LOCUS       NM_053684               3200 bp    mRNA    linear   ROD 27-JUL-2024
DEFINITION  Rattus norvegicus hyperpolarization activated cyclic nucleotide
            gated potassium and sodium channel 2 (Hcn2), mRNA.
ACCESSION   NM_053684 XM_343170
VERSION     NM_053684.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 3200)
  AUTHORS   Zeng,X., Mai,J., Xie,H., Yang,L. and Liu,X.
  TITLE     Activation of CB1R alleviates central sensitization by regulating
            HCN2-pNR2B signaling in a chronic migraine rat model
  JOURNAL   J Headache Pain 24 (1), 44 (2023)
   PUBMED   37085778
  REMARK    GeneRIF: Activation of CB1R alleviates central sensitization by
            regulating HCN2-pNR2B signaling in a chronic migraine rat model.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3200)
  AUTHORS   Lei,X., Yan,Y., Zeng,J., Wang,R., Li,S., Xiao,Z. and Liu,X.
  TITLE     Activation of HCN channels caused by elevated cAMP levels in
            periaqueductal gray promotes bone cancer pain
  JOURNAL   Neurochem Int 162, 105437 (2023)
   PUBMED   36336089
  REMARK    GeneRIF: Activation of HCN channels caused by elevated cAMP levels
            in periaqueductal gray promotes bone cancer pain.
REFERENCE   3  (bases 1 to 3200)
  AUTHORS   Singh,N., Zabbarova,I., Ikeda,Y., Kanai,A., Chermansky,C.,
            Yoshimura,N. and Tyagi,P.
  TITLE     Role of hyperpolarization-activated cyclic nucleotide-gated
            channels in aging bladder phenotype
  JOURNAL   Life Sci 289, 120203 (2022)
   PUBMED   34875252
  REMARK    GeneRIF: Role of hyperpolarization-activated cyclic
            nucleotide-gated channels in aging bladder phenotype.
REFERENCE   4  (bases 1 to 3200)
  AUTHORS   Yavuz,M., Aydin,B., Carcak,N. and Onat,F.
  TITLE     Decreased Hyperpolarization-Activated Cyclic Nucleotide-Gated
            Channel 2 Activity in a Rat Model of Absence Epilepsy and the
            Effect of ZD7288, an Ih Inhibitor, on the Spike-and-Wave Discharges
  JOURNAL   Pharmacology 107 (3-4), 227-234 (2022)
   PUBMED   35008085
  REMARK    GeneRIF: Decreased Hyperpolarization-Activated Cyclic
            Nucleotide-Gated Channel 2 Activity in a Rat Model of Absence
            Epilepsy and the Effect of ZD7288, an Ih Inhibitor, on the
            Spike-and-Wave Discharges.
REFERENCE   5  (bases 1 to 3200)
  AUTHORS   Hadova,K., Kralova,E., Doka,G., Bies Pivackova,L., Kmecova,Z.,
            Krenek,P. and Klimas,J.
  TITLE     Isolated downregulation of HCN2 in ventricles of rats with
            streptozotocin-induced diabetic cardiomyopathy
  JOURNAL   BMC Cardiovasc Disord 21 (1), 118 (2021)
   PUBMED   33653265
  REMARK    GeneRIF: Isolated downregulation of HCN2 in ventricles of rats with
            streptozotocin-induced diabetic cardiomyopathy.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 3200)
  AUTHORS   Muller,F., Scholten,A., Ivanova,E., Haverkamp,S., Kremmer,E. and
            Kaupp,U.B.
  TITLE     HCN channels are expressed differentially in retinal bipolar cells
            and concentrated at synaptic terminals
  JOURNAL   Eur J Neurosci 17 (10), 2084-2096 (2003)
   PUBMED   12786975
  REMARK    GeneRIF: densely clustered at synaptic terminals of retinal bipolar
            cells and photoreceptors.
REFERENCE   7  (bases 1 to 3200)
  AUTHORS   Monteggia,L.M., Eisch,A.J., Tang,M.D., Kaczmarek,L.K. and
            Nestler,E.J.
