LOCUS       NP_075217                575 aa            linear   ROD 05-MAY-2025
DEFINITION  G protein-coupled receptor kinase 4 isoform 1 [Rattus norvegicus].
ACCESSION   NP_075217
VERSION     NP_075217.1
DBSOURCE    REFSEQ: accession NM_022928.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (residues 1 to 575)
  AUTHORS   Zhang,Y., Wang,S., Huang,H., Zeng,A., Han,Y., Zeng,C., Zheng,S.,
            Ren,H., Wang,Y., Huang,Y., Jose,P.A., Ma,X.L., Zeng,C. and Chen,K.
  TITLE     GRK4-mediated adiponectin receptor-1 phosphorylative
            desensitization as a novel mechanism of reduced renal sodium
            excretion in hypertension
  JOURNAL   Clin Sci (Lond) 134 (18), 2453-2467 (2020)
   PUBMED   32940654
  REMARK    GeneRIF: GRK4-mediated adiponectin receptor-1 phosphorylative
            desensitization as a novel mechanism of reduced renal sodium
            excretion in hypertension.
REFERENCE   2  (residues 1 to 575)
  AUTHORS   Yang,Y., Li,M., Zou,X., Chen,C., Zheng,S., Fu,C., Chen,K.,
            Jose,P.A., Lan,C. and Liu,Y.
  TITLE     Role of GRK4 in the regulation of the renal ETB receptor in
            hypertension
  JOURNAL   FASEB J 34 (9), 11594-11604 (2020)
   PUBMED   32687659
  REMARK    GeneRIF: Role of GRK4 in the regulation of the renal ETB receptor
            in hypertension.
            Erratum:[FASEB J. 2021 Apr;35(4):e21429. doi: 10.1096/fsb2.21429.
            PMID: 33734484]
REFERENCE   3  (residues 1 to 575)
  AUTHORS   Chougule,R.A., P,S., Salimath,B.P. and Sosalegowda,A.H.
  TITLE     Buffalo colostrum beta-lactoglobulin inhibits VEGF-induced
            angiogenesis by interacting with G protein-coupled receptor kinase
  JOURNAL   Appl Biochem Biotechnol 171 (2), 366-381 (2013)
   PUBMED   23839509
  REMARK    GeneRIF: Buffalo colostrum beta-lactoglobulin inhibits VEGF-induced
            angiogenesis by interacting with G protein-coupled receptor kinase
REFERENCE   4  (residues 1 to 575)
  AUTHORS   Wang,F.L., Tang,L.Q., Yang,F., Zhu,L.N., Cai,M. and Wei,W.
  TITLE     Renoprotective effects of berberine and its possible molecular
            mechanisms in combination of high-fat diet and low-dose
            streptozotocin-induced diabetic rats
  JOURNAL   Mol Biol Rep 40 (3), 2405-2418 (2013)
   PUBMED   23196710
REFERENCE   5  (residues 1 to 575)
  AUTHORS   Kuwasako,K., Kitamura,K., Nagata,S., Hikosaka,T. and Kato,J.
  TITLE     Function of the cytoplasmic tail of human calcitonin receptor-like
            receptor in complex with receptor activity-modifying protein 2
  JOURNAL   Biochem Biophys Res Commun 392 (3), 380-385 (2010)
   PUBMED   20074556
REFERENCE   6  (residues 1 to 575)
  AUTHORS   Speirs,H.J., Katyk,K., Kumar,N.N., Benjafield,A.V., Wang,W.Y. and
            Morris,B.J.
  TITLE     Association of G-protein-coupled receptor kinase 4 haplotypes, but
            not HSD3B1 or PTP1B polymorphisms, with essential hypertension
  JOURNAL   J Hypertens 22 (5), 931-936 (2004)
   PUBMED   15097232
REFERENCE   7  (residues 1 to 575)
  AUTHORS   Iacovelli,L., Salvatore,L., Capobianco,L., Picascia,A.,
            Barletta,E., Storto,M., Mariggio,S., Sallese,M., Porcellini,A.,
            Nicoletti,F. and De Blasi,A.
  TITLE     Role of G protein-coupled receptor kinase 4 and beta-arrestin 1 in
            agonist-stimulated metabotropic glutamate receptor 1
            internalization and activation of mitogen-activated protein kinases
  JOURNAL   J Biol Chem 278 (14), 12433-12442 (2003)
   PUBMED   12519791
REFERENCE   8  (residues 1 to 575)
  AUTHORS   Sallese,M., Salvatore,L., D'Urbano,E., Sala,G., Storto,M.,
            Launey,T., Nicoletti,F., Knopfel,T. and De Blasi,A.
