LOCUS       NM_001014012            3089 bp    mRNA    linear   ROD 22-MAR-2023
DEFINITION  Rattus norvegicus histidyl-tRNA synthetase 2, mitochondrial
            (Hars2), mRNA; nuclear gene for mitochondrial product.
ACCESSION   NM_001014012 XM_226007
VERSION     NM_001014012.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 3089)
  AUTHORS   Baltz AG, Munschauer M, Schwanhausser B, Vasile A, Murakawa Y,
            Schueler M, Youngs N, Penfold-Brown D, Drew K, Milek M, Wyler E,
            Bonneau R, Selbach M, Dieterich C and Landthaler M.
  TITLE     The mRNA-bound proteome and its global occupancy profile on
            protein-coding transcripts
  JOURNAL   Mol Cell 46 (5), 674-690 (2012)
   PUBMED   22681889
REFERENCE   2  (bases 1 to 3089)
  AUTHORS   Pierce SB, Chisholm KM, Lynch ED, Lee MK, Walsh T, Opitz JM, Li W,
            Klevit RE and King MC.
  TITLE     Mutations in mitochondrial histidyl tRNA synthetase HARS2 cause
            ovarian dysgenesis and sensorineural hearing loss of Perrault
            syndrome
  JOURNAL   Proc Natl Acad Sci U S A 108 (16), 6543-6548 (2011)
   PUBMED   21464306
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            CK481952.1, CV075096.1 and BC089973.1.
            
            On Oct 1, 2008 this sequence version replaced NM_001014012.1.
            
            ##Evidence-Data-START##
            RNAseq introns :: single sample supports all introns SAMD00132261,
                              SAMD00132262 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: inferred from homology
            RefSeq Select criteria             :: based on conservation,
                                                  expression, longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-652               CK481952.1         17-668
            653-836             CV075096.1         256-439
            837-3089            BC089973.1         1-2253
FEATURES             Location/Qualifiers
     source          1..3089
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10116"
                     /chromosome="18"
                     /map="18p11"
     gene            1..3089
                     /gene="Hars2"
                     /gene_synonym="Hars2l; RGD1308426; Zmat2"
                     /note="histidyl-tRNA synthetase 2, mitochondrial"
                     /db_xref="GeneID:307491"
                     /db_xref="RGD:1308426"
     exon            1..224
                     /gene="Hars2"
                     /gene_synonym="Hars2l; RGD1308426; Zmat2"
                     /inference="alignment:Splign:2.1.0"
     CDS             117..1640
                     /gene="Hars2"
                     /gene_synonym="Hars2l; RGD1308426; Zmat2"
                     /EC_number="6.1.1.21"
                     /note="zinc finger, matrin type 2; histidyl-tRNA
                     synthetase 2, mitochondrial (putative)"
                     /codon_start=1
                     /product="probable histidine--tRNA ligase, mitochondrial"
                     /protein_id="NP_001014034.2"
                     /db_xref="GeneID:307491"
                     /db_xref="RGD:1308426"
                     /translation="MPHLGPLRCRTWAALLGQLLRPPRTVCNRAVGCHSQVAKAVLTS
                     EQLKPHQQKPNFIIKVPKGTRDLSPQQMVVREKILDKIISCFKRHGAKGLDTPAFELK
