LOCUS       NM_001270711            2452 bp    mRNA    linear   ROD 03-APR-2024
DEFINITION  Rattus norvegicus guanylate cyclase 1 soluble subunit beta 2
            (Gucy1b2), transcript variant 1, mRNA.
ACCESSION   NM_001270711
VERSION     NM_001270711.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 2452)
  AUTHORS   Winger,J.A., Derbyshire,E.R. and Marletta,M.A.
  TITLE     Dissociation of nitric oxide from soluble guanylate cyclase and
            heme-nitric oxide/oxygen binding domain constructs
  JOURNAL   J Biol Chem 282 (2), 897-907 (2007)
   PUBMED   17098738
  REMARK    GeneRIF: for the function of sGC in vivo, where there is evidence
            for two nitric oxide-regulated activity states
REFERENCE   2  (bases 1 to 2452)
  AUTHORS   Karow,D.S., Pan,D., Davis,J.H., Behrends,S., Mathies,R.A. and
            Marletta,M.A.
  TITLE     Characterization of functional heme domains from soluble guanylate
            cyclase
  JOURNAL   Biochemistry 44 (49), 16266-16274 (2005)
   PUBMED   16331987
  REMARK    GeneRIF: Results identify and characterize the minimum functional
            ligand-binding heme domain derived from soluble guanylate cyclase.
REFERENCE   3  (bases 1 to 2452)
  AUTHORS   Lin,L.H. and Talman,W.T.
  TITLE     Soluble guanylate cyclase and neuronal nitric oxide synthase
            colocalize in rat nucleus tractus solitarii
  JOURNAL   J Chem Neuroanat 29 (2), 127-136 (2005)
   PUBMED   15652699
  REMARK    GeneRIF: These data support our hypothesis and provide anatomical
            support for the suggestion that nitroxidergic activation of the NTS
            involves sGC.
REFERENCE   4  (bases 1 to 2452)
  AUTHORS   Okamoto,H.
  TITLE     Molecular cloning of a novel variant of the rat soluble guanylate
            cyclase beta2 subunit
  JOURNAL   Int J Biochem Cell Biol 36 (3), 472-480 (2004)
   PUBMED   14687925
REFERENCE   5  (bases 1 to 2452)
  AUTHORS   Behrends,S., Budaeus,L., Kempfert,J., Scholz,H., Starbatty,J. and
            Vehse,K.
  TITLE     The beta 2 subunit of nitric oxide-sensitive guanylyl cyclase is
            developmentally regulated in rat kidney
  JOURNAL   Naunyn Schmiedebergs Arch Pharmacol 364 (6), 573-576 (2001)
   PUBMED   11770014
  REMARK    GeneRIF: Expression of the beta 2 subunit of soluble guanylyl
            cyclase is switched on at a time when the urinary concentrating
            capacity develops, between postnatal days 16 to 22, increasing
            strongly in intensity in adult animals.
REFERENCE   6  (bases 1 to 2452)
  AUTHORS   Koglin,M., Vehse,K., Budaeus,L., Scholz,H. and Behrends,S.
  TITLE     Nitric oxide activates the beta 2 subunit of soluble guanylyl
            cyclase in the absence of a second subunit
  JOURNAL   J Biol Chem 276 (33), 30737-30743 (2001)
   PUBMED   11406623
REFERENCE   7  (bases 1 to 2452)
  AUTHORS   Azam,M., Gupta,G., Chen,W., Wellington,S., Warburton,D. and
            Danziger,R.S.
  TITLE     Genetic mapping of soluble guanylyl cyclase genes: implications for
            linkage to blood pressure in the Dahl rat
  JOURNAL   Hypertension 32 (1), 149-154 (1998)
   PUBMED   9674652
REFERENCE   8  (bases 1 to 2452)
  AUTHORS   Sangermani,R., Gibelli,M., Vaglia,P. and Vaccari,R.
  TITLE     [Changes in the consciousness state as a symptom of intestinal
            invagination onset]
  JOURNAL   Pediatr Med Chir 14 (6), 623-625 (1992)
   PUBMED   1298938
REFERENCE   9  (bases 1 to 2452)
  AUTHORS   Yuen,P.S., Potter,L.R. and Garbers,D.L.
