LOCUS       NM_177982               3104 bp    mRNA    linear   ROD 31-MAR-2024
DEFINITION  Rattus norvegicus huntingtin-associated protein 1 (Hap1),
            transcript variant 2, mRNA.
ACCESSION   NM_177982
VERSION     NM_177982.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 3104)
  AUTHORS   Wen,Y., Zhang,G., Liu,L., Zhang,P., Lin,L., Mei,R., Zhang,F.,
            Chen,Y. and Li,R.
  TITLE     HAP1 interacts with 14-3-3 to regulate epileptic seizure via
            GABAAR-mediated inhibitory synaptic transmission in
            pentylenetetrazole rat model
  JOURNAL   Neurosci Res 182, 7-14 (2022)
   PUBMED   35609730
  REMARK    GeneRIF: HAP1 interacts with 14-3-3 to regulate epileptic seizure
            via GABAAR-mediated inhibitory synaptic transmission in
            pentylenetetrazole rat model.
REFERENCE   2  (bases 1 to 3104)
  AUTHORS   Yanai,A., Islam,M.N., Hayashi-Okada,M., Jahan,M.R., Tarif,A.M.M.,
            Nozaki,K., Masumoto,K.H. and Shinoda,K.
  TITLE     Immunohistochemical relationships of huntingtin-associated protein
            1 with enteroendocrine cells in the pyloric mucosa of the rat
            stomach
  JOURNAL   Acta Histochem 122 (8), 151650 (2020)
   PUBMED   33161374
  REMARK    GeneRIF: Immunohistochemical relationships of huntingtin-associated
            protein 1 with enteroendocrine cells in the pyloric mucosa of the
            rat stomach.
REFERENCE   3  (bases 1 to 3104)
  AUTHORS   Islam,M.N., Takeshita,Y., Yanai,A., Imagawa,A., Jahan,M.R.,
            Wroblewski,G., Nemoto,J., Fujinaga,R. and Shinoda,K.
  TITLE     Immunohistochemical analysis of huntingtin-associated protein 1 in
            adult rat spinal cord and its regional relationship with androgen
            receptor
  JOURNAL   Neuroscience 340, 201-217 (2017)
   PUBMED   27984179
  REMARK    GeneRIF: Study demonstrated that HAP1 is abundantly expressed in
            spinal neurons of the somatosensory, viscerosensory, and autonomic
            regions but absent in somatomotor neurons, suggesting that the
            spinal motoneurons are, due to lack of putative HAP1 protectivity,
            more vulnerable to stresses in neurodegenerative diseases than
            other HAP1-expressing neurons probably involved in spinal sensory
            and autonomic functions.
REFERENCE   4  (bases 1 to 3104)
  AUTHORS   Mele,M., Aspromonte,M.C. and Duarte,C.B.
  TITLE     Downregulation of GABAA Receptor Recycling Mediated by HAP1
            Contributes to Neuronal Death in In Vitro Brain Ischemia
  JOURNAL   Mol Neurobiol 54 (1), 45-57 (2017)
   PUBMED   26732589
  REMARK    GeneRIF: A key role for HAP1 protein was determined in the
            down-regulation Of GABA A receptor neurotransmission during
            cerebral ischemia.
REFERENCE   5  (bases 1 to 3104)
  AUTHORS   Vital,M., Bidegain,E., Raggio,V. and Esperon,P.
  TITLE     Molecular characterization of genes modifying the age at onset in
            Huntington's disease in Uruguayan patients
  JOURNAL   Int J Neurosci 126 (6), 510-513 (2016)
   PUBMED   26000918
REFERENCE   6  (bases 1 to 3104)
  AUTHORS   Li,S.H., Hosseini,S.H., Gutekunst,C.A., Hersch,S.M., Ferrante,R.J.
            and Li,X.J.
  TITLE     A human HAP1 homologue. Cloning, expression, and interaction with
            huntingtin
  JOURNAL   J Biol Chem 273 (30), 19220-19227 (1998)
   PUBMED   9668110
  REMARK    Erratum:[J Biol Chem 1999 Apr 2;274(14):9906]
REFERENCE   7  (bases 1 to 3104)
  AUTHORS   Li,S.H., Gutekunst,C.A., Hersch,S.M. and Li,X.J.
  TITLE     Interaction of huntingtin-associated protein with dynactin
            P150Glued
  JOURNAL   J Neurosci 18 (4), 1261-1269 (1998)
   PUBMED   9454836
REFERENCE   8  (bases 1 to 3104)
  AUTHORS   Engelender,S., Sharp,A.H., Colomer,V., Tokito,M.K., Lanahan,A.,
            Worley,P., Holzbaur,E.L. and Ross,C.A.
