagattcacc aagatcctta
      781 gcctgctgcg gctgctccgc ctctcccgcc tcatccgata catgcaccag tgggaggaga
      841 tctttcacat gacctacgac ctggccagtg cagtggttcg catcttcaac ctcattggaa
      901 tgatgttgct gctgtgtcac tgggatggct gtctgcagtt cctggtccct atgctgcagg
      961 acttcccttc cgactgctgg gtctccatga accgcatggt gaaccactcg tggggccgcc
     1021 agtactccca cgccctgttc aaggccatga gtcacatgct gtgcattggc tacgggcagc
     1081 aggcaccagt aggcatgcct gacgtctggc tcaccatgct cagcatgatt gtgggcgcca
     1141 cctgctatgc catgttcatc ggccacgcca ccgccctcat ccagtccctg gactcgtccc
     1201 ggcgccagta ccaggagaag tacaagcagg tggagcagta catgtccttc cacaagctgc
     1261 cagccgacac acggcagcgc atccacgagt actacgagca tcggtaccag ggcaagatgt
     1321 tcgatgaaga gagcatcctg ggggagctga gcgagccgct tcgggaggag attattaact
     1381 tcacctgccg gggcctggtg gcccacatgc cgctgtttgc tcatgctgac cccagtttcg
     1441 tcaccgcagt actcaccaag ctccgttttg aggtcttcca acctggggat ctggtggtgc
     1501 gtgagggctc cgtgggcagg aagatgtact tcatccagca tgggctgctc agtgtgttgg
     1561 cacggggcgc ccgggacact cgcctcactg acggatccta ctttggggag atctgcctgc
     1621 tgactcgagg tcggagaaca gccagtgtaa gggctgacac ctactgtcgc ctctactcac
     1681 tcagcgtgga ccacttcaat gcagtgcttg aggagttccc gatgatgcgc agggcttttg
     1741 agactgtggc catggaccgg cttcggcgca tcggcaaaaa gaattcgata ttgcagcgga
     1801 aacgctctga gccgagtcca ggcagcagca gtggtggcgt catggagcag catttggtac
     1861 aacacgacag agacatggct cgtggtattc ggggtctggc tccgggcaca ggagctcgcc
     1921 tcagtggaaa gccagtgctg tgggaaccac tggtacacgc acctctgcag gcagctgctg
     1981 tgacctccaa cgtggccata gccttgactc atcagcgagg ccctctgccc ctctcccctg
     2041 attctccagc caccctcctg gctcgatctg ctagacgctc agcaggctcc ccagcctccc
     2101 cactggtgcc tgtccgagca ggtcctctgc tggcccgggg accctgggcg tccacttctc
     2161 gcctgcctgc tccacctgcc cgaaccctcc atgccagcct atcccggaca gggcgttccc
     2221 aggtgtctct gttgggccct cccccaggag gaggtggtcg gaggctagga cctcggggcc
     2281 gcccactctc tgcctctcaa ccctctctgc ctcagcgagc cacgggggat ggctctccta
     2341 ggcgcaaagg ctctggaagt gagcgtctgc ccccctcggg gctcctggcc aagcctccag
     2401 ggactgtcca gccatccagg tcatcagtgc ctgagccagt tacccccaga ggtccccaaa
     2461 tttctgccaa catgtgaaat cctagattgc actggcctta gctattggca cgcaataagg
     2521 tgtgcctgag ggtagacgct ccctggggaa ggctgcgggg atctaacaca ctgcccttta
     2581 ctaccacaaa gttgtcctct gtgtcctcag ctcatagcag ctgcagcctg cgaatcctaa
     2641 tcacataccc atttgtcata ggtaccagtg cctaccccgt caaggcattg tgctaagtac
     2701 aatgtgtagc cactgccaag tagaagtgac tcaaagccct ttaacaaggg tgtccccagg
     2761 ccacagtcct gccaggtgca ggcttgggcc cacagccctg cctttattaa gcacaagtac
     2821 ttgctgcttc cttcactgcc gagtgactag atggctgtct ccctgtcagt gaccctgcag
     2881 gttgtgccca gtgtgtcctt tcttcccatc cccattgtcg tagctgggta ctgtcaatta
     2941 tctagtacag ccaacagatg tctagggccc taggtgtgaa catcttccct ctcactgcca
     3001 cgtgtggacg gaccgtcctt gtctacacca cgtgagaacg ggtatctctg cccactgtag
     3061 tctgccctcc gcagctcctg tgtggaaagc actacctttg taaactagaa gctgctcttc
     3121 actgtgctcc ctgactcaat ggtgttgccc taaagctaag ggatacttgg cgcctcctta
     3181 ctgtgctagc ctagctcccc ccaggtaggg ggtaaggggc tgaatcctca tgtgaccttt
     3241 tatctttccc tgtgtcttat tcagtcactt attaatttta tttattcatt tgctaa
//
