LOCUS       NM_012776               3327 bp    mRNA    linear   ROD 31-MAR-2024
DEFINITION  Rattus norvegicus G protein-coupled receptor kinase 2 (Grk2), mRNA.
ACCESSION   NM_012776 XM_346576
VERSION     NM_012776.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 3327)
  AUTHORS   Alonazi,A., Nash,C.A., Wang,C.H., Christofidou,E., Challiss,R.A.J.
            and Willets,J.M.
  TITLE     GRK2 expression and catalytic activity are essential for
            vasoconstrictor/ERK-stimulated arterial smooth muscle proliferation
  JOURNAL   Biochem Pharmacol 216, 115795 (2023)
   PUBMED   37690571
  REMARK    GeneRIF: GRK2 expression and catalytic activity are essential for
            vasoconstrictor/ERK-stimulated arterial smooth muscle
            proliferation.
REFERENCE   2  (bases 1 to 3327)
  AUTHORS   Liu,J., Li,X., Ding,L., Li,W., Niu,X. and Gao,D.
  TITLE     GRK2 participation in cardiac hypertrophy induced by isoproterenol
            through the regulation of Nrf2 signaling and the promotion of NLRP3
            inflammasome and oxidative stress
  JOURNAL   Int Immunopharmacol 117, 109957 (2023)
   PUBMED   37012864
  REMARK    GeneRIF: GRK2 participation in cardiac hypertrophy induced by
            isoproterenol through the regulation of Nrf2 signaling and the
            promotion of NLRP3 inflammasome and oxidative stress.
REFERENCE   3  (bases 1 to 3327)
  AUTHORS   Xu,X.H., Du,R.Q., Li,L., Yang,L.L., Zhang,Y. and Li,Q.M.
  TITLE     The role of G-protein-coupled receptor kinase 2 in diabetic
            mechanical hyperalgesia in rats
  JOURNAL   Eur J Pain 25 (9), 2039-2049 (2021)
   PUBMED   34101933
  REMARK    GeneRIF: The role of G-protein-coupled receptor kinase 2 in
            diabetic mechanical hyperalgesia in rats.
REFERENCE   4  (bases 1 to 3327)
  AUTHORS   Hu,S., Guo,P., Wang,Z., Zhou,Z., Wang,R., Zhang,M., Tao,J., Tai,Y.,
            Zhou,W., Wei,W. and Wang,Q.
  TITLE     Down-regulation of A3AR signaling by IL-6-induced GRK2 activation
            contributes to Th17 cell differentiation
  JOURNAL   Exp Cell Res 399 (2), 112482 (2021)
   PUBMED   33434531
  REMARK    GeneRIF: Down-regulation of A3AR signaling by IL-6-induced GRK2
            activation contributes to Th17 cell differentiation.
REFERENCE   5  (bases 1 to 3327)
  AUTHORS   Penela,P., Inserte,J., Ramos,P., Rodriguez-Sinovas,A.,
            Garcia-Dorado,D. and Mayor,F. Jr.
  TITLE     Degradation of GRK2 and AKT is an early and detrimental event in
            myocardial ischemia/reperfusion
  JOURNAL   EBioMedicine 48, 605-618 (2019)
   PUBMED   31594751
  REMARK    GeneRIF: Preventing transient degradation of GRK2 and AKT during
            early I/R might improve the potential of endogenous
            cardioprotection mechanisms and of conditioning strategies.
REFERENCE   6  (bases 1 to 3327)
  AUTHORS   Jaber,M., Koch,W.J., Rockman,H., Smith,B., Bond,R.A., Sulik,K.K.,
            Ross,J. Jr., Lefkowitz,R.J., Caron,M.G. and Giros,B.
  TITLE     Essential role of beta-adrenergic receptor kinase 1 in cardiac
            development and function
  JOURNAL   Proc Natl Acad Sci U S A 93 (23), 12974-12979 (1996)
   PUBMED   8917529
REFERENCE   7  (bases 1 to 3327)
  AUTHORS   Koch,W.J., Rockman,H.A., Samama,P., Hamilton,R.A., Bond,R.A.,
            Milano,C.A. and Lefkowitz,R.J.
