LOCUS       NM_012573              15639 bp    mRNA    linear   ROD 13-DEC-2024
DEFINITION  Rattus norvegicus glutamate ionotropic receptor NMDA type subunit
            2A (Grin2a), mRNA.
ACCESSION   NM_012573 XM_346384
VERSION     NM_012573.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 15639)
  AUTHORS   Schmidt,C.C., Tong,R. and Emptage,N.J.
  TITLE     GluN2A- and GluN2B-containing pre-synaptic N-methyl-d-aspartate
            receptors differentially regulate action potential-evoked Ca2+
            influx via modulation of SK channels
  JOURNAL   Philos Trans R Soc Lond B Biol Sci 379 (1906), 20230222 (2024)
   PUBMED   38853550
  REMARK    GeneRIF: GluN2A- and GluN2B-containing pre-synaptic
            N-methyl-d-aspartate receptors differentially regulate action
            potential-evoked Ca[2+] influx via modulation of SK channels.
REFERENCE   2  (bases 1 to 15639)
  AUTHORS   Deep,S.N., Seelig,S., Paul,S. and Poddar,R.
  TITLE     Homocysteine-induced sustained GluN2A NMDA receptor stimulation
            leads to mitochondrial ROS generation and neurotoxicity
  JOURNAL   J Biol Chem 300 (5), 107253 (2024)
   PUBMED   38569938
  REMARK    GeneRIF: Homocysteine-induced sustained GluN2A NMDA receptor
            stimulation leads to mitochondrial ROS generation and
            neurotoxicity.
REFERENCE   3  (bases 1 to 15639)
  AUTHORS   Huang,H., Jiang,H. and Sun,H.
  TITLE     GluN2A Mediates PS-Induced Depressive-Like Behavior by Activating
            CaMKII and Inhibiting Myelinization
  JOURNAL   Front Biosci (Landmark Ed) 28 (12), 347 (2023)
   PUBMED   38179739
  REMARK    GeneRIF: GluN2A Mediates PS-Induced Depressive-Like Behavior by
            Activating CaMKII and Inhibiting Myelinization.
REFERENCE   4  (bases 1 to 15639)
  AUTHORS   Zhang,L., Luo,L., Xue,L., Ran,D., Yang,F., Tang,Q. and Jiang,W.
  TITLE     RAGE signaling pathway is involved in CUS-induced depression-like
            behaviors by regulating the expression of NR2A and NR2B in rat
            hippocampus DG
  JOURNAL   Exp Neurol 361, 114299 (2023)
   PUBMED   36521778
  REMARK    GeneRIF: RAGE signaling pathway is involved in CUS-induced
            depression-like behaviors by regulating the expression of NR2A and
            NR2B in rat hippocampus DG.
REFERENCE   5  (bases 1 to 15639)
  AUTHORS   Salman,T., Afroz,R., Nawaz,S., Mahmood,K., Haleem,D.J. and
            Zarina,S.
  TITLE     Differential effects of memory enhancing and impairing doses of
            methylphenidate on serotonin metabolism and 5-HT1A, GABA, glutamate
            receptor expression in the rat prefrontal cortex
  JOURNAL   Biochimie 191, 51-61 (2021)
   PUBMED   34454977
  REMARK    GeneRIF: Differential effects of memory enhancing and impairing
            doses of methylphenidate on serotonin metabolism and 5-HT1A, GABA,
            glutamate receptor expression in the rat prefrontal cortex.
REFERENCE   6  (bases 1 to 15639)
  AUTHORS   Kornau,H.C., Schenker,L.T., Kennedy,M.B. and Seeburg,P.H.
  TITLE     Domain interaction between NMDA receptor subunits and the
            postsynaptic density protein PSD-95
  JOURNAL   Science 269 (5231), 1737-1740 (1995)
   PUBMED   7569905
REFERENCE   7  (bases 1 to 15639)
  AUTHORS   Sakimura,K., Kutsuwada,T., Ito,I., Manabe,T., Takayama,C.,
            Kushiya,E., Yagi,T., Aizawa,S., Inoue,Y., Sugiyama,H. et al.
  TITLE     Reduced hippocampal LTP and spatial learning in mice lacking NMDA
            receptor epsilon 1 subunit
  JOURNAL   Nature 373 (6510), 151-155 (1995)
   PUBMED   7816096
REFERENCE   8  (bases 1 to 15639)
  AUTHORS   Kutsuwada,T., Kashiwabuchi,N., Mori,H., Sakimura,K., Kushiya,E.,
            Araki,K., Meguro,H., Masaki,H., Kumanishi,T., Arakawa,M. et al.
  TITLE     Molecular diversity of the NMDA receptor channel
  JOURNAL   Nature 358 (6381), 36-41 (1992)
   PUBMED   1377365
REFERENCE   9  (bases 1 to 15639)
  AUTHORS   Monyer,H., Sprengel,R., Schoepfer,R., Herb,A., Higuchi,M.,
            Lomeli,H., Burnashev,N., Sakmann,B. and Seeburg,P.H.
  TITLE     Heteromeric NMDA receptors: molecular and functional distinction of
            subtypes
  JOURNAL   Science 256 (5060), 1217-1221 (1992)
   PUBMED   1350383
REFERENCE   10 (bases 1 to 15639)
  AUTHORS   Meguro,H., Mori,H., Araki,K., Kushiya,E., Kutsuwada,T.,
            Yamazaki,M., Kumanishi,T., Arakawa,M., Sakimura,K. and Mishina,M.