  TITLE     Cloning and localization of the hyperpolarization-activated cyclic
            nucleotide-gated channel family in rat brain
  JOURNAL   Brain Res Mol Brain Res 81 (1-2), 129-139 (2000)
   PUBMED   11000485
REFERENCE   8  (bases 1 to 3200)
  AUTHORS   Pachucki,J., Burmeister,L.A. and Larsen,P.R.
  TITLE     Thyroid hormone regulates hyperpolarization-activated cyclic
            nucleotide-gated channel (HCN2) mRNA in the rat heart
  JOURNAL   Circ Res 85 (6), 498-503 (1999)
   PUBMED   10488052
REFERENCE   9  (bases 1 to 3200)
  AUTHORS   Shi,W., Wymore,R., Yu,H., Wu,J., Wymore,R.T., Pan,Z.,
            Robinson,R.B., Dixon,J.E., McKinnon,D. and Cohen,I.S.
  TITLE     Distribution and prevalence of hyperpolarization-activated cation
            channel (HCN) mRNA expression in cardiac tissues
  JOURNAL   Circ Res 85 (1), e1-e6 (1999)
   PUBMED   10400919
REFERENCE   10 (bases 1 to 3200)
  AUTHORS   Ludwig,A., Zong,X., Stieber,J., Hullin,R., Hofmann,F. and Biel,M.
  TITLE     Two pacemaker channels from human heart with profoundly different
            activation kinetics
  JOURNAL   EMBO J 18 (9), 2323-2329 (1999)
   PUBMED   10228147
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000007.1.
            
            On Mar 2, 2021 this sequence version replaced NM_053684.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AB164197.1, AF247451.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA5760383,
                                           SAMEA5760386 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-587               JAXUCZ010000007.1  10638871-10639457   c
            588-1011            JAXUCZ010000007.1  10631336-10631759   c
            1012-1173           JAXUCZ010000007.1  10629790-10629951   c
            1174-1392           JAXUCZ010000007.1  10627351-10627569   c
            1393-1539           JAXUCZ010000007.1  10625821-10625967   c
            1540-1780           JAXUCZ010000007.1  10622700-10622940   c
            1781-1945           JAXUCZ010000007.1  10622395-10622559   c
            1946-3200           JAXUCZ010000007.1  10620422-10621676   c
FEATURES             Location/Qualifiers
     source          1..3200
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="7"
                     /map="7q11"
     gene            1..3200
                     /gene="Hcn2"
                     /note="hyperpolarization activated cyclic nucleotide gated
                     potassium and sodium channel 2"
                     /db_xref="GeneID:114244"
                     /db_xref="RGD:620689"
     exon            1..587
                     /gene="Hcn2"
                     /inference="alignment:Splign:2.1.0"
     CDS             37..2628
                     /gene="Hcn2"
                     /note="hyperpolarization activated cyclic nucleotide-gated
                     potassium channel 2"
                     /codon_start=1
                     /product="potassium/sodium hyperpolarization-activated
                     cyclic nucleotide-gated channel 2"
                     /protein_id="NP_446136.1"