  TITLE     A new form of guanylyl cyclase is preferentially expressed in rat
            kidney
  JOURNAL   Biochemistry 29 (49), 10872-10878 (1990)
   PUBMED   1980215
REFERENCE   10 (bases 1 to 2452)
  AUTHORS   Arnold,W.P., Mittal,C.K., Katsuki,S. and Murad,F.
  TITLE     Nitric oxide activates guanylate cyclase and increases guanosine
            3':5'-cyclic monophosphate levels in various tissue preparations
  JOURNAL   Proc Natl Acad Sci U S A 74 (8), 3203-3207 (1977)
   PUBMED   20623
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AY004153.2, M57507.1 and AB109451.1.
            
            Transcript Variant: This variant (1) encodes the longer isoform
            (1).
            
            ##Evidence-Data-START##
            Transcript exon combination :: AY004153.2 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA5756307,
                                           SAMEA5760393 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-164               AY004153.2         1-164
            165-1201            M57507.1           48-1084
            1202-2312           AB109451.1         939-2049
            2313-2452           AY004153.2         2313-2452
FEATURES             Location/Qualifiers
     source          1..2452
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="Sprague-Dawley"
                     /db_xref="taxon:10116"
                     /chromosome="15"
                     /map="15p12"
     gene            1..2452
                     /gene="Gucy1b2"
                     /gene_synonym="Gucy1b2a; Gucy1b2b; SGC"
                     /note="guanylate cyclase 1 soluble subunit beta 2"
                     /db_xref="GeneID:25206"
                     /db_xref="RGD:2770"
     exon            1..86
                     /gene="Gucy1b2"
                     /gene_synonym="Gucy1b2a; Gucy1b2b; SGC"
                     /inference="alignment:Splign:2.1.0"
     CDS             84..2312
                     /gene="Gucy1b2"
                     /gene_synonym="Gucy1b2a; Gucy1b2b; SGC"
                     /EC_number="4.6.1.2"
                     /note="isoform 1 is encoded by transcript variant 1;
                     guanylate cyclase soluble subunit beta-2; soluble
                     guanylate cyclase beta 2b; soluble guanylate cyclase beta
                     2a; GCS-beta-2; Guanylate cyclase, soluble, beta 2 (GTP
                     pyrophosphate - lyase); guanylate cyclase 1, soluble, beta
                     2"
                     /codon_start=1
                     /product="guanylate cyclase soluble subunit beta-2 isoform
                     1"
                     /protein_id="NP_001257640.1"
                     /db_xref="GeneID:25206"
                     /db_xref="RGD:2770"
                     /translation="MYGFINTCLQSLVTEKFGEETWEKLKASAEVQDVFMTYTVYDDI
                     ITIKLIQEACKVLDVSMEAILKLFGEYFFKFCKMSGYDRMLRTLGGNLTEFIENLDAL
                     HSYLALSYQEMNAPSFRVEEGADGAMLLHYYSDRHGLCHIVPGIIEAVAKDFFDTDVA
                     MSILDMNEEVERTGKKEHVVFLVVQKAHRQIRGAKASRPQGSEDSQADQEALQGTLLR
                     MKERYLNIPVCPGEKSHSTAVRASVLFGKGPLRDTFQPVYPERLWVEEEVFCDAFPFH
                     IVFDEALRVKQAGVNIQKYVPGILTQKFALDEYFSIIHPQVTFNISSICKFINSQFVL
