LOCUS       NM_032990               5052 bp    mRNA    linear   ROD 26-APR-2024
DEFINITION  Rattus norvegicus glutamate ionotropic receptor AMPA type subunit 3
            (Gria3), transcript variant 1, mRNA.
ACCESSION   NM_032990
VERSION     NM_032990.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 5052)
  AUTHORS   Zhao,Y., Chen,S., Swensen,A.C., Qian,W.J. and Gouaux,E.
  TITLE     Architecture and subunit arrangement of native AMPA receptors
            elucidated by cryo-EM
  JOURNAL   Science 364 (6438), 355-362 (2019)
   PUBMED   30975770
REFERENCE   2  (bases 1 to 5052)
  AUTHORS   Koszegi,Z., Fiuza,M. and Hanley,J.G.
  TITLE     Endocytosis and lysosomal degradation of GluA2/3 AMPARs in response
            to oxygen/glucose deprivation in hippocampal but not cortical
            neurons
  JOURNAL   Sci Rep 7 (1), 12318 (2017)
   PUBMED   28951554
  REMARK    GeneRIF: Study shows that oxygen and glucose deprivation (OGD)
            causes endocytosis, lysosomal targeting and consequent degradation
            of GluA2- and GluA3-containing AMPARs, and that PICK1 is required
            for both OGD-induced GluA2 endocytosis and lysosomal sorting.
            GluA1-containing AMPARs resist OGD-induced endocytosis and PICK1
            binding to the endocytic adaptor AP2 is enhanced by OGD in
            hippocampal, but not cortical neurons.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 5052)
  AUTHORS   Gutierrez-Castellanos,N., Da Silva-Matos,C.M., Zhou,K., Canto,C.B.,
            Renner,M.C., Koene,L.M.C., Ozyildirim,O., Sprengel,R., Kessels,H.W.
            and De Zeeuw,C.I.
  TITLE     Motor Learning Requires Purkinje Cell Synaptic Potentiation through
            Activation of AMPA-Receptor Subunit GluA3
  JOURNAL   Neuron 93 (2), 409-424 (2017)
   PUBMED   28103481
REFERENCE   4  (bases 1 to 5052)
  AUTHORS   Levitz,J., Habrian,C., Bharill,S., Fu,Z., Vafabakhsh,R. and
            Isacoff,E.Y.
  TITLE     Mechanism of Assembly and Cooperativity of Homomeric and
            Heteromeric Metabotropic Glutamate Receptors
  JOURNAL   Neuron 92 (1), 143-159 (2016)
   PUBMED   27641494
  REMARK    GeneRIF: results lead to a model of cooperative activation of
            mGluRs that provides a framework for understanding how class C
            GPCRs couple extracellular binding to dimer reorganization and G
            protein activation.
REFERENCE   5  (bases 1 to 5052)
  AUTHORS   Aruscavage,P.J. and Bass,B.L.
  TITLE     A phylogenetic analysis reveals an unusual sequence conservation
            within introns involved in RNA editing
  JOURNAL   RNA 6 (2), 257-269 (2000)
   PUBMED   10688364
REFERENCE   6  (bases 1 to 5052)
  AUTHORS   Lomeli,H., Mosbacher,J., Melcher,T., Hoger,T., Geiger,J.R.,
            Kuner,T., Monyer,H., Higuchi,M., Bach,A. and Seeburg,P.H.
  TITLE     Control of kinetic properties of AMPA receptor channels by nuclear
            RNA editing
  JOURNAL   Science 266 (5191), 1709-1713 (1994)
   PUBMED   7992055
REFERENCE   7  (bases 1 to 5052)
  AUTHORS   Hollmann,M., Hartley,M. and Heinemann,S.
  TITLE     Ca2+ permeability of KA-AMPA--gated glutamate receptor channels
            depends on subunit composition
  JOURNAL   Science 252 (5007), 851-853 (1991)
   PUBMED   1709304
REFERENCE   8  (bases 1 to 5052)
  AUTHORS   Nakanishi,N., Shneider,N.A. and Axel,R.