  TITLE     Cardiac function in mice overexpressing the beta-adrenergic
            receptor kinase or a beta ARK inhibitor
  JOURNAL   Science 268 (5215), 1350-1353 (1995)
   PUBMED   7761854
REFERENCE   8  (bases 1 to 3327)
  AUTHORS   Arriza,J.L., Dawson,T.M., Simerly,R.B., Martin,L.J., Caron,M.G.,
            Snyder,S.H. and Lefkowitz,R.J.
  TITLE     The G-protein-coupled receptor kinases beta ARK1 and beta ARK2 are
            widely distributed at synapses in rat brain
  JOURNAL   J Neurosci 12 (10), 4045-4055 (1992)
   PUBMED   1403099
REFERENCE   9  (bases 1 to 3327)
  AUTHORS   Owada,Y., Watanabe,M. and Kondo,H.
  TITLE     Localization of mRNA for beta-adrenergic receptor kinase in the
            brain of adult rats
  JOURNAL   Neurosci Lett 144 (1-2), 9-13 (1992)
   PUBMED   1436718
REFERENCE   10 (bases 1 to 3327)
  AUTHORS   Benovic,J.L., Onorato,J.J., Arriza,J.L., Stone,W.C., Lohse,M.,
            Jenkins,N.A., Gilbert,D.J., Copeland,N.G., Caron,M.G. and
            Lefkowitz,R.J.
  TITLE     Cloning, expression, and chromosomal localization of
            beta-adrenergic receptor kinase 2. A new member of the receptor
            kinase family
  JOURNAL   J Biol Chem 266 (23), 14939-14946 (1991)
   PUBMED   1869533
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000001.1.
            
            On Feb 26, 2021 this sequence version replaced NM_012776.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: M87854.1, SRR26643429.4400.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA5760393,
                                           SAMEA5760400 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-248               JAXUCZ010000001.1  211030766-211031013 c
            249-325             JAXUCZ010000001.1  211018946-211019022 c
            326-399             JAXUCZ010000001.1  211016970-211017043 c
            400-501             JAXUCZ010000001.1  211016746-211016847 c
            502-576             JAXUCZ010000001.1  211016552-211016626 c
            577-638             JAXUCZ010000001.1  211016349-211016410 c
            639-690             JAXUCZ010000001.1  211015774-211015825 c
            691-782             JAXUCZ010000001.1  211015419-211015510 c
            783-882             JAXUCZ010000001.1  211014954-211015053 c
            883-961             JAXUCZ010000001.1  211014791-211014869 c
            962-1092            JAXUCZ010000001.1  211014575-211014705 c
            1093-1187           JAXUCZ010000001.1  211014239-211014333 c
            1188-1295           JAXUCZ010000001.1  211014060-211014167 c
            1296-1362           JAXUCZ010000001.1  211013822-211013888 c
            1363-1463           JAXUCZ010000001.1  211013430-211013530 c
            1464-1530           JAXUCZ010000001.1  211012708-211012774 c
            1531-1626           JAXUCZ010000001.1  211012533-211012628 c
            1627-1789           JAXUCZ010000001.1  211012112-211012274 c
            1790-1926           JAXUCZ010000001.1  211011828-211011964 c
            1927-2040           JAXUCZ010000001.1  211011625-211011738 c
            2041-3327           JAXUCZ010000001.1  211010259-211011545 c
FEATURES             Location/Qualifiers
     source          1..3327
