LOCUS       NM_001113570            1349 bp    mRNA    linear   ROD 29-JUL-2024
DEFINITION  Rattus norvegicus general transcription factor III A (Gtf3a), mRNA.
ACCESSION   NM_001113570 XM_001069721
VERSION     NM_001113570.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 1349)
  AUTHORS   Sloan,K.E., Bohnsack,M.T. and Watkins,N.J.
  TITLE     The 5S RNP couples p53 homeostasis to ribosome biogenesis and
            nucleolar stress
  JOURNAL   Cell Rep 5 (1), 237-247 (2013)
   PUBMED   24120868
REFERENCE   2  (bases 1 to 1349)
  AUTHORS   Vannini,A.
  TITLE     A structural perspective on RNA polymerase I and RNA polymerase III
            transcription machineries
  JOURNAL   Biochim Biophys Acta 1829 (3-4), 258-264 (2013)
   PUBMED   23031840
  REMARK    Review article
REFERENCE   3  (bases 1 to 1349)
  AUTHORS   Hanas,J.S., Hocker,J.R., Cheng,Y.G., Lerner,M.R., Brackett,D.J.,
            Lightfoot,S.A., Hanas,R.J., Madhusudhan,K.T. and Moreland,R.J.
  TITLE     cDNA cloning, DNA binding, and evolution of mammalian transcription
            factor IIIA
  JOURNAL   Gene 282 (1-2), 43-52 (2002)
   PUBMED   11814676
REFERENCE   4  (bases 1 to 1349)
  AUTHORS   Moreland,R.J., Dresser,M.E., Rodgers,J.S., Roe,B.A., Conaway,J.W.,
            Conaway,R.C. and Hanas,J.S.
  TITLE     Identification of a transcription factor IIIA-interacting protein
  JOURNAL   Nucleic Acids Res 28 (9), 1986-1993 (2000)
   PUBMED   10756201
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from CB616240.1, AF391798.1,
            CB578423.1 and CK842762.1.
            
            On Jan 25, 2008 this sequence version replaced XM_001069721.1.
            
            Summary: The product of this gene is a zinc finger protein with
            nine Cis[2]-His[2] zinc finger domains. It functions as an RNA
            polymerase III transcription factor to induce transcription of the
            5S rRNA genes. The protein binds to a 50 bp internal promoter in
            the 5S genes called the internal control region (ICR), and
            nucleates formation of a stable preinitiation complex. This complex
            recruits the TFIIIC and TFIIIB transcription factors and RNA
            polymerase III to form the complete transcription complex. The
            protein is thought to be translated using a non-AUG translation
            initiation site in mammals based on sequence analysis, protein
            homology, and the size of the purified protein. [provided by
            RefSeq, Jul 2008].
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF391798.1, SRR8487227.204770.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760383, SAMEA5760389
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            non-AUG initiation codon :: inferred from conservation
            RefSeq Select criteria   :: based on single protein-coding
                                        transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-357               CB616240.1         9-365
            358-994             AF391798.1         119-755
            995-1319            CB578423.1         48-372              c
            1320-1349           CK842762.1         1-30                c
FEATURES             Location/Qualifiers
     source          1..1349
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="Sprague-Dawley"
                     /db_xref="taxon:10116"
                     /chromosome="12"
                     /map="12p11"
     gene            1..1349
                     /gene="Gtf3a"
                     /note="general transcription factor III A"
                     /db_xref="GeneID:246299"
                     /db_xref="RGD:619746"
     exon            1..353
                     /gene="Gtf3a"
                     /inference="alignment:Splign:2.1.0"
