LOCUS       NM_022208               5993 bp    mRNA    linear   ROD 31-MAR-2024
DEFINITION  Rattus norvegicus general transcription factor 2A subunit 1
            (Gtf2a1), mRNA.
ACCESSION   NM_022208
VERSION     NM_022208.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 5993)
  AUTHORS   He,Y., Yan,C., Fang,J., Inouye,C., Tjian,R., Ivanov,I. and
            Nogales,E.
  TITLE     Near-atomic resolution visualization of human transcription
            promoter opening
  JOURNAL   Nature 533 (7603), 359-365 (2016)
   PUBMED   27193682
REFERENCE   2  (bases 1 to 5993)
  AUTHORS   Grunberg,S. and Hahn,S.
  TITLE     Structural insights into transcription initiation by RNA polymerase
            II
  JOURNAL   Trends Biochem Sci 38 (12), 603-611 (2013)
   PUBMED   24120742
  REMARK    Review article
REFERENCE   3  (bases 1 to 5993)
  AUTHORS   Anderson,D.M., Beres,B.J., Wilson-Rawls,J. and Rawls,A.
  TITLE     The homeobox gene Mohawk represses transcription by recruiting the
            sin3A/HDAC co-repressor complex
  JOURNAL   Dev Dyn 238 (3), 572-580 (2009)
   PUBMED   19235719
REFERENCE   4  (bases 1 to 5993)
  AUTHORS   Catena,R., Argentini,M., Martianov,I., Parello,C., Brancorsini,S.,
            Parvinen,M., Sassone-Corsi,P. and Davidson,I.
  TITLE     Proteolytic cleavage of ALF into alpha- and beta-subunits that form
            homologous and heterologous complexes with somatic TFIIA and TRF2
            in male germ cells
  JOURNAL   FEBS Lett 579 (16), 3401-3410 (2005)
   PUBMED   15927180
REFERENCE   5  (bases 1 to 5993)
  AUTHORS   Han,S.Y., Zhou,L., Upadhyaya,A., Lee,S.H., Parker,K.L. and
            DeJong,J.
  TITLE     TFIIAalpha/beta-like factor is encoded by a germ cell-specific gene
            whose expression is up-regulated with other general transcription
            factors during spermatogenesis in the mouse
  JOURNAL   Biol Reprod 64 (2), 507-517 (2001)
   PUBMED   11159353
REFERENCE   6  (bases 1 to 5993)
  AUTHORS   Ozer,J., Bolden,A.H. and Lieberman,P.M.
  TITLE     Transcription factor IIA mutations show activator-specific defects
            and reveal a IIA function distinct from stimulation of TBP-DNA
            binding
  JOURNAL   J Biol Chem 271 (19), 11182-11190 (1996)
   PUBMED   8626665
REFERENCE   7  (bases 1 to 5993)
  AUTHORS   DeJong,J., Bernstein,R. and Roeder,R.G.
  TITLE     Human general transcription factor TFIIA: characterization of a
            cDNA encoding the small subunit and requirement for basal and
            activated transcription
  JOURNAL   Proc Natl Acad Sci U S A 92 (8), 3313-3317 (1995)
   PUBMED   7724559
REFERENCE   8  (bases 1 to 5993)
  AUTHORS   Sun,X., Ma,D., Sheldon,M., Yeung,K. and Reinberg,D.
  TITLE     Reconstitution of human TFIIA activity from recombinant
            polypeptides: a role in TFIID-mediated transcription
  JOURNAL   Genes Dev 8 (19), 2336-2348 (1994)
   PUBMED   7958900
REFERENCE   9  (bases 1 to 5993)
  AUTHORS   Ma,D., Watanabe,H., Mermelstein,F., Admon,A., Oguri,K., Sun,X.,
            Wada,T., Imai,T., Shiroya,T., Reinberg,D. et al.