                     EMLTEKYEDNFGLMYDLEDQGGELLSLRYDLTVPFARYLAMNKLKRMKRYQVGKVWRR
                     ESPAIVQGRYREFCQCDFDIAGEFDPMIPDAECLRIMCEILSGLQLGDFLIKVNDRRV
                     VDGMFAVCGVPESKFRTICSSMDKLDKMSWEDVRLEMVAGKGLAPEVADRIGDYVQCH
                     GGISLVEELFKDPRLSQSQLALQGLGDLKLLFEYLRLFGIADKISLDLSLARGLDYYT
                     GVIYEAVLLESPAQAGKETLNVGSVAAGGRYDRLVAQFDPKGHSVPCVGLSIGVERIF
                     YLVEQKMKISCEKVRTTETQVFVATPQKNFLQERLKIIAQLWDAGIKAEMLYKNNPKL
                     LTQLHYCEKEDIPLMVIIGEQEQNEGVVKLRSVASREEVTVNRESLVAEIQKRLSES"
     exon            225..302
                     /gene="Hars2"
                     /gene_synonym="Hars2l; RGD1308426; Zmat2"
                     /inference="alignment:Splign:2.1.0"
     exon            303..422
                     /gene="Hars2"
                     /gene_synonym="Hars2l; RGD1308426; Zmat2"
                     /inference="alignment:Splign:2.1.0"
     exon            423..518
                     /gene="Hars2"
                     /gene_synonym="Hars2l; RGD1308426; Zmat2"
                     /inference="alignment:Splign:2.1.0"
     exon            519..644
                     /gene="Hars2"
                     /gene_synonym="Hars2l; RGD1308426; Zmat2"
                     /inference="alignment:Splign:2.1.0"
     exon            645..752
                     /gene="Hars2"
                     /gene_synonym="Hars2l; RGD1308426; Zmat2"
                     /inference="alignment:Splign:2.1.0"
     exon            753..851
                     /gene="Hars2"
                     /gene_synonym="Hars2l; RGD1308426; Zmat2"
                     /inference="alignment:Splign:2.1.0"
     exon            852..945
                     /gene="Hars2"
                     /gene_synonym="Hars2l; RGD1308426; Zmat2"
                     /inference="alignment:Splign:2.1.0"
     exon            946..1073
                     /gene="Hars2"
                     /gene_synonym="Hars2l; RGD1308426; Zmat2"
                     /inference="alignment:Splign:2.1.0"
     exon            1074..1316
                     /gene="Hars2"
                     /gene_synonym="Hars2l; RGD1308426; Zmat2"
                     /inference="alignment:Splign:2.1.0"
     exon            1317..1433
                     /gene="Hars2"
                     /gene_synonym="Hars2l; RGD1308426; Zmat2"
                     /inference="alignment:Splign:2.1.0"
     exon            1434..1580
                     /gene="Hars2"
                     /gene_synonym="Hars2l; RGD1308426; Zmat2"
                     /inference="alignment:Splign:2.1.0"
     exon            1581..3089
                     /gene="Hars2"
                     /gene_synonym="Hars2l; RGD1308426; Zmat2"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 ccggactaac tccgagctca ggcgtttcca gctctccgta gccttcctga aatcggactg
       61 gtctcattcc cagtcccttg acgtctgttc cagaaagccg gcgtcctgtt gcacaaatgc
      121 cccacctagg gcccctgcgc tgtaggacct gggctgcact actcggccaa ctcctgcgac
      181 ctccccgcac tgtgtgcaac cgggcggtcg gctgtcatag ccaggttgcg aaggcagtgt
      241 taacatctga gcaactgaaa ccacatcaac aaaaaccaaa ttttatcatc aaggttccaa
      301 agggcaccag ggatttaagt cctcaacaga tggttgtgag ggagaaaatt cttgataaga
      361 ttattagctg ttttaaacgt catggggcaa aggggttgga cacgccagca tttgagctaa
      421 aagaaatgct caccgagaag tatgaagaca acttcggcct catgtatgat ttggaggatc