  TITLE     Functional characterization of a heteromeric NMDA receptor channel
            expressed from cloned cDNAs
  JOURNAL   Nature 357 (6373), 70-74 (1992)
   PUBMED   1374164
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000010.1 and M91561.1.
            
            On Mar 10, 2021 this sequence version replaced NM_012573.3.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: M91561.1, SRR26360176.2312356.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760393, SAMEA5760433
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1001              JAXUCZ010000010.1  6136458-6137458
            1002-3319           M91561.1           1-2318
            3320-3391           JAXUCZ010000010.1  6483121-6483192
            3392-3579           JAXUCZ010000010.1  6513221-6513408
            3580-3818           JAXUCZ010000010.1  6541798-6542036
            3819-15639          JAXUCZ010000010.1  6548183-6560003
FEATURES             Location/Qualifiers
     source          1..15639
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="10"
                     /map="10q11"
     gene            1..15639
                     /gene="Grin2a"
                     /gene_synonym="GluN2A; NMDAR2A; NR2A"
                     /note="glutamate ionotropic receptor NMDA type subunit 2A"
                     /db_xref="GeneID:24409"
                     /db_xref="RGD:2737"
     exon            1..1014
                     /gene="Grin2a"
                     /gene_synonym="GluN2A; NMDAR2A; NR2A"
                     /inference="alignment:Splign:2.1.0"
     exon            1015..1205
                     /gene="Grin2a"
                     /gene_synonym="GluN2A; NMDAR2A; NR2A"
                     /inference="alignment:Splign:2.1.0"
     exon            1206..1637
                     /gene="Grin2a"
                     /gene_synonym="GluN2A; NMDAR2A; NR2A"
                     /inference="alignment:Splign:2.1.0"
     CDS             1224..5618
                     /gene="Grin2a"
                     /gene_synonym="GluN2A; NMDAR2A; NR2A"
                     /note="glutamate [NMDA] receptor subunit epsilon-1;
                     glutamate receptor ionotropic, NMDA 2A;
                     N-methyl-D-aspartate receptor subunit 2A; N-methyl
                     D-aspartate receptor subtype 2A; glutamate receptor,
                     ionotropic, N-methyl D-aspartate 2A"
                     /codon_start=1
                     /product="glutamate receptor ionotropic, NMDA 2A
                     precursor"
                     /protein_id="NP_036705.3"
                     /db_xref="GeneID:24409"
                     /db_xref="RGD:2737"
                     /translation="MGRLGYWTLLVLPALLVWRDPAQNAAAEKGPPALNIAVLLGHSH
                     DVTERELRNLWGPEQATGLPLDVNVVALLMNRTDPKSLITHVCDLMSGARIHGLVFGD
                     DTDQEAVAQMLDFISSQTFIPILGIHGGASMIMADKDPTSTFFQFGASIQQQATVMLK
                     IMQDYDWHVFSLVTTIFPGYRDFISFIKTTVDNSFVGWDMQNVITLDTSFEDAKTQVQ
                     LKKIHSSVILLYCSKDEAVLILSEARSLGLTGYDFFWIVPSLVSGNTELIPKEFPSGL
                     ISVSYDDWDYSLEARVRDGLGILTTAASSMLEKFSYIPEAKASCYGQAEKPETPLHTL
                     HQFMVNVTWDGKDLSFTEEGYQVHPRLVVIVLNKDREWEKVGKWENQTLSLRHAVWPR
                     YKSFSDCEPDDNHLSIVTLEEAPFVIVEDIDPLTETCVRNTVPCRKFVKINNSTNEGM
                     NVKKCCKGFCIDILKKLSRTVKFTYDLYLVTNGKHGKKVNNVWNGMIGEVVYQRAVMA
                     VGSLTINEERSEVVDFSVPFVETGISVMVSRSNGTVSPSAFLEPFSASVWVMMFVMLL
                     IVSAIAVFVFEYFSPVGYNRNLAKGKAPHGPSFTIGKAIWLLWGLVFNNSVPVQNPKG
                     TTSKIMVSVWAFFAVIFLASYTANLAAFMIQEEFVDQVTGLSDKKFQRPHDYSPPFRF
                     GTVPNGSTERNIRNNYPYMHQYMTRFNQRGVEDALVSLKTGKLDAFIYDAAVLNYKAG
                     RDEGCKLVTIGSGYIFATTGYGIALQKGSPWKRQIDLALLQFVGDGEMEELETLWLTG
