LOCUS       NM_030846               2608 bp    mRNA    linear   ROD 07-OCT-2024
DEFINITION  Rattus norvegicus growth factor receptor bound protein 2 (Grb2),
            mRNA.
ACCESSION   NM_030846 XM_003750955 XM_003750956 XM_006247767
VERSION     NM_030846.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 2608)
  AUTHORS   Yu,X., Zeng,Y., Bao,M., Wen,J., Zhu,G., Cao,C., He,X. and Li,L.
  TITLE     Low-magnitude vibration induces osteogenic differentiation of bone
            marrow mesenchymal stem cells via miR-378a-3p/Grb2 pathway to
            promote bone formation in a rat model of age-related bone loss
  JOURNAL   FASEB J 34 (9), 11754-11771 (2020)
   PUBMED   32652777
  REMARK    GeneRIF: Low-magnitude vibration induces osteogenic differentiation
            of bone marrow mesenchymal stem cells via miR-378a-3p/Grb2 pathway
            to promote bone formation in a rat model of age-related bone loss.
REFERENCE   2  (bases 1 to 2608)
  AUTHORS   Lin,X., Gu,Y., Kapoor,A., Wei,F., Aziz,T., Ojo,D., Jiang,Y.,
            Bonert,M., Shayegan,B., Yang,H., Al-Nedawi,K., Major,P. and Tang,D.
  TITLE     Overexpression of MUC1 and Genomic Alterations in Its Network
            Associate with Prostate Cancer Progression
  JOURNAL   Neoplasia 19 (11), 857-867 (2017)
   PUBMED   28930697
REFERENCE   3  (bases 1 to 2608)
  AUTHORS   Ge,S., Xiong,Y., Wu,X., Xie,J., Liu,F., He,J., Xiang,T., Cheng,N.,
            Lai,L. and Zhong,Y.
  TITLE     Role of growth factor receptor-bound 2 in CCl4-induced hepatic
            fibrosis
  JOURNAL   Biomed Pharmacother 92, 942-951 (2017)
   PUBMED   28618656
  REMARK    GeneRIF: GRB2 plays an important role in CCl4-induced hepatic
            fibrosis by regulating hepatic stellate cell function, and
            up-regulation of GRB2 induced by HMGB1 is mediated via the PI3K/AKT
            pathway.
REFERENCE   4  (bases 1 to 2608)
  AUTHORS   Manu,M.S., Rachana,K.S. and Advirao,G.M.
  TITLE     Altered expression of IRS2 and GRB2 in demyelination of peripheral
            neurons: Implications in diabetic neuropathy
  JOURNAL   Neuropeptides 62, 71-79 (2017)
   PUBMED   28065675
  REMARK    GeneRIF: a radical increase in the phosphorylation of IRS2 at
            serine 731 prevents the recruitment of GRB2 adaptor protein which
            may fail further to connect the Ras and other pathways required to
            the cell for its survival and to maintain integrity
REFERENCE   5  (bases 1 to 2608)
  AUTHORS   Xia,Y., Sheng,J., Liang,G.Y., Liu,D.X., Tang,Q. and Cheng,A.P.
  TITLE     miR-96 inhibits cardiac hypertrophy by targeting growth factor
            receptor-bound 2
  JOURNAL   Genet Mol Res 14 (4), 18958-18964 (2015)
   PUBMED   26782545
  REMARK    GeneRIF: Growth factor receptor-bound 2 is a direct target of
            miR-96, which is a negative regulator of cardiac hypertrophy.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 2608)
  AUTHORS   Watanabe,K., Fukuchi,T., Hosoya,H., Shirasawa,T., Matuoka,K.,
            Miki,H. and Takenawa,T.
  TITLE     Splicing isoforms of rat Ash/Grb2. Isolation and characterization
            of the cDNA and genomic DNA clones and implications for the
            physiological roles of the isoforms
  JOURNAL   J Biol Chem 270 (23), 13733-13739 (1995)
   PUBMED   7775428
REFERENCE   7  (bases 1 to 2608)
  AUTHORS   Jackman,J.K., Motto,D.G., Sun,Q., Tanemoto,M., Turck,C.W.,
            Peltz,G.A., Koretzky,G.A. and Findell,P.R.
  TITLE     Molecular cloning of SLP-76, a 76-kDa tyrosine phosphoprotein
            associated with Grb2 in T cells
  JOURNAL   J Biol Chem 270 (13), 7029-7032 (1995)
   PUBMED   7706237
REFERENCE   8  (bases 1 to 2608)
  AUTHORS   Sasaoka,T., Langlois,W.J., Leitner,J.W., Draznin,B. and
            Olefsky,J.M.