                     /db_xref="GeneID:114244"
                     /db_xref="RGD:620689"
                     /translation="MDARGGGGRPGDSPGATPAPGPPPPPPPPAPPQPQPPPAPPPNP
                     TTPSHPESADEPGPRSRLCSRDSSCTPGAAKGGANGECGRGEPQCSPEGPARGPKVSF
                     SCRGAASGPAAAEEAGSEEAGPAGEPRGSQASFLQRQFGALLQPGVNKFSLRMFGSQK
                     AVEREQERVKSAGAWIIHPYSDFRFYWDFTMLLFMVGNLIIIPVGITFFKDETTAPWI
                     VFNVVSDTFFLMDLVLNFRTGIVIEDNTEIILDPEKIKKKYLRTWFVVDFVSSIPVDY
                     IFLIVEKGIDSEVYKTARALRIVRFTKILSLLRLLRLSRLIRYIHQWEEIFHMTYDLA
                     SAVMRICNLISMMLLLCHWDGCLQFLVPMLQDFPSDCWVSINNMVNHSWSELYSFALF
                     KAMSHMLCIGYGRQAPESMTDIWLTMLSMIVGATCYAMFIGHATALIQSLDSSRRQYQ
                     EKYKQVEQYMSFHKLPADFRQKIHDYYEHRYQGKMFDEDSILGELNGPLREEIVNFNC
                     RKLVASMPLFANADPNFVTAMLTKLKFEVFQPGDYIIREGTIGKKMYFIQHGVVSVLT
                     KGNKEMKLSDGSYFGEICLLTRGRRTASVRADTYCRLYSLSVDNFNEVLEEYPMMRRA
                     FETVAIDRLDRIGKKNSILLHKVQHDLSSGVFNNQENAIIQEIVKYDREMVQQAELGQ
                     RVGLFPPPPPPQVTSAIATLQQAVAMSFCPQVARPLVGPLALGSPRLVRRAPPGPLPP
                     AASPGPPAASPPAAPSSPRAPRTSPYGVPGSPATRVGPALPARRLSRASRPLSASQPS
                     LPHGAPAPSPAASARPASSSTPRLGPAPTTRTAAPSPDRRDSASPGAASGLDPLDSAR
                     SRLSSNL"
     exon            588..1011
                     /gene="Hcn2"
                     /inference="alignment:Splign:2.1.0"
     exon            1012..1173
                     /gene="Hcn2"
                     /inference="alignment:Splign:2.1.0"
     exon            1174..1392
                     /gene="Hcn2"
                     /inference="alignment:Splign:2.1.0"
     exon            1393..1539
                     /gene="Hcn2"
                     /inference="alignment:Splign:2.1.0"
     exon            1540..1780
                     /gene="Hcn2"
                     /inference="alignment:Splign:2.1.0"
     exon            1781..1945
                     /gene="Hcn2"
                     /inference="alignment:Splign:2.1.0"
     exon            1946..3200
                     /gene="Hcn2"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 tccgctccgc tccgcactgc ccggcgccgc ctcgctatgg atgcgcgcgg gggcggcggg
       61 cggccgggcg atagtccggg cgcgacccct gcgccggggc cgccgccacc gccgccgccg
      121 cccgcgcccc ctcagcctca gccaccaccc gcgccacccc cgaaccccac gaccccctcg
      181 cacccggagt cggcggacga gcccggcccg cgctcccggc tctgcagccg cgacagctcc
      241 tgcactcctg gcgcggccaa gggcggcgcg aatggcgagt gcgggcgcgg ggagccgcag
      301 tgcagccccg agggccccgc gcgcggcccc aaggtttcgt tctcatgtcg cggggcggcc
      361 tcggggcccg cggcggccga ggaggcgggc agcgaggagg cgggcccggc gggtgagccg
      421 cgcggcagcc aggccagctt cctgcagcgc caattcgggg cgctcctgca gccgggcgtc
      481 aacaagttct ccctgcggat gtttggcagc cagaaggccg tggagcgcga gcaggaacgc
      541 gtgaagtcgg cgggggcctg gatcatccac ccctacagcg acttcaggtt ctactgggac
      601 ttcaccatgc tgttgttcat ggtgggaaat ctcatcatca tccctgtggg catcactttc
      661 ttcaaggacg agaccacggc gccctggatc gtcttcaacg tggtctcgga cactttcttc
      721 ctcatggact tggtgctgaa ctttcgcacc ggcattgtta ttgaggacaa cacggagatc