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="1"
                     /map="1q43"
     gene            1..3327
                     /gene="Grk2"
                     /gene_synonym="Adrbk1; BARK1; GRK-2"
                     /note="G protein-coupled receptor kinase 2"
                     /db_xref="GeneID:25238"
                     /db_xref="RGD:2062"
     exon            1..248
                     /gene="Grk2"
                     /gene_synonym="Adrbk1; BARK1; GRK-2"
                     /inference="alignment:Splign:2.1.0"
     CDS             136..2205
                     /gene="Grk2"
                     /gene_synonym="Adrbk1; BARK1; GRK-2"
                     /EC_number="2.7.11.15"
                     /note="beta-ARK-1; adrenergic receptor kinase, beta 1;
                     beta-adrenergic receptor kinase 1 beta ARK1; adrenergic,
                     beta, receptor kinase 1"
                     /codon_start=1
                     /product="beta-adrenergic receptor kinase 1"
                     /protein_id="NP_036908.2"
                     /db_xref="GeneID:25238"
                     /db_xref="RGD:2062"
                     /translation="MADLEAVLADVSYLMAMEKSKATPAARASKKILLPEPSIRSVMQ
                     KYLEDRGEVTFEKIFSQKLGYLLFRDFCLNHLEEAKPLVEFYEEIKKYEKLETEEERV
                     VRSREIFDSYIMKELLACSHPFSKNATEHVQGHLVKKQVPPDLFQPYIEEICQNLRGD
                     VFHKFIESDKFTRFCQWKNVELNIHLTMNDFSVHRIIGRGGFGEVYGCRKADTGKMYA
                     MKCLDKKRIKMKQGETLALNERIMLSLVSTGDCPFIVCMSYAFHTPDKLSFILDLMNG
                     GDLHYHLSQHGVFSEADMRFYAAEIILGLEHMHNRFVVYRDLKPANILLDEHGHVRIS
                     DLGLACDFSKKKPHASVGTHGYMAPEVLQKGVAYDSSADWFSLGCMLFKLLRGHSPFR
                     QHKTKDKHEIDRMTLTMAVELPDSFSPELRSLLEGLLQRDVNRRLGCLGRGAQEVKES
                     PFFRSLDWQMVFLQKYPPPLIPPRGEVNAADAFDIGSFDEEDTKGIKLLDSDQELYRN
                     FPLTISERWQQEVAETVFDTINAETDRLEARKKAKNKQLGHEEDYALGKDCIMHGYMS
                     KMGNPFLTQWQRRYFYLFPNRLEWRGEGEAPQSLLTMEEIQSVEETQIKERKCLLLKI
                     RGGKQFVLQCDSDPELVQWKKELRDAYREAQQLVQRVPKMKNKPRSPVVELSKVPLIQ
                     RGSANGL"
     exon            249..325
                     /gene="Grk2"
                     /gene_synonym="Adrbk1; BARK1; GRK-2"
                     /inference="alignment:Splign:2.1.0"
     exon            326..399
                     /gene="Grk2"
                     /gene_synonym="Adrbk1; BARK1; GRK-2"
                     /inference="alignment:Splign:2.1.0"
     exon            400..501
                     /gene="Grk2"
                     /gene_synonym="Adrbk1; BARK1; GRK-2"
                     /inference="alignment:Splign:2.1.0"
     exon            502..576
                     /gene="Grk2"
                     /gene_synonym="Adrbk1; BARK1; GRK-2"
                     /inference="alignment:Splign:2.1.0"
     exon            577..638
                     /gene="Grk2"
                     /gene_synonym="Adrbk1; BARK1; GRK-2"
                     /inference="alignment:Splign:2.1.0"
     exon            639..690
                     /gene="Grk2"
                     /gene_synonym="Adrbk1; BARK1; GRK-2"
                     /inference="alignment:Splign:2.1.0"
     exon            691..782