  TITLE     A family of glutamate receptor genes: evidence for the formation of
            heteromultimeric receptors with distinct channel properties
  JOURNAL   Neuron 5 (5), 569-581 (1990)
   PUBMED   1699567
REFERENCE   9  (bases 1 to 5052)
  AUTHORS   Sommer,B., Keinanen,K., Verdoorn,T.A., Wisden,W., Burnashev,N.,
            Herb,A., Kohler,M., Takagi,T., Sakmann,B. and Seeburg,P.H.
  TITLE     Flip and flop: a cell-specific functional switch in
            glutamate-operated channels of the CNS
  JOURNAL   Science 249 (4976), 1580-1585 (1990)
   PUBMED   1699275
REFERENCE   10 (bases 1 to 5052)
  AUTHORS   Keinanen,K., Wisden,W., Sommer,B., Werner,P., Herb,A.,
            Verdoorn,T.A., Sakmann,B. and Seeburg,P.H.
  TITLE     A family of AMPA-selective glutamate receptors
  JOURNAL   Science 249 (4968), 556-560 (1990)
   PUBMED   2166337
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from X54656.1, JAXUCZ010000021.1,
            BC091324.1, EV762496.1, CB713194.1, BF559208.1, DV723256.1 and
            BQ205510.1.
            
            On Dec 20, 2007 this sequence version replaced NM_032990.1.
            
            Summary: Glutamate receptors are the predominant excitatory
            neurotransmitter receptors in the mammalian brain and are activated
            in a variety of normal neurophysiologic processes. These receptors
            are heteromeric protein complexes composed of multiple subunits,
            arranged to form ligand-gated ion channels. The classification of
            glutamate receptors is based on their activation by different
            pharmacologic agonists. The subunit encoded by this gene belongs to
            a family of AMPA (alpha-amino-3-hydroxy-5-methyl-4-isoxazole
            propionate)-sensitive glutamate receptors, and is subject to RNA
            editing (AGA->GGA; R->G). Alternative splicing at this locus
            results in different isoforms, which may vary in their signal
            transduction properties. [provided by RefSeq, Jul 2008].
            
            Transcript Variant: This variant (1) encodes isoform 1 (also known
            as flip isoform). RNA editing (AGA->GGA) changes Arg769Gly.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data because no single transcript was available
            for the full length of the gene. The extent of this transcript is
            supported by transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: X54656.1, SRR26360176.51918.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760389, SAMEA5760393
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            undergoes RNA editing  :: PMID: 10688364, 7992055
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-497               X54656.1           1-497
            498-499             JAXUCZ010000021.1  125178619-125178620
            500-3030            X54656.1           500-3030
            3031-3047           JAXUCZ010000021.1  125367681-125367697
            3048-3510           BC091324.1         1-463
            3511-4076           EV762496.1         155-720
