LOCUS       NM_032082               1647 bp    mRNA    linear   ROD 03-APR-2024
DEFINITION  Rattus norvegicus hydroxyacid oxidase 2 (Hao2), mRNA.
ACCESSION   NM_032082
VERSION     NM_032082.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 1647)
  AUTHORS   Mattu,S., Fornari,F., Quagliata,L., Perra,A., Angioni,M.M.,
            Petrelli,A., Menegon,S., Morandi,A., Chiarugi,P.,
            Ledda-Columbano,G.M., Gramantieri,L., Terracciano,L., Giordano,S.
            and Columbano,A.
  TITLE     The metabolic gene HAO2 is downregulated in hepatocellular
            carcinoma and predicts metastasis and poor survival
  JOURNAL   J Hepatol 64 (4), 891-898 (2016)
   PUBMED   26658681
  REMARK    GeneRIF: Dysregulation of HAO2 is a very early event in the
            development of hepatocellular carcinoma (HCC).
REFERENCE   2  (bases 1 to 1647)
  AUTHORS   Ghosh,D. and Berg,J.M.
  TITLE     A proteome-wide perspective on peroxisome targeting signal
            1(PTS1)-Pex5p affinities
  JOURNAL   J Am Chem Soc 132 (11), 3973-3979 (2010)
   PUBMED   20178365
REFERENCE   3  (bases 1 to 1647)
  AUTHORS   Cunane,L.M., Barton,J.D., Chen,Z.W., Le,K.H., Amar,D., Lederer,F.
            and Mathews,F.S.
  TITLE     Crystal structure analysis of recombinant rat kidney long chain
            hydroxy acid oxidase
  JOURNAL   Biochemistry 44 (5), 1521-1531 (2005)
   PUBMED   15683236
REFERENCE   4  (bases 1 to 1647)
  AUTHORS   Kikuchi,M., Hatano,N., Yokota,S., Shimozawa,N., Imanaka,T. and
            Taniguchi,H.
  TITLE     Proteomic analysis of rat liver peroxisome: presence of
            peroxisome-specific isozyme of Lon protease
  JOURNAL   J Biol Chem 279 (1), 421-428 (2004)
   PUBMED   14561759
REFERENCE   5  (bases 1 to 1647)
  AUTHORS   Lee,S.J., Liu,J., Qi,N., Guarnera,R.A., Lee,S.Y. and Cicila,G.T.
  TITLE     Use of a panel of congenic strains to evaluate differentially
            expressed genes as candidate genes for blood pressure quantitative
            trait loci
  JOURNAL   Hypertens Res 26 (1), 75-87 (2003)
   PUBMED   12661916
REFERENCE   6  (bases 1 to 1647)
  AUTHORS   Jones,J.M., Morrell,J.C. and Gould,S.J.
  TITLE     Identification and characterization of HAOX1, HAOX2, and HAOX3,
            three human peroxisomal 2-hydroxy acid oxidases
  JOURNAL   J Biol Chem 275 (17), 12590-12597 (2000)
   PUBMED   10777549
REFERENCE   7  (bases 1 to 1647)
  AUTHORS   Belmouden,A., Le,K.H., Lederer,F. and Garchon,H.J.
  TITLE     Molecular cloning and nucleotide sequence of cDNA encoding rat
            kidney long-chain L-2-hydroxy acid oxidase. Expression of the
            catalytically active recombinant protein as a chimaera
  JOURNAL   Eur J Biochem 214 (1), 17-25 (1993)
   PUBMED   8508789
REFERENCE   8  (bases 1 to 1647)
  AUTHORS   Diep Le,K.H. and Lederer,F.
  TITLE     Amino acid sequence of long chain alpha-hydroxy acid oxidase from
            rat kidney, a member of the family of FMN-dependent alpha-hydroxy
            acid-oxidizing enzymes
  JOURNAL   J Biol Chem 266 (31), 20877-20881 (1991)
   PUBMED   1939137
REFERENCE   9  (bases 1 to 1647)
  AUTHORS   Urban,P., Chirat,I. and Lederer,F.
  TITLE     Rat kidney L-2-hydroxyacid oxidase. Structural and mechanistic
            comparison with flavocytochrome b2 from baker's yeast
  JOURNAL   Biochemistry 27 (19), 7365-7371 (1988)
   PUBMED   3061453
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from X67156.1.