  TITLE     Huntingtin-associated protein 1 (HAP1) interacts with the p150Glued
            subunit of dynactin
  JOURNAL   Hum Mol Genet 6 (13), 2205-2212 (1997)
   PUBMED   9361024
REFERENCE   9  (bases 1 to 3104)
  AUTHORS   Colomer,V., Engelender,S., Sharp,A.H., Duan,K., Cooper,J.K.,
            Lanahan,A., Lyford,G., Worley,P. and Ross,C.A.
  TITLE     Huntingtin-associated protein 1 (HAP1) binds to a Trio-like
            polypeptide, with a rac1 guanine nucleotide exchange factor domain
  JOURNAL   Hum Mol Genet 6 (9), 1519-1525 (1997)
   PUBMED   9285789
REFERENCE   10 (bases 1 to 3104)
  AUTHORS   Li,X.J., Li,S.H., Sharp,A.H., Nucifora,F.C. Jr., Schilling,G.,
            Lanahan,A., Worley,P., Snyder,S.H. and Ross,C.A.
  TITLE     A huntingtin-associated protein enriched in brain with implications
            for pathology
  JOURNAL   Nature 378 (6555), 398-402 (1995)
   PUBMED   7477378
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from JAXUCZ010000010.1.
            
            On Nov 20, 2020 this sequence version replaced NM_177982.2.
            
            Summary: Huntington's disease (HD), a neurodegenerative disorder
            characterized by loss of striatal neurons, is caused by an
            expansion of a polyglutamine tract in the HD protein huntingtin.
            This gene encodes a protein that is homologous to the human
            huntingtin-associated protein 1. The human protein interacts with
            huntingtin, with two cytoskeletal proteins (dynactin and
            pericentriolar autoantigen protein 1), and with a hepatocyte growth
            factor-regulated tyrosine kinase substrate. The interactions with
            cytoskeletal proteins and a kinase substrate suggest a role for
            this protein in vesicular trafficking or organelle transport. Two
            transcripts encoding different isoforms have been identified.
            [provided by RefSeq, Jul 2008].
            
            Transcript Variant: This variant (2) lacks a segment in the 3'
            coding region that causes a frameshift, and lacks a segment in the
            3' UTR, compared to variant 1. The encoded protein (isoform B) is
            longer and has a distinct C-terminus, compared to isoform A.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR26643295.40746.1,
                                           SRR22399481.667270.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA5760389,
                                           SAMEA5760393 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            NMD candidate          :: PMID: 7477378
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-413               JAXUCZ010000010.1  85786091-85786503   c
            414-490             JAXUCZ010000010.1  85785075-85785151   c
            491-607             JAXUCZ010000010.1  85784632-85784748   c
            608-786             JAXUCZ010000010.1  85784341-85784519   c
            787-886             JAXUCZ010000010.1  85784096-85784195   c
            887-953             JAXUCZ010000010.1  85783924-85783990   c
            954-1084            JAXUCZ010000010.1  85782557-85782687   c
            1085-1147           JAXUCZ010000010.1  85782208-85782270   c
            1148-1282           JAXUCZ010000010.1  85781792-85781926   c
            1283-1374           JAXUCZ010000010.1  85781608-85781699   c
            1375-1757           JAXUCZ010000010.1  85780121-85780503   c
            1758-1965           JAXUCZ010000010.1  85779536-85779743   c
            1966-3104           JAXUCZ010000010.1  85778267-85779405   c
FEATURES             Location/Qualifiers
     source          1..3104
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="10"
                     /map="10q31"
     gene            1..3104
                     /gene="Hap1"