            4077-4445           CB713194.1         127-495
            4446-4725           BF559208.1         194-473
            4726-5031           DV723256.1         204-509
            5032-5052           BQ205510.1         1-21                c
FEATURES             Location/Qualifiers
     source          1..5052
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="Sprague-Dawley"
                     /db_xref="taxon:10116"
                     /chromosome="X"
                     /map="Xq35"
     gene            1..5052
                     /gene="Gria3"
                     /gene_synonym="GluA3; GluR-3; GluR-C; GluR-K3; GLUR3"
                     /note="glutamate ionotropic receptor AMPA type subunit 3"
                     /db_xref="GeneID:29628"
                     /db_xref="RGD:70958"
     exon            1..147
                     /gene="Gria3"
                     /gene_synonym="GluA3; GluR-3; GluR-C; GluR-K3; GLUR3"
                     /inference="alignment:Splign:2.1.0"
     CDS             57..2723
                     /gene="Gria3"
                     /gene_synonym="GluA3; GluR-3; GluR-C; GluR-K3; GLUR3"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1; glutamate receptor, ionotropic, AMPA3 (alpha
                     3); AMPA-selective glutamate receptor 3; glutamate
                     receptor, ionotrophic, AMPA 3; glutamate receptor,
                     ionotropic, AMPA 3"
                     /codon_start=1
                     /product="glutamate receptor 3 isoform 1 precursor"
                     /protein_id="NP_116785.2"
                     /db_xref="GeneID:29628"
                     /db_xref="RGD:70958"
                     /translation="MGQSVLRAVFFLVLGLLGHSHGGFPNTISIGGLFMRNTVQEHSA
                     FRFAVQLYNTNQNTTEKPFHLNYHVDHLDSSNSFSVTNAFCSQFSRGVYAIFGFYDQM
                     SMNTLTSFCGALHTSFVTPSFPTDADVQFVIQMRPALKGAILSLLGYYKWEKFVYLYD
                     TERGFSVLQAIMEAAVQNNWQVTARSVGNIKDVQEFRRIIEEMDRRQEKRYLIDCEVE
                     RINTILEQVVILGKHSRGYHYMLANLGFTDILLERVMHGGANITGFQIVNNENPMVQQ
                     FIQRWVRLDEREFPEAKNAPLKYTSALTHDAILVIAEAFRYLRRQRVDVSRRGSAGDC
                     LANPAVPWSQGIDIERALKMVQVQGMTGNIQFDTYGRRTNYTIDVYEMKVSGSRKAGY
                     WNEYERFVPFSDQQISNDSSSSENRTIVVTTILESPYVMYKKNHEQLEGNERYEGYCV
                     DLAYEIAKHVRIKYKLSIVGDGKYGARDPETKIWNGMVGELVYGRADIAVAPLTITLV
                     REEVIDFSKPFMSLGISIMIKKPQKSKPGVFSFLDPLAYEIWMCIVFAYIGVSVVLFL
                     VSRFSPYEWHLEDNNEEPRDPQSPPDPPNEFGIFNSLWFSLGAFMQQGCDISPRSLSG
                     RIVGGVWWFFTLIIISSYTANLAAFLTVERMVSPIESAEDLAKQTEIAYGTLDSGSTK
                     EFFRRSKIAVYEKMWSYMKSAEPSVFTKTTADGVARVRKSKGKFAFLLESTMNEYIEQ
                     RKPCDTMKVGGNLDSKGYGVATPKGSALGTPVNLAVLKLSEQGILDKLKNKWWYDKGE
                     CGAKDSGSKDKTSALSLSNVAGVFYILVGGLGLAMMVALIEFCYKSRAESKRMKLTKN
                     TQNFKPAPATNTQNYATYREGYNVYGTESVKI"
     exon            148..306
                     /gene="Gria3"
                     /gene_synonym="GluA3; GluR-3; GluR-C; GluR-K3; GLUR3"