                     ICHNEKNEVMSSQLDIDNMAGVFYMLAAAMALSLITFIWEHLFYWKLRFCFTGVCSDR
                     PGLLFSISRGIYSCIHGVHIEEKKKSPDFNLTGSQSNMLKLLRSAKNISNMSNMNSSR
                     MDSPKRATDFIQRGSLIVDMVSDKGNLIYSDNRSFQGKDSIFGDNMNELQTFVANRHK
                     DNLSNYVFQGQHPLTLNESNPNTVEVAVSTESKGNSRPRQLWKKSMESLRQDSLNQNP
                     VSQRDEKTAENRTHSLKSPRYLPEEVAHSDISETSSRATCHREPDNNKNHKTKDNFKR
                     SMASKYPKDCSDVDRTYMKTKASSPRDKIYTIDGEKEPSFHLDPPQFVENITLPENVG
                     FPDTYQDHNENFRKGDSTLPMNRNPLHNEDGLPNNDQYKLYAKHFTLKDKGSPHSEGS
                     DRYRQNSTHCRSCLSNLPTYSGHFTMRSPFKCDACLRMGNLYDIDEDQMLQETGNPAT
                     REEVYQQDWSQNNALQFQKNKLRINRQHSYDNILDKPREIDLSRPSRSISLKDRERLL
                     EGNLYGSLFSVPSSKLLGNKSSLFPQGLEDSKRSKSLLPDHASDNPFLHTYGDDQRLV
                     IGRCPSDPYKHSLPSQAVNDSYLRSSLRSTASYCSRDSRGHSDVYISEHVMPYAANKN
                     TMYSTPRVLNSCSNRRVYKKMPSIESDV"
     exon            1638..2230
                     /gene="Grin2a"
                     /gene_synonym="GluN2A; NMDAR2A; NR2A"
                     /inference="alignment:Splign:2.1.0"
     exon            2231..2345
                     /gene="Grin2a"
                     /gene_synonym="GluN2A; NMDAR2A; NR2A"
                     /inference="alignment:Splign:2.1.0"
     exon            2346..2551
                     /gene="Grin2a"
                     /gene_synonym="GluN2A; NMDAR2A; NR2A"
                     /inference="alignment:Splign:2.1.0"
     exon            2552..2720
                     /gene="Grin2a"
                     /gene_synonym="GluN2A; NMDAR2A; NR2A"
                     /inference="alignment:Splign:2.1.0"
     exon            2721..2874
                     /gene="Grin2a"
                     /gene_synonym="GluN2A; NMDAR2A; NR2A"
                     /inference="alignment:Splign:2.1.0"
     exon            2875..3000
                     /gene="Grin2a"
                     /gene_synonym="GluN2A; NMDAR2A; NR2A"
                     /inference="alignment:Splign:2.1.0"
     exon            3001..3230
                     /gene="Grin2a"
                     /gene_synonym="GluN2A; NMDAR2A; NR2A"
                     /inference="alignment:Splign:2.1.0"
     exon            3231..3391
                     /gene="Grin2a"
                     /gene_synonym="GluN2A; NMDAR2A; NR2A"
                     /inference="alignment:Splign:2.1.0"
     exon            3392..3579
                     /gene="Grin2a"
                     /gene_synonym="GluN2A; NMDAR2A; NR2A"
                     /inference="alignment:Splign:2.1.0"
     exon            3580..3818
                     /gene="Grin2a"
                     /gene_synonym="GluN2A; NMDAR2A; NR2A"
                     /inference="alignment:Splign:2.1.0"
     exon            3819..15639
                     /gene="Grin2a"
                     /gene_synonym="GluN2A; NMDAR2A; NR2A"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 tgacatcaga gtgggttccc ataccaggat ggtgaggcat tgagaggagc atcctgggcg
       61 ggtgtgtgcg tgtcggcgtg ccctgcgtgg agcagggatt gctgcaccca gcgccaggca
      121 ccggaggagg aggaatccgt agcagagatc tgaataattg attcttcagc agcaccagac
      181 gccggggagc gggcggagag cgtggtttca gcatcaaggt tgcgcgccac cgcaggctcc
      241 ccccgtcacc agccagacac ctaggtcggc cttgttccgc tgccgccgct gccgccgtgc
      301 gctccagaag agggtggccg cgctccgcgt cgccgcctag ggactgcggg gtctgcctcg
      361 gccagcctcc ggggtgggcg actgcaggcg gaccagagct ccaaaaaaac cgtcggattg
      421 cgcgggggtt gctgcgttgc gtctcgctgg ctgagaacaa gtcctaagtt ggtgtggtgg
      481 gtgcatctgc tttccttatc ctcctaggcg accgcagcag aagcgcgccc caggatcctg
      541 gcttctcctg tgttgtgagc cgcggtggcc aggcactctc ctgggctccc gctgaccaag
      601 tgaggaaccg aacgcccagc gcggtgctgg ccccgcagcg aagggcccgg agggagctgc