                     /gene_synonym="HAP1-A; HAP1-B"
                     /note="huntingtin-associated protein 1"
                     /db_xref="GeneID:29430"
                     /db_xref="RGD:68327"
     exon            1..413
                     /gene="Hap1"
                     /gene_synonym="HAP1-A; HAP1-B"
                     /inference="alignment:Splign:2.1.0"
     CDS             23..1912
                     /gene="Hap1"
                     /gene_synonym="HAP1-A; HAP1-B"
                     /note="isoform B is encoded by transcript variant 2;
                     HAP-1"
                     /codon_start=1
                     /product="huntingtin-associated protein 1 isoform B"
                     /protein_id="NP_817091.2"
                     /db_xref="GeneID:29430"
                     /db_xref="RGD:68327"
                     /translation="MRPKEQVQSSAGDGTGSGDPATGTPTTQPAADPAPEPSAEPKPA
                     PAQGTGSGQKSGSRTKTGGSFCRSRIRGDSDAPWTRYIFQGPYGPRATGLGTGRAEGI
                     WKTPAAYIGRRPGVSGPERAAFIRELQEALCPNPLPRKKITEDDIKVMLYLLEEKERD
                     LNTAARIGQSLVKQNSVLMEENNKLETMLGSAREEILHLRKQVNLRDDLLQLYSDSDD
                     DEEDEEDEEEEEGEEEEREGQRDQDQQHDHPYGAPKPPPKAETLHHCPQLEALKQKLK
                     LLEEENDHLREEASHLDNLEDKEQMLILECVEQFSEASQQMAELSEVLVLRLEGYERQ
                     QKEITQLQAEITKLQQRCQSYGAQTEKLQQQLASEKGVHPESLRAGSHMQDYGSRPRE
                     RQEDGKSHRQRSSMPAGSVTHYGYSVPLDALPSFPETLAEELRTSLRKFITDPAYFME
                     RCDTRCREERKKEQGTMPPPPVQDLKPPEDFEAPEELVPEEELGAIEEVGTAEDGPAE
                     ETEQASEETEAWEEVEPEVDEATRMNVVVSALEASGLGPSHLDMKYVLQQLSNWQDAH
                     SKRQQKQKVVPKDSPAPQQQTNMGGGIVEQQPIVPTQDSQRLEEDRATHSPSAREEEG
                     PSGAT"
     exon            414..490
                     /gene="Hap1"
                     /gene_synonym="HAP1-A; HAP1-B"
                     /inference="alignment:Splign:2.1.0"
     exon            491..607
                     /gene="Hap1"
                     /gene_synonym="HAP1-A; HAP1-B"
                     /inference="alignment:Splign:2.1.0"
     exon            608..786
                     /gene="Hap1"
                     /gene_synonym="HAP1-A; HAP1-B"
                     /inference="alignment:Splign:2.1.0"
     exon            787..886
                     /gene="Hap1"
                     /gene_synonym="HAP1-A; HAP1-B"
                     /inference="alignment:Splign:2.1.0"
     exon            887..953
                     /gene="Hap1"
                     /gene_synonym="HAP1-A; HAP1-B"
                     /inference="alignment:Splign:2.1.0"
     exon            954..1084
                     /gene="Hap1"
                     /gene_synonym="HAP1-A; HAP1-B"
                     /inference="alignment:Splign:2.1.0"
     exon            1085..1147
                     /gene="Hap1"
                     /gene_synonym="HAP1-A; HAP1-B"
                     /inference="alignment:Splign:2.1.0"
     exon            1148..1282
                     /gene="Hap1"
                     /gene_synonym="HAP1-A; HAP1-B"
                     /inference="alignment:Splign:2.1.0"
     exon            1283..1374
                     /gene="Hap1"
                     /gene_synonym="HAP1-A; HAP1-B"
                     /inference="alignment:Splign:2.1.0"
     exon            1375..1757
                     /gene="Hap1"
                     /gene_synonym="HAP1-A; HAP1-B"
                     /inference="alignment:Splign:2.1.0"
     exon            1758..1965
                     /gene="Hap1"
                     /gene_synonym="HAP1-A; HAP1-B"
                     /inference="alignment:Splign:2.1.0"
     exon            1966..3104
                     /gene="Hap1"
                     /gene_synonym="HAP1-A; HAP1-B"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gacagcggac tgcagagtca agatgcgccc gaaggagcag gtgcagagca gtgccgggga