  TITLE     The signaling pathway coupling epidermal growth factor receptors to
            activation of p21ras
  JOURNAL   J Biol Chem 269 (51), 32621-32625 (1994)
   PUBMED   7798267
REFERENCE   9  (bases 1 to 2608)
  AUTHORS   Suen,K.L., Bustelo,X.R., Pawson,T. and Barbacid,M.
  TITLE     Molecular cloning of the mouse grb2 gene: differential interaction
            of the Grb2 adaptor protein with epidermal growth factor and nerve
            growth factor receptors
  JOURNAL   Mol Cell Biol 13 (9), 5500-5512 (1993)
   PUBMED   7689150
REFERENCE   10 (bases 1 to 2608)
  AUTHORS   Matuoka,K., Shibata,M., Yamakawa,A. and Takenawa,T.
  TITLE     Cloning of ASH, a ubiquitous protein composed of one Src homology
            region (SH) 2 and two SH3 domains, from human and rat cDNA
            libraries
  JOURNAL   Proc Natl Acad Sci U S A 89 (19), 9015-9019 (1992)
   PUBMED   1384039
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AA926364.1 and BC091144.1.
            
            On or before Dec 15, 2013 this sequence version replaced
            XM_006247767.1, XM_003750955.1, XM_003750956.1, NM_030846.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            RNAseq introns :: single sample supports all introns SAMEA5760393,
                              SAMEA5760396 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-428               AA926364.1         1-428
            429-2608            BC091144.1         373-2552
FEATURES             Location/Qualifiers
     source          1..2608
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="Sprague-Dawley"
                     /db_xref="taxon:10116"
                     /chromosome="10"
                     /map="10q32.1"
     gene            1..2608
                     /gene="Grb2"
                     /gene_synonym="Ash-psi"
                     /note="growth factor receptor bound protein 2"
                     /db_xref="GeneID:81504"
                     /db_xref="RGD:619758"
     exon            1..61
                     /gene="Grb2"
                     /gene_synonym="Ash-psi"
                     /inference="alignment:Splign:2.1.0"
     exon            62..265
                     /gene="Grb2"
                     /gene_synonym="Ash-psi"
                     /inference="alignment:Splign:2.1.0"
     CDS             188..841
                     /gene="Grb2"
                     /gene_synonym="Ash-psi"
                     /note="protein Ash; SH2/SH3 adapter GRB2; adapter protein
                     GRB2; growth factor receptor-bound protein 2-like"
                     /codon_start=1
                     /product="growth factor receptor-bound protein 2"
                     /protein_id="NP_110473.2"
                     /db_xref="GeneID:81504"
                     /db_xref="RGD:619758"
                     /translation="MEAIAKYDFKATADDELSFKRGDILKVLNEECDQNWYKAELNGK
                     DGFIPKNYIEMKPHPWFFGKIPRAKAEEMLSKQRHDGAFLIRESESAPGDFSLSVKFG
                     NDVQHFKVLRDGAGKYFLWVVKFNSLNELVDYHRSTSVSRNQQIFLRDIEQVPQQPTY
                     VQALFDFDPQEDGELGFRRGDFIHVMDNSDPNWWKGACHGQTGMFPRNYVTPVNRNV"
     exon            266..363
                     /gene="Grb2"
                     /gene_synonym="Ash-psi"
                     /inference="alignment:Splign:2.1.0"
     exon            364..486
                     /gene="Grb2"
                     /gene_synonym="Ash-psi"
                     /inference="alignment:Splign:2.1.0"
     exon            487..655
                     /gene="Grb2"
