LOCUS       NP_001106183             576 aa            linear   ROD 31-MAR-2024
DEFINITION  G protein-coupled receptor kinase 6 isoform c [Rattus norvegicus].
ACCESSION   NP_001106183
VERSION     NP_001106183.1
DBSOURCE    REFSEQ: accession NM_001112712.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (residues 1 to 576)
  AUTHORS   Tian,Y.Q., Li,J.H., Li,Y.C., Xu,Y.C., Zhang,P.A., Wang,Q., Li,R.
            and Xu,G.Y.
  TITLE     Overexpression of GRK6 alleviates chronic visceral hypersensitivity
            through downregulation of P2Y6 receptors in anterior cingulate
            cortex of rats with prenatal maternal stress
  JOURNAL   CNS Neurosci Ther 28 (6), 851-861 (2022)
   PUBMED   35349212
  REMARK    GeneRIF: Overexpression of GRK6 alleviates chronic visceral
            hypersensitivity through downregulation of P2Y6 receptors in
            anterior cingulate cortex of rats with prenatal maternal stress.
REFERENCE   2  (residues 1 to 576)
  AUTHORS   Li,X., Xu,Y.C., Tian,Y.Q., Zhang,P.A., Hu,S.F., Wang,L.H.,
            Jiang,X.H. and Xu,G.Y.
  TITLE     Downregulation of GRK6 in arcuate nucleus promotes chronic visceral
            hypersensitivity via NF-kappaB upregulation in adult rats with
            neonatal maternal deprivation
  JOURNAL   Mol Pain 16, 1744806920930858 (2020)
   PUBMED   32484026
  REMARK    GeneRIF: Downregulation of GRK6 in arcuate nucleus promotes chronic
            visceral hypersensitivity via NF-kappaB upregulation in adult rats
            with neonatal maternal deprivation.
REFERENCE   3  (residues 1 to 576)
  AUTHORS   Wang,L., Bao,H., Wang,K.X., Zhang,P., Yao,Q.P., Chen,X.H.,
            Huang,K., Qi,Y.X. and Jiang,Z.L.
  TITLE     Secreted miR-27a Induced by Cyclic Stretch Modulates the
            Proliferation of Endothelial Cells in Hypertension via GRK6
  JOURNAL   Sci Rep 7, 41058 (2017)
   PUBMED   28106155
  REMARK    GeneRIF: Study shows that secreted miR-27a induced by cyclic
            stretch modulates the proliferation of endothelial cells in
            hypertension via g-protein-coupled receptor kinase 6.
            Publication Status: Online-Only
REFERENCE   4  (residues 1 to 576)
  AUTHORS   Zhou,Y., Li,R.J., Li,M., Liu,X., Zhu,H.Y., Ju,Z., Miao,X. and
            Xu,G.Y.
  TITLE     Overexpression of GRK6 attenuates neuropathic pain via suppression
            of CXCR2 in rat dorsal root ganglion
  JOURNAL   Mol Pain 12, 1744806916646381 (2016)
   PUBMED   27145805
  REMARK    GeneRIF: GRK6 might be a key molecular involved in peripheral
            mechanism of neuropathic pain and that overexpression of GRK6 might
            be a potential strategy for treatment for neuropathic pain through
            inhibition of CXCR2 signal pathway.
            Publication Status: Online-Only
REFERENCE   5  (residues 1 to 576)
  AUTHORS   Oda,Y., Tadokoro,S., Takase,M., Kanahara,N., Watanabe,H.,
            Shirayama,Y., Hashimoto,K. and Iyo,M.
  TITLE     G protein-coupled receptor kinase 6/beta-arrestin 2 system in a rat
            model of dopamine supersensitivity psychosis
  JOURNAL   J Psychopharmacol 29 (12), 1308-1313 (2015)
   PUBMED   26174132
  REMARK    GeneRIF: The ratio of GRK6/ARRB2 in DSP model rats was
            significantly higher than that in controls.
REFERENCE   6  (residues 1 to 576)
  AUTHORS   Premont,R.T., Macrae,A.D., Aparicio,S.A., Kendall,H.E., Welch,J.E.
            and Lefkowitz,R.J.
  TITLE     The GRK4 subfamily of G protein-coupled receptor kinases.
            Alternative splicing, gene organization, and sequence conservation
  JOURNAL   J Biol Chem 274 (41), 29381-29389 (1999)
   PUBMED   10506199
REFERENCE   7  (residues 1 to 576)
  AUTHORS   Hall,R.A., Spurney,R.F., Premont,R.T., Rahman,N., Blitzer,J.T.,
            Pitcher,J.A. and Lefkowitz,R.J.
  TITLE     G protein-coupled receptor kinase 6A phosphorylates the Na(+)/H(+)
            exchanger regulatory factor via a PDZ domain-mediated interaction
  JOURNAL   J Biol Chem 274 (34), 24328-24334 (1999)
   PUBMED   10446210
REFERENCE   8  (residues 1 to 576)
  AUTHORS   Lombardi,M.S., Kavelaars,A., Schedlowski,M., Bijlsma,J.W.,
            Okihara,K.L., Van de Pol,M., Ochsmann,S., Pawlak,C., Schmidt,R.E.
            and Heijnen,C.J.