      661 ggtcctccgc ccgcgccctt ctcggccagg gaccgcgggg atccccggcc acacgcgcgc
      721 gcgcactccc acactcactc actcacacac acacatacac acacacacac tcacacactc
      781 acgcacgcca agcaggagca ggactgcgga gagggtgtcc aggagcgctc agaggaccgg
      841 cagtcgctgt ccggagtgga acagaaagct gagccagggc tctagaagag aggctcctga
      901 ggtgctgtgc ctgagcatgg ggctggatga ggtctgagag tcgcggcggc agcaatcagc
      961 cctggagatg accaggggtg gccactgctg agaactatgt ggagagaggc tgcgagccct
     1021 gctgcagagc ctccggctgg gatagccgcc ccccgtgggg gtgatgcgga cagcgcggga
     1081 cagccagggg agcgcgcggg ggccgcagca tgcgggaacc cgctaaacct ggtggctgct
     1141 gaggcggccg agatgctcgt gcgcgcagca cgccccattg catcctccac cttctccggc
     1201 tacagggacc ctaagtggcg accatgggca gattgggcta ctggaccttg ctggtattgc
     1261 cggcccttct ggtctggcgc gatccggcgc agaacgcggc ggcggagaag ggtcctccag
     1321 cgctgaacat tgcggtgctg ctgggtcaca gccacgacgt gacagaacgc gaacttcgaa
     1381 atctgtgggg cccagagcag gcaaccggct tgcccctgga tgtgaacgtg gtggcgttat
     1441 tgatgaaccg cactgaccct aagagcctca tcacgcatgt gtgcgacctc atgtccgggg
     1501 cgcgcatcca cggcttggtg tttggagatg acacggacca ggaggctgtg gcccagatgc
     1561 tggattttat ctcctcacag acttttatcc ccatcttggg cattcatggg ggtgcatcta
     1621 tgatcatggc tgacaaggat ccgacatcca cgttcttcca gtttggagcc tccatccagc
     1681 agcaagccac agttatgctg aagatcatgc aggactacga ctggcacgtc ttctccctgg
     1741 tcaccaccat cttccctggc taccgagact tcatcagctt tatcaagaca acagtggaca
     1801 acagctttgt gggctgggat atgcagaacg tgatcacact ggacacctcc ttcgaggacg
     1861 ccaagacgca ggtccagctg aagaagatcc attcttctgt catcctgctc tactgctcca
     1921 aagacgaggc tgtcctcatc ctgagcgagg ctcgctccct cggcctcact ggctatgatt
     1981 tcttctggat tgtccccagt ttggtgtctg ggaacacaga gctcatcccc aaagagtttc
     2041 catcaggtct catttcagtc tcttatgacg actgggacta cagcctggag gcaagagtga
     2101 gagacggtct tgggatctta accactgccg catcctccat gttggagaag ttctcctaca
     2161 ttcctgaggc caaggccagc tgctatgggc aggcagagaa gccagagacc ccgctacaca
     2221 ccctgcacca attcatggtc aatgtgactt gggatggcaa ggacttgtcc ttcactgagg
     2281 aaggttacca ggtgcacccc aggcttgtgg tgatcgtgct gaacaaggac cgggagtggg
     2341 aaaaggtggg caagtgggag aaccagacgc tgagcctgag gcacgctgtg tggccaaggt
     2401 acaagtcctt ttctgactgc gagccagatg acaaccacct cagcattgtc accttggagg
     2461 aagccccctt cgtcatcgta gaggacatag accccctgac tgagacctgt gtgaggaaca
     2521 cggtgccctg tcggaagttc gtcaagatca acaattcaac caacgaaggg atgaatgtga
     2581 agaaatgctg caaggggttc tgcatcgaca tcctcaagaa gctgtccaga actgtgaagt
     2641 tcacctatga cctctacctg gtgaccaatg ggaagcatgg gaaaaaggtt aacaatgtgt
     2701 ggaatggaat gataggtgaa gtggtctatc aacgagcagt catggctgtg ggctcactca
     2761 ccatcaatga ggagcgttcg gaagtggtgg acttctcggt gcccttcgtg gagacaggaa
     2821 tcagcgtcat ggtctccagg agtaatggca ctgtctcccc ttctgctttc ctcgaaccct
     2881 tcagtgcctc cgtctgggtg atgatgtttg tgatgctgct catcgtctca gccattgctg
     2941 tcttcgtttt tgaatacttc agtcctgttg gatacaacag aaacttagcc aaagggaaag
     3001 ctccccacgg gccttctttt actattggaa aagctatatg gctcctctgg ggcctggtct
     3061 tcaacaattc tgtgcctgtc cagaatccta aaggcacaac cagcaagatc atggtgtcag
     3121 tgtgggcctt ctttgctgtc atcttcctgg ccagttacac agccaacctg gctgccttca
     3181 tgatccagga ggagtttgtg gaccaagtga ctggcctcag tgacaagaag ttccagagac
     3241 ctcatgacta ttctccacct ttccgatttg ggacggtacc caatggaagt acagagagga
     3301 atattcgtaa caactacccg tatatgcacc agtacatgac cagattcaac cagaggggag
     3361 tggaggatgc cttggtcagc ttgaaaaccg ggaagttgga cgctttcatc tatgacgcag
     3421 ccgtcttgaa ctacaaggcc gggagggatg aaggctgtaa actggtgacc attgggagcg
     3481 ggtacatctt tgctaccaca ggctatggaa ttgcgctgca gaagggctca ccctggaaga
     3541 ggcagattga cctcgctctg ctccagtttg ttggtgatgg tgagatggag gagctggaga
     3601 cactgtggct tacgggcatc tgccacaacg agaagaatga ggtgatgagt agccagctgg