       61 cgggacgggg tcgggggacc cagcaacagg cacccccacg acccagcctg cagcggatcc
      121 cgctccggag ccctcggcag agcccaaacc tgctccggcg cagggaaccg ggtccggaca
      181 gaaatcagga tcccgaacca agacaggagg aagcttttgt aggtccagga tccgtggcga
      241 ctcggacgca ccatggaccc gctacatatt ccaggggcct tacggtcccc gggctactgg
      301 cctgggcact ggaagggctg agggaatctg gaagacgcca gccgcgtaca tcggccgaag
      361 gcccggcgtg tccggccctg agcgcgcggc gtttattcga gagctgcagg aagcgctgtg
      421 tcctaatcca ctgcccagga agaagatcac cgaagatgat atcaaagtga tgttgtattt
      481 gctggaagag aaagaacggg acctgaacac agccgctcgc atcggccagt ccctggtgaa
      541 acagaatagt gttctgatgg aggagaataa caagctggaa accatgctgg gctcagccag
      601 agaggagatt ttacatctcc ggaagcaggt gaacctgcga gatgatctcc ttcagctcta
      661 ctcggactcc gatgacgatg aggaggatga agaggatgag gaagaggaag agggagaaga
      721 ggaggaacga gaaggacaga gggaccaaga tcagcagcac gaccatccct atggtgcccc
      781 caagccgccc cctaaggctg agacgctgca ccactgccca cagctggaag ccctgaagca
      841 gaagctgaaa ctgctggaag aagagaacga ccatcttcga gaggaggcct cccaccttga
      901 caacctggaa gacaaagaac agatgctcat tctggagtgt gtggaacagt tttctgaagc
      961 cagccagcag atggcagagc tatccgaggt gttggtgctg aggctggaag gctatgagag
     1021 gcagcagaag gagatcactc agctgcaggc cgagatcacc aagctacaac agcgttgtca
     1081 gtcttatggg gcccagacgg agaaactgca gcagcagctg gcctcagaga agggagtcca
     1141 cccagagagc ctgcgagctg gctcccacat gcaggattat ggaagcaggc ctcgtgaacg
     1201 ccaggaggat gggaagagcc atcgtcagcg ttcctcaatg cctgcaggtt ctgtcaccca
     1261 ctatggatac agtgtgcctc tggatgcact tccaagtttc ccagagacac tggcggagga
     1321 gctccggaca tccctgagga agttcatcac tgaccctgcg tatttcatgg agagatgtga
     1381 cactcgctgc agagaggaac gaaagaagga gcaggggaca atgccacccc caccggtgca
     1441 agatctcaag ccgcctgaag atttcgaggc tccagaggag ctggttcctg aggaggagct
     1501 gggggccata gaagaggtgg ggacagctga ggatgggccg gcagaagaga cagagcaggc
     1561 atctgaggag accgaggcct gggaggaggt ggaaccggag gtggacgagg ccacaaggat
     1621 gaatgtggtg gtctctgccc tggaggccag cggcctgggc ccttcacacc tggacatgaa
     1681 gtatgtcctc cagcaactgt ccaactggca ggacgcccat tctaagcggc agcagaagca
     1741 gaaggtggtc ccgaaagact ccccagcccc gcagcagcaa acaaacatgg ggggcgggat
     1801 cgtggagcag cagcccatag tgccgaccca ggactctcag aggctggagg aggacagggc
     1861 cactcactct cccagtgcca gggaggaaga ggggccttct ggggccacct aggcctggaa
     1921 cgaaggcctc tgccagcaaa ggccacagtt ggaccaatcc cccggctcct ttcgtcttct
     1981 ggccctgtgg gtgggtggat gtgattgtgc accatttatt agaagaggaa tctgaagttc
     2041 agagatctga gatgacttgg ccaaggtcat acagctagga gcagatctga gccggtgtct
     2101 cttgaggact gaaaccctgg cctggtctca ctgccgtcca cacctcctcc tgctcccgtc
     2161 cttgagctcc acctgaagct ttggcagcct ccctcatgtc ggtctgttgc catttttggg
     2221 gggcaccagt gagtggaaca tcactggctg cagcacagtc agatcatgca ggttgtaagc
     2281 tgagtcagca cacccgtggc tgaccacggg agaccgggag acctgagtaa cggctagcgg
     2341 tggctttctg ttgtctcctg acccggctga ctgggtttga gggtgatctg tctcccggtt
     2401 cacccccttc tcttcctggc tttgctgttt ctcagcagcc tgaagaagac cctcagacag
     2461 ctcgagaccc ctccttccac tgcctggcca agtccgaccc ttccttgctt tcctctgaga
     2521 caggaccccc acccccatgt ctgtcagcag ttctccctct gtcttgatag atctttcccc
     2581 tcttgtagga caggctgaaa gaaccccagt ccctcattct gaaactgggg ccaaagctgt
     2641 gcctgctgga gccccagggg gtggaagcaa cctgtgggta gattggttgg ttaccagacc
     2701 cagctctgag atggtgtggg tgcgcacatc cctagggtag gcagttattg gggggaccct
     2761 ttctatccct tgaacctctc acgtaaaggg acttccccag tcctggttgg cttttggaac
     2821 ctggtccttc ttgctgtttt ttacccttcc ccgtttctat tactgcgtgt aacgtaaagt
     2881 gtatctgagt gagggtggtg ggaaccctct gtccagtgct gtctctgtct cctatggcct
     2941 gtgagtttcc ttctagagtt ctactcttct ccacctcttt gctcatacag agctgtggcc
     3001 ttggcgcctg ccctgcttct gcagtgcttc attgtctcgt agcttgtcag cttttgtatt
     3061 gatgtatgtg ccaatgtggg aaataaagac cttgtgagat acct
//