                     /gene="Grk2"
                     /gene_synonym="Adrbk1; BARK1; GRK-2"
                     /inference="alignment:Splign:2.1.0"
     exon            783..882
                     /gene="Grk2"
                     /gene_synonym="Adrbk1; BARK1; GRK-2"
                     /inference="alignment:Splign:2.1.0"
     exon            883..961
                     /gene="Grk2"
                     /gene_synonym="Adrbk1; BARK1; GRK-2"
                     /inference="alignment:Splign:2.1.0"
     exon            962..1092
                     /gene="Grk2"
                     /gene_synonym="Adrbk1; BARK1; GRK-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1093..1187
                     /gene="Grk2"
                     /gene_synonym="Adrbk1; BARK1; GRK-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1188..1295
                     /gene="Grk2"
                     /gene_synonym="Adrbk1; BARK1; GRK-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1296..1362
                     /gene="Grk2"
                     /gene_synonym="Adrbk1; BARK1; GRK-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1363..1463
                     /gene="Grk2"
                     /gene_synonym="Adrbk1; BARK1; GRK-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1464..1530
                     /gene="Grk2"
                     /gene_synonym="Adrbk1; BARK1; GRK-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1531..1626
                     /gene="Grk2"
                     /gene_synonym="Adrbk1; BARK1; GRK-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1627..1789
                     /gene="Grk2"
                     /gene_synonym="Adrbk1; BARK1; GRK-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1790..1926
                     /gene="Grk2"
                     /gene_synonym="Adrbk1; BARK1; GRK-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1927..2040
                     /gene="Grk2"
                     /gene_synonym="Adrbk1; BARK1; GRK-2"
                     /inference="alignment:Splign:2.1.0"
     exon            2041..3327
                     /gene="Grk2"
                     /gene_synonym="Adrbk1; BARK1; GRK-2"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gcgggcgacg agcgaggacc gggcggggcg gcggcggcgc cgaggccgac aagccgcgcg
       61 ggccgggccg ggccgagcga gcgggagcgg agcggagcct cgggcggcgg gaggaacagc
      121 gccgccgccg ccaagatggc cgacctggag gcggtgctgg ccgatgtgag ctacctgatg
      181 gccatggaga agagcaaggc cacgccggcc gcgcgcgcca gcaagaagat cctgctgcca
      241 gagcccagca tccgcagcgt catgcagaag tatctagagg atcgaggaga agtgaccttt
      301 gaaaagatct tctcacagaa gttaggatac ctgcttttcc gggacttctg cctgaaccat
      361 ctggaagagg ccaagccctt ggtggagttc tatgaagaga tcaagaaata tgagaagctg
      421 gagacggagg aggaacgtgt ggtccgcagc cgggagatct ttgactccta catcatgaaa
      481 gaactgctgg cctgctcaca tcccttttca aagaatgcca cggagcatgt ccagggccac
      541 ctggtgaaga agcaggtacc tccagatctc ttccagccat acattgagga gatttgtcag
      601 aacctccgag gggatgtgtt ccacaagttc attgagagtg acaagttcac acggttctgc
      661 cagtggaaga atgtagagct caacatccac ctgaccatga atgacttcag tgtgcatcga
      721 atcatcgggc gtgggggctt cggtgaggtc tatgggtgcc ggaaagcaga cacaggcaag