                     /inference="alignment:Splign:2.1.0"
     exon            307..546
                     /gene="Gria3"
                     /gene_synonym="GluA3; GluR-3; GluR-C; GluR-K3; GLUR3"
                     /inference="alignment:Splign:2.1.0"
     exon            547..734
                     /gene="Gria3"
                     /gene_synonym="GluA3; GluR-3; GluR-C; GluR-K3; GLUR3"
                     /inference="alignment:Splign:2.1.0"
     exon            735..788
                     /gene="Gria3"
                     /gene_synonym="GluA3; GluR-3; GluR-C; GluR-K3; GLUR3"
                     /inference="alignment:Splign:2.1.0"
     exon            789..950
                     /gene="Gria3"
                     /gene_synonym="GluA3; GluR-3; GluR-C; GluR-K3; GLUR3"
                     /inference="alignment:Splign:2.1.0"
     exon            951..1118
                     /gene="Gria3"
                     /gene_synonym="GluA3; GluR-3; GluR-C; GluR-K3; GLUR3"
                     /inference="alignment:Splign:2.1.0"
     exon            1119..1223
                     /gene="Gria3"
                     /gene_synonym="GluA3; GluR-3; GluR-C; GluR-K3; GLUR3"
                     /inference="alignment:Splign:2.1.0"
     exon            1224..1331
                     /gene="Gria3"
                     /gene_synonym="GluA3; GluR-3; GluR-C; GluR-K3; GLUR3"
                     /inference="alignment:Splign:2.1.0"
     exon            1332..1538
                     /gene="Gria3"
                     /gene_synonym="GluA3; GluR-3; GluR-C; GluR-K3; GLUR3"
                     /inference="alignment:Splign:2.1.0"
     exon            1539..1915
                     /gene="Gria3"
                     /gene_synonym="GluA3; GluR-3; GluR-C; GluR-K3; GLUR3"
                     /inference="alignment:Splign:2.1.0"
     exon            1916..2114
                     /gene="Gria3"
                     /gene_synonym="GluA3; GluR-3; GluR-C; GluR-K3; GLUR3"
                     /inference="alignment:Splign:2.1.0"
     exon            2115..2362
                     /gene="Gria3"
                     /gene_synonym="GluA3; GluR-3; GluR-C; GluR-K3; GLUR3"
                     /inference="alignment:Splign:2.1.0"
     exon            2363..2477
                     /gene="Gria3"
                     /gene_synonym="GluA3; GluR-3; GluR-C; GluR-K3; GLUR3"
                     /inference="alignment:Splign:2.1.0"
     exon            2478..2725
                     /gene="Gria3"
                     /gene_synonym="GluA3; GluR-3; GluR-C; GluR-K3; GLUR3"
                     /inference="alignment:Splign:2.1.0"
     exon            2726..5040
                     /gene="Gria3"
                     /gene_synonym="GluA3; GluR-3; GluR-C; GluR-K3; GLUR3"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 tgcgcccatg ctcttgtcag cttcgtttta ggcgtagcac agccaggcag aagaaaatgg
       61 ggcaaagcgt gctccgggcg gtcttctttt tagtcctggg gcttttgggt cattctcacg
      121 gaggattccc caacaccatc agcataggtg gacttttcat gagaaacacg gttcaggagc