  TITLE     The G-protein-coupled receptor kinase GRK4 mediates homologous
            desensitization of metabotropic glutamate receptor 1
  JOURNAL   FASEB J 14 (15), 2569-2580 (2000)
   PUBMED   11099476
REFERENCE   9  (residues 1 to 575)
  AUTHORS   Premont,R.T., Macrae,A.D., Aparicio,S.A., Kendall,H.E., Welch,J.E.
            and Lefkowitz,R.J.
  TITLE     The GRK4 subfamily of G protein-coupled receptor kinases.
            Alternative splicing, gene organization, and sequence conservation
  JOURNAL   J Biol Chem 274 (41), 29381-29389 (1999)
   PUBMED   10506199
REFERENCE   10 (residues 1 to 575)
  AUTHORS   Virlon,B., Firsov,D., Cheval,L., Reiter,E., Troispoux,C.,
            Guillou,F. and Elalouf,J.M.
  TITLE     Rat G protein-coupled receptor kinase GRK4: identification,
            functional expression, and differential tissue distribution of two
            splice variants
  JOURNAL   Endocrinology 139 (6), 2784-2795 (1998)
   PUBMED   9607785
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000014.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: X97568.1, SRR26643293.57412.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA5760383,
                                           SAMEA5760386 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on expression
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..575
                     /organism="Rattus norvegicus"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="14"
                     /map="14q21"
     Protein         1..575
                     /product="G protein-coupled receptor kinase 4 isoform 1"
                     /EC_number="2.7.11.16"
                     /note="g protein-coupled receptor kinase GRK4; G
                     protein-coupled receptor kinase 2, groucho gene related"
                     /calculated_mol_wt=66657
     Region          1..153
                     /region_name="N-terminal"
                     /note="propagated from UniProtKB/Swiss-Prot (P70507.1)"
     Site            1
                     /site_type="acetylation"
                     /note="N-acetylmethionine.
                     /evidence=ECO:0000250|UniProtKB:P32298; propagated from
                     UniProtKB/Swiss-Prot (P70507.1)"
     Region          51..183
                     /region_name="RGS"
                     /note="Regulator of G protein signaling (RGS) domain
                     superfamily; cl02565"
                     /db_xref="CDD:470619"
     Region          185..469
                     /region_name="STKc_GRK4_like"
                     /note="Catalytic domain of G protein-coupled Receptor
                     Kinase 4-like Serine/Threonine Kinases; cd05605"
                     /db_xref="CDD:270756"
     Site            order(192..196,200,213,215,247,263..264,266,270,272,311,
                     313,315..316,318,328..329,332,345..350,377,383,386)
                     /site_type="active"
                     /db_xref="CDD:270756"
     Site            order(192..196,200,213,215,264..266,318,329)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270756"
     Site            order(196,270,272,311,313,315,332,345..350,377,383,386)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:270756"
     Site            328..350
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:270756"
     Site            484
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0007744|PubMed:22673903; propagated from
                     UniProtKB/Swiss-Prot (P70507.1)"
     CDS             1..575
                     /gene="Grk4"
                     /gene_synonym="Gprk2l"
                     /coded_by="NM_022928.2:558..2285"
                     /note="isoform 1 is encoded by transcript variant 1"
                     /db_xref="GeneID:59077"
                     /db_xref="RGD:61858"
ORIGIN      
        1 melenfmant lllkarqgft kktgrskkwr ellklppvsm csdlrhsiek dfsslcdqqp
       61 igrllfrqfc ntkpdlkrci efldaaaeye vtieeeqref glsiysrffk ensevslpqi
      121 ppdlvkeckc nlkqsspsqn vfqdcagviy kylsekpfee yqestyynrf lqwkwlerrp
      181 vtkntfrqyr vlgkggfgev cacqvratgk myackklekk rikkrkgeam alnekrilek
      241 lhsrfvvsla ytyetkdalc lvltimnggd lkyhiynlgn pgfeeqravf yaaelccgle
      301 dlqrerivyr dlkpenilld dhghirisdl glaleipege mvrgrvgtvg ymapeiishe
      361 kytfspdwwg lgcliyemia ghspfrkyke kvnreemerr vkteteeyse rfsenaksic
      421 smlltkdpsk rlgcqsdgas avkqhpifkd infsrleanm ldppfcpdpe aiyckdildi
      481 gqfsvvkgvn ldtndeifyt qfatgcvtip wqnemiesgc fkdlneyedk glsplekhki
      541 cscilrpkrn ffhrlfrraa clniahseer epteh
//