  TITLE     Decreased expression and activity of G-protein-coupled receptor
            kinases in peripheral blood mononuclear cells of patients with
            rheumatoid arthritis
  JOURNAL   FASEB J 13 (6), 715-725 (1999)
   PUBMED   10094932
REFERENCE   9  (residues 1 to 576)
  AUTHORS   Fehr,C., Fickova,M., Hiemke,C., Reuss,S. and Dahmen,N.
  TITLE     Molecular cloning of rat G-protein-coupled receptor kinase 6 (GRK6)
            from brain tissue, and its mRNA expression in different brain
            regions and peripheral tissues
  JOURNAL   Brain Res Mol Brain Res 49 (1-2), 278-282 (1997)
   PUBMED   9387888
REFERENCE   10 (residues 1 to 576)
  AUTHORS   Firsov,D. and Elalouf,J.M.
  TITLE     Molecular cloning of two rat GRK6 splice variants
  JOURNAL   Am J Physiol 273 (3 Pt 1), C953-C961 (1997)
   PUBMED   9316417
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000017.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: X97439.1, SRR26643298.68431.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA5760383,
                                           SAMEA5760386 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..576
                     /organism="Rattus norvegicus"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="17"
                     /map="17p14"
     Protein         1..576
                     /product="G protein-coupled receptor kinase 6 isoform c"
                     /EC_number="2.7.11.16"
                     /note="g protein-coupled receptor kinase GRK6"
                     /calculated_mol_wt=65871
     Region          1..185
                     /region_name="N-terminal"
                     /note="propagated from UniProtKB/Swiss-Prot (P97711.1)"
     Region          35..179
                     /region_name="RGS"
                     /note="Regulator of G protein signaling (RGS) domain
                     superfamily; cl02565"
                     /db_xref="CDD:470619"
     Region          185..469
                     /region_name="STKc_GRK6"
                     /note="Catalytic domain of the Serine/Threonine Kinase, G
                     protein-coupled Receptor Kinase 6; cd05630"
                     /db_xref="CDD:270779"
     Site            order(192..196,200,213,215,247,263..264,266,270,272,311,
                     313,315..316,318,328..329,332,345..350,377,383,386)
                     /site_type="active"
                     /db_xref="CDD:270779"
     Site            order(192..196,200,213,215,264..266,318,329)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270779"
     Site            order(196,270,272,311,313,315,332,345..350,377,383,386)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:270779"
     Site            328..350
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:270779"
     Site            484
                     /site_type="phosphorylation"
                     /note="Phosphoserine, by autocatalysis.
                     /evidence=ECO:0000250|UniProtKB:P43250; propagated from
                     UniProtKB/Swiss-Prot (P97711.1)"
     Site            485
                     /site_type="phosphorylation"
                     /note="Phosphothreonine, by autocatalysis.
                     /evidence=ECO:0000250|UniProtKB:P43250; propagated from
                     UniProtKB/Swiss-Prot (P97711.1)"
     Site            566
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:O70293; propagated from
                     UniProtKB/Swiss-Prot (P97711.1)"
     Site            568
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:O70293; propagated from
                     UniProtKB/Swiss-Prot (P97711.1)"
     CDS             1..576
                     /gene="Grk6"
                     /gene_synonym="Gprk6"
                     /coded_by="NM_001112712.2:178..1908"
                     /note="isoform c is encoded by transcript variant 3"
                     /db_xref="GeneID:59076"
                     /db_xref="RGD:61986"
ORIGIN      
        1 melenivant vllkareggg gnrkgkskkw rqmlqfphis qceelrlsle rdyhslcerq
       61 pigrllfref catrpeltrc tafldgvaey evtpdekrka cgrrlmqnfl shtgpdlipe
      121 vprqlvsnca qrleqgpckd lfqeltrlth eylsmapfad yldsiyfnrf lqwkwlerqp
      181 vtkntfrqyr vlgkggfgev cacqvratgk myackklekk rikkrkgeam alnekqilek
      241 vnsrfvvsla yayetkdalc lvltlmnggd lkfhiyhmgq agfpearavf yaaeiccgle
      301 dlhrerivyr dlkpenilld dhghirisdl glavhvpegq tikgrvgtvg ymapevvkne
      361 rytfspdwwa lgcllyemia gqspfqqrkk kikreeverl vkevaeeytd rfspqarslc
      421 sqllnkdpae rlgcrgggar evkehplfkk lnfkrlgagm leppfkpdpq aiyckdvldi
      481 eqfstvkgvd leptdqdfyq kfatgsvsip wqnemvetec fqelnvfgld gsvppdldwk
      541 gqptappkkg llqrlfsrqd ccgncsdsee elptrl
//
