LOCUS       NM_023956               2657 bp    mRNA    linear   ROD 03-FEB-2024
DEFINITION  Rattus norvegicus guanylate cyclase 1 soluble subunit alpha 2
            (Gucy1a2), mRNA.
ACCESSION   NM_023956 XM_346814
VERSION     NM_023956.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 2657)
  AUTHORS   Gabach,L.A., Carlini,V.P., Monti,M.C., Maglio,L.E., De
            Barioglio,S.R. and Perez,M.F.
  TITLE     Involvement of nNOS/NO/sGC/cGMP signaling pathway in cocaine
            sensitization and in the associated hippocampal alterations: does
            phosphodiesterase 5 inhibition help to drug vulnerability?
  JOURNAL   Psychopharmacology (Berl) 229 (1), 41-50 (2013)
   PUBMED   23579428
  REMARK    GeneRIF: Soluble guanylyl cyclase signal pathway is involved in the
            development of cocaine sensitization.
REFERENCE   2  (bases 1 to 2657)
  AUTHORS   Velazquez,E., Blazquez,E. and Ruiz-Albusac,J.M.
  TITLE     Glucagon-like peptide-2 (GLP-2) modulates the cGMP signalling
            pathway by regulating the expression of the soluble guanylyl
            cyclase receptor subunits in cultured rat astrocytes
  JOURNAL   Mol Neurobiol 46 (2), 242-250 (2012)
   PUBMED   22806360
  REMARK    GeneRIF: The effect of glucagon-like peptide-2 on the expression of
            the alpha1, alpha2, and beta1 subunits of soluble guanylyl cyclase,
            was studied.
REFERENCE   3  (bases 1 to 2657)
  AUTHORS   Wang-Rosenke,Y., Mika,A., Khadzhynov,D., Loof,T., Neumayer,H.H. and
            Peters,H.
  TITLE     Impact of biological gender and soluble guanylate cyclase
            stimulation on renal recovery after relief of unilateral ureteral
            obstruction
  JOURNAL   J Urol 188 (1), 316-323 (2012)
   PUBMED   22608751
  REMARK    GeneRIF: In each gender group alpha1 and beta1-soluble guanylate
            cyclase was comparably and significantly increased but female rats
            produced significantly more cyclic guanosine monophosphate after
            treatment with the soluble guanylate cyclase stimulator.
REFERENCE   4  (bases 1 to 2657)
  AUTHORS   Cabilla,J.P., Ronchetti,S.A., Nudler,S.I., Miler,E.A.,
            Quinteros,F.A. and Duvilanski,B.H.
  TITLE     Nitric oxide sensitive-guanylyl cyclase subunit expression changes
            during estrous cycle in anterior pituitary glands
  JOURNAL   Am J Physiol Endocrinol Metab 296 (4), E731-E737 (2009)
   PUBMED   19141686
  REMARK    GeneRIF: Estradiol physiologically regulates sGC expression and
            activity in rat anterior pituitary gland.
REFERENCE   5  (bases 1 to 2657)
  AUTHORS   Dhaese,I., Vanneste,G., Sips,P., Buys,E., Brouckaert,P. and
            Lefebvre,R.A.
  TITLE     Involvement of soluble guanylate cyclase alpha(1) and alpha(2), and
            SK(Ca) channels in NANC relaxation of mouse distal colon
  JOURNAL   Eur J Pharmacol 589 (1-3), 251-259 (2008)
   PUBMED   18572161
  REMARK    GeneRIF: Examine the involvement of soluble guanylate cyclase
            alpha(1) and alpha(2), and SK(Ca) channels in NANC relaxation of
            mouse distal colon.
REFERENCE   6  (bases 1 to 2657)
  AUTHORS   Russwurm,M., Wittau,N. and Koesling,D.
  TITLE     Guanylyl cyclase/PSD-95 interaction: targeting of the nitric
            oxide-sensitive alpha2beta1 guanylyl cyclase to synaptic membranes
  JOURNAL   J Biol Chem 276 (48), 44647-44652 (2001)
   PUBMED   11572861
REFERENCE   7  (bases 1 to 2657)
  AUTHORS   Koglin,M. and Behrends,S.
  TITLE     Cloning and functional expression of the rat alpha(2) subunit of
            soluble guanylyl cyclase
  JOURNAL   Biochim Biophys Acta 1494 (3), 286-289 (2000)
   PUBMED   11121588
REFERENCE   8  (bases 1 to 2657)
  AUTHORS   Seebacher,T., Beitz,E., Kumagami,H., Wild,K., Ruppersberg,J.P. and
            Schultz,J.E.
  TITLE     Expression of membrane-bound and cytosolic guanylyl cyclases in the
            rat inner ear
  JOURNAL   Hear Res 127 (1-2), 95-102 (1999)
   PUBMED   9925020
REFERENCE   9  (bases 1 to 2657)
  AUTHORS   Gupta,G., Azam,M., Yang,L. and Danziger,R.S.