  TITLE     Isolation of a cDNA encoding the largest subunit of TFIIA reveals
            functions important for activated transcription
  JOURNAL   Genes Dev 7 (11), 2246-2257 (1993)
   PUBMED   8224850
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000006.1.
            
            On Sep 28, 2022 this sequence version replaced NM_022208.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR26360186.189795.1,
                                           SRR26643284.9174.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA5760383,
                                           SAMEA5760389 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-473               JAXUCZ010000006.1  116251748-116252220 c
            474-575             JAXUCZ010000006.1  116248996-116249097 c
            576-780             JAXUCZ010000006.1  116237801-116238005 c
            781-848             JAXUCZ010000006.1  116235226-116235293 c
            849-924             JAXUCZ010000006.1  116233124-116233199 c
            925-1058            JAXUCZ010000006.1  116231736-116231869 c
            1059-1379           JAXUCZ010000006.1  116231165-116231485 c
            1380-1469           JAXUCZ010000006.1  116227370-116227459 c
            1470-5993           JAXUCZ010000006.1  116219614-116224137 c
FEATURES             Location/Qualifiers
     source          1..5993
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="6"
                     /map="6q31"
     gene            1..5993
                     /gene="Gtf2a1"
                     /gene_synonym="Tf2a; TfIIa"
                     /note="general transcription factor 2A subunit 1"
                     /db_xref="GeneID:83830"
                     /db_xref="RGD:69246"
     exon            1..473
                     /gene="Gtf2a1"
                     /gene_synonym="Tf2a; TfIIa"
                     /inference="alignment:Splign:2.1.0"
     CDS             444..1577
                     /gene="Gtf2a1"
                     /gene_synonym="Tf2a; TfIIa"
                     /note="general transcription factor IIa, 1 (37kD and 19kD
                     subunits); general transcription factor IIA subunit 1;
                     TFIIA large subunit; general transcription factor 2a, 1"
                     /codon_start=1
                     /product="transcription initiation factor IIA subunit 1"
                     /protein_id="NP_071544.1"
                     /db_xref="GeneID:83830"
                     /db_xref="RGD:69246"
                     /translation="MANSANTNTVPKLYRSVIEDVINDVRDIFLDDGVDEQVLMELKT
                     LWENKLMQSRAVDGFHSEEQQLLLQVQQQHQPQQQQHHHHHHHQQAQPQQTVPQQAQT
                     QQVLIPASQQATAPQVIVPDSKLIQHMNASSITSAAATAATLALPAGVTPVQQILTNS
                     GQLLQVVRAANGAQYIFQPQQSVVLQQQVIPQMQPGGVQAPVIQQVLAPLPGGISPQT
                     GVIIQPQQILFTGNKTQVIPTTVAAPTPAQAQIPAAGQQQPQAQPAQQQAPLVLQVDG
                     TGDTSSEEDEDEEEDYDDDEEEDKEKDGAEDGQVEEEPLNSEDDVSDEEGQELFDTEN