      181 acagcgcttt ccgctttgct gtgcagttat acaacaccaa ccagaacacc actgagaagc
      241 ccttccattt gaattaccac gtagaccact tggattcctc caatagtttt tctgtgacta
      301 atgctttctg ctcccagttc tccagagggg tgtatgctat ctttggattc tatgaccaga
      361 tgtcaatgaa caccctgacc tccttctgtg gggccctgca cacatctttt gtcacaccta
      421 gctttcccac tgatgcagat gtgcagtttg tcatccagat gcgcccagcc ttgaagggtg
      481 ccattctgag tcttctcggt tactacaagt gggagaagtt tgtgtacctc tatgacacag
      541 aacgagggtt ttctgtccta caagcaatta tggaggcagc agtgcaaaac aactggcaag
      601 tgacagcaag gtctgtggga aacataaagg acgtccagga attcagacgc atcattgaag
      661 aaatggacag aaggcaggaa aaacgatact tgattgactg tgaagtcgaa aggattaaca
      721 caattttgga acaggttgtg atcctgggga agcattcaag aggctatcac tacatgcttg
      781 ctaacctggg ttttactgac attttactgg aaagagtcat gcatggggga gccaacatta
      841 caggtttcca gattgtcaac aatgaaaacc caatggttca gcagttcata cagcgctggg
      901 tgagactgga tgaaagggaa ttccctgaag ccaagaatgc accactgaag tatacatctg
      961 cgctgacaca tgacgcaata ttggtcatag cagaagcctt ccgatacctg aggagacaga
     1021 gagtggatgt ctcccgcaga ggcagtgctg gagactgctt agcaaatcct gctgtgccct
     1081 ggagtcaagg aattgatatt gagagagctc tgaaaatggt gcaagtacaa ggaatgactg
     1141 gaaacatcca atttgacact tatggacgta ggacaaatta taccattgat gtctatgaaa
     1201 tgaaagtctc gggttctcga aaagctggtt actggaacga atatgaaagg tttgtgccct
     1261 tctcagatca acaaatcagc aatgacagct catcctcaga gaaccggacc attgtagtga
     1321 ctaccattct ggaatcacca tatgtgatgt ataaaaagaa tcatgagcag ctggaaggaa
     1381 atgagcgcta tgaaggctac tgtgttgatt tagcctatga aatagccaaa cacgtaagga
     1441 tcaaatacaa attgtccatt gtcggtgatg ggaaatatgg cgccagagat ccagagacta
     1501 aaatatggaa tggcatggtt ggggaacttg tctatggaag agctgatata gctgttgctc
     1561 cactcactat aacattggtc cgtgaagaag tcatagattt ctcaaagcca tttatgagcc
     1621 tgggaatctc catcatgata aagaagcctc agaaatcaaa gccaggcgtc ttttcattcc
     1681 tggatccttt ggcttatgaa atctggatgt gcattgtctt cgcttacatt ggagtcagtg
     1741 tagttctctt cctagtcagc agatttagcc cttatgaatg gcacttggaa gacaacaatg
     1801 aagaacctcg tgacccacaa agccctcctg atcctcccaa tgaatttgga atatttaaca
     1861 gtctttggtt ttccttgggt gctttcatgc agcaaggatg tgatatttct ccaagatcac
     1921 tttctgggcg cattgttgga ggggtttggt ggttcttcac cctgatcata atctcttcct
     1981 acactgcaaa ccttgctgct ttcctgactg tggagaggat ggtgtcccct atagagagcg
     2041 ctgaagactt agccaagcag actgaaattg catatgggac cctggactct ggttcaacaa
     2101 aagaattttt cagacgatcc aaaattgctg tgtatgagaa aatgtggtct tacatgaaat
     2161 ccgcagagcc atctgtgttt accaaaacaa cagctgacgg ggtagcccga gttcggaagt
     2221 ccaagggaaa gttcgccttc ctgctggagt cgaccatgaa cgagtacatt gagcagagaa
     2281 agccgtgcga tacgatgaaa gttggtggaa atctggattc caaaggctat ggtgtggcaa
     2341 cccctaaagg ctcagcatta ggaacgcctg taaaccttgc agtattgaaa ctcagtgaac
     2401 aaggcatctt agacaagctg aaaaacaaat ggtggtacga taagggggaa tgtggagcca
     2461 aggactccgg gagtaaggac aagaccagtg ctctaagcct gagcaatgtg gcaggcgtgt
     2521 tctatatact tgtcggaggt ctgggcctgg ccatgatggt ggctttgata gaattctgtt
     2581 acaaatcacg ggcagagtcc aaacgcatga aactcacaaa gaacacccaa aactttaagc