     CDS             159..1250
                     /gene="Gtf3a"
                     /note="non-AUG (CUG) translation initiation codon; TFIIIA;
                     factor A"
                     /codon_start=1
                     /product="transcription factor IIIA"
                     /protein_id="NP_001107042.1"
                     /db_xref="GeneID:246299"
                     /db_xref="RGD:619746"
                     /translation="MEPRVSVAEAVSSLTIADAFVRACVGPAPPRPALPSRFICSFPD
                     CSASYNKAWKLDAHLCKHTGERPFVCDYEGCGKAFIRDYHLSRHILIHTGEKPFVCAD
                     NGCNQKFSTKSNLKKHIERKHENPQKQYVCNFEGCKKAFKKHQQLRTHQCQHTNEPLF
                     RCTHEGCGKHFASPSRLKRHGKVHEGYLCQKGCSFVGKTWTELLKHTREAHKEEVTCT
                     VCQKMFKRKDHLKQHMKTHAPERDVYRCPREGCARTYTTVFNLQSHILSFHEEKRPFV
                     CEHAGCGKTFAMKQSLMRHSVVHDPDKKRMKLKVRPPRERRSLASRLSGYVPPKGKQE
                     PDCSLPNSTESSSSPEATMLAPAALLTVH"
     exon            354..454
                     /gene="Gtf3a"
                     /inference="alignment:Splign:2.1.0"
     exon            455..551
                     /gene="Gtf3a"
                     /inference="alignment:Splign:2.1.0"
     exon            552..640
                     /gene="Gtf3a"
                     /inference="alignment:Splign:2.1.0"
     exon            641..714
                     /gene="Gtf3a"
                     /inference="alignment:Splign:2.1.0"
     exon            715..795
                     /gene="Gtf3a"
                     /inference="alignment:Splign:2.1.0"
     exon            796..1025
                     /gene="Gtf3a"
                     /inference="alignment:Splign:2.1.0"
     exon            1026..1085
                     /gene="Gtf3a"
                     /inference="alignment:Splign:2.1.0"
     exon            1086..1332
                     /gene="Gtf3a"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1306..1311
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Gtf3a"
     polyA_site      1332
                     /gene="Gtf3a"
ORIGIN      
        1 gcgcaatcct ggcgcatggg gtttaatggg cgggacctgg cgcctttgcg ggccgcgttc
       61 ccggaagtgt cgggtctctg ggcgccggta gcttgtgtcc cgcgttccgg ggacagcacg
      121 tggctgcccc gcctcggccc gggcgcagtt gcgtcgccct ggagccgcgg gtctcggtcg
      181 cggaagcggt gtcgtccctg accatcgcgg acgcgttcgt tcgggcctgt gtgggccccg
      241 ccccgccgcg ccccgcgctc cccagcaggt tcatctgctc ctttcctgac tgcagcgcta
      301 gttacaacaa agcctggaag ctagacgcgc acctctgcaa gcacacgggg gagaggccgt
      361 tcgtttgtga ctatgagggc tgtggcaaag ccttcatcag ggactaccat ctgagtcgac
      421 acatcctgat tcacaccgga gaaaagccat ttgtttgtgc agataatggc tgtaatcaga
      481 aattcagcac aaagtcaaac ttgaagaaac acattgaacg caaacatgaa aatccacaaa
      541 aacagtatgt gtgcaatttt gaaggttgca agaaggcctt taagaagcac cagcagctga
      601 gaacccatca gtgccagcac acaaatgagc cactcttcag gtgtacccac gagggatgtg
      661 ggaagcactt tgcctccccc agcaggctga aacggcatgg gaaggttcac gagggctacc
      721 tatgtcaaaa gggatgttcc tttgtgggaa aaacgtggac agagcttctg aagcacacga
      781 gagaagccca taaagaggag gtgacctgca cagtgtgcca gaagatgttc aagcgcaaag
      841 atcaccttaa gcagcacatg aagactcacg ctccggagag ggatgtgtac cgctgcccgc
      901 gggaaggctg tgcaaggacc tacaccaccg tgttcaacct gcagagccac attctctcct
      961 tccacgagga aaagcgccca tttgtgtgtg agcacgctgg ctgtggcaag acgtttgcaa
     1021 tgaaacaaag tctcatgagg cacagtgtcg tgcacgatcc tgacaagaag aggatgaagc
     1081 tcaaagttag accacctcgg gagagacgca gcttggcctc gcgcctcagc gggtacgtcc
     1141 ctcctaaggg gaaacaagag cccgactgct ccttgcctaa cagcacagag tccagcagca
     1201 gcccagaggc cacgatgctc gccccagctg cgttacttac tgtccactag ttgggaggga
     1261 cttcagagcg gctagcagag gcttctggag aagcccggac ttctcattaa atcactgaca
     1321 caggacgtcc gaaaaaaaaa aaaaaaaaa
//