      781 atcctggacc ccgaaaagat aaagaaaaag tacctgcgta cgtggttcgt ggtagacttc
      841 gtgtcatcca tcccggtgga ctacatcttc ctcatcgtgg agaagggaat cgactccgag
      901 gtctacaaga cggcccgtgc actacgcatc gtgcgtttca ccaagatcct cagtctgctg
      961 cggttgctgc ggctatcccg gctcatccga tatatccacc aatgggagga gattttccac
     1021 atgacctacg acctggcaag cgcggtgatg cgcatctgta acctgatcag catgatgctg
     1081 ctgctctgcc actgggacgg ctgcctgcag ttcctggtgc ccatgctgca agacttcccc
     1141 agcgactgct gggtgtccat caacaacatg gtgaaccact cgtggagcga actctattcg
     1201 ttcgcgctct tcaaggccat gagccacatg ctctgtattg gctacgggcg gcaggctccc
     1261 gagagcatga cggacatctg gctcaccatg ctcagcatga tcgtgggcgc cacctgctac
     1321 gctatgttca ttgggcacgc cacggcgctt atccagtccc tggactcgtc acggcgccag
     1381 taccaggaga agtacaagca agtggagcag tacatgtcct tccacaaact gccggctgac
     1441 ttccgccaga agatccacga ttactatgaa caccggtacc aggggaagat gtttgacgag
     1501 gacagcatcc tgggggaact caacggccca ctgcgtgagg agattgtgaa cttcaactgc
     1561 cggaagctgg tggcttccat gccgttgttt gccaacgcag accccaactt cgtcaccgcc
     1621 atgctgacaa agctcaaatt tgaggtcttc cagcctggag actacatcat ccgagagggg
     1681 accatcggga agaagatgta cttcatccag cacggggtgg tgagcgtgct caccaagggc
     1741 aacaaggaga tgaagctgtc agatggctcc tattttgggg agatctgcct gctcacgagg
     1801 ggccggcgca cagccagtgt gcgggctgac acctactgtc gcctctactc actgagcgtg
     1861 gacaacttca acgaggtgct ggaggagtac cccatgatgc ggcgtgcctt tgagaccgtg
     1921 gccattgacc gcctggaccg cataggcaag aagaactcca tcttgctaca caaggttcag
     1981 catgatctca gctcgggtgt gttcaacaac caggagaacg ccatcatcca ggagattgtc
     2041 aaatatgacc gtgagatggt gcagcaggca gagctgggcc agcgtgtggg gctcttccca
     2101 ccaccgccac caccgcaggt cacgtcggcc atcgccacgc tgcagcaggc cgtggccatg
     2161 agcttctgcc cgcaggtggc ccgcccgctc gtggggcccc tggcgctagg gtccccacgc
     2221 ctcgtgcgcc gcgcgccccc agggcctctg cctcctgcag cctcaccagg gccacccgca
     2281 gcgagccccc cggctgcacc ctcgagccct cgggcaccgc ggacctcacc ctacggtgtg
     2341 cctggctctc cggcaacgcg tgtggggccc gcattgcccg cacgccgcct gagccgcgcc
     2401 tcgcgcccac tgtccgcctc gcagccctcg ctgccccacg gcgcgcccgc acccagcccc
     2461 gcggcctctg cgcgcccggc cagcagctcc accccgcgtc tgggacccgc acccaccacc
     2521 cggaccgcgg cacccagtcc ggaccgcagg gactcagcct cgccgggcgc tgccagtggc
     2581 ctcgacccac tggactctgc gcgctcgcgc ctctcttcca acttgtgacc cttgcgcgcc
     2641 gccccgcggg ccgggcgggg ccgtcatcca caccaaagcc atgcctcgcg ccgcccgccc
     2701 gtgcccgtgc agaagccata gagggacgta ggtagcttag gaggcgggcg gccctgcgcc
     2761 cggctgtccc cccattgccc tgcgcccacc ccatcgcccc tgccccagcg gcggccgcac
     2821 gggagaggga ggggtgcgat cacctcggtg cctcagcccc aacctgggac ggggacgggg
     2881 cggccctggc cgaggacctg gctgtgcccc gcatgtgcgg tggcctccga ggaagaatat
     2941 ggatcaagtg caatactcgg cccgccggct tcccgctgcc cccggcaagc tcacgcaata
     3001 accagctcgc ccctgcccac gcgtccgtgg tgacctccct cgggcaccca ggggcgggct
     3061 tcacggccaa gccggcgtgg gggtgaggct gggtccccgc cgtcgccatg aatgtactga
     3121 cgagccgagg cagcagtggc ccccacgccc cattaaccca caaccccatt ccgcgcaata
     3181 aacgacagca ttggcgccca
//