                     VVVCQYDKIHRSKNKWKFHLKDGIMNLNGRDYIFSKAIGDAEW"
     exon            474..575
                     /gene="Gtf2a1"
                     /gene_synonym="Tf2a; TfIIa"
                     /inference="alignment:Splign:2.1.0"
     exon            576..780
                     /gene="Gtf2a1"
                     /gene_synonym="Tf2a; TfIIa"
                     /inference="alignment:Splign:2.1.0"
     exon            781..848
                     /gene="Gtf2a1"
                     /gene_synonym="Tf2a; TfIIa"
                     /inference="alignment:Splign:2.1.0"
     exon            849..924
                     /gene="Gtf2a1"
                     /gene_synonym="Tf2a; TfIIa"
                     /inference="alignment:Splign:2.1.0"
     exon            925..1058
                     /gene="Gtf2a1"
                     /gene_synonym="Tf2a; TfIIa"
                     /inference="alignment:Splign:2.1.0"
     exon            1059..1379
                     /gene="Gtf2a1"
                     /gene_synonym="Tf2a; TfIIa"
                     /inference="alignment:Splign:2.1.0"
     exon            1380..1469
                     /gene="Gtf2a1"
                     /gene_synonym="Tf2a; TfIIa"
                     /inference="alignment:Splign:2.1.0"
     exon            1470..5993
                     /gene="Gtf2a1"
                     /gene_synonym="Tf2a; TfIIa"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gtctctcggc ggcagcgacg gcggcggctc gcgcagcttg ttggctcgct atataaaggg
       61 gagaagcagg cggaccggac ggctggagct gcagccggtg gcggcagcgg tggcgcgggg
      121 agcggtgacc ggtgttggtt tccctcctcg gcgccgagtg ggggagcggg tgacgccccc
      181 taacccccta aggggtcgtg gcttattttt ttttcccttt tttttccttt aggcgattct
      241 cgaggttttt agctgcggga ggagtgcccc cctcctcctg gtctcccgcc tcttactcct
      301 tctggatcgg ttttgttgag aaatttttgc cccactcttg cgggtgactt gggtcaccct
      361 ccgggtctca gttttttttt ctttttcttt ttcttttttt ttttttaata tctcgttctc
      421 tcttgtttgt gttgtgtgtg gaaatggcga actcggcaaa cacaaacacc gtgcctaaat
      481 tgtatagatc tgtgattgaa gatgttatta atgatgtccg agacattttt ctggatgatg
      541 gagtggatga acaggtcctg atggaactaa aaactttgtg ggagaataaa ctaatgcagt
      601 ctagggcagt agatggattt cattcagaag aacagcagct tttgttgcaa gttcagcagc
      661 agcaccagcc ccagcagcag cagcatcacc accaccacca ccaccagcag gcgcaacctc
      721 agcaaacggt acctcagcaa gcacagaccc agcaggttct tattcctgcg tcacagcaag
      781 ctacagcacc acaagttatt gttcctgatt ctaagttgat acaacatatg aatgcatcaa
      841 gtattacgag tgctgctgct acagctgcta ccttggcgct ccctgcaggc gtgactcctg
      901 ttcagcagat actaacaaat tcaggccagc tgcttcaggt agtcagagca gccaatggtg
      961 cccaatatat ctttcagcct cagcagtcag ttgttctaca acaacaagtt ataccacaaa
     1021 tgcagcctgg tggagtacaa gcgcctgtta tccagcaggt cctagcccca cttcctggag
     1081 ggatttcacc acagaccggt gtcatcatcc agccacagca aatcttattt acagggaata
     1141 agactcaggt cattcctacg acagtggcag cgcccacacc agcacaagca cagatccctg
     1201 cagctggaca gcagcagcca caggcccagc ctgctcagca gcaggctcca ttggtgctgc