     3661 acatcgataa catggcgggc gtgttctaca tgctggctgc agccatggcc ctcagcctca
     3721 tcaccttcat ctgggagcac ctcttctact ggaagctgcg cttctgcttc acaggcgtgt
     3781 gctctgaccg gcccgggctg ctcttctcca tcagcagggg catctatagt tgcatccatg
     3841 gggtacacat tgaagaaaag aagaaatctc cagatttcaa tctgactgga tcacagagca
     3901 acatgctaaa gcttcttcgg tcagctaaaa acatctccaa tatgtccaac atgaactcct
     3961 caagaatgga ctcacctaaa agagctactg acttcattca aagagggtca cttattgtgg
     4021 acatggtttc agacaaggga aatttgatat attcagacaa cagatccttt caagggaagg
     4081 acagtatatt tggagacaac atgaatgaac tccaaacatt tgtggccaac aggcacaagg
     4141 ataatctcag taactatgtg tttcaaggac agcatcctct cactctcaat gagtccaacc
     4201 ctaacacagt agaggtggct gtcagcactg aatccaaagg gaactcccga ccccggcagc
     4261 tttggaagaa atccatggag tctctacgcc aggattctct aaaccagaac ccagtctccc
     4321 agagggatga gaagactgca gagaatcgga cccactcgct aaagagtcct aggtatcttc
     4381 cagaagaggt agcccactct gacatttcag aaacctcaag ccgggccaca tgccacaggg
     4441 agccagataa caataagaac cacaagacca aggataactt caaacggtca atggcctcta
     4501 agtatcccaa ggactgtagc gatgttgacc gcacctacat gaaaaccaaa gcaagttctc
     4561 ccagggataa gatctatacc attgatggtg agaaggagcc cagcttccac ttagatcctc
     4621 ctcagtttgt tgagaatata accctgcctg agaatgtggg cttcccagat acctaccaag
     4681 atcacaatga gaacttccgc aagggggact ccacactgcc catgaacagg aacccattac
     4741 ataatgaaga cgggcttccc aacaatgacc aatataaact ctatgccaag cactttacct
     4801 tgaaagacaa gggttcccca cacagtgagg gcagtgatcg ataccggcag aactccacac
     4861 attgcagaag ctgcctttcg aatctgccca cctactcagg ccactttacc atgaggtctc
     4921 ctttcaagtg tgatgcctgt ctgcggatgg ggaatctcta tgacattgat gaagaccaga
     4981 tgcttcagga gacaggtaac ccagctactc gggaggaggt ctaccagcag gactggtcac
     5041 agaacaacgc cctccagttc cagaagaaca agctaaggat taaccgacag cactcctatg
     5101 ataacattct ggacaaaccc agagagatag accttagcag gccctcccgg agcataagcc
     5161 tcaaggacag ggaacggcta ctggagggca acttgtatgg gagcctgttc agtgtcccct
     5221 caagcaaact cttggggaac aaaagctccc ttttccccca aggtctggag gacagcaaga
     5281 ggagcaagtc tctcttgcca gaccacgcct ccgataatcc tttcctccac acgtatgggg
     5341 atgaccaacg cttagttatc gggagatgtc cctcggaccc ttacaaacac tcattgccat
     5401 cacaggcggt aaatgacagc tatcttcggt catccttgag gtcaacagca tcatattgct
     5461 ccagggacag tcggggccac agtgatgtgt atatttcaga gcatgttatg ccttatgctg
     5521 caaataagaa taccatgtac tctaccccca gggttttaaa ttcctgcagc aatagacgag
     5581 tgtacaagaa aatgcctagt atcgaatctg atgtttaaga tcttccatca gtatttatct
     5641 ataaggaaac atatagaatg gccaacatta tagagggtaa atgttggatg tccgatagca
     5701 ccctactagg aggaagaggg tacagggagg tactttttgt tggctctttt gcacatggct
     5761 ccatgccata atcttccact caaggaatct tgtgagatat gtgctgagca cagcatatac
     5821 cacgtaggtg aatccttaac caaaaacaaa taaatacaca tgggcaagtc tcccagacat
     5881 ggcgactggg tacggcggca ataatggtgc atcagacggc gatggtgaca ttgtggttgc
     5941 ctatattcca aattccatga agatcaatcc accatgtaac ttcccccatc agaaatgcct
     6001 cacagtttct ctaatacaga ataagcaata tggtatgcat gtaagtctga cgcagacaat
     6061 aagacagtta agaatgcatc tgcactgtag taagattgac atgtgcaagg attaagaagt
     6121 ttggcttgta acagttctca gatttcttgt tatgccttcc atgacaaccc tggatcagtc
     6181 ccccttaaac tccaggttcg ccccccccca aagaatagca aatcagcgtg tttgtggcac
     6241 ccacactact ttcattgtag gcttccccca agacacacct ggaacaaata gccccaggga
     6301 ctctcaggtg gagaactgta agaatctctt tggatgttaa tgtgtgtgta tctctcaccc
     6361 tcagcttcat tggtcttgtt cggagctgca taaacacttt tatgtcttcg atatctaagt
     6421 gtggatcttc tgtctatgac ataatggctg ttgtagttaa tcttgaagac tcatgtacgt
     6481 aagtctgcat ttcctccctt ctggtgatga ctcagggttg tatagtatct cttacctgac
     6541 ccctcccaga gtaacgtaac ttgttctgtc cccagacagc catgatggtg tctaactagc
     6601 tgtgtgttgt gttcctagag ttgttaatgt ggtgacatgt gccagcagcc atgtgtaatc