                     KTRKEMMPKARKSQPMLKLRGQMIWMESLRCMIFMCSPKLRSLQELEESKMHLSDIAP
                     HDTTRDLILLNQQRLAEMELSCQLEKKKEELRVLSNHLAIEKKKTETLLYAMLPEHVA
                     NQLKEGRKVAAGEFETCTILFSDVVTFTNICAACEPIQIVNMLNSMYSKFDRLTSVHD
                     VYKVETIGDAYMVVGGVPVPVESHAQRVANFALGMRISAKEVMNPVTGEPIQIRVGIH
                     TGPVLAGVVGDKMPRYCLFGDTVNTASRMESHGLPSKVHLSPTAHRALKNKGFEIVRR
                     GEIEVKGKGKMTTYFLIQNLNATEDEIMGRPSAPADGKEVCTPGNQVRKSPAVPRNTD
                     HQQQVYKGDPADASNEVTLAGSPVAGRNSTDAVNNQPSPDETKTSVVASGPVLSAFCV
                     VL"
     exon            87..160
                     /gene="Gucy1b2"
                     /gene_synonym="Gucy1b2a; Gucy1b2b; SGC"
                     /inference="alignment:Splign:2.1.0"
     exon            161..255
                     /gene="Gucy1b2"
                     /gene_synonym="Gucy1b2a; Gucy1b2b; SGC"
                     /inference="alignment:Splign:2.1.0"
     exon            256..416
                     /gene="Gucy1b2"
                     /gene_synonym="Gucy1b2a; Gucy1b2b; SGC"
                     /inference="alignment:Splign:2.1.0"
     exon            417..516
                     /gene="Gucy1b2"
                     /gene_synonym="Gucy1b2a; Gucy1b2b; SGC"
                     /inference="alignment:Splign:2.1.0"
     exon            517..713
                     /gene="Gucy1b2"
                     /gene_synonym="Gucy1b2a; Gucy1b2b; SGC"
                     /inference="alignment:Splign:2.1.0"
     exon            714..825
                     /gene="Gucy1b2"
                     /gene_synonym="Gucy1b2a; Gucy1b2b; SGC"
                     /inference="alignment:Splign:2.1.0"
     exon            826..929
                     /gene="Gucy1b2"
                     /gene_synonym="Gucy1b2a; Gucy1b2b; SGC"
                     /inference="alignment:Splign:2.1.0"
     exon            930..1146
                     /gene="Gucy1b2"
                     /gene_synonym="Gucy1b2a; Gucy1b2b; SGC"
                     /inference="alignment:Splign:2.1.0"
     exon            1147..1467
                     /gene="Gucy1b2"
                     /gene_synonym="Gucy1b2a; Gucy1b2b; SGC"
                     /inference="alignment:Splign:2.1.0"
     exon            1468..1616
                     /gene="Gucy1b2"
                     /gene_synonym="Gucy1b2a; Gucy1b2b; SGC"
                     /inference="alignment:Splign:2.1.0"
     exon            1617..1763
                     /gene="Gucy1b2"
                     /gene_synonym="Gucy1b2a; Gucy1b2b; SGC"
                     /inference="alignment:Splign:2.1.0"
     exon            1764..1918
                     /gene="Gucy1b2"
                     /gene_synonym="Gucy1b2a; Gucy1b2b; SGC"
                     /inference="alignment:Splign:2.1.0"
     exon            1919..2070
                     /gene="Gucy1b2"
                     /gene_synonym="Gucy1b2a; Gucy1b2b; SGC"
                     /inference="alignment:Splign:2.1.0"
     exon            2071..2166
                     /gene="Gucy1b2"
                     /gene_synonym="Gucy1b2a; Gucy1b2b; SGC"
                     /inference="alignment:Splign:2.1.0"
     exon            2167..2452