  TITLE     The beta2 subunit inhibits stimulation of the alpha1/beta1 form of
            soluble guanylyl cyclase by nitric oxide. Potential relevance to
            regulation of blood pressure
  JOURNAL   J Clin Invest 100 (6), 1488-1492 (1997)
   PUBMED   9294115
REFERENCE   10 (bases 1 to 2657)
  AUTHORS   Arnold,W.P., Mittal,C.K., Katsuki,S. and Murad,F.
  TITLE     Nitric oxide activates guanylate cyclase and increases guanosine
            3':5'-cyclic monophosphate levels in various tissue preparations
  JOURNAL   Proc Natl Acad Sci U S A 74 (8), 3203-3207 (1977)
   PUBMED   20623
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from AF109963.2.
            
            [WARNING] On Apr 12, 2024 this sequence was replaced by
            NM_023956.2.
            
            On Dec 17, 2003 this sequence version replaced XM_346814.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF109963.2, AB097860.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760384, SAMEA5760393
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..2657
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="WKY"
                     /db_xref="taxon:10116"
                     /chromosome="8"
                     /map="8q11"
     gene            1..2657
                     /gene="Gucy1a2"
                     /note="guanylate cyclase 1 soluble subunit alpha 2"
                     /db_xref="GeneID:66012"
                     /db_xref="RGD:621655"
     exon            1..717
                     /gene="Gucy1a2"
                     /inference="alignment:Splign:2.1.0"
     CDS             421..2613
                     /gene="Gucy1a2"
                     /EC_number="4.6.1.2"
                     /function="produces cGMP"
                     /note="nitric oxide-sensitive; GCS-alpha-2; soluble
                     guanylyl cyclase alpha2 subunit; guanylate cyclase 1,
                     soluble, alpha 2"
                     /codon_start=1
                     /product="guanylate cyclase soluble subunit alpha-2"
                     /protein_id="NP_076446.1"
                     /db_xref="GeneID:66012"
                     /db_xref="RGD:621655"
                     /translation="MSRRKISSESFSSLGSDYLETSPEEEGECPLSKLCWNGSRSPPG
                     PPGSRAAAMAATPVPAASVAAAAAAVAAGSKRAQRRRRVNLDSLGESISLLTAPSPQT
                     IHMTLKRTLQYYEHQVIGYRDAEKNFHNISNRCSSADHSNKEEIEDVSGILRCTANVL
                     GLKFQEIQERFGEEFFKICFDENERVLRAVGSTLQDFFNGFDALLEHIRTSFGKQATL
                     ESPSFLCKELPEGTLKLHYFHPHHTVGFAMLGMIKAAGKRIYHLNVEVEQIENEKFCS
                     DGSTPSNYSCLTFLIKECETTQITKNIPQGTSQIPTDLRISINTFCRTFPFHLMFDPN
                     MVVLQLGEGLRKQLRCDNHKVLKFEDCFEIVSPKVNATFDRVLLRLSTPFVIRTKPEA
                     SGTDNEDKVMEIKGQMIHVPESNAILFLGSPCVDKLDELIGRGLHLSDIPIHDATRDV
                     ILVGEQAKAQDGLKKRMDKLKATLEKTHQALEEEKKKTVDLLYSIFPGDVAQQLWQRQ
                     QVQARKFDDVTMLFSDIVGFTAICAQCTPMQVISMLNELYTRFDHQCGFLDIYKVETI
                     GDAYCVASGLHRKSLCHAKPIALMALKMMELSEEVLTPDGRPIQMRIGIHSGSVLAGV
                     VGVRMPRYCLFGNNVTLASKFESGSHPRRINISPTTYQLLKREDSFTFIPRSREELPD
                     NFPKEIPGVCYFLELRTGPKPPKPSLSSSRIKKVSYNIGTMFLRETSL"
     exon            718..779
                     /gene="Gucy1a2"
                     /inference="alignment:Splign:2.1.0"
     exon            780..901
                     /gene="Gucy1a2"
                     /inference="alignment:Splign:2.1.0"
     exon            902..1620
                     /gene="Gucy1a2"
                     /inference="alignment:Splign:2.1.0"
     exon            1621..2106
                     /gene="Gucy1a2"
                     /inference="alignment:Splign:2.1.0"
     exon            2107..2250