      781 atgtacgcca tgaagtgtct ggacaagaaa cgcatcaaga tgaagcaggg agagaccctg
      841 gctctgaacg agcggatcat gctttccctc gtcagcactg gggactgccc cttcatcgtg
      901 tgcatgtcat atgcattcca cacaccagac aagctcagtt tcatcctgga tctcatgaac
      961 ggtggggacc tgcactacca cttgtctcag catggagtct tctctgaagc cgacatgcgc
     1021 ttctacgcag ctgagatcat cctgggcctt gaacacatgc acaatcgctt tgtagtctac
     1081 agggacctaa agccagccaa cattctcctg gatgagcatg gccatgtgag aatctcagac
     1141 ctgggcctag cctgtgactt ctccaagaag aagcctcatg ccagtgtggg cacacacggg
     1201 tacatggccc cggaagtcct acagaagggc gtggcctatg acagcagtgc cgactggttc
     1261 tccctgggct gcatgctctt caagttgttg cggggacaca gccccttccg gcagcacaag
     1321 accaaagaca agcatgagat tgaccgcatg acattgacaa tggctgttga gctgcctgac
     1381 tccttctccc ctgaactccg atccctgctg gaaggtttgc tgcagaggga tgtcaatcgg
     1441 agactaggct gtctgggccg tggggctcaa gaggtaaaag aaagcccctt cttccgttcc
     1501 ctggactggc agatggtctt cttacagaag taccctcctc cactgatccc cccacgcggg
     1561 gaggtgaatg cagcggacgc ctttgacatt ggctcctttg atgaggagga cacaaaagga
     1621 atcaagttac tggacagtga ccaggaactg taccgcaact tccctctcac catctctgag
     1681 cggtggcaac aggaggtggc agagactgtc tttgatacca tcaatgctga gacagaccgg
     1741 ctggaggcac ggaagaaagc caaaaacaaa cagctgggcc acgaggagga ctacgccctg
     1801 ggtaaggact gcatcatgca tggctacatg tccaagatgg gcaacccctt cctgacccag
     1861 tggcagcggc gatacttcta cctgttcccc aaccggcttg agtggagggg tgaaggcgag
     1921 gctccgcaga gcctcctgac catggaagag atccagtcgg tggaagagac acagatcaag
     1981 gaacgcaagt gtctcctgct taagatccga ggtggcaagc agtttgtcct gcagtgtgat
     2041 agtgaccctg agctggtgca atggaaaaaa gagctgcgcg atgcttaccg tgaggcccag
     2101 cagctggtgc agcgagtgcc caagatgaag aacaagccac gctcacctgt ggtggagctg
     2161 agcaaggtgc cactgattca gcgtggcagt gccaacggcc tctgattcac ccatctgctg
     2221 cccgcctgcc cacccgcctt ttataaacct ctaatttatt ttgttgaatt tttattattt
     2281 gttttcccgc aaagcggaaa aggttttatt ttgtaattat tgtgatttcc tgtggcccca
     2341 gcctggccca gcccccaggg aggggcctgc ttgcctcagc tcctgctgcc accaacccag
     2401 ccactgtcta gcacccctgc cccgccccca ccgcatcccc ctagggtcct tgctcccagt
     2461 atcttcctac tgggaagagg agcaaccctc agcagccctc ttgcccctcc atggggtgaa
     2521 gccacagtcg gtgggtgtgt cacttcaggc tctgtgggct acactcagcc tgaggcccag
     2581 ctgggtggcc cagcacccct accctaggaa ggtgcaacac agggatacta cttgaacttt
     2641 cctatcatag ctcccatgcc acagagggac agacctctct gtcctgccct cttgccactg
     2701 gtgagagaag gcccttgtct ccccagactt ggcggtctcc cacagtgacg ggctcctggg
     2761 tccttgttta ggaaaagcct gtgtgtgaca cctgcctcat caggaggctg ccattggcag
     2821 tggctgctgc tggcctccct gcaagtcccc tcttcccatt gcgtgcctgg actcaggagc
     2881 atagaaaaac gactgtagat tgttgggcca cactggccta gcctgaacct agatccctaa
     2941 ctctgggctg ggtgaggctc atctgcccag gaccacagtg ggacagcctg ggcaggtggg
     3001 tggcacagca taagaaccca gctggggccg gccctccctg tcctggctgg tcagtgtgcc
     3061 cccacccaca ctgtccagcc ccctagccca tgtcatgtca gtggccacca tccacaggtg
     3121 ccaccttgcc caccgcatgc ccccttgtgc cagtcgcgca gctgtgtgtg gtgtcgcgcc
     3181 ctctccctct ggggctgggt gggcgcaccc tcccctattg tctactcact cctggggcgt
     3241 ttctttgccg atttttgaat gtgattttaa agagtggaaa atgagactat gcgtttttat
     3301 aaaaaatggt gcctgactcc ttgctgt
//