     6661 tgtaagaagt cccatgctat ctgtgtgtcc aaggctagca tggttttaca tttatgtccc
     6721 tcaaagtcag gaaactctta gacaattatt tctatacaaa cctcctcaaa tcaaaagcct
     6781 tcagacatca gccacttgtg tgtccccccc caagcaccct tccaactgtg gataaactca
     6841 gtaaaacaag ggatttctat cctctgaaat gtacaccttc ttggaggtga atttcttccc
     6901 cagcaccaac agaaaccaat gagaagtctt caacgcattt gtcatttctg agctgcctgc
     6961 attgtcctat tcttggccca ctgagcctgt gcatatatga cagaacagat actcattaga
     7021 ttaaaaaaaa gaaaaactgg agccaatgag gggaatattc gtgcatggaa aatgtcttag
     7081 cacataattt tttatcacat ttttaaaaag gactcaaaag gtagttatga taccttatta
     7141 gacaaggaca ttcacaaaag cagaaaataa tatgaattaa acattttata tgcttctttt
     7201 ctagcaatta gtatatttcc aatgcattat tgtccttgaa atagccaggc tgctgaccgt
     7261 ggtatttcgc aggagacttt agttggttgg gtaactctct cccatgaagg attctaacca
     7321 tttcttgttt ttcctcttac tgaatcttgg cttgggttct tggagaaagg gatcgcacga
     7381 caggttgaaa tttcatccct gttcttttct tcgtggtgtt ctattttata ggtgggattg
     7441 tccaactaca agttaagcag ggaaggttct cttagagctt ggggctgctt cagaaggagc
     7501 tagcagaggg aaagttcatt tgacctacag tgttgcagaa tgacaaaaag tgatgcaata
     7561 caccggagga ccagaggtgg agtggcagag ggggtgagga tctctcttcc tgtgctcttg
     7621 cagaacacag aaggaggctg gggtggagct agagggctga agtcacagga ctagggtgga
     7681 gccagcggtc tggggcggag ctagaaggct gaggtcacag gactagggtg gagccagtgg
     7741 tctggggcgg agctagaagg ctggggcgga gctagaagtt gataagcttt aaagtcacgt
     7801 tttattcctt ggacagagcc atcctttcct tccccaagtc ccacagctgg ggaaatgtgc
     7861 attagagaac ctagaacagt ctccctgatt tttgatctta aatttgagct tcagtgtgtg
     7921 tagtgtcctt tcaccccttt tactttattt ccccatagga ttccacacct gtattcctcc
     7981 tgctaggact gctgttgtgt cctcacagaa ctgactctta agcctccctg gaatacagaa
     8041 tcatgacttg tcacaaatct gaggagcatt ctgctttccc ggattgtcct caaagcccag
     8101 ggtgctgttg aatgtcccac attttagtga gagtacaaat ggcatttata ctgcctcctc
     8161 aaaaccctct ctggaaaatt caggatcatt tctgctatta actctgtaca aaagaagcta
     8221 aaatgaggct tttggaagaa tcagtcacat aatgggaaag aatacgtttg ttttaacacc
     8281 aagatattta caaaaataag tcatggtagt taggggctgt gtggcaaaca tttgcttttc
     8341 cctctcctct aatttgctgc ctcttgcaca tgataacttc aaagaagaaa acgaggaggt
     8401 cacgttccaa gtgagaggta atcatgtggg cttgtttgaa ggaaagccaa gtgagatcca
     8461 tccttttcaa aactaacccc gagggtggca aatggcacct acggccggtg atgagtactg
     8521 agcaagaggc cttggcctgg gtatcactta gagggttccc acactaaacc caacctctga
     8581 gtccttagac ttacagatgc taagaagcca gtgtctctta actcaaaggc ctgatctttc
     8641 cctggactaa gtcagggcag ccgccctggg cagttgtctt ggcaattctt ttgttttcct
     8701 cattttggaa aggaccagac tggtcctcag atgtcaatac ttgaaagcaa acagccatgc
     8761 atctgtcctt gcacctgaac cggtgcaaat gctgtgcaca agagaagctc ccagagcatg
     8821 accttcactc ggccaaacct cggtgctctg ggggaacagg tccaccaccc caatgctgca
     8881 ggtttagata cttctggttc cagtgctgtg gagttaggca agcccccatg ggtctgtctt
     8941 cctgtcagta agacctgcat gcgactcaag ggatgaggac ctcaccaggc atggagaggg
     9001 atggtgactg ctctgtcctc agcactagtg actatctctt accatgctct gggttttctc
     9061 ctccctgcct caaaggatac ttgagaagac ttaatattta gctgtctggt ggaagcattg
     9121 agatctatcc atttcagcaa aaactgtcaa atcaattcac acttattttc tgggcagaga
     9181 aaaaaaatta caccttgttt acaaatgaaa tagtttcatt taaatgggac tttagccaag
     9241 gcagttaatt agtcaaaaga tccagggtcg tcacgcagtc tcatggtgtt tattccgttt
     9301 ggtatgtcta ccgctgatat gtatttaata ttctgcatct tctcaggaaa tgacaaatgc
     9361 cctgaattct ttgcaaatag aattgctaat gaaaaagaaa aagaaagttg gtgttaatta
     9421 aatgatgatt aaaaatttat ctcccttaaa actaaaaagt gcggctttag taattgcttg
     9481 tcatataaat aaataccttg cactgaactc ccttgtggtt taagacctaa ataaataccg
     9541 attcaagaac ctgaaatctt tcagagaagt tgaaggtgcc cggtagcatg atgcacattc
     9601 ctgtactgtt tctgtgtgag gaactattgt atgtgaatcc ttcagcaaag gatagaagct