     1261 aagttgacgg aacaggggac acatcttctg aggaagatga agacgaagaa gaagactatg
     1321 atgatgatga agaggaagat aaagagaaag atggagccga agatgggcag gtggaagaag
     1381 agcccctcaa cagcgaagat gatgtaagtg atgaggaagg gcaggaactc tttgacacag
     1441 aaaatgttgt tgtatgccag tatgataaga tacacagaag taaaaataaa tggaaatttc
     1501 atctcaagga tggcattatg aatcttaatg gaagagatta tatattttcc aaagccattg
     1561 gagatgcaga gtggtgaaga gtcgcttctt tccttttata aataagacaa aagaaactta
     1621 aaacgttaac atggacggtt tgaaacttgg gacatatttc agccaaacct tcacttctgc
     1681 atgtcagaac agcgcagtag aagcaaagct acgggaacaa agctaggcct tgaccacata
     1741 gacactactt cagactggcg agccatggaa ctgtagcacc tggggctgct gggcgggggg
     1801 aggggtttgt gtaggacaac tgtaactgtc aattttaaat acaggaacct gccattggta
     1861 attagcatgt aaagctttgt ctatattcgt ttccaagttg agctcaatca gaagatacgt
     1921 gttggaatga cctgaagaaa ttaccctttt gatagattga actgaaactg cttattgtat
     1981 gtggttatat ttggtaaaaa ttgcgtgtga gctttttaca gaagggcaag aattatggtg
     2041 ttgaatttta attcatttgt ataagaaaat ttaaaactca taataggtct taaacatttt
     2101 tacttgttac tctccctcag taaaatatac ctcttgcttc tctgctgtat gaacatctac
     2161 ttaagattta cagaaaaaga tcaatggcca cagtttgaga caaagttgac ccaggagtag
     2221 atgcttggtt taattaggtt tttatacttg ttaaatgtga ttgaaagact tgggtcttac
     2281 ctgtgttatc tagaattaga gggacagttt aatttcctta cagaaaaact gctcagtccc
     2341 aggctatgag gaaaccccag attggctcct caggaatgtg tctttatttc acagaggcac
     2401 cactgagctg aaactgtata gtacacgtgc tacgcgaagg tctttcttgc cggactacag
     2461 tgtgtgctta cctgtgcatt agtctctgta aaccctggga tggcactctg tacattgatc
     2521 taagaagctt aagggcaagg catgaggact gggtttttat taaacaattt agtaattata
     2581 aaagtttctt gtgtttacta gtaaagaatt ttagaaatta ttttaattga acaaaatttt
     2641 taaaaaaact ttgtaattgg agggaaaagt ttgagggtgg agggggtaga ggaaagtggg
     2701 ccacaaaata caaatttacc tctttcattg ggtggctctt caggccattg atgagataca
     2761 tagacttgca cattggagag aaaatacaga gattagactt gtatattaaa cctgcagaca
     2821 cggccatgtg ctgagatgtg tagctctgct cccgtatggg gctctgcaga acaaagaggt
     2881 gctgcagtga gcagcttcct gggacctgca gggacactca ctctccgtca cctctgttct
     2941 agtgactaac aaaatttgtg ttcaaggtaa cttgcctgtt gacttttttt tttttttttt
     3001 ggtcttccat ttttgtcttc ctttaatttg actttatgta gttctggaag taacagataa
     3061 actatagtac aaaagaaaaa ctgtgagcta tatagtgctt ttttaagtac atattttaaa
     3121 gtttcttaaa gcagttgatt tctcctctcc acttccccct actgaaccaa tttctgttat
     3181 gaaatttgct atcagtggtg gggtcttaac gtgatagcag ttagattttg tattccataa
     3241 ctggtaatac aggttttttg tttgttcttt tttattaatg tagcttcttg aggggaagtc
     3301 tgtttaatct cctattctca aagcacaccg cagttctggt tgaactaaac cttggtgaac
     3361 agagggcaca ggatcccgag agttgttgag tggtcccttg ctatagtttc taggtgtgtg
     3421 tttactcagt gaatcagtaa cctgaactac ctacagcagc acagcagtgt ggccgcagtg
     3481 ggactagtgg gctatgttag tgccgcacat gccctggctt ctctgtgtag tcagacatac
     3541 tgaaggttag cattgaacac tggcctgttt tctagccaaa atgttcagac gtgagcaacc