                     /gene="Gucy1b2"
                     /gene_synonym="Gucy1b2a; Gucy1b2b; SGC"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 ctcggttgct acaagtccac atccagagac ttggcaagaa acttgctgtg cggctccagc
       61 ctggagacac atcgctggac accatgtatg gattcatcaa cacctgcctg cagtctcttg
      121 tgacagagaa atttggtgag gagacatggg agaagctgaa ggcttctgca gaagtgcaag
      181 atgtcttcat gacctacacc gtgtatgatg acatcatcac cattaagctc atccaagaag
      241 cctgcaaggt tctggatgtg tccatggaag ccattctgaa gctctttggc gaatacttct
      301 ttaagttctg taagatgtct ggctatgaca ggatgctgcg gacacttgga ggaaatctca
      361 ccgagtttat tgaaaaccta gatgcactcc acagttacct ggcactgtcc tatcaggaaa
      421 tgaacgcacc atcctttcga gtggaggaag gagctgacgg ggcgatgctt ctccactact
      481 actcagacag acatggtctg tgtcacattg taccaggtat cattgaagct gtggccaagg
      541 acttctttga cactgatgtg gccatgagta tcctggatat gaacgaagag gtggaaagga
      601 cagggaagaa agaacatgtt gtgtttctgg tcgtgcagaa ggctcacaga cagataagag
      661 gagcaaaggc aagccggcca caaggcagtg aggacagcca ggcagaccag gaggctctcc
      721 agggaacact ccttcggatg aaggagagat atttaaacat ccctgtttgc cctggggaga
      781 aatctcactc aactgctgtg agggcatcgg tcctttttgg aaaagggccc ctcagggaca
      841 ccttccagcc cgtctatcct gagagactat gggtcgaaga ggaggtgttc tgtgatgctt
      901 ttcctttcca cattgtcttt gatgaagcac taagggtcaa gcaagctgga gtgaatattc
      961 agaagtatgt ccctggaatc ttaacccaga agtttgcact agatgagtat ttttccatca
     1021 tccaccctca agttactttc aacatctcca gcatctgcaa gttcattaac agtcagtttg
     1081 tcttgaagac aagaaaagaa atgatgccca aagcaaggaa gagccagccg atgctcaaac
     1141 tccggggtca gatgatctgg atggagtctc tgaggtgcat gatcttcatg tgttccccaa
     1201 agctccgcag cctgcaagag ctggaagaga gcaagatgca tctttctgat atcgctccgc
     1261 acgacacgac cagggatctc atcctcctca accagcagag gctggcagag atggagctgt
     1321 cctgccaact ggaaaagaag aaggaggagt tgcgtgtcct ttccaatcac ctggccatcg
     1381 agaagaagaa gacagagacc ttgctgtatg ccatgctgcc tgaacatgtg gccaaccaac
     1441 tcaaggaggg cagaaaggtg gctgcaggag aatttgaaac atgtacaatc cttttcagcg
     1501 atgttgtgac atttaccaac atctgtgcag cctgtgaacc tatccaaatc gtgaacatgc
     1561 tgaattcaat gtactccaag tttgacaggt taaccagtgt ccatgatgtc tacaaagtag
     1621 aaacaatagg ggatgcttac atggtggtgg gtggagtacc agtacccgtt gaaagccatg
     1681 ctcaaagagt cgccaatttt gctctgggga tgagaatttc tgcaaaagaa gtgatgaatc
     1741 ctgtcactgg ggaacctatc cagatcagag tgggaatcca cactggacca gtcttagcag
     1801 gtgttgtggg agacaagatg cctcggtact gcttgtttgg tgacactgta aacacagcct
     1861 ctaggatgga aagtcacggg cttcccagca aagtgcatct gagccccaca gcccacagag
     1921 ccctgaaaaa caaagggttt gaaattgtca ggagaggcga gatcgaagtg aaggggaaag
     1981 gaaagatgac cacatacttt ctgatccaga acctgaatgc caccgaggat gagataatgg
     2041 ggcgaccttc agcccccgct gatgggaagg aagtatgtac tcccggaaac caagtcagga
     2101 agtcccctgc tgtcccgagg aacacagacc atcagcaaca agtctacaaa ggagacccag
     2161 cagacgcttc taatgaagtc acacttgctg ggagcccagt ggcagggcga aactccacag
     2221 atgcagtcaa taaccagcca tcaccagatg agaccaagac aagtgtcgtt gctagtggcc
     2281 ctgtgctgtc tgctttctgt gttgtgctgt gatcacgaga aaaagtgatc ctatgggatc
     2341 catttcctgt attccatggc agcaaaggga attaatttat aaaaatgctt aagttcaaaa
     2401 tgtttttgtt tccatatctc ccttggggcc cctttgagaa taaaaaaatt ag
//