                     /gene="Gucy1a2"
                     /inference="alignment:Splign:2.1.0"
     exon            2251..2405
                     /gene="Gucy1a2"
                     /inference="alignment:Splign:2.1.0"
     exon            2406..2657
                     /gene="Gucy1a2"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 ggaggatcct tgctgcatgc acccttatct ccgcagtcct tcagtgggag ctttgtgcac
       61 tggccggtgc tgaggctgag cttggagaac agcagctgca gagtcagcgg tggcggtact
      121 tgaatcctca gctcatctcc ggcgccactg cggagcctgg gccgtaagga gcctcggcaa
      181 cccaagttcc cgccatgagc agctggctcg gtgggctcag ctgccccagg gacacgcatc
      241 tgggagagcg cgagcgcaac gccaagcagc cccgaagcga ctgaagctgc ctgctacagc
      301 atatccagcc acggtcacca ccaccactgc gtccctgcct ccaccaccac taccgccacc
      361 attaccacta ccaccactgc tgccgccgcc gtctctgctg cagtagtgtc tccaagcagc
      421 atgtctcgca ggaagatttc atccgagtcc ttcagctctc tgggctccga ttacctggag
      481 accagcccgg aagaggaagg ggaatgcccc ttgtctaagc tctgctggaa tggcagccgg
      541 agcccgccag ggccacctgg ctccagagca gccgctatgg ctgccacccc agtccccgct
      601 gcctctgtcg ctgcggcagc tgctgcagtc gccgccgggt ccaagagagc ccaacgccgc
      661 cggagggtca acctggactc actgggcgag agtatcagtc tcctgacggc gccctcgcct
      721 cagacgatac acatgactct caagaggaca ttgcagtatt atgaacatca agttattggt
      781 tatagggatg cagaaaagaa tttccacaat atctctaata gatgctcctc tgcagaccat
      841 tccaacaaag aggaaattga agatgtctca ggaattcttc ggtgtactgc aaatgtactc
      901 ggactgaagt tccaagaaat tcaagagagg tttggtgagg aattctttaa gatatgcttt
      961 gatgagaatg agcgagtcct tcgagctgta ggcagcacat tgcaggattt cttcaatggc
     1021 ttcgatgcat tgttggaaca cattaggact tcatttggga aacaggccac tctagagtca
     1081 ccatctttcc tatgcaaaga gcttcctgaa ggtactctca aactccacta cttccacccc
     1141 caccatacag tgggcttcgc aatgctggga atgatcaagg ctgcaggaaa gagaatctat
     1201 cacttaaatg tggaagttga acaaattgaa aatgagaaat tttgctctga tggttcaacc
     1261 ccaagcaatt atagctgtct taccttcctt atcaaagaat gtgagactac tcagatcaca
     1321 aagaacattc cacaaggtac ttcccaaatt cctacagacc tcagaatcag tatcaacacc
     1381 ttctgcagaa ccttcccatt ccatttgatg ttcgacccta acatggtggt ccttcaactt
     1441 ggagagggcc tacgaaagca acttcgatgt gacaatcaca aagtactgaa gtttgaggac
     1501 tgttttgaga tcgtctctcc aaaggttaat gccacttttg atagagtctt gctacgattg
     1561 tccaccccat ttgtgataag gacaaagcct gaagcttctg gtactgacaa tgaagataag
     1621 gtgatggaaa tcaaaggaca aatgatccat gtcccagaat caaatgccat tttgtttttg
     1681 ggctctccat gtgtggacaa gttggatgaa ctcataggcc gaggactgca tctctcagac
     1741 atccccattc atgatgccac tcgagatgtc attttagttg gagagcaagc aaaggcccag
     1801 gatggcttga aaaagaggat ggataaatta aaagccacct tagaaaaaac tcaccaggcc
     1861 ctggaagaag agaaaaagaa gacagtggat ctgctgtatt ccattttccc tggtgatgta
     1921 gcccagcagt tgtggcaacg acagcaagta caggccagaa agtttgatga tgtcactatg
     1981 ctcttctctg acattgtggg cttcacagct atatgtgccc agtgtactcc catgcaggtg
     2041 atcagcatgc tcaatgaact ttacacgaga tttgatcacc agtgtggctt tttggatatt
     2101 tacaaggtag aaacaatagg ggacgcatac tgtgttgcat cagggcttca caggaaaagc
     2161 ctatgccatg cgaagcccat tgctctgatg gccttaaaga tgatggagct ttcagaagag
     2221 gttctgactc ctgatggaag acccattcag atgcggatag gcattcattc aggctctgtg
     2281 ctagctggtg tggtcggagt gagaatgccg cgatattgtc tttttggaaa taatgtcact
     2341 ctggcaagca aatttgaatc tggaagtcat cctcggcgca tcaacatcag cccaactact
     2401 taccaattac taaaacgaga agacagtttc acatttattc ctcgttctcg tgaagaactt
     2461 ccagacaact ttccaaagga aatccctggg gtctgctatt tcctggagtt aaggactggc
     2521 ccaaagccac caaagccatc cctgtcttca tcgagaataa aaaaggtttc ctacaatatt
     2581 ggcacgatgt tcctccgaga aactagcctc tgagatttgc tgcagatcag agactcctcc
     2641 aaaaagcaca agcccag
//