     3601 acttctttga gagtgaagca ggaactgacc attgatattt gtatctatgt gtactgacta
     3661 ataatgctgg catcctggac aatactgtga tctgggaaag ctctgaaaac tggttccagc
     3721 cgccctgcac accagactgt gcttttccat gctgttatat tgtgctcctt ttaaatcaag
     3781 tagcatgctg cattcctcag gatagtagct tttgctgtgt aatgtcactt gctttgaatt
     3841 ttcaatcatg ctttcgccat tacatttaac caatgatgtc attaagttct attaaagtgt
     3901 cattcctttg ggaagcaagg gattcccact tgcagaattt taagatgaag gtagcatgtt
     3961 ttgttcagaa ggtggaaata gggactcgaa cagtgtcttc agaagctgat ctgttgagat
     4021 ttgtgtgcac agtgaaggca cctcttacat gatgtgaggc cagtagcgct aaggtaaatt
     4081 tacacagatg actaaggtgg gatgtgtgcc ttccagtcca tgaactcttg cctggtggtc
     4141 gtttgtattc tcacaccacc aaaatgcccg tgagcacatg ggatttccta gagtaataca
     4201 actttgtcac gtgcagtttc tagagtcaaa caggatagga gggggtcttt atattaggct
     4261 tttatatctg aaatgttcac atgtctatta aatgtgtcac acttacactg ttgccttttg
     4321 aaaagtcagg ctggcatcaa gattcatgca gtacatatag ttatcaaagt gtgttattga
     4381 caagcattct ggctttagaa ggcaatgtaa aaaaatacta atttttatat aaaggctttc
     4441 ttttgctgta ttgaaaatca ggtttttctt ttggtgactt gtatggatga atgcagagtt
     4501 gtctccctct gagaatattt tttttattat cttatagcaa taatgtcacc atattagagg
     4561 gtaagtattc cttgaataag aaaacacaca tatctcctct tagcagtttt ccctttataa
     4621 cagcattttc ttccacttga gaaggctctt atattttcat attacttcat cttgaacttt
     4681 tgagcttttg tgcaatgtgc tattttattg gatctttgta cgttttcatt tcatgtttcc
     4741 tccctacctg tctgaagctg ctatagctga tgttggggtt tcggttaatg gttatgtgtt
     4801 ttctcatctt tattaaagtt aggtttatct tttaatttgt tacccttatg gaaattattc
     4861 tggtttatac tcttccccct ggaagcctgc ctttgtccat ctgttatctt actttcgtgc
     4921 atgatttaag gaagattgca tcaaatttaa tatttttatt ttgaagaaga aaagcttcct
     4981 accatttgag gggagaacaa aacaacattg aataaagaat tggtcgttca gtcaaacttc
     5041 cttaagtcag ttcagaatgc ctcggttaca ccgatggggg ctcgtcccta gctggtcaga
     5101 gttactgttg tcgactgctg ttgtacatac tgttatgtgt aagggcgtaa atatatttat
     5161 tatgtttgta aaattcattc tgtacacttg cagatcaagg ttgctcttct gtgatatatc
     5221 gggtgttacg ttttaaagac catcttggaa tacaactaaa gagcttagta ttttgatgta
     5281 ctaagactat tttaagttta atattttacc ttgcaaaaac tttaattaaa gatgttattt
     5341 aaaaaaagta atgtggcttg ctttgcttac tagtcagtga cgctggtata tggacaactg
     5401 agaaaactaa aaccatgcag tttagacagg aagttcaaca aaaacgggga tatcgttaaa
     5461 ttttgtaccc tcggttaaat ctgtacctgt aaggtagtgg gtttgggatg ccagactgaa
     5521 ttgttaaact gtgttagaat tccttaagcg ctttcaaaag ttgtattttc caggcacaga
     5581 agcatttaga atgggtaaat tcacaccaag taaatgtaaa ttaaggttaa tataattaac
     5641 attgtattaa atgagtattt ctgctcacga agcttagatt ccaccagatg aagttattaa
     5701 ggacatcgtt ttgtttgttt ggctaaactg tctctcagtt cggagtttgt aaataatcgc
     5761 tcactaggag attgggttat tgaggtttgt attacatagg aatatatttt gtgttacttt
     5821 acaagatatt aaaattacta gcagtatttt gattttatag ttaattcaaa tggatcatta
     5881 agagcttgcc tttttgtaat tttttatcct gttataaact actttatcta aggaaacatg
     5941 cagattttaa ctattaaata atcataataa agatatttta tttattgcca gca
//