     2641 ctgctcctgc caccaacact cagaattacg ctacatacag agaaggctac aacgtgtatg
     2701 gaacagaaag tgttaagatc tagggatccc ttcccaccag aagcatgcaa tgagaggaaa
     2761 tcactgaaaa cgtggctgct tcaaggatcc tgagccggat ttcactctcc ctggtgtcgg
     2821 gcatgacacg aatattgctg atggtgcaat gacctttcaa taggaaaaac tgattttttt
     2881 tttccttcag tgccttatgg aacactctga gacttgcgac aatgcaaacc atcattgaaa
     2941 tctttttgct ttgcttgaaa aaaaattaaa ataaaaccaa caaaaaatgg acatgcaaga
     3001 ttccagtatg cgaaaaaaat cttattaaaa acatcaattc aacaaaagcc attctttgat
     3061 accactgcac agtacacaaa accatgtttg ttaacacaca cacatgcaca cactctctca
     3121 cacacacacg cacacactct cacacacaca cactcacaca tacacacaca cacacacgca
     3181 gacatgcacg tacgtataca cactcacaca tacaaattta ttcgaattca gcaaatagac
     3241 ccatcttagc taaaagaaga attcctcctg atttaaaaga aaaaatctgt ctcccagtgt
     3301 tcgggcagac agactggcat ttcttctagg atctgcagat cagatgtttt tggtatttcc
     3361 tgttggtggt gatgttctgt gcacctattt ccttttgatg ttgctgaatt gtgtaaatct
     3421 ttagaatgta aatgtgacac ttaagaaaat tcaaatgctt tgggaaagag actgaatggt
     3481 gacttagtat tcttgcaatg gttttatggg aaaaaaatgt ttggagaact ccccatagtt
     3541 gaggtgaagg gattaataga gcttttgaaa ttggcttctg tgtgtaccca ggggcagggc
     3601 actatcaggc atacctctcg gtgctttcct aaaacggatc ccggagcttt cctagaagcc
     3661 tggaattaga gctcacagat ctgtttttct tgcttcagtg tgcattttaa gtcaatatag
     3721 ccgagtagtc gaatagtaag cattgaggtg agggaaatgt tgcctattct cccagatgtg
     3781 tttggaatat ctttctcaga aaacaacact gtgtttagct ctgctttctc tgctaagtat
     3841 gccttttaag tgtacaacac ggagacagga ccgtgttgca aggcaggcca gtggattcag
     3901 accacagttc tcaatctgac tttactcttg ctaggtctgt tttaccagct gttgcctgtt
     3961 accgcctgcc tgtggctctc cgtcagactg catgtgtcct tttctagttt gcaaagacta
     4021 agatgctttc ctaaacctat cgctaacctg agggacctaa gtaagcatca ctcccaggtc
     4081 cttgcttgga gcagtttctc tactgactca ccgaagatcg ccccactgct ccatgggctc
     4141 tcaagtaact aattgcaaac ctgctgttgg catcaccaga ccagtcagag gaaatagtct
     4201 ccgctcacta attacctcct atataacgac gtatgcttta tgtagttcag tagtatgctc
     4261 tcatcagtaa cgtgcattgg aaagtttcca gactgtacaa attaggagcc cacctccatt
     4321 tcccaagtgt gacccttaga tgcttagttg actcgctgca catttgctct cgtctttaga
     4381 aaagaaagga agaggtctcg ttccagtgaa acatttccag aaaggtgtaa tataaacttt
     4441 cattccagaa agtgtgtcat gagcaaataa ctcacctgtc agatttttaa atggtattga
     4501 acacaaaaga aagctgttgt gttttgtttt gttttgttct catgaaactg tgatctttca
     4561 acttatgaat gctgtaattg tcccagtgcg ggaagctaac gctgtgtgaa tatgaagttg
     4621 tataaagcaa accaaccgac ctatacacaa acacttttca taggcactgt ataaagagaa
     4681 atgtatgttt attgactcaa atcagttttt cagagaggaa actttactga gattaagaag
     4741 cgggtaaatt ggtttgttat ttaaaaaaaa aacttgcatg tttaaaaaaa tgttgattgc
     4801 ttcaaatttc tgctattaac ttcaagctat gggagtttgg cggtagtcaa ttgaggattt
     4861 ttttccaatt cttttctttt tgttgttaaa gctgtacttc aatgaacaga aaaattgcca
     4921 agcaaactaa tggccgtaaa agtgtaaatt gcatgtgtgg gcataattac cgaacctcat
     4981 tgccatgagg tattgtacaa agttttaata cattttgtaa ataaaattgt aaagaaagaa
     5041 aaaaaaaaaa aa
//