     9661 atttcaacat ggagctgttt atcactaacc ttggctatag tagacttacc cggacctcaa
     9721 gagataggat ttaaacagga ggacgccaaa tttatcagtt catatttaat agtaattcaa
     9781 gctctaaaga aaagagccct agcgctggag aggtggctca gtggttaaga gcacagactg
     9841 gggctggaga gatggctcag tggttaagag cactggctgc ttttccagag gtcctgagtt
     9901 caaatcccag caaccacatg gtggctccca accatctgta actttagttc taggggattt
     9961 gacacctcac acagatatac atgcaggcaa agcatcaatg cacacaaaaa taaataactc
    10021 tttttaaaaa aggaagaaag aaaaacagac agaaaagaga ccttccttcc aaaggccttg
    10081 tcctcttgca cgtcagtcaa aacttgtctt gtctgtgcct ccctcttcct ttggataaat
    10141 ggggtgaaaa gtattcattg agagttccgg tatctctact tgaaaagttt ttccaatgca
    10201 gagttgccct tttccggtca gccattttcc tacgtactgt gttcatctct aattgtctct
    10261 gctgaggtcg gcttgataca gatcagagat gactaaatgc acttctgtta atacagttgc
    10321 tcatgtacac atgtatatat tatacatgct tggtttctac aatccacact taagtgtatt
    10381 atccaaacaa acctgtacat attgtaaagt ttaacgttaa aaattaaatg ggggtgatgt
    10441 tgtgaacaca cgggaaagcc attctgatta ttttgatagg agggtattta tgtagagaag
    10501 gcattctatt tttttaaata tcagctctag gaagggtgac agggctcagg gggatttgag
    10561 cactgatgta ctttgtcccc ttaaggatca aaatcatgga gagaagagtg caatctatgc
    10621 tgcttacgtt cacttttgac tatgatgccg gtgtacatgt gtaaacccat caaaagacat
    10681 agactttcta gttcttttct cttttggctc attctgtgcc tagaagtgct atcagcatag
    10741 aagaagttca cagtacctgc ttggaagggc cttagattaa atggattttg tagatcgata
    10801 ctaagatttg gggaaagggg acaagagagg gtaacaaggg acagtagtct gtctaagatg
    10861 aagtcagaaa gcactggagc tggggccgga acccaagact cagttctgat tcctagctga
    10921 gtatgatttg ctaggctgac atacgtctgg gtttcttctg ggaagacacc cttcacatgt
    10981 tcctctggag taggagtcag agaaagtcac aggactggcc tggctctcct tgcttcctgc
    11041 tctttgcaga tccaggcatg actctttgaa ttttgttttt ctttaagctc ctttgcaatc
    11101 ctcactctct caggatcccg aggcacaggg gacgtttctt caggggtcct ctagataaga
    11161 tccacagggg acatcattta aagtttcaaa ccaaagatga aagaaggctg gcagccacca
    11221 gtgactacac ggtgctgttg accatgtgac accaatctgc ccgtagggac tggacacagt
    11281 aacactttcc ctaatgtccg atttggggct aagggcattg aaatggacct ggaagtagag
    11341 cctcttcctg taagcagtcc ctggtttgaa aatcgaattc catcatacaa aagataagca
    11401 acgactgtga aacgaaaaca gattaaatgt tgaaaattag aaacagatag taatcctatt
    11461 gtaaggagac caaaaaatta attgatactg taatcaaaca ctcaagtcct cccttgagtt
    11521 ttaaggaact ttggaaaaac acagttggtg tcgctggaaa tagccttgaa ttttttgagc
    11581 cttctgagat ggtgaggtgg agcacaggag cagctaaaca aagggggata acctcatccc
    11641 tccttatttt ttgcacgagt aatcttgggt ctggctccaa ccagccctgc atggcttacc
    11701 aaagcatgtc tcaaatgctt caaaagggtt gaattagtcc ttcgaggatg ggaaaataga
    11761 ctgtgatgta aaagaaaaag ggaaaggagg aggttgggtc atttatgact tgtaaaatct
    11821 tttaatagtt cagggtgtgg atgtgtcagg ggagagagta ggggacagct ttgtaaagtc
    11881 ccaagtgcct actgctatac gtggcaaaag gaagcgtcag gtttgctagt gaaaacagag
    11941 ggcaggaagg tacagaagag aagggaacct ggtgtgtgtc tgggaggacg gagcgatgta
    12001 gatggtgtgg aagatgctca cacgcggtgt gtgccagtct gcacatgagg ctgttgcagt
    12061 ttctgcttgt cacaagtgtg taccgtgatc agcgtctgaa cattgaaata cacaactctg
    12121 tctatgctga agagagctta gaatttaagt gtgattgccc ccgttttgaa gataaggaaa
    12181 ctgaggcgaa tactgctcta agcctcacca gcaacaaggc ctaactgata aatactcgtg
    12241 tcagatgtac cctagtaatg tctgctttcc tccagagagg tcagactgga agcagaaagg
    12301 gatttactag tttggggtcc ccaggatttt ccctaagtgt cctatgaaag tggcctggtt
    12361 tggggtgtat caagaattcc tggggacccc atgtctgttc acagtccctt cccagactag
    12421 aacactgctc acgtctctcc cttccctgtg cttgcactca gcattaactt ttcctttatt
    12481 tcctgttttt tctctttttc tttgaaaact taccttctct aattagcatg tgcctaatct
    12541 taagtatctc tttcccaatc tcctttcttc tttaacagtt ggcatcaggg ctagtaccat
    12601 taatgtatgt atatagaatg cgttcatagg tgtgtggacc cagtcacaca catgcatatg