            
            On Aug 30, 2012 this sequence version replaced NM_032082.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: X67156.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5756307, SAMEA5760400
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1647              X67156.1           2-1648
FEATURES             Location/Qualifiers
     source          1..1647
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="W"
                     /db_xref="taxon:10116"
                     /chromosome="2"
                     /map="2q34"
     gene            1..1647
                     /gene="Hao2"
                     /gene_synonym="Hao3; LCHAO"
                     /note="hydroxyacid oxidase 2"
                     /db_xref="GeneID:84029"
                     /db_xref="RGD:70972"
     exon            1..88
                     /gene="Hao2"
                     /gene_synonym="Hao3; LCHAO"
                     /inference="alignment:Splign:2.1.0"
     exon            89..193
                     /gene="Hao2"
                     /gene_synonym="Hao3; LCHAO"
                     /inference="alignment:Splign:2.1.0"
     exon            194..332
                     /gene="Hao2"
                     /gene_synonym="Hao3; LCHAO"
                     /inference="alignment:Splign:2.1.0"
     CDS             202..1263
                     /gene="Hao2"
                     /gene_synonym="Hao3; LCHAO"
                     /EC_number="1.1.3.15"
                     /note="HAOX2; long-chain L-2-hydroxy acid oxidase; long
                     chain alpha-hydroxy acid oxidase; (S)-2-hydroxy-acid
                     oxidase, peroxisomal; hydroxyacid oxidase 3
                     (medium-chain); hydroxyacid oxidase 2 (long chain)"
                     /codon_start=1
                     /product="2-Hydroxyacid oxidase 2"
                     /protein_id="NP_114471.1"
                     /db_xref="GeneID:84029"
                     /db_xref="RGD:70972"
                     /translation="MPLVCLADFKAHAQKQLSKTSWDFIEGEADDGITYSENIAAFKR
                     IRLRPRYLRDMSKVDTRTTIQGQEISAPICISPTAFHSIAWPDGEKSTARAAQEANIC
                     YVISSYASYSLEDIVAAAPEGFRWFQLYMKSDWDFNKQMVQRAEALGFKALVITIDTP
                     VLGNRRRDKRNQLNLEANILLKDLRALKEEKPTQSVPVSFPKASFCWNDLSLLQSITR
                     LPIILKGILTKEDAELAMKHNVQGIVVSNHGGRQLDEVSASIDALREVVAAVKGKIEV
                     YMDGGVRTGTDVLKALALGARCIFLGRPILWGLACKGEDGVKEVLDILTAELHRCMTL
                     SGCQSVAEISPDLIQFSRL"
     exon            333..484
                     /gene="Hao2"
                     /gene_synonym="Hao3; LCHAO"
                     /inference="alignment:Splign:2.1.0"
     exon            485..768
                     /gene="Hao2"
                     /gene_synonym="Hao3; LCHAO"
                     /inference="alignment:Splign:2.1.0"
     exon            769..978
                     /gene="Hao2"
                     /gene_synonym="Hao3; LCHAO"
                     /inference="alignment:Splign:2.1.0"
     exon            979..1137
                     /gene="Hao2"
                     /gene_synonym="Hao3; LCHAO"
                     /inference="alignment:Splign:2.1.0"
     exon            1138..1207
                     /gene="Hao2"
                     /gene_synonym="Hao3; LCHAO"
                     /inference="alignment:Splign:2.1.0"
     exon            1208..1647
                     /gene="Hao2"
                     /gene_synonym="Hao3; LCHAO"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 agagctgggc aatgaaccac tgtagcgggc tttgcattgc tgcctctacc tacagctatt
       61 actggtcatt ggaagacctt agagtcagaa tcttcttgtg taagagccct gaatgctgtg
      121 accaacccca gtgtctacag catctttgca gctgttaatc tcactgttct cgttcctatt
      181 gaagaaatta ctggcccaga aatgcctttg gtgtgtttgg cagactttaa ggcacatgcg
      241 caaaagcagc tgtctaagac ctcctgggac tttattgaag gagaagctga cgacggcatc
      301 acctacagtg agaacatagc agcatttaaa agaatccgcc tccgcccccg atacctgaga
      361 gatatgtcaa aggtggacac caggaccaca atccaagggc aggagatcag tgctcccatc
      421 tgcatctcac ccacagcctt tcactccatt gcctggccgg atggagaaaa gagcacagct
      481 agagctgctc aggaggccaa catctgctat gtcatcagca gttatgccag ctattccctg
      541 gaagatattg ttgctgctgc ccccgaaggc tttcgttggt tccaactcta catgaagtca
      601 gactgggact tcaacaagca gatggttcag agggcagaag ccttgggttt caaagctttg
      661 gtgatcacta tagatacgcc tgtacttggc aataggcgac gggacaagag aaaccagctg
      721 aatttggagg caaacatatt gttgaaggat ctccgagccc tcaaagagga aaagcccaca
      781 cagtctgtgc ccgtgtcttt tccgaaagca tctttctgct ggaatgatct ttccttgctt
      841 cagagtataa ctcggttgcc cattatcctc aaagggattt tgacgaaaga ggatgcagag
      901 ttagcaatga agcacaacgt ccaaggcatc gttgtttcca accatggtgg gaggcagctt
      961 gatgaggttt ctgcttcaat tgatgctctg agagaagtgg tggctgctgt caaagggaaa
     1021 attgaagtgt acatggatgg tggggttcga actggcactg atgtgttgaa ggcactggcc
     1081 cttggagcta ggtgcatttt tcttgggaga ccaatccttt ggggccttgc ctgcaagggt
     1141 gaagatggtg ttaaggaagt tttagatatt ctaacagcag aactccatag atgtatgacc
     1201 ctttcaggct gccagtcagt tgctgagatt agtccagacc tgattcagtt ctccagatta
     1261 taaggaccta ctgagatccc tacaagagga agacaagact tcaacatagt gtgtgagcct
     1321 attcttcttc ggtccgatca tacctagtag tttgagccct ctaccttgag aaatccagat
     1381 cgatgaagaa aagatagcta acagctacca gaggggtgca tttggatgaa ggaataacat
     1441 ctaatgttct acaggataac tataactgac aattaattga ctatgtccaa tagccatgtt
     1501 ccagaaagaa aggaaatagt tacatctggt gtgacatgcc aattactttc atttgattat
     1561 tatgtacact atacattatt aaaacgtcat actgtgccat aattatgtgc aattactatg
     1621 tatcaattaa aacaacaata tatattt
//