      481 aaggtgggga gttgctgtct ctgcgctatg accttactgt cccttttgct cgctatctgg
      541 ccatgaataa attgaagagg atgaaacggt atcaagtggg aaaggtgtgg aggcgagaga
      601 gtccagctat agtccagggc cgctaccggg agttctgcca gtgtgatttt gacattgctg
      661 gtgagtttga cccaatgatc cctgatgcag agtgtttgag gattatgtgt gaaatcctaa
      721 gtggattgca gctgggggac tttctcatta aggtaaatga ccgtcgggtt gtagacggga
      781 tgtttgctgt ctgtggtgtt cctgagagca agttccgtac catctgctcc tcaatggaca
      841 aactagacaa gatgtcttgg gaagatgtga gacttgagat ggtggcaggg aaaggcctag
      901 ctcctgaggt ggctgatcga attggggact acgtccaatg ccatgggggg atatccctgg
      961 tagaggagct gttcaaggat cccagactgt cccaaagcca gctggccttg cagggcctag
     1021 gggacctgaa gctgctcttt gaatacctga ggttatttgg aattgccgat aagatctccc
     1081 ttgatctgag tctggcccgg ggcctggact attatacagg agtcatttat gaagcagtac
     1141 tgctagagtc tccagctcag gccggaaagg agactctgaa tgtgggtagt gtggctgctg
     1201 gtgggcgcta tgacaggctg gtagcccagt ttgatcccaa gggacatagc gtgccttgtg
     1261 tggggttgag cattggagta gagagaatct tctatcttgt ggagcagaag atgaagattt
     1321 cttgtgagaa ggttcgaacc acagagaccc aagtgtttgt ggccacgccc cagaagaact
     1381 tcctccaaga gcgtttgaag ataattgcac agctttggga tgcagggatc aaggcagaga
     1441 tgctttataa aaacaaccct aaactgttaa cccagctgca ctattgtgag aaagaagaca
     1501 ttcctctaat ggtcattatt ggtgagcaag aacagaatga aggtgtcgtc aagctccgtt
     1561 cagtggccag cagagaagaa gtgaccgtta atcgagaaag tcttgtggct gaaattcaga
     1621 agcgactgtc tgagtcttga ttagtgcctg atttccatct gctgctctta gaagaaaagt
     1681 tttcagctag aaccaagttc tgacagactt tacccaagtt ggccagcatg gattttgtac
     1741 aagtgccttc tgcttctatt ctctcctgga tacagagcta cagaaggacc tgaggactcg
     1801 tggactaaca ttagcagctg ctctgttaga atgcacatgg ctgtcatgcc aaagaggcat
     1861 cctgtagctg caaaggcaga tctaaagtgc ctctgaattc tgtcaaaggg atgttaggat
     1921 ggttgctatg agaaaagctc tggaagggtt agttgcctaa ggctaagact ctgacccatt
     1981 gaaatgagaa gctaatatgt acttcacttt tgactgtagc ttaggaacca gattctggag
     2041 aatttgtcta catcaactat gggttgtcca tggagttaca tggacagtag gaatggacag
     2101 aaaaaagaga tgctatacca agggcccagc agtgcatctg ttggttcttc cagttaacag
     2161 tagaaggttg ggctacttca taattttgaa cattgaactt aatggatgat aagttagtga
     2221 tctgctcttt tgaggttttg aacaccattc catttgttat agtaattttt ctggattatt
     2281 ccatatcctg ctctggagat tgttctagaa gtggttatat gataggttac atttcaaaaa
     2341 tgtactagtg ggctgcagag atggctcact gattaagggg gcatattgct tttccagagg
     2401 ccctgagttc agttcctagc aaccacatca gagtgattac aactgcctgt aactccagta
     2461 acagttatct gaggccctct tctggcctcc acaaacacct gcacttaatg tgcacatact
     2521 tatgcacaga cacatacata taattaagaa taaaataatt ttaaaaaata aacacacttt
     2581 gagtaatttg tcaccgaagg attaatctgt gtaggcttta ggaaatgttt gagtgtaatt
     2641 tggagaatcg tgtgagttac gagtaaccta ccctggggcc cacatggtag aagaagagac
     2701 agattttctt gtttgtcctc tggcctctac ccacttacca tgacttgtaa agcacacacg
     2761 caggcttata tacaaaataa agtataaaag acaaaacaac ccaccaaaac accaacatca
     2821 agtgtggcta cggacatggc tctcttgata gaatgtctgc ctagcacctg ggaagtgaag
     2881 caagagatca gaagttcaaa gtcagctttg gcgccaggca aggtggcaca ggactttaat
     2941 cctagtccaa gggaagtaaa gacaggccaa ttatctgtgt tgtaggttag ccatggctac
     3001 atagtgaaac cctgtataaa aattcattca gccttgggtt tacatgagat gctatgtcaa
     3061 taaaacaaaa gaaaaaccct ttgcctgaa
//