    12661 cacacactgc cttgtggtcc tcaatcaaaa ctgtcgtaaa caccatgacc cctttctaaa
    12721 cgggatgcta tttgcaaatt cactgatcct gccgttcatc tgcattggct tgtttctgcc
    12781 aggcagacag aatgaggttc agagataggg cagggaatgg ggaatagttt tgcataaatg
    12841 attgagtgct ttagtgtcag ggtgattgag ggccagctat taaaatgctg ttgttgtcgt
    12901 tgttgttgtt tcatttgtta aattgcctta gagatgcact cctggtcatt agtatcagga
    12961 taattgttga tgcaccaaca tttgttaact ttatcccaag gaagagtgtt gaactgggat
    13021 gcttccacag cactttatag aattggaggg tggcacggtg tcgaacacgc cacacagacg
    13081 atgctccaga gcacacgagt gtcatctgtg catcccctcc ctcccatgtt ctaaaccaca
    13141 cttcttttct ggcatttatg tttacagtga ggtggaacac actaagccca ttttatagat
    13201 gagaagactg agctcagatc acaggaaggt tgtttgggga ggctctggaa ttacattttc
    13261 atagaaaact cagtcatgat tctaaatgct tctgaggagt cttcaaacag tataagcacc
    13321 tttagcttga tttctgagaa taattctctg ggtattgtca ccctttgagt tgatagtcaa
    13381 aggcctctta acgtagaggt tactagggct agggatacag ctcaactagt tgtgtgtttg
    13441 cacagcacat atgatctggg ttgcctcccg agtgctgcaa gcagaagcca ggtgtggtgg
    13501 cacatgcctg tgattccggc acccaggagg cagaggcagg aggatcatga gttcaaggtt
    13561 atacccagac tacattgtga gttggaggcc aacttgaact acaagagact cagtcttaaa
    13621 aactaaaacc aaaccacaca ggtcattatt atttaatagc tactctgaga ccatgtgttt
    13681 ctgtaagaga tgaggagact gtcccaggga tgctgaacta tgcagaaagt ggagtgcctc
    13741 aatcaacagg gccaagagct tcacgtcttt cgagctgtat ttcaaagcac acaattctcc
    13801 tgtgaaccag aaccaaacta tttaaaacgg actccttccc cagattagct tcacaagcaa
    13861 ggcattcgca gagttcagga agaagcaaga cagaagctat caccactcac caattcctct
    13921 tagatctcat atcagcagaa tgatgactct gcattttcac agtgctgcat cattgctcat
    13981 ctcccgtagc cctgggtcat ggcttcttct gcttaattct cttccacacc tttgggaagg
    14041 ttttaaagtg aaccctccga aggatattag cataacacct agccaatacc acagatgcct
    14101 tttaaattaa taaggttaat tgttctccag ctgacagaag tttgtctttg gcattttcaa
    14161 tatttctcat tgatctgaca tttttctgtt tcaagctttt aaagggctca aatcaaaggc
    14221 tattgataac tgagcgaagc taaggttcag aaatgtacaa atacattgtt tggttgtttt
    14281 ttcatgagaa acaattacag actttgaatt cattgaaggt tttttacatt agccatgtgt
    14341 gatatattga attgtttctc ttcccagtca ggctaatttg aacgaacaaa cagtattgac
    14401 ttttaaaacc tattttcctt tttaaagtat cttaaatgta ttaagtctgt agacagagct
    14461 ctggggatgc agccattagg gaagtgctcc acgtcggaac agaagaatca gacagggttt
    14521 tgtggctcat ctgagcaatt gtctttgaaa ttgtgatgga tttggaaagc tcaaactgac
    14581 aactctaaca ttcacattag cctgactttt tccattggtt aggatgtaaa tgaacaaaaa
    14641 ttttctaaag actttaagtc agggatatgt aggaaacatg gttgttgtaa aacacaaata
    14701 ttcacaaaaa taaatgtggc tcagatgctt tccaagcctg agggtcccca aagatgtttt
    14761 tatggttgtc ttcaaagtgc gactgttaat cttgttcatt ccccccataa gacataggta
    14821 agagcatagc cttgccaaat taattggtag aactcatcat agtttatatg gcactggaat
    14881 gtctgtgtat gtgtacttat atattttgag ctgaatatca gacagaatta ggtgagttaa
    14941 ttggtggctt gaataagaaa taggtattaa agtcataaat agtgttctcc tcagacagta
    15001 agagaaggta aactattcat tttctagtcg tgctgtaaga cttgcataat gtacaagcta
    15061 cccattgccc acaggagagt tatcgaatta gacagcttat ccataaagcc tttccatatg
    15121 agtccatctt taacccagta tgaatgagat tgaactcatg cagaaggttc ccatccacag
    15181 ctacattaaa cagcgtctaa aactcacatc aggactggct tgatgtcgtg tacggtggat
    15241 ccaaggatag ctgtgtgctc tgtcagtggt ccgcatctgg gagtagatat tcacctccac
    15301 atcacagcag tttatactgt catgtctgat cagctgcatg aggaagtgct gatgaccgta
    15361 ttaactattt ccaagcatag ctacaaaact acacgtttgc ttaacacctt tcgatgatag
    15421 aggaagtata tactatggtt gtcttcttgt agtatgtctt ttttaatttt gttcaatgtg
    15481 atatcaactg ttttaaggaa tgggatttga catgtgtgtg caaattgata tgtacataat
    15541 tcccgaggca atgcaagtgc ccaccataca gtgtaaaaat gtaaatagag gaaatttcat
    15601 ttttctaata aagataattt ctgccaattg atatgaaaa
//