                     /gene_synonym="Ash-psi"
                     /inference="alignment:Splign:2.1.0"
     exon            656..2581
                     /gene="Grb2"
                     /gene_synonym="Ash-psi"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 cggccgcggg cggcagcaca aggcgggcca ggccgggtga gggagcggac ggcacgtcgc
       61 gggtggcatt gtgtgtccca gtgtgcaaga atagccccag aagaggaaag gctgagccca
      121 gagcgcttca gcagggaaga ttcccttccc cccgcttcag gctgctgagc actgagcggc
      181 gctcagaatg gaagccatcg ccaaatatga cttcaaagct actgctgacg atgagctgag
      241 cttcaagagg ggggacatcc ttaaggtttt gaatgaagag tgtgatcaga actggtataa
      301 ggcagaactc aatgggaaag atggcttcat ccccaagaat tacatagaaa tgaaaccaca
      361 tccgtggttt tttggcaaaa tccccagagc aaaggcagaa gaaatgctta gcaaacagcg
      421 gcatgacggg gccttcctga tccgagagag cgagagcgct cctggggatt tctccctgtc
      481 agtcaagttt ggaaatgatg tacagcactt caaggtgctt cgtgacggag ccgggaagta
      541 cttcctgtgg gtggtgaagt ttaattcttt gaatgagctg gtagattacc acagatcaac
      601 atccgtgtcc aggaaccagc agatattcct ccgggacata gaacaggtgc cacagcagcc
      661 aacgtacgtc caggcactct ttgactttga cccccaggag gatggtgagc tgggctttcg
      721 ccgaggagac ttcattcatg tcatggataa ctcagatccc aattggtgga aaggggcctg
      781 ccatgggcag accggcatgt ttccccgcaa ttatgtcacc ccagtgaacc ggaacgtcta
      841 agaagcaaaa gagattattt aaagaaagtg aaaagttgag accgttcaca agaattacac
      901 ccacacgctg cctgtcacag cctgtgaggg aacgcagaac acctggctgg gtcccacggg
      961 cgaccctctc acttaagttg caacttttgg gggtgggaag gggtgtttga tttcataatg
     1021 ccaaaactta acctattgaa tgaattacag tttttattac ggaatctcgc cgctactcgt
     1081 gttcccttcc tgtgtccttt tttcgcgttc tttctttcct gtccagtgca tgatgtttaa
     1141 ggccacatat agtccagctg atgccaataa taaaagaaaa gaaaccaagt gggctggtat
     1201 tttctctatg caaatgtctg tggagatgga tggactagag gagccggatt cttcacacac
     1261 aggggcagcc attgtatcgg ggcccttggt tgggattcac tggagatgcc taagggcatc
     1321 tgcctccggc ctgaagcctg gagcattcca tcagagttgg aattagggca ggagcctgct
     1381 gcccacccat ggtctcctga gagccagact gcagagctgc cctccccact gcctcccttc
     1441 agtgtctcac ttgttttcat agtgcctttg tcttatttca agggtgttca tgagtggtgt
     1501 ctttaccttt tttatttttt taaataagtt aaagatagtc cagagctttt cagttgatta
     1561 gtctcctatc ctatgtacat attttcctct cagggcagga gaaagaggac agagaaagga
     1621 gctggcagaa gcagagaggg tgtatttccc atcttaaatg ggccagaggt cttgaaggat
     1681 cagcttttac attgtgagct ttgacacctg cttcagctca gactcaccgg ccctcagagt
     1741 ccctgccctc caggtcccag ttgccctttc tatgcctaga gccactaagg gtcaaaacac
     1801 aaggcaggca ggggggagcg ggtggcatcc ttccctctca taggcctaca agcaggtgga
     1861 agttgaaaat tctggccttt aatttttctt gctcttacaa ttaagtcctc gttctcagtt
     1921 gcacatttcc ccccacctgt ccacgagtac gaatggcagg attgcggggc tggttgcatg
     1981 gttgcatctc tgtgcacaac agtggtgttt gcagctgctc tgctgcccca tcccatgaag
     2041 ccttttgagg tagaaaagac caagagcaac gcaaagcttt ttaaatcttg tctcctgtga
     2101 gctctcagtc ctccgtagtc atgtgcctgt ccaccgtttc tctccacgca gcctcctccc
     2161 cacagcagag tcatggctac tcccaggctc tcccttggag ttggcggcgt ccgtgggctg
     2221 ctggactctt gagggtgggg tcccccctgg actctgtttt gtgagatgga ctcagctcgg
     2281 agcacccctg aggccccgga ggcgtcctgg gcggagcctg ttcccacccc tcctggttct
     2341 gctgcctctt tccctgctga tgataaaaat ttatctctgg agttacacct gggccatttg
     2401 attgttttat tttggaattg gtgtatatca tgaagccttg ctgaactaag ttttgtgtgt
     2461 atatatttaa agatcagtgt ttaaataaaa aaacaaacct atgtacttac gcctctaact
     2521 tctgcgaata gcaatttggt aggtagtgat taactgtgaa taaactccaa tgacttcgta
     2581 aaaaaaaaaa aaaaaaaaaa aaaaaaaa
//
